Water in oil based PGPR formulation of Pseudomonas fluorescens (FP7) showed enhanced resistance against Colletotrichum musae

Banana anthracnose incited by Colletotrichum musae is a serious disease in post harvest marketing stage. It occurs in almost all the banana growing countries. In the current study, 5 isolates of C. musae infecting banana cv nendran were collected from various district of Tamil Nadu. C5 isolate collected from Tiruchirappalli showed maximum virulent with (66.60%) PDI. In vitro efficacy of six Pseudomonas fluorescens superior strains were tested for their inhibition on mycelial growth of C. musae. P. fluorescens (FP7) showed significantly higher inhibition of 41.12 per cent over control and with a mean mycelial growth of 52.10 mm. Water in oil emulsion was used in the present research for formulation of P. fluorescens (FP7). The emulsion contained the following ingredients (w/w), sterile deionized water (45.25%), glycerine (4.00%), water-soluble wax ((0.75%), Tween 20 (2.50%), and a mixture of 19.00% soybean oil + 28.50% castor oil. The application of water in oil emulsion formulation significantly increased the yield (68.12 t/ha) compared to control (66.49 t/ha), and also increase in the activity of defense related enzymes viz., phenyl alanine ammonia lyase, peroxidase, polyphenol oxidase, catalase and β-1, 3 glucanase was observed which was followed by talc based formulation of Pf1 and FP7.     

一株鸡新城疫病毒的分离鉴定及其油乳剂灭活苗免疫效果评价

从河南某种鸡场发病鸡群中分离出1株毒株,经HA、HI和动物回归试验,确定分离的病毒为NDV,命名为HNYD株。经过测定,HNYD株鸡胚半数致死量（ELD50）为10^90/0．1mL;鸡胚最小致死量的平均致死时间（MDT）为53．8h;对1日龄SPF雏鸡脑内致病指数（ICPI）为1．86,提示HNYD株为强毒力株。血清交叉反应试验显示HNYD株和La Sota株存在一定的抗原性差异。用HNYD株油乳剂灭活苗对HNYD株和La Sota株的保护率分别为90％和100％,明显优于La Sota株油乳剂灭活苗,提示HNYD株制备的疫苗对流行毒株和经典毒株的攻击均能很好的保护。     

高效氯氰菊酯微乳剂液相色谱分析

本文介绍了从高效氯氰菊酯微乳剂中, 分离出有效成分高效氯氰菊酯, 再用正相液谱定量检测。该方法的变异系数为０．５１％ ,平均回收率为９９．９６％ 。     

蓖麻油聚氨酯丙烯酸酯复合乳液的研制

采用甲基丙烯酸甲酯(MMA)与蓖麻油水性聚氨酯共聚反应制备蓖麻油聚氨酯丙烯酸酯(CPUA)复合乳液,研究了蓖麻油添加量、MMA添加量和引发剂种类对合成CPUA复合乳液及涂膜性能的影响,并应用傅立叶红外光谱(FT-IR)测定反应产物的结构.研究发现随着蓖麻油和MMA添加量增大,CPUA复合乳液胶粒平均粒径增大,粘度减小,涂膜光泽度下降,机械性能和耐水性增强,确定了合适的蓖麻油和MMA含量,分别为体系总固体质量的20%～25%和20%～30%.采用偶氮二异丁腈(AIBN)和过硫酸钾(KPS)复合引发剂可提高单体的转化率;性能测试表明,本研究合成的CPUA复合乳液具有优异的物理机械性能和耐水性.     

Impact of Wall Material on the Physiochemical Properties and Oxidative Stability of Microencapsulated Spray Dried Silver Carp Oil

This study aimed to microencapsulate silver carp oil by spray drying with different combinations of wall materials (gum Arabic, modified starch Hi-cap, maltodextrin, and inulin) and evaluate the effects of these combinations on the physiochemical and oxidative stability of the microcapsules. Feed emulsions were also investigated prior to spray drying. Modified starch-based microcapsules had higher encapsulation efficiency (91.22-92.78%) and yield (87.41-92.15%), excellent solubility (91.85-93.67%), good particle size (301.20-407.73 d.nm), color, and powder morphology. The presence of inulin reduced agglomeration in powders. All the powders exhibited good protection against lipid oxidation during 2 months of storage, whereas the modified starch and inulin combination provided the best stability.     

大豆亲脂蛋白-甲基纤维素W/O/W乳液稳定性研究

采用大豆亲脂蛋白（Soybean lipophilic protein, LP）和甲基纤维素（Methyl cellulose, MC）的复合物作为亲水性乳化剂形成W/O/W乳液外水相,以聚蓖麻酸甘油酯（Polyglycerol polyricinoleate, PGPR）为亲脂性乳化剂,橄榄油为油相,并以维生素B₁₂为指示剂,按两步法制备W/O/W型双层乳液。通过对微观结构、储藏稳定性、粒径、电位、乳液粘弹性等指标的测定,探究不同外水相比例及复合物中LP与MC的不同比例对W/O/W乳液稳定性的影响。实验结果表明：当LP与MC质量比为3∶1,外水相质量分数为70%时,W/O/W乳液液滴间的作用力最强、粒径最小、Zeta-电位绝对值最大、对活性物质的保护作用最强、28 d储藏稳定性指数较高,流变学性质较好。     

5%噻虫嗪水乳剂防治稻飞虱田间药效试验

采用5%噻虫嗪水乳剂防治稻飞虱田间药效试验。结果表明,5%噻虫嗪水乳剂有效成份用量为15.00、11.25、7.50 g/hm2对稻飞虱的防治效果均显著优于对照药剂25%噻虫嗪水分散粒剂有效成份用量7.5 g/hm2,药后第1天防效高达90.34%,药后第3天防效高达95.03%,药后第7天防效高达92.43%,且在试验作物上未见药害发生。     

20%乙氧氟草醚水乳剂的研制

本文介绍了20％乙氧氟草醚水乳荆配方的组成和制备方法,并对其性能和田间药效进行了测试和试验。表明该水乳剂成本较低、药效好、使用安全,具有良好的开发前景。     

生防菌Alternaria amaranthi-3对反枝苋的防治效果

反枝苋Amaranthus retroflexus是一种世界性恶性杂草。为了确定微生物除草剂候选菌Alternaria amaranthi-3防除反枝苋的潜力,通过盆栽试验研究了接种浓度、露期和水乳剂型对A.ama-ranthi-3菌株致病力的影响。结果显示,接种浓度显著影响菌株的致病力,在48h露期条件下,接种孢子浓度为105个/mL时,菌株水剂对反枝苋幼苗生长抑制率为35.55%;浓度为107个/mL时,生长抑制率达到75.25%。露期对该菌株的致病力也有较大影响,在不保湿条件下,菌株水剂对反枝苋的生长抑制率为26.43%,而保湿48h处理的生长抑制率达到77.96%。Span80∶Tween80=1∶3的复配乳化剂和大豆油制备的水乳剂可显著降低露期对菌株防效的影响和提高菌株的致病力,无露期时,菌株水乳剂对反枝苋的生长抑制率达到88.35%,显著高于水剂;48h露期条件下,菌株水乳剂处理的生长抑制率为90.59%,而菌株水剂处理为77.96%。表明通过剂型的改进菌株Alternaria amaranthi-3能有效防除反枝苋。     

中温高黏度介质双端面主密封热力特性分析

为研究中温高黏度介质双端面机械密封主密封的传热和变形规律以及冲洗液的压力和温度对其影响,从而为密封性能改进和冲洗液的高效控制提供依据.以乳液输送设备双端面机械密封(AR201)为研究对象,建立传热计算模型,采用有限元法分析主密封温度场,研究了密封环温度和变形量随冲洗液压力和温度的变化规律.研究表明:密封环端面的最高温处于动静环接触面靠近密封腔位置处,在冲洗液的作用下,动静环内径侧温度明显低于外径侧,动静环温度自接触面开始沿轴向至环外侧逐渐降低;端面最高温度和最大变形量随冲洗液温度和压力的升高而增大,最大变形量的大小和随冲洗液参数变化的波动幅度与密封材料有关;合理选择冲洗液压力和温度是有效改善主密封性能的措施,对于AR201机械密封,将冲洗液压力控制在0.80~0.85 MPa和较低的温度能减少端面磨损现象.