Identification and breeding behaviour of a major deletion of chromosome 5D ofTriticum aestivum

Summary A study was undertaken to evaluate the breeding behaviour and to identify a spontaneously produced putative chromosomal deletion in the winter wheat (Triticum aestivum L. em. Thell.) cv Norstar. Male and female transmission studies of plants heterozygous for the deletion chromosome indicated 9.5% and 48.8% transmission through the pollen and the egg, respectively. Meiotic analyses of progeny from deletion heterozygotes indicated that the deletion chromosome was eliminated from half of the plants, which agreed with the male and female transmission frequencies. Testcrosses of the deletion chromosome with telocentrics and nullisomic-tetrasomic combinations suggested that the deletion involved the long arm of chromosome 5D. This was confirmed by restriction fragment length polymorphism (RFLP) analysis. Also, monosomic plants obtained in progeny of deletion heterozygotes were shown to be monosomic for 5D. Studies of plants homozygous for the deletion showed relatively normal pairing between the deletion chromosomes, and with the short arm (5DS), but not the long arm (5DL). Deletion homozygotes were self-sterile, and morphologically similar to plants nullisomic for 5D, but plants that also contained 5DL, or a homoeologous chromosome were self-fertile and had normal morphology. Studies of chromosome morphology indicated that the deletion chromosome was metacentric, and the length of the long arm was reduced by approximately 60%. RFLP studies showed that, in terms of genetic distance, 90% of the arm was missing.     

Utilization of deletions to localize a gene for resistance to the cereal cyst nematode, Heterodera avenue, on an Aegilops ventricosa chromosome

A previous RFLP analysis showed that the Aegilops ventricosa chromosome 6M^V which compensates for the absence of 6D in 6M^V (6D) wheat substitution lines was a 2/6 translocated chromosome, either 2S–6S.6L or 2S–6L.6S. The distal part of its long arm consists of a translocated segment belonging to homoeologous group 2. Chromosome 6M^V carries a gene(s) for resistance to cereal cyst nematode. In order to define the part of 6M^V (2S or 6S or 6L) involved in this resistance, addition lines with a 6M^V deleted either for its short arm or for the distal part of its long arm were evaluated. It was shown that the gene(s) is carried by the group 2 translocated segment. The hypothesis that the gene(s) could be allelic to Cre2, another gene conferring resistance to the nematode introduced into the wheat complement from Ae. ventricosa is discussed.     

The Use of Multivariate Analyses to Eliminate Redundant Variables when Evaluating Potatoes for Chip Quality

Selection for good chipping clones in potato breeding often takes place during the middle and late selection stages. This is due to the considerable resources needed for the assessment of several characteristics. Objective multivariate methods for the elimination of redundant variables were applied to eight standardized chipping quality traits recorded on advanced clones over 15 years at two locations in New Brunswick, Canada. The clones were evaluated of specific gravity and the following chipping scores: direct from the field in August; after 10 days storage at 13 °C; from 4 °C storage in February; from 4 °C storage in February with 1 week, 2 weeks, 3 weeks and 4 weeks reconditioning at 16 °C. Five elimination methods produced a similar deletion order. In every subset obtained, specific gravity and direct chipping from the field were always retained. Reconditioning treatments from one to four weeks after cold storage were shown to be the most redundant. The reduction in the number of traits from eight to four by every technique could recover more than 80 % of the original variation, showing that redundant variables were effectively discarded.     

衍生金融工具及其在企业财务管理中的作用

衍生金融工具是在汇率、利率、股票、债券和商品等交易工具的基础上衍生出来的，是金融创新的产物，有其自身的特点。该文阐述了衍生金融工具在国际金融市场上的产生背景、发展历史及在我国的发展状况，并对一些常见衍生金融工具进行了简要介绍。通过分析衍生金融工具的若干共性，指出其在企业融资、投资方面所能发挥的作用。     

变型拖拉机转向梯形机构参数最佳值的确定

为了解决变型拖拉机转向性能差等问题，采取建立转向车轮实际转向角的数学模型，计算机辅助分析方法，可以确定转向梯形机构参数的最佳值，从而较好地解决了转向性能差等问题．     

多肉植物塑化标本制作研究

本文从“永生花”的存在提出“永生多肉”的想法，既保存其鲜活性，又不让它丧失独特的肉感美，本研究探讨了多肉植物固色、塑化和环氧树脂AB胶包埋的工艺，建立了适合多肉植物的标本制作方法。主要不同叶片厚度的多肉植物利用硫酸铜溶液浸泡法及干燥法进行固色，结果显示10%硫酸铜溶液固色96h，对叶片中、厚型绿色多肉效果最佳，硅胶干燥法适合叶片薄型多肉植物。按25%-50%-75%-100%的浓度梯度PEG200脱水塑化，每个浓度24h，效果最好。最后以环氧树脂AB胶结合UV胶进行定型包埋。     

四川冕宁县缺硒饲粮对雏鸡的影响及不同品种雏鸡适宜补硒量的研究

采用四川严重缺只硒地区冕宁县实用雏鸡饲粮饲喂泸宁鸡(当地地方鸡)、白洛克和来杭三个品种共682只雏鸡,作了两个试验,结果表明:谷实类缺硒饲粮(含硒0.0051ppm)引起雏鸡严重营养性胰腺萎缩和纤维化;泸宁鸡和白洛克缺硒死亡率分别为83％和63％,添加维生素E可起到轻微缓解作用(缺硒死亡率52％);补硒显著促进生长和改善饲料利用率。饲粮中添加动物性饲料(使基础日粮含硒0.1018ppm)后雏鸡未出现明显缺硒病变,但补硒显著提高谷胱甘肽过氧化物酶(GSH-px活力、组织硒含量和生长速度。雏鸡对缺硒的敏感程度、组织硒含量和GSH-px活力存在明显品种差异。以GSH-px活力和组织硒含量作为评定标准结合对生长速度的考察表明:对于生长较快的泸宁鸡和白洛克,基础饲粮补加0.20ppm硒是适宜的,而对于蛋用型来杭鸡则以添加0.10ppm效果较好。     

同源重组探针在暂时转化的烟草原生质体内的重组和缺失

通过电激法(electroporation)将同源重组探针pBC 7导入烟草原生质体,再从转化的烟草原生质体中提取DNA,并将其转化到E.coli DH 1细胞.结果在E.codi DH 1细胞中获得了一种新的质粒分子,该质粒含有功能的neo基因,与重组探针pBC 7相比有较大的缺失、倒位,并出现了新的限制酶切部位,重组探针pBC 7的主要特征为含有Tn5的neo基因两个截短的不等部份,只有通过重排才可能产生完整的neo基因,这表明了暂时转化的原生质体在外源DNA整合以前具有这样的基因重排功能。     

ipt基因促进矮牵牛遗传转化效率及热激启动子驱动基因删除

本研究主要探讨ipt基因对矮牵牛遗传转化不定芽诱导影响及拟南芥热激启动子hsp18.2驱动重组酶基因flp的热诱导外源基因删除表达载体在矮牵牛中的基因删除效果。本研究中将植物表达载体pBin-hsp18.2：flp-35S：ipt及对照载体pBin-hsp18.2：flp遗传转化矮牵牛,以获得转基因植株,分析比较不定芽的诱导和转基因植株进行的热激基因删除。研究结果表明,ipt基因可促进矮牵牛遗传转化过程中不定芽的诱导,其不定芽诱导率为21.5%,显著高于对照的8.7%。在44℃,6h,热激6次的条件下,转基因矮牵牛植株表型恢复正常,经GUS蛋白表达分析及PCR、RT-PCR检测,证明外源基因已经被删除。转基因矮牵牛基因删除效率最高可达43.8%。本研究为ipt基因在一些遗传转化困难植物转基因中的应用奠定了基础。     

The phytotoxicity of natural tetramic acid derivatives

Natural tetramic acid derivatives have attracted a great deal of interest of chemists because of their structural features and the broad range of biological activities. This paper focuses on five naturally occurring tetramic acid derivatives reported to have phytotoxicity and discusses their common structural characteristics. Copyright © 2011 Society of Chemical Industry