Aux/IAA基因家族鉴定及对大豆茎尖发育的调控作用分析

Aux/IAA 基因家族在植物茎尖发育过程发挥重要作用。为了探究大豆Aux/IAA 基因家族在大豆茎尖发育过程的调控作用，本文以拟南芥Aux/IAA 基因家族蛋白序列为参照鉴定了大豆全基因组Aux/IAA 家族基因，包括63个成员；然后以拟南芥、鹰嘴豆和大豆的Aux/IAA 家族为研究对象，比对这些基因全长氨基酸序列并构建进化树，结果表明三种作物的Aux/IAA 家族成员间亲缘关系差异明显，进化过程中同源重组频率不同；进而利用RNA-seq技术分析东农594（DN）、Charleston（CH）及二者杂交后代的RIL群体的高矮秆池（WH、WS）和F2群体的高矮秆池（JH、JS）的差异表达基因及其功能，共有17个Aux/IAA 基因在3组材料中差异表达。CHvsDN组有15个差异表达基因，JHvsJS组有2个，WHvsWS组只有1个；其中，Glyma.10G180100 在JHvsJS组和WHvsWS组均差异表达，这些结果为进一步研究大豆Aux/IAA 基因家族的功能及调控茎尖发育提供理论依据。     

平菇培养料发酵过程理化因素及微生物变化研究

通过对平菇(Pleurotus ostreatus)玉米芯培养料进行发酵处理,监测玉米芯发酵料的堆温和pH值变化,对不同发酵阶段进行微生物区系分析并测定了发酵过程中脱氢酶和碱性磷酸酶的变化。结果表明,在玉米芯培养料的发酵过程中,堆温先升高后降低,而pH值先降低后升高。微生物主要以细菌和放线菌为主,真菌数量很少。随着发酵的进行,细菌和放线菌数量,脱氢酶以及碱性磷酸酶的活性呈逐渐上升趋势。     

Integration of soil applied neonicotinoid insecticides and acibenzolar-S-methyl for systemic acquired resistance (SAR) control of citrus canker on young citrus trees

Soil application of systemic neonicotinoid insecticides for control of psyllid vectors of Huanglongbing disease on young citrus trees also produces season-long SAR control of citrus canker caused by Xanthomonas citri subsp. citri Schaad et al. The neonicotinoids imidacloprid (IMID) and thiamethoxam (THIA) were compared with soil or sprinkler applications of the commercial SAR inducer acibenzolar-S-methyl (ASM) and foliar sprays of copper hydroxide (CH) and/or streptomycin (STREP) to evaluate their effects on the percentage of canker-infected leaves on 2-yr-old ‘Vernia’ orange and 3-yr-old ‘Ray Ruby’ grapefruit trees in Southeast Florida. All treatments significantly reduced the incidence of foliar canker compared to the untreated check. Soil drenches of ASM and season long rotations with IMID and THIA were highly effective for suppressing foliar canker on young grapefruit and orange trees under weather conditions absent of high intensity rains or tropical storms. Sprinkler application of ASM was less effective than soil drench. The level of control for SAR treatments was comparable to eleven 21-day interval sprays of CH and/or STREP. SAR induced by soil-applied insecticides provides substantial benefits for canker disease management on young citrus trees that may be augmented with ASM.     

Seasonal Variations in Physicochemical and Textural Properties of North Atlantic Cod (Gadus morh.ua) Mince

Abstract

Seasonal variations in physicochemical and textural properties of minced cod muscle from Icelandic coastal areas were evaluated. Measurements were performed on mince from the white muscle. Compared to the winter and summer values, hardness and cohesiveness, as measured by Instron, showed a drop in March to May and another drop was observed for cohesiveness during the autumn months. Expressible moisture changed slightly from March to August whereas results from the winter months indicate greater fluctuations. A minimum was observed for protein solubility in March and July. The surface hydrophobicity of proteins, increased gradually from November to May followed by a sudden drop, then a gradual increase again.     

栀子花化学成分的GC/MS分析

采用超声波提取栀子花提取液,然后对其化学成分进行GC/MS分析。从栀子花中分离确定出27种主要成分,其中萜类物质含量相对较高。     

Impact of organic residues and mineral fertilizer application on soil–crop system: II soil attributes

The effects of crop residues alone (Cr⁺) or combined with inorganic fertilizer (FCr⁺) sewage sludge (SS) and humentos (H) on soil properties were studied in a five-season wheat–guar rotation. Incorporation of Cr⁺ and SS significantly decreased pH by 5–9%. Highest organic carbon (OC) was observed in SS plots, whereas Cr⁺ resulted in values threefold that of the control. The added effect of Cr⁺ (46%) combined with fertilizer on total nitrogen (TN) was greater than Cr⁺ by 28%. The fraction of total residue N mineralized was 71%, whereas net N mineralized in Cr⁺, SS, H and FCr⁺ plots at 0–20 and 20–40 cm depths were 39.8, 52.3, 11.4, 66.7 mg kg^?1 and 21.2, 27.9, 8.2, 41.2 mg kg^?1, respectively. Integrated application of Cr⁺ with fertilizer had a synergistic effect on P content (3.49–3.69 units), but soil levels has never exceeded 13 mg P kg ^?1. Sole Cr⁺ significantly increased topsoil cation-exchange capacity (CEC) compared with the control treatment (9.39–21.59%). Application of SS, FCr⁺ and Cr⁺ significantly increased water-holding capacity and decreased bulk density by 9–15% and 8–9%, respectively, suggesting that such practice can restore the productivity of degraded soils and improve crop yields.     

Study on the Protection of Citizen＇ s Cultural Right in Public Library Regulation

公共图书馆作为公共文化服务的基础设施之一,有义务承担起保护公民文化权利的责任。公共图书馆制度为满足公民获得文化权利提供了制度基础,是保障公民文化权利的制度安排。通过探讨文化权利的含义及保障公民文化权利的重要意义,提出了保障公民文化权利是公共图书馆的职责之一,明确了公共图书馆在那些方面保障公民的文化权利。     

KEYSTONE生态护坡砖在广州东濠涌整治工程中的应用

生态护岸是结合植物与土木工程对河道坡面进行防护的一种新形式。生态护岸集防洪效应、生态效应、景观效应和自净效应于一体,是生态文明时代河道护岸技术的发展方向。文章概述了目前国内生态河道护坡构建技术的发展现状,并对KEYSTONE生态河道护坡砖在广州东濠涌综合整治工程中的应用进行了探讨研究。     

草坪常用化学药剂对昆虫病原线虫存活和侵染率的影响

测定了草坪上常用的14种化学药剂对蝼蛄斯氏线、长尾斯氏线虫X-7品系和小卷蛾斯氏线虫All品系存活和侵染率的影响。杀虫剂乙酰甲胺磷在高浓度和推荐浓度下对蝼蛄斯氏线虫、长尾斯氏线虫和小卷蛾斯氏线虫的致死率分别为21％和9．5％，13％和5．5％，5．5％和2％；敌百虫在高浓度和推荐浓度下对蝼蛄斯氏线虫的致死率分别高达94．5％和92．5％，在高浓度下对长尾斯氏线虫的致死率为8％，对小卷蛾斯氏线虫没有明显致死作用；克蛾宝对3种线虫的致死作用均比乙酰甲胺磷和敌百虫低。杀菌剂对3种线虫的存活均无影响。除草剂2，4-D丁酯对3种线虫低浓度和高浓度下致死率分别为2％、6％、3．5％和5％、85．5％、6％。经化学药剂处理24h后的线虫侵染黄粉虫9-11龄幼虫，对线虫存活没有影响或者影响较小的化学药剂对线虫的侵染率都没有明显的影响，但剂型为10％可湿性粉剂的吡虫啉对长尾斯氏线虫，以及高浓度的2，4-D丁酯对小卷蛾斯氏线虫的侵染率有明显抑制作用。