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41.
Comparative,multidimensional imaging of patent ductus arteriosus and a proposed update to the morphology classification system for dogs 下载免费PDF全文
42.
Evaluation of individual low‐dose dexamethasone suppression test patterns in naturally occurring hyperadrenocorticism in dogs 下载免费PDF全文
43.
Effect of dasatinib in a xenograft mouse model of canine histiocytic sarcoma and in vitro expression status of its potential target EPHA2 下载免费PDF全文
K. Ito R. Miyamoto H. Tani S. Kurita M. Kobayashi K. Tamura M. Bonkobara 《Journal of veterinary pharmacology and therapeutics》2018,41(1):e45-e48
Canine histiocytic sarcoma (HS) is an aggressive and highly metastatic tumor. Previously, the kinase inhibitor dasatinib was shown to have potent growth inhibitory activity against HS cells in vitro, possibly via targeting the EPHA2 receptor. Here, the in vivo effect of dasatinib in HS cells was investigated using a xenograft mouse model. Moreover, the expression status of EPHA2 was examined in six HS cell lines, ranging from insensitive to highly sensitive to dasatinib. In the HS xenograft mouse model, dasatinib significantly suppressed tumor growth, as illustrated by a decrease in mitotic and Ki67 indices and an increase in apoptotic index in tumor tissues. On Western blot analysis, EPHA2 was only weakly detected in all HS cell lines, regardless of sensitivity to dasatinib. Dasatinib likely results in the inhibition of xenograft tumor growth via a mechanism other than targeting EPHA2. The findings of this study suggest that dasatinib is a targeted therapy drug worthy of further exploration for the treatment of canine HS. 相似文献
44.
Repeat patient testing shows promise as a quality control method for veterinary hematology testing 下载免费PDF全文
Bente Flatland Kathleen P. Freeman 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(2):252-266
Background
Repeat patient testing‐based quality control (RPT‐QC) is a potential method for veterinary laboratories (eg, that have a limited budget for quality commercial control material [QCM] or that wish to use material with a species‐specific matrix).Objectives
To determine whether total error (TEa), probability of error detection (Ped), and probability of false rejection (Pfr) similar to that achievable with QC materials can be controlled using RPT‐QCMethods
Control limits (WBC, RBC, HGB, HCT, MCV, and PLT) for the Advia 120 (n = 23) and scil Vet ABC (n = 22) were calculated using data from normal canine specimens from a routine caseload. Specimens were measured at accession and again after 24 hours. Control limits were validated using 23 additional canine specimens tested similarly. Achievable TEa, Ped, and Pfr were investigated using the Westgard EZRules3 and compared to those achievable with commercial QCM.Results
Theoretical performance of RPT‐QC and commercial QCM‐QC are similar for 1‐3s with both n = 1 and 1‐3s with n = 2 for all measurands and both instruments. Achievable TEa values for RPT‐QC were close to ASVCP recommendations for most measurands; exceptions were PLT (both instruments) and WBC (scil Vet ABC).Conclusions
Repeat patient testing‐based quality control advantages include a species‐specific matrix, low‐cost, and absence of QC material deterioration over time (since a fresh specimen is used each day). A potential disadvantage is daily access to normal canine specimens. A challenge is determining control limits, which has a subjective element. Further study is needed to confirm actual RPT‐QC performance and to determine if RPT‐QC with abnormal patient specimens is feasible. 相似文献45.
46.
Evaluation of vegetable protein in canine diets: Assessment of performance and apparent ileal amino acid digestibility using a broiler model 下载免费PDF全文
D. C. Fiacco J. A. Lowe J. Wiseman G. A. White 《Journal of animal physiology and animal nutrition》2018,102(1):e442-e448
Recent technological advances in the human food industry with respect to meat processing have decreased the availability of animal proteins to the pet food industry which typically formulates diets with an excess of animal protein. In the long term, this is not sustainable; thus, alternative protein sources need to be investigated. This study examined three canine diets, comparing a typical animal protein‐based diet (control) with two experimental diets where the animal protein was substituted in part with vegetable protein (formulated based either on total protein or amino acid content) using a broiler model. Each diet was fed to six cages each containing two birds from day 15, 18 cages in total (36 birds). Excreta were collected from days 19 to 21. On day 23, birds were euthanized and weighed, and their ileal digesta were collected and pooled for each cage. In addition, one leg per cage was collected for evaluation of muscle mass. Results showed no significant difference in animal performance (feed intake or live weight gain) or muscle to leg proportion across the diets. Birds fed the control diet and the diet balanced for amino acid content exhibited the greatest coefficients of apparent metabolizability for nitrogen (p < .001). Birds fed the diets that contained partial replacement of animal with vegetable protein generally had greater ileal digestibility of amino acids compared to birds fed the control (animal protein) diet. Analysis of excreta showed no dietary difference in terms of dry matter content; however, birds fed the diet balanced for total protein and the diet balanced for amino acid content had significantly greater excreta nitrogen than the control (p = .038). Overall, the study suggests vegetable proteins when formulated based on amino acid content are a viable alternative to animal proteins in canine diets. 相似文献
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Soo-Young CHOI Woo-Chang JEONG Young-Won LEE Ho-Jung CHOI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):239-244
Contrast enhanced ultrasound (CEUS) is useful to evaluate tissue perfusion in the kidney. In veterinary
medicine, sedation or anesthesia may be required in uncooperative or panting patients. The aim of this study
was to evaluate and compare the normal kidney perfusion patterns in conscious and anesthetized dogs using
CEUS. Eight healthy beagles were used in this study. Scanning was performed in conscious dogs using manual
restraint (conscious group), or under general anesthesia using tiletamine-zolazepam and medetomidine (TZM
group) or medetomidine (M group). The contrast agent (Sonovue®) was administered as an IV bolus.
The peak intensity (PI), time to peak enhancement from injection (TTP0) and the time to peak
enhancement from the initial rise (TTPup), upslope, downslope and area under the curve (AUC) were
analyzed. Compared to the cortical values in the conscious group, TTP0 was significantly delayed in
the TZM group, and upslope, TTP0 and TTPup were significantly different in the M group.
The AUCs in the TZM and M groups were not different from those in the conscious group. The upslope of renal
medullary perfusion was significantly decreased in the TZM and M groups. TTP0 and TTPup
were also significantly delayed in these groups. The AUC of the medulla was significantly decreased in the M
group. Therefore, TZM is useful as an anesthetic protocol when performing CEUS, and the obtained data may
serve as reference values in the evaluation of renal perfusion using CEUS in dogs under anesthesia. 相似文献