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81.
82.
Twomey DF Griffiths PC Horigan MW Hignett BC Martin TP 《Veterinary journal (London, England : 1997)》2006,171(3):574-576
An outbreak of upper respiratory tract disease was investigated in a group of 17 housed home-bred calves on a mixed dairy, beef and sheep farm in Devon. Conjunctival swabs were collected and tested for Chlamydophila spp. DNA using a PCR test that detects Chlamydophila abortus and Chlamydophila psittaci. Six of the calves tested gave a positive result. Further epidemiological observations and laboratory testing indicated that the adult dairy cows, from which the affected calves originated, were the most likely source of infection. 相似文献
83.
Survey and analysis of common diseases of laboratory monkeys are great significance to understand disease epidemiology, and formulate a set of scientific and reasonable measures for prevention and control disease, to improve the quantity and quality of laboratory monkeys, to ensure the accuracy of the animal experiment results. Through the investigation of the sick and dead animals of a large-scale laboratory monkeys breeding facility in Kunming area in 2014, the animals were classified depends on the main diseases, and the diseases were classified according to adult and juvenile animals, data were analyzed by Excel Office 2010 software. The results showed the common diseases of laboratory monkeys included the digestive system, respiratory system, trauma, reproductive system, and locomotor system disease, which were 47.45%, 13.06%, 12.10%, 11.46% and 8.92% separately; Common diseases included diarrhoea, soft tissue injuries, abortion, lobar pneumonia, dysentery, arthritis, intestinal pneumatosis, chronic colitis, and extreme bad nutrition, the ratio were 18.47%, 11.15%, 8.91%, 8.28%, 8.28%, 7.96%, 5.10%, 5.10% and 5.09% separately. Comprehensive analysis of common diseases and disease occurrence causes of the laboratory monkeys, suggesting that providing better quality laboratory monkeys for scientific research only by constantly improving the scientific breeding management level, constantly strengthening veterinary capacity, mastering the theoretical knowledge and scientific diagnosis method. 相似文献
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85.
免疫抑制引起免疫失败及对病原的易感性增加,是目前乃至今后威胁家禽健康的最危险的潜在因素之一。作者阐述与讨论了几种目前常见的家禽病毒性免疫抑制病的概念、免疫抑制机理及防制措施,以此引起同行对该病的进一步重视。 相似文献
86.
一株无血凝性兔出血症病毒的分离鉴定及其衣壳蛋白VP60基因的克隆和序列分析 总被引:1,自引:0,他引:1
从临床病料中分离鉴定出1株无血凝性的致病性兔出血症病毒(RHDV),命名为Yaan-1。HA试验测得该毒株原代及经兔体传3代后的肝毒在25℃、37℃和4℃时均无血凝性;但在琼脂扩散试验和对流免疫电泳中均可见分离株与常规RHDV高免血清形成清晰的沉淀线;电镜观察可见30nm左右的病毒粒子。采用RT-PCR方法将Yaan-1株的衣壳蛋白VP60基因进行了克隆测序,序列分析表明VP60基因序列全长1740bp,编码579个氨基酸,测序结果提交GenBank(注册号为DQ069280),并与世界各国的常规RHDV分离株进行比较,结果表明核苷酸同源性为90.0%~98.0%,氨基酸同源性为94.1%~99.0%,氨基酸变异多发生在衣壳蛋白C、E区;同时对Yaan-1株的分子量、等电点、疏水性、跨膜区等分子结构特征进行了分析。本研究首次报道了无血凝性RHDV中国分离株,丰富了地方毒株资源,为明确我国地方株的分子流行病学,以及对VP60蛋白分子特征研究提供了新材料。 相似文献
87.
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89.
Nabity MB Boggess MM Kashtan CE Lees GE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(3):425-430
BACKGROUND: Interpretation of serial urine protein:creatinine (UPC) values is confounded by a lack of data regarding random biologic variation of UPC values in dogs with stable glomerular proteinuria. HYPOTHESIS: That there is minimal day-to-day variability in the UPC of dogs with unchanging proteinuria and the number of measurements needed to reliably estimate UPC varies with the magnitude of proteinuria. ANIMALS: Forty-eight heterozygous (carrier) female dogs with X-linked hereditary nephropathy (XLHN) causing stable proteinuria. METHODS: Urine samples were obtained daily by cystocentesis for 3 consecutive days on 183 occasions (549 samples). The UPC was measured for each sample with a single dry-film chemistry auto-analyzer. Data were analyzed retrospectively by a power of the mean model because the variance of UPC values within the 3-day evaluation periods increased as the magnitude of proteinuria increased. RESULTS: To demonstrate a significant difference (P < .05) between serial values in these proteinuric dogs, the UPC must change by at least 35% at high UPC values (near 12) and 80% at low UPC values (near 0.5). One measurement is adequate to reliably estimate the UPC when UPC <4, but 2-5 determinations are necessary at higher UPC values. CONCLUSIONS AND CLINICAL IMPORTANCE: These guidelines for interpretation of serial UPC values in female dogs with XLHN may also be helpful for interpretation of UPC values in dogs with other glomerulopathies. 相似文献
90.
Garner BC Wiedmeyer CE 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(3):240-244
Background: It has been speculated that renal disease can be identified through the detection and quantification of microalbuminuria, however, reliable measurement of albuminuria in any quantity can be challenging. Recently, a new point‐of‐care immunoassay was validated for the specific detection of microalbuminuria and early renal disease in dogs. Objectives: The goal of this study was to determine if measurement of microalbuminuria by the point‐of‐care immunoassay correlated with results from routine semiquantitative methods for detecting proteinuria in dogs. Methods: One hundred and thirty‐eight urine samples, from 133 different dogs, submitted for urinalysis to the Clinical Pathology Laboratory at the University of Missouri‐Columbia Veterinary Medical Teaching Hospital were eligible for the study. Samples that contained >20 RBC/high power field (hpf) or >20 WBC/hpf were excluded, as were samples with insufficient volume to complete all tests. All samples were evaluated with a urinary dipstick with or without a sulfosalicylic acid turbidimetric test, a urine protein:creatinine (UPC) ratio, and the immunoassay for microalbuminuria. Data were analyzed by the Spearman rank order correlation. Results: Microalbuminuria results correlated significantly with those of the dipstick (r= 0.715), sulfosalicylic acid test (r= 0.742), and UPC ratio (r= 0.830). Correlation between the immunoassay and UPC ratio was the same (r= 0.830) when only samples with trace or 1+ proteinuria by dipstick were analyzed (n = 51). Conclusions: The point‐of‐care immunoassay results for microalbuminuria correlated with the results of semiquantitative methods for detecting total proteinuria in dogs. Routine methods for canine proteinuria appear to be adequate for determining whether further testing for renal disease is warranted. 相似文献