农民合作社组建联合社的影响因素分析

农民合作社联合社是农民合作社发展到一定阶段的产物,也是我国农民合作社发展的必然趋势。基于战略联盟和资源依赖理论,利用8省12县614家农民合作社数据,运用二元Probit模型,分析影响农民合作社加入联合社的因素。结果表明,农民合作社加入联合社的比例为14.5%,相比于东部地区,中西部地区农民合作社加入联合社的概率较低。加入联合社受农民合作社的无形资产状况、人力资源和外部环境特征等因素影响,其中无形资产和外部环境对农民合作社加入联合社有显著影响,掌握销售渠道和品牌等资源、被评为示范社和规模小的农民合作社倾向于组建联合社。加入联合社具有示范效应,一个地区农民合作社加入联合社的比例越高,农民合作社选择加入联合社的概率就越大。理事长年龄和受教育程度对农民合作社加入联合社没有显著影响。因此,为了促进联合社健康发展,政府应顺应农民合作社高质量发展趋势,充分运用财政、税收等政策扶持农民合作社发展,加大联合社相关政策宣传力度,不断提升联合社服务能力。     

基于EAHP的农机供应链服务质量评价应用

为提高农机供应链服务水平,针对以制造企业为核心的农机供应链,从组织形态模型、服务业务、价值增值和其他方面四个角度建立农机供应链服务质量评价体系。建立指标数据的可拓物元模型,通过可拓层次分析法,定性定量的表达出农机供应链服务质量的等级及其在该等级范围中的水平,并结合具体案例进行评价。结果表明,该农机供应链的服务质量为优等级,关联度达到0.0727,与供应链实际运作情况一致。     

我国肉羊产业链间的价格传导

为厘清肉羊产业链间价格的传导,本研究根据肉羊产业链上中下游各个环节产品价格的月度数据,构建了向量自回归(VAR)模型,并通过脉冲响应函数与方差分解分析了外部冲击对肉羊产业链价格体系传递的影响。结果表明:只有饲料价格、活羊价格的波动是羊肉价格波动的格兰杰因果;从脉冲响应图来看,羊肉价格对羊肉价格、饲料价格、活羊价格扰动作用的响应分别在当期、第2期和第3期达到最大,随后逐渐减弱;从预测方差的分析结果来看,活羊价格和羊肉价格对羊肉价格变动的贡献率较大。最后从产业链视角完善现有的肉羊价格应急机制;完善肉羊市场流通机制,提高价格传递的效率;推进适度规模化肉羊养殖,促进肉羊产业链纵向联合3个方面提出了政策建议。     

VH1-44 gene usage defines a subset of canine B-cell lymphomas associated with better patient survival

The use of specific immunoglobulin heavy chain variable region (VH) genes has been associated with increased patient survival in human B-cell lymphomas (hBCL). Given the similarity of human and canine BCL (cBCL) in morphology and clinical treatment, we examined the choice of VH in cBCL and determined whether VH gene selection was a distinct feature associated with survival time in dogs. VH gene selection and mutational status in 52 cBCL, including 29 diffuse large B-cell lymphomas (cDLBCL, the most common subtype of cBCL), were analyzed by comparison with the 80 published canine germline VH gene sequences. We further examined the prognostic impact of the subgroups defined by these features on canine survival. We found that VH1-44 was preferentially expressed in the majority of the 52 cBCLs (60%) as well as in the majority of the cDLBCL subset (59%). VH1-44 gene expression was associated with a statistically better overall survival (p = 0.039) in cBCL patients, as well as in the cDLBCL subset of patients (p = 0.038). These findings suggest that VH gene selection in cBCL is not random and may therefore have functional implications for cBCL lymphomagenesis, in addition to being a useful prognostic biomarker.     

Herd diagnosis of low pathogen diarrhoea in growing pigs – a pilot study

Background

The major indication for antibiotic use in Danish pigs is treatment of intestinal diseases post weaning. Clinical decisions on antibiotic batch medication are often based on inspection of diarrhoeic pools on the pen floor. In some of these treated diarrhoea outbreaks, intestinal pathogens can only be demonstrated in a small number of pigs within the treated group (low pathogen diarrhoea). Termination of antibiotic batch medication in herds suffering from such diarrhoea could potentially reduce the consumption of antibiotics in the pig industry. The objective of the present pilot study was to suggest criteria for herd diagnosis of low pathogen diarrhoea in growing pigs.Data previously collected from 20 Danish herds were used to create a case series of clinical diarrhoea outbreaks normally subjected to antibiotic treatment. In the present study, these diarrhoea outbreaks were classified as low pathogen (<15% of the pigs having bacterial intestinal disease) (n =5 outbreaks) or high pathogen (≥15% of the pigs having bacterial intestinal disease) (n =15 outbreaks). Based on the case series, different diagnostic procedures were explored, and criteria for herd diagnosis of low pathogen diarrhoea were suggested. The effect of sampling variation was explored by simulation.

Results

The diagnostic procedure with the highest combined herd-level sensitivity and specificity was qPCR testing of a pooled sample containing 20 randomly selected faecal samples. The criteria for a positive test result (high pathogen diarrhoea outbreak) were an average of 1.5 diarrhoeic faecal pools on the floor of each pen in the room under investigation and a pathogenic bacterial load ≥35,000 per gram in the faecal pool tested by qPCR. The bacterial load was the sum of Lawsonia intracellularis, Brachyspira pilosicoli and Escherichia coli F4 and F18 bacteria per gram faeces. The herd-diagnostic performance was (herd-level) diagnostic sensitivity =0.99, diagnostic specificity =0.80, positive predictive value =0.94 and negative predictive value =0.96.

Conclusions

The pilot study suggests criteria for herd diagnosis of low pathogen diarrhoea in growing pigs. The suggested criteria should now be evaluated, and the effect of terminating antibiotic batch medication in herds identified as suffering from low pathogen diarrhoea should be explored.

Electronic supplementary material

The online version of this article (doi:10.1186/2046-0481-67-24) contains supplementary material, which is available to authorized users.     

鲜切生菜供应链温度波动与微生物含量变化

选取北京某优质农产品种植公司的6月中旬、12月下旬2条具有代表性的鲜切生菜供应链为研究对象,对整个链条温度波动规律分别进行为期7d的实地考察测量;并根据调研的温度曲线,在实验室条件下模拟供应链温度,测定鲜切生菜的微生物含量变化.研究结果显示,2条供应链温度都呈现出较大波动性：6月中旬实际供应链温度波动范围为在1.2～24.1℃,12月下旬为-6.1～16.2℃;运输过程温度波动较小,装车卸货等环节温度波动较明显.实验室模拟条件下得到的鲜切生菜微生物含量变化规律与供应链温度波动曲线存在正相关性,相关系数6月中旬0.849 77,12月下旬为0.847 99.     

水稻OsNHO1基因cDNA序列分离及表达分析

OsNHO1是一种甘油激酶,受多种因素的诱导,在植物先天免疫反应中起着重要的作用。笔者采用同源序列法,根据已报道的拟南芥NHO1基因序列结合水稻基因组测序结果,筛选水稻OsNHO1基因,采用RT-PCR获得水稻OsNHO1基因全长cDNA(1 590 bp,529AA),并通过半定量RT-PCR方法分析OsNHO1基因在SA,PXO99刺激下的表达模式,结果显示OsNHO1基因的表达受SA,PXO99的诱导,为进一步研究OsNHO1的具体功能和作用机理奠定了基础。