KH2PO4对设施油桃果实糖积累及代谢相关酶活性的影响

以设施栽培的艳光油桃为试材,研究了设施栽培条件下,喷施KH2PO4处理对油桃果实中糖积累和蔗糖代谢相关酶活性的影响。结果表明,幼果期喷施KH2PO4处理,果实葡萄糖和果糖含量明显增加,蔗糖含量没有明显变化,成熟期三种糖含量与对照均无明显差异;果实着色前KH2PO4处理显著增加了果实中蔗糖含量,但对葡萄糖和果糖含量无明显影响。幼果期和着色前喷施KH2PO4均显著提高了果实中蔗糖代谢酶活性。说明KH2PO4处理是通过全面提高果实中蔗糖代谢酶活性,起到提高库强作用,从而增加果实含糖量。     

Nitrogen Accumulation and Translocation for Winter Wheat under Different Irrigation Regimes

The translocation of pre‐anthesis nitrogen to the grain is an important source for winter wheat. The relation between the nitrogen translocation and irrigation regime was studied in the field under a rain‐proof trough shelter. Nitrogen (N) translocation amount, N translocation efficiency decreased with a decline in irrigation amount or by excessive irrigation. Compared with different organs, the leaf and stem had higher N translocation amounts, and contributions to grain for both cultivars – Jinan 17 and Lumai 21, indicating that stem also is a major N source for grain development. The contribution of pre‐anthesis total above ground N to grain N ranged from 57 to 76 %, indicating the importance of pre‐anthesis storage of N for achieving high grain N concentrations. Grain nitrogen and yield (kg ha^?1) were positively and significantly correlated with the N translocation amounts and contributions, respectively, suggesting that the sink strength may be involved in the translocation of N from a vegetative organ to the grain. N harvest index (NHI) was significantly correlated with N translocation efficiency, suggesting that the latter is a prerequisite for increasing grain N and improving grain quality. The experiment showed that N translocation status is enhanced by better irrigation practices, but limited by severely deficient or excessive irrigation.     

黄土高原沟壑区人工植被类型对土壤水分和碳氮的影响

以黄土高原沟壑区人工建造的典型植被油松、侧柏、刺槐、沙棘、苹果为研究对象，研究分析了不同植被类型对土壤水分含量、土壤碳氮累积等的变化影响。研究表明，旱季刺槐林地表现出了强烈的耗水特征，200cm以下土壤含水量具明显减少趋势。200—300cm处水分含量仅为5．8％～7．1％，而油松林地200cm以下与荒草地含水量基本相一致，土壤水分含量保持在15％左右，侧柏、沙棘、苹果土壤水分利用主要在20-200cm范围内。雨季，天然降雨虽对林地水分含量有所补充，但只表现在0-50cm表层，且各个植被类型间表现不明显；与荒草相比，油松群落土壤有机碳氮提高了9．3％，果园土壤有机碳含量显著降低，而沙棘、侧柏和刺槐群落的土壤有机碳含量虽低于荒草群落，但尚未达到显著水平。     

化学调控和施磷对玉米/花生间作磷吸收利用和间作优势的影响

为了研究化学调控和磷肥对玉米/花生间作体系磷营养的调控效应,于2012—2013年在河南科技大学农场进行田间试验,试验设单作玉米、单作花生、玉米/花生间作和玉米/花生间作+化学调控4种种植方式,分别施磷[180 kg(P2O5)·hm–2]和不施磷共8个处理,研究了间作玉米和间作花生不同器官磷含量、磷积累分配的特点,分析了化学调控和施磷对间作体系磷间作优势的影响。结果表明:间作显著提高了玉米籽粒磷含量、茎和籽粒磷积累量,促进了磷向籽粒分配;明显降低了花生各器官磷含量、磷积累量,不利于磷向果仁分配;与单作玉米和单作花生相比,间作提高了玉米/花生间作体系磷吸收量,磷吸收间作优势为15.99~19.54 kg(P)·hm–2。在玉米小口期喷施化学调控剂提高了间作玉米籽粒磷含量,降低了茎、叶和籽粒的磷积累量,提高了磷向籽粒分配比例;提高了间作花生果仁磷含量和茎、叶和果仁磷积累量,促进了磷向果仁分配。化学调控间作玉米施磷肥显著增加了间作玉米和花生各器官的磷含量以及磷积累量,提高了磷向玉米籽粒和花生果仁分配比例,促进了间作体系对磷的吸收;磷吸收间作优势为19.50~22.00 kg(P)·hm–2,比不施磷提高16.51%~57.51%,达到显著差异水平。这表明玉米/花生间作具有明显磷间作优势,化学调控间作玉米再施磷有利于间作体系磷吸收量增加,显著提高磷间作优势。因此,生产上可以采用化学调控同时施磷肥来进一步提高磷间作优势。     

基于高光谱的冬油菜植株氮素积累量监测模型

为无损和定量研究高光谱技术在冬油菜植株氮素积累量(PNA,plant nitrogen accumulation)时空变化监测的适宜性及准确性,该文以两年田间氮肥水平试验为基础,采用单变量线性和非线性回归方法,建立基于特征光谱参数的冬油菜P NA高光谱估算模型。结果表明,采用比值光谱的方法可显著提高冬油菜冠层光谱反射率与PNA间的相关性,其最佳的波段组合为1 259 nm与492 nm处光谱反射率比值(R1259/R492),决定系数R2为0.85。高光谱参数间,以比值植被指数(RVI-5)、归一化光谱指数(NDSI)、线性内插法红边位置(REIP)、三角植被指数(TVI)、742 nm处一阶微分光谱值(FD742)和红边面积(SDR)等光谱参数与PNA相关性较好(平均R2和标准误SE分别为0.69和42.70),且以FD742表现最优(R2=0.79,SE=35.66)。精度分析结果显示,以光谱参数R1259/R492和FD742为自变量的指数方程模型作为高光谱监测油菜PNA的最佳模型,各生育期Noise Equivalent(NE)均较低且表现稳定,同时模型估测精度较高,R2分别为0.98和0.98,相对均方根误差RRMSE分别为0.73和0.72,相对误差MRE分别为14.42%和10.31%。该方法为快捷和精确评估冬油菜PNA提供了新的研究思路。     

两种禾本科冬绿肥生长规律及腐解特征比较

在山东临沂研究了冬播绿肥黑麦(冬牧70)和大麦的生长发育、养分吸收规律,并通过原位培养法比较了绿肥翻压后腐解及养分释放的差异。结果表明,冬牧70和大麦在返青后均迅速积累干物质,大麦的快速生长期在3月下旬,冬牧70的快速生长期在4月上旬,冬牧70进入快速生长期后的生长速率高于大麦。两种绿肥的养分吸收规律与干物质积累规律一致,冬牧70和大麦的氮、磷、钾吸收量基本一致。两种绿肥在翻压30 d内快速腐解,腐解率和氮素释放率达到了80%以上,大麦的腐解速率高于冬牧70,而矿质营养元素的释放率低于冬牧70,根据腐解和养分释放规律确定两种禾本科绿肥的适宜翻压时间为4月上旬。     

连续四年施用规模化猪场猪粪温室土壤重金属积累研究

分别采集连续施用0～4年猪粪的蔬菜温室土壤和蔬菜果实样品，测定土壤和果实中Cu、Zn、Pb、Mn、Cr、Cd、Ni和As全量以及土壤中Cu、Zn、Pb、Mn、Cd和As有效态含量，对测定数据进行了Fisher小样本非参数随机化显著水平检验，研究连续4年施用规模化猪场猪粪的温室土壤重金属积累、重金属有效态含量变化及对蔬菜可食部分的影响。结果表明，连续4年施用猪粪，主要导致土壤中Cu和Zn的积累，As有一定积累，Pb、Cd、Ni和Cr短期积累效应不明显，Mn积累不明显，长期大量施用猪粪，Pb、Cd、Ni和Cr有积累的风险；土壤pH值降低；Cu、Zn有效态与全量呈显著正相关(p<0.05)，与pH值呈极显著负相关(p<0.01)；Pb、Cd和Mn有效态与pH值呈显著负相关(p<0.05)；Cu、Zn、Pb、Cd和Mn有效态含量增加；有效态As与全As含量和pH值相关性不明显，但连年施用猪粪As有效态含量增加；连续4年施用规模化猪场猪粪的温室生产的部分番茄和黄瓜As含量超标。若连续以150 m³/(hm²·a)的猪粪施用量于该研究区蔬菜温室，土壤中全Cu和全Zn含量分别经过10年和15年可能超过国家农田土壤二级标准。该研究可为降低温室土壤重金属污染风险提供依据。     

Sampling saproxylic beetles: lessons from a 10-year monitoring study

We sampled saproxylic beetles using trunk window traps in two birch-dominated forests in Finland during 1990-1999. The sampling scheme, 10 traps attached to living fruiting bodies of Fomes fomentarius growing on dead birches, remained unchanged in both forests throughout the study period. Beetles belonging to 32 selected families were identified every year, whereas all species were identified during the last 4 years. Total number of identified individuals was 40,294 and number of species 583, of which 258 were saproxylic (dependent on dead wood). Species richness of rare and threatened saproxylic beetles in the samples varied a lot between the years and did not fluctuate synchronously between the forests. Variation between years was smaller when all saproxylic species were pooled together and some abundant species fluctuated synchronously in the two forests. Similarity indices and DCA-ordination did not generally suggest decreasing similarity between samples with increasing temporal isolation. Incidence-based similarities of common saproxylic species within and between forests and years were high (means 0.7-0.8), whereas those of rare saproxylics were roughly 50% smaller and much more variable. More than 75% of the common saproxylic species found during the entire 10-year period were detected already after 3 years of sampling but accumulation of rare and threatened species was much slower. Our results suggest that: (1) occurrence of rare and threatened species in samples is much less predictable than that of common species and, e.g. reserve selection based on rarities should be made cautiously; (2) estimation of total number of threatened species in a forest is very difficult, because such species accumulate slowly in the samples; (3) samples from different years can be comparable in certain cases.     

‘小偃60’在不同水氮条件下的用水特性

以过筛土壤为培养基础,以圆形桶为容器,设置了3个水分梯度(W0、W1、W2,分别为田间持水量的50%、65%、80%)、4个氮素梯度(N0、N1、N2、N3,施氮量分别为每桶0 g·kg?1、0.10 g·kg?1、0.20 g·kg?1、0.29 g·kg?1),探究冬小麦新品系‘小偃60’干物质积累与分配、水分利用效率、光合参数的变化规律,为其推广及高产高效栽培提供依据。结果表明,水、氮均对冬小麦生物量及产量、叶片叶绿素含量、叶水势、叶片净光合速率等有显著影响,且水对籽粒产量的主效应大于氮肥。水分和氮肥适宜处理(W2N2)比水分和氮肥轻度亏缺处理(W1N1)增产66.03%,W1N1比水分和氮肥严重亏缺(W0N0)增产153.30%。在相同水分处理下,冬小麦叶绿素SPAD值随着施氮量的升高而升高,而在相同施氮量条件下,水分严重亏缺处理(W0)和水分适宜处理(W2)的冬小麦叶绿素SPAD值均低于水分轻度亏缺处理(W1)。水分对叶片水势为正效应,表现为随着土壤含水量的增加显著升高到不明显升高趋势。光合速率(Pn)、蒸腾速率(Tr)、瞬时水分利用效率(WUEi)均随着施氮量的增加而升高,Pn、Tr随土壤水分提高而上升,WUEi却随土壤水分提高而下降。在低氮(N1)或者不施氮肥(N0)条件下,产量水分利用效率(WUEy)随水分的增加表现先升高而后变化不明显的变化趋势;在适宜氮肥(N2)和高氮(N3)条件下,WUEy随着水分的增加而升高。通过对试验结果的分析得出如下结论:1合理的水氮管理使‘小偃60’的籽粒产量及水分利用效率(WUE)维持在较高水平,过多水肥均引起产量及WUE下降;2不同水氮下籽粒产量、水分利用效率均与叶片净光合速率显著正相关;3‘小偃60’在河北环渤海地区更适合在低水中肥条件下种植。     

Intensive tree planting facilitates tropical forest biodiversity and biomass accumulation in Kibale National Park, Uganda

The extensive area of degraded tropical land and the calls to conserve forest biodiversity and sequester carbon to offset climate change demonstrate the need to restore forest in the tropics. Deforested land is sometimes replanted with fast-growing trees; however, the consequences of intensive replanting on biomass accumulation or plant and animal diversity are poorly understood. The purpose of this study was to determine how intensive replanting affected tropical forest regeneration and biomass accumulation over ten years. We studied reforested sites in Kibale National Park, Uganda, that were degraded in the 1970s and replanted with five native tree species in 1995. We identified and measured the size of planted versus naturally regenerating trees, and felled and weighed matched trees outside the park to calculate region-specific allometric equations for above-ground tree biomass. The role of shrubs and grasses in facilitating or hindering the establishment of trees was evaluated by correlating observed estimates of percent cover to tree biomass. We found 39 tree species naturally regenerating in the restored area in addition to the five originally planted species. Biomass was much higher for planted (15,675 kg/ha) than naturally regenerated trees (4560 kg/ha), but naturally regenerating tree regrowth was an important element of the landscape. The establishment of tree seedlings initially appeared to be facilitated by shrubs, primarily Acanthus pubescens and the invasive Lantana camara; however, both are expected to hinder tree recruitment in the long-term. Large and small-seeded tree species were found in the replanted area, indicating that bird and mammal dispersers contributed to natural forest restoration. These results demonstrate that intensive replanting can accelerate the natural accumulation of biomass and biodiversity and facilitate the restoration of tropical forest communities. However, the long-term financial costs and ecological benefits of planting and maintaining reforested areas need to be weighed against other potential restoration strategies.