全文获取类型
收费全文 | 2505篇 |
免费 | 133篇 |
国内免费 | 208篇 |
专业分类
林业 | 127篇 |
农学 | 208篇 |
基础科学 | 38篇 |
142篇 | |
综合类 | 1114篇 |
农作物 | 86篇 |
水产渔业 | 124篇 |
畜牧兽医 | 701篇 |
园艺 | 117篇 |
植物保护 | 189篇 |
出版年
2024年 | 11篇 |
2023年 | 28篇 |
2022年 | 44篇 |
2021年 | 74篇 |
2020年 | 77篇 |
2019年 | 81篇 |
2018年 | 53篇 |
2017年 | 78篇 |
2016年 | 121篇 |
2015年 | 90篇 |
2014年 | 121篇 |
2013年 | 123篇 |
2012年 | 176篇 |
2011年 | 189篇 |
2010年 | 172篇 |
2009年 | 139篇 |
2008年 | 125篇 |
2007年 | 155篇 |
2006年 | 125篇 |
2005年 | 120篇 |
2004年 | 92篇 |
2003年 | 85篇 |
2002年 | 62篇 |
2001年 | 79篇 |
2000年 | 60篇 |
1999年 | 48篇 |
1998年 | 47篇 |
1997年 | 53篇 |
1996年 | 35篇 |
1995年 | 48篇 |
1994年 | 24篇 |
1993年 | 14篇 |
1992年 | 19篇 |
1991年 | 16篇 |
1990年 | 15篇 |
1989年 | 17篇 |
1988年 | 11篇 |
1987年 | 5篇 |
1986年 | 4篇 |
1985年 | 2篇 |
1983年 | 2篇 |
1981年 | 3篇 |
1980年 | 1篇 |
1956年 | 2篇 |
排序方式: 共有2846条查询结果,搜索用时 46 毫秒
51.
52.
以ANAE染色法和~3H-TdR掺入的淋巴细胞转化试验,测定了伊氏锥虫弱毒株免疫家兔的T细胞动态变化.结果,免疫兔外周血T细胞增殖反应在免疫后第24d达到高峰,ANAE阳性细胞百分率这72.65%,对照组仅为41.2%;约1周后,出现抑制性T细胞,其增殖反应也明显减弱,最后完全被抑制.而外源性白细胞介素2的加入,可解除这种免疫抑制.攻虫后,特异性T细胞应答反应未再出现强烈的回忆反应,但伊氏锥虫弱毒株免疫家兔获得9/12的保护.因此,T细胞在这种抗锥虫免疫中,没有起到主导作用. 相似文献
53.
聚类分析在家畜引种中的应用 总被引:3,自引:0,他引:3
本文以小尾寒羊为例,应用聚类分析的方法对三十一个生态区域的八个气候因子进行了分类研究,介绍了聚类分析在家畜引种中的应用。结果表明,这三十一个生态区域可以分为六类,凡与小尾寒羊原产地分为同一类的生态区域可以引种小尾寒羊,其他类的生态区域不宜引种小尾寒羊。 相似文献
54.
猴丰1号新菌株的选育及其酯酶同工酶分析 总被引:2,自引:0,他引:2
采用子实体组织分离与系统筛选法,从福建猴头的变异株中选育出了定名为“猴丰1号”的新菌株。该菌株菌丝体生长势强,发菌迅速;子实休菇形大,产量高。鲜菇产量比出发株福建猴头增加69.3%,比著名的常山99菌株增加16.8%。酯酶同工酶分析显示:猴丰1号的酯酶谱型与福建猴头相似,但有1条酶带发生了变异。由此说明,它确是源于福建猴头的自然变异株。 相似文献
55.
An isolate of Newcastle disease virus obtained from a guinea fowl was characterized as a viscerotropic velogenic strain based upon pathogenicity index studies. Following inoculation of the viral isolate oronasally into 3-week-old chickens, clinical signs appeared after an incubation period of 4–5 days and included dullness, depression, dyspnoea, diarrhoea and leg paralysis. The virus caused a mortality of 56% with haemorrhages at the tip of the glands of the proventriculus and caecal tonsil. Histopathological changes were prominent in the lymphoid organs, being characterized by depletion, degeneration and necrosis of the lymphoid tissues. The brain was the first organ affected, with changes being noticed 3 days after infection. Isolation of virus from various organs was more frequent from 5 to 10 days after infection, but the virus persisted in some of the organs until 21 days after infection. In spite of the high mortality, a good immune response was elicited by the isolate, as was evident from the antibody titre. 相似文献
56.
Glycoprotein E-negative (gE–) laboratory strains of bovine herpesvirus 1 (BHV-1) were recently introduced as novel marker vaccines, allowing serological discrimination between vaccinated and naturally infected animals on the basis of lack or presence of antibodies against gE epitopes. The applicability of this approach is based on the genetic stability of the gE. However, mutant field variants of BHV-1 with a variable response in anti-gE ELISA have been isolated. The molecular characterization of a gE variant field isolate (Salwa strain) is presented here. By comparing the gE nucleotide and amino acid sequences of the Salwa strain with those of the wild strain Jura, ten mutated bases were found in the gE strain of Salwa, six of which alter the amino acid sequence, leading to changes in five amino acids. Both strains caused respiratory disease in experimentally infected calves, but Salwa generated slightly milder signs. Both viruses were excreted in nasal and ocular discharges, and were reactivated by dexamethasone treatment. In conclusion, the rather close similarities observed in the gE gene structure and pathogenicity features of the gE mutant and of the wild strain of BHV-1 confirm the genetic stability of gE. The findings indicate that the Salwa isolate is virulent, but less virulent than wild strains. Our data support the use of gE-negative marker vaccines in eradication programmes. 相似文献
57.
不同饲料蛋白质水平对吉富品系尼罗罗非鱼幼鱼生长和鱼体组成的影响 总被引:11,自引:0,他引:11
用3种不同蛋白含量(23.1%、37.6%和47.9%)的配合饲料投喂吉富品系尼罗罗非鱼幼鱼78d,结果显示,①随着饲料蛋白质含量从23.1%上升到47.9%,平均日增重从0.30g/d上升到0.74g/d,3组之间的日增重和体重有显著差异(P<0.05);饲料系数从1.53下降为0.98,3组之间的饲料系数有显著差异(P<0.05)。②不同的蛋白质水平对鱼体蛋白质和脂肪的含量有影响,各组之间差异显著(P<0.05),对灰分的影响差异不显著(P>0.05)。③蛋白含量为23.1%、37.6%和47.9%的配合饲料的成本分别为2.77元/kg、3.67元/kg、4.60元/kg。④初步认为蛋白含量为37.6%的配合饲料更适合吉富品系尼罗罗非鱼苗种培育的生产需要。 相似文献
58.
以BALB/c小鼠为实验动物模型,对actA基因和plcB基因双缺失的产单核细胞李氏杆菌(LM)yzuLM1-2菌株进行感染动力学研究。分别用减毒突变株yzuLM1-2和野生型菌株yzuLM4给BALB/c小鼠静脉注射或灌服,并对脏器中LM的分布动态进行测定。结果显示,在显著高于野生型菌株感染剂量的条件下注射或灌服接种后,减毒突变株组小鼠肝和脾中的LM数量迅速下降,被清除的速度显著快于野生型菌株组。溶血素(LLO)抗体的测定结果显示,减毒突变株菌能和野生型菌同样激发较高水平的抗体。小鼠免疫保护试验结果显示,减毒突变株免疫组对同种血清型LM的攻击具有较好的免疫保护力,可达100%。结果表明,yzuLM1-2突变株侵袭力下降,致病力降低,能激发较高水平的抗体产生,可以作为预防动物李氏杆菌病的候选疫苗菌株,并为用作运送外源保护性抗原的载体提供了材料。 相似文献
59.
60.
Reclassification of an isolate of Guignardia citricarpa from New Zealand as Guignardia mangiferae by sequence analysis 总被引:3,自引:0,他引:3
Citrus black spot was recorded as present in New Zealand in international databases on the basis of one isolate (ICMP 8336) identified by morphological features as Guignardia citricarpa . This isolate was from a soft rot, not a typical symptom of citrus black spot, on a Seminole tangerine fruit grown in the northern region of New Zealand. Sequence analysis of the internal transcribed spacer (ITS) region (ITS1, 5·8S and ITS2) showed that this isolate was 99% identical to the ITS region of G. mangiferae , a closely related saprotroph. Despite climatic conditions being suitable for this disease, citrus black spot symptoms have never been seen on citrus fruits grown in New Zealand. Thus the absence of symptoms on citrus in New Zealand is probably because G. citricarpa is not present. On the basis of these results, the record of citrus black spot occurring in New Zealand should be re-examined. 相似文献