全文获取类型
收费全文 | 956篇 |
免费 | 62篇 |
国内免费 | 128篇 |
专业分类
林业 | 30篇 |
农学 | 65篇 |
基础科学 | 210篇 |
151篇 | |
综合类 | 295篇 |
农作物 | 31篇 |
水产渔业 | 143篇 |
畜牧兽医 | 68篇 |
园艺 | 49篇 |
植物保护 | 104篇 |
出版年
2024年 | 7篇 |
2023年 | 31篇 |
2022年 | 37篇 |
2021年 | 40篇 |
2020年 | 38篇 |
2019年 | 53篇 |
2018年 | 32篇 |
2017年 | 48篇 |
2016年 | 58篇 |
2015年 | 40篇 |
2014年 | 47篇 |
2013年 | 60篇 |
2012年 | 87篇 |
2011年 | 69篇 |
2010年 | 62篇 |
2009年 | 48篇 |
2008年 | 44篇 |
2007年 | 74篇 |
2006年 | 52篇 |
2005年 | 30篇 |
2004年 | 33篇 |
2003年 | 27篇 |
2002年 | 28篇 |
2001年 | 22篇 |
2000年 | 11篇 |
1999年 | 16篇 |
1998年 | 5篇 |
1997年 | 9篇 |
1996年 | 5篇 |
1995年 | 8篇 |
1994年 | 5篇 |
1993年 | 4篇 |
1992年 | 4篇 |
1991年 | 4篇 |
1990年 | 5篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
排序方式: 共有1146条查询结果,搜索用时 15 毫秒
31.
32.
以水稻红莲型细胞质雄性不育(HL-CMS)不育系粤泰A(YTA)和保持系粤泰B(YTB)黄化苗线粒体为材料,研究了YTA和YTB离体线粒体KA TP通道对其诱导调节剂KCl、ATP、ADP和GTP等的响应特性。结果表明,KA TP通道诱导剂KCl,能明显诱导YTA和YTB线粒体膨胀,但YTA的膨胀程度较YTB的明显;KA TP通道的内源性抑制剂ATP对YTA和YTB线粒体膨胀起显著抑制作用;KA TP通道的内源性激动剂ADP和GTP引起的不育系YTA离体线粒体短暂收缩及之后的再膨胀程度均较YTB的明显;此外,比较YTA和YTB离体线粒体在Rh123一起孵育10 min后,KCl和解偶联剂FCCP处理引起的离体线粒体膜电位(Δψm)下降过程,发现前者的线粒体Δψm较后者的易于过早崩解。对水稻HL-CMS不育系YTA和可育的保持系YTB线粒体KA TP通道特性的比较研究表明,水稻HL-CMS不育系YTA线粒体KA TP通道对其诱导调节剂KCl、ATP、ADP和GTP等的响应较YTB的更敏感。 相似文献
33.
Xiaoliang Li Yiliang Lu Liming Li Xinyu Chai Qiushi Ren 《Veterinary ophthalmology》2010,13(2):131-135
Multi‐channel visual evoked potential (MVEP) recording method was used to assist in diagnosing a 4‐month‐old Chinese Albino rabbit with an intraorbital mass. Subcutaneous MVEP of its both eyes were recorded simultaneously using 16 electrodes (4 × 4) multi‐channel array. Analysis of the cortical potential landscapes (CPL) showed that the conduction function of right eye was remarkably impaired in terms of decreased amplitudes and prolonged latencies. Specific side‐dominant distribution asymmetry of the decreased MVEP amplitudes indicated that the temporal side of the optic nerve (ON) was severely involved. Overall prolonged latencies of the CPL without side differences suggested that the functional impairment could have been caused by the mechanical compression exerted by an intraorbital mass. Surgical removal procedures confirmed that the mass was located temporally to the ON. Pathological examination provided a final diagnosis of a giant polycystic mucocele. Beyond its significance as a standard tool to assess functional changes of the visual pathway, MVEP recordings might assist locating intraorbital lesions that involve the ON by careful analysis of abnormal CPLs. 相似文献
34.
The effects of the oxadiazine insecticide indoxacarb and its N-decarbomethoxylated metabolite (DCJW) on tetrodotoxin-resistant (TTX-R) voltage-gated sodium channels in rat dorsal ganglion neurons were studied using the whole-cell patch clamp technique. Indoxacarb and DCJW suppressed the peak amplitude of action potentials, and DCJW exhibited a faster time course and higher potency than indoxacarb in the blocking effects. In voltage-clamp experiments, indoxacarb and DCJW suppressed TTX-R sodium currents in a time-dependent manner without a steady-state level of suppression. IC50 values for indoxacarb and DCJW on TTX-R sodium currents were estimated to be 10.7 and 0.8 microM after 25 min of bath application, respectively. DCJW was about 10 times more potent than indoxacarb in blocking TTX-R sodium currents. Although the suppressive effects of indoxacarb were partially reversible after washout with drug-free external solution, no recovery of sodium current was observed in DCJW treated neurons after prolonged washout. In current-voltage relationships, both indoxacarb and DCJW blocked the sodium currents to the same degree in the entire range of membrane potentials. The sodium conductance-voltage curve was not shifted along the voltage axis by indoxacarb and DCJW at 10 microM. In contrast, the steady-state inactivation curves were shifted in the hyperpolarizing direction by indoxacarb as well as by DCJW. Based on these results, it was concluded that indoxacarb and DCJW potently blocked the TTX-R sodium channel in rat DRG neurons with hyperpolarizing shifts of the steady-state inactivation curves, suggesting preferential association of the insecticides to the inactivated state of sodium channels. The small structural variation between indoxacarb and DCJW resulted in clear differences in potency for blocking sodium channels and reversibility after washout. 相似文献
35.
从发生在四川、重庆等省市的斑点叉尾妇急性流行性传染病病鱼的肝脏、肾脏内分离到1株高致病性菌株(CCF00024),人工感染健康鱼表现出与自然痛鱼相同的症状,并从中分离获得同种细菌,证实其为斑点叉尾鲴急性流行性传染病的病原菌。形态、生理生化检测表明,该菌为非发酵型直杆菌,严格需氧,革兰氏阴性,极生多鞭毛,对除麦芽糖和甘露糖以外的多种糖类不利用产酸,氧化酶阴性,DNA酶、蛋白酶、脲酶、赖氨酸脱羧酶阳性,MR、VP阴性。在以该菌16SrDNA序列(GenBank登录号AY970826)和GenBank及RDP数据库内同源性较高的细菌16SrDNA序列构建的系统发育树中,分离菌CCF00024与嗜麦芽寡养单胞菌(Stenotrophomonasmaltophilia)聚在一簇,特剐是与5.maltophiliaM5—1的同源性最高,序列相似性达99.6%,结合形态和生理生化特点将其鉴定为嗜麦芽寡养单胞菌(Stenotrophomonasmaltophilia)。药敏试验结果表明,对磺胺甲口恶唑、磺胺异口恶唑、阿齐霉素、洛美沙星高度敏感,而对新霉素、卡那霉素、氨苄青霉素、头孢唑啉、先锋霉素V和链霉素不敏感。 相似文献
36.
通过“西繁东育”项目的实施,大通县牛羊贩运育肥规模户由2001年的898户发展到2005年的4170户,贩运育肥出栏牛羊由2001年的12.12万头只增加到2005年的65万头只,纯收入由2001年的456.76万元增长到2005年的4548.97万元,逐步成为大通农民增收的主渠道。 相似文献
37.
- 1. This paper documents a diverse, reproducing freshwater mussel community (20 species) in Lower Lake — an impounded, regulated portion of the Little Tallahatchie River below Sardis Dam in Panola Co., Mississippi, USA.
- 2. Despite being regulated and impounded, the lake has a heterogeneous array of habitats that differ markedly in mussel community attributes. Four distinct habitat types were identified based on current velocity and substrate characteristics, representing a gradient from habitats having lotic characteristics to lentic habitats. All four habitat types supported mussels, but habitats most resembling unimpounded, lotic situations (relatively higher current velocity and coarser substrate) had the highest mussel abundance and species density (10.1 mussels m?2, 1.8 species m?2, respectively). Lentic habitats (no flow, fine substrate) were characterized by lower abundance and species density (2.0 mussels m?2, 0.8 species m?2, respectively), but supported mussel assemblages distinctive from lotic habitats.
- 3. Evidence of strong recent recruitment was observed for most species in the lake and was observed in all four habitat types.
- 4. Although impounded and regulated, Lower Lake represents one of the few areas of stable large‐stream habitat in the region. The presence of a diverse, healthy mussel community in this highly modified habitat suggests that a large component of the regional mussel fauna is relatively resilient and adaptable and is limited primarily by the absence of stable river reaches. Management actions that increase stream stability are likely to result in expansion of the mussel fauna and restoration of a valuable component of ecosystem function in this region.
38.
39.
河流故道区梨树-药材立体种植技术研究及分析 总被引:1,自引:1,他引:0
河流故道区梨园内间作不同药材,研究立体模式种植技术及环境效益。结果表明:梨树-半夏复合系统中,间作半夏不会造成梨减产,半夏株行距3 cm×15 cm时,产量最高563.2kg/hm^2、收获指数最高4.6、商品指数最高57%。梨树-防风复合系统中,行距为40 cm时,防风产量最高21 610.8 kg/hm^2,收获指数最高9.3、商品指数最高100%。梨树-紫菀复合系统中,提出了氮、磷、钾肥料合理配比的施用量为1 hm^2中N∶P2O5∶K2O为115.8∶292.5∶303.5,紫菀产量为16 049.69 kg/hm^2,紫菀铜含量各处理间无显著差异。对3种复合系统进行土地利用价值分析。结果表明:梨树-半夏的LER为1.8最大,梨树-紫菀的LER为1.4最低。 相似文献
40.