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91.
新疆野蔷薇果的研究 总被引:7,自引:0,他引:7
丛者福 《干旱区资源与环境》1996,10(4):100-102
新疆野蔷薇(Rosaspp)果实富含多种人体所需的营养物质,有较大开发利用价值。欲培育大果,多刺蔷薇、刺蔷薇较有前途;疏花蔷薇、腺毛蔷薇、伊犁蔷薇还原型VC含量较高;还可根据需要选择优良个体或类型以培育新品种。 相似文献
92.
马蹄金与匍茎翦股颖光合生理生态的比较研究 总被引:14,自引:1,他引:13
研究表明,1.通过光饱和点、光补偿点、CO_2补偿点及叶绿素a/b比值等测定,马蹄金与匍茎剪股颖均属喜光、耐荫的C_3植物。马蹄金对强光适应性大于匍茎翦股颖。因而在芜湖地区作为大田草坪种植,则马蹄金优于匍茎葛股颖。2.NaHSO_3均有增强光合速率、加速光合产物输出、增加于物质积累及提高生物产量的效应。光呼吸抑制剂在草坪生产上大有可为。 相似文献
93.
94.
de Laforcade AM Freeman LM Shaw SP Brooks MB Rozanski EA Rush JE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(5):674-679
Sepsis is a frequent source of morbidity and mortality in critically ill patients. The goal of this case control study was to measure hemostatic changes in dogs with naturally occurring sepsis. Blood was collected within 24 hours of admission from 20 dogs that fulfilled the criteria for sepsis. Sepsis was defined as histologic or microbiological confirmation of infection and 2 or more of the following criteria: hypo- or hyperthermia, tachycardia, tachypnea, or leukopenia, leukocytosis, or > 3% bands. Culture and sensitivities were performed on appropriate samples from all septic dogs. Twenty-eight control dogs were enrolled on the basis of normal results of physical examination, CBC, serum biochemistry, and coagulation profile. Plasma samples were analyzed for prothrombin time (PT), partial thromboplastin time (PTT), fibrin(ogen) degradation products (FDP), D-dimer (DD) concentrations, antithrombin (AT) activity, and protein C (PC) activity. Data were compared between groups by chi-square or independent t-tests. PC (P < .001) and AT (P < .001) activities were significantly lower in dogs with sepsis compared to controls. Dogs with sepsis had significantly higher PT (P = .007), PTT (P = .005), D-dimer (P = .005), and FDP (P = .001) compared to controls. Platelet counts were not significantly different between groups. Ten of the 20 septic dogs (50%) died, but no association was identified between any of the measured variables and outcome. These findings are consistent with previous studies in animals with experimentally induced disease and in clinical studies of humans. On the basis of these results, further investigation of the role of AT and PC in canine sepsis is warranted. 相似文献
95.
Gatongi PM Njoroge JM Scott ME Ranjan S Gathuma JM Munyua WK Cheruiyot H Prichard RK 《Veterinary parasitology》2003,110(3-4):235-240
Susceptibility to IVM (IVM) of “strain A” Haemonchus contortus which had been exposed to IVM four times over a 2-year period was compared to IVM susceptibility of “strain C” H. contortus which had no prior field exposure to IVM, by in vivo and in vitro methods. In vivo, the percentage reduction in faecal egg counts (FEC) and the total worm counts (TWC) were compared between control animals (lambs and kids) and animals treated with low dose IVM (20 μg/kg). In vitro susceptibility to IVM was evaluated by larval migration inhibition (LMI) after the two strains of H. contortus were exposed to different concentrations of IVM. The dose response, measured as the proportion of larvae inhibited from migrating, was used to estimate LD50. Although differences in response to IVM in the in vivo determinations were not significant, “strain A” H. contortus had a significantly higher LD50 than “strain C” in the LMI assay. Coincident with the conduct of the in vivo experiment, it was observed that “strain A” H. contortus established and survived better than “strain C” in the control lambs. 相似文献
96.
A 3 × 2 factorial design with growing dairy-breed bulls was used to study the effects on animal performance of (1) proportion of concentrate (rolled barley) in the diet, and (2) inclusion of rapeseed meal (RSM) in the barley-based concentrate in a total mixed ration (TMR). The interactions between concentrate proportion and RSM supplement were also examined.Three feeding experiments comprised in total of 84 Finnish Ayrshire bulls and 6 Friesian bulls. The bulls were fed TMR ad libitum. The three concentrate proportions were 300 (L), 500 (M) and 700 (H) g/kg dry matter (DM), fed without RSM (RSM−) or with RSM (RSM+). Rapeseed meal was given so that the crude protein (CP) content of the concentrate was raised to 160 g/kg DM in the RSM+ diets. In the RSM− diets the CP content of the concentrate was 128 g/kg DM, so the CP content increased 25% with RSM supplementation. Increasing the proportion of concentrate led to a linear improvement in daily live weight gain (LWG) (P < 0.05), but there were no significant treatment differences in the DM intake (kg/d). Increasing the proportion of concentrate also led to significantly higher CP (P < 0.001) and phosphorus (P) (P < 0.001) supply and significantly improved DM and organic matter (OM) digestibility (P < 0.001). However, the digestibility of neutral detergent fibre (NDF) decreased (P < 0.001) as the proportion of concentrate increased. The feed conversion rate (kg DM/kg LWG) decreased significantly with increasing concentrate proportion (P < 0.001). Rapeseed meal supplement had no effect on animal performance, but the supply of CP (P < 0.01) and P (P < 0.001) was higher when RSM was included in the diet. The CP (P < 0.001) and NDF (P < 0.05) digestibilities were also higher for the RSM+ diets than for the RSM− diets. Because RSM at the concentration used did not affect animal performance, there is no reason to use RSM supplementation for finishing dairy bulls when there is good quality grass silage and barley-based concentrate in the TMR ration. This study also shows that there is a need to update the Finnish feeding recommendations for dairy-breed growing bulls, and extra calculations are needed for the energy and protein supply of growing dairy bulls. 相似文献
97.
98.
K. Elena 《European journal of plant pathology / European Foundation for Plant Pathology》2006,115(2):259-262
During the last three years, a new disease was observed in northwestern Greece on Minneola trees, hybrid of mandarin and grapefruit. On May small brown necrotic leaf spots surrounded by yellow halo areas of various sizes appeared and covered a major portion of the leaves with extension of necrosis into the veins. On young fruits small, slightly depressed black spots were the first symptoms, which later became 2–7 mm in diameter. Brown spots were observed on the leaves and fruits in several orchards in the same area, causing leaves and fruits to drop. In some orchards over 50% of the fruits were affected. From the fruit and leaf spots the typical small-spore species Alternaria alternata was isolated. Pathogenicity tests were performed by artificially inoculating fruits of Minneola, common mandarin and Clementine. The symptoms of the disease were reproduced only on fruits of Minneola hybrids by the specific strain of the fungus Alternaria alternata pv. citri. Different citrus susceptibility tests indicated that mandarins Minneola, Nova and Page were very susceptible to tested isolates while Clementine SRA and Poros Clementine were not. All lemons and lime Seedless were not susceptible. Grapefruit New Hall was not susceptible, while the Star Ruby was. Orange Lane Late, Navel Late, Oval Poros, Olinda, Navel Athos were not susceptible and only Moro showed reaction being slightly susceptible only to one isolate. 相似文献
99.
Protoporphyrinogen oxidase (Protox) of Myxococcus xanthus (Mx Protox) is a 49-kDa membrane protein that catalyzes conversion of protoporphyrinogen IX (Protogen IX) into protoporphyrin IX (Proto IX). Upon heterologous expression in transgenic rice plants, Mx Protox is dually targeted into plastids and mitochondria, increasing resistance against the herbicidal Protox inhibitor oxyfluorfen. Here, we describe the chemical synthesis of the Mx Protox gene by assembling several small synthetic DNA fragments derived by ligation-PCR. Codon usage in the resulting 1416-bp gene was modified to correspond to that of the Arabidopsis Protox gene, a change that resulted in a decrease in G+C content from 71 to 49%. The modified Mx Protox gene was used to generate transgenic rice plants via Agrobacterium-mediated transformation. Integration, expression, and inheritance of the transgenes were demonstrated by Southern, Northern, and Western blot analyses. In plants transformed with the modified, low G+C-content Mx Protox gene, levels of Protox expression and enzyme activity were low compared to the levels observed for plants transformed with the native Mx Protox gene. Nonetheless, like the native gene, the modified gene conferred a high level of resistance to the herbicide oxyfluorfen in a seedling growth test. 相似文献
100.
LI Wenjun HUANG Huilan YANG Huihu XIE Zimin CUI Huiyi HUANG Shujian ZHANG Xuelian 《中国畜牧兽医》2007,47(9):2953-2959
The aims of the experiment was to optimize the prokaryotic expression system of σC protein,prepare polyclonal antibody against σC protein of novel duck reovirus (NDRV),and evaluate the titer of the antibody.The σC gene of NDRV-DH13 strain was amplified by RT-PCR,ligated into pET-30a(+) and pET-32a(+) expression vector,constructed prokaryotic expression plasmid,which were transformed into E.coli BL21(DE3) and the expression of the σC protein were induced by IPTG.The proteins expression were analyzed by SDS-PAGE.The recombinant protein without His tag was purified by digestion,and the recombinant protein with His-tagged was purified by Ni-NTA column.Then the polyclonal antibody was obtained from rabbits which had been immunized by the purified protein without His tag.Anti-His-labeled mAb and NDRV-σC positive serum were used as primary antibodies to evaluate antibodies specificity,the antibodies titer was detected by indirect ELISA (iELISA).SDS-PAGE results showed that the molecular weight of the expression on recombinant proteins were 34 and 37 ku respectively,the proteins were highly expression.Western blotting showed that they had the specific reaction and the prepared antibodies had higher affinity with σC protein,the titer were about 1:25 600 by iELISA detection.This study successfully constructed and optimized the prokaryotic expression system of the polyclonal antibody against σC protein,laid a foundation for the further study of σC protein and the research of genetically engineered vaccine. 相似文献