全文获取类型
收费全文 | 17807篇 |
免费 | 827篇 |
国内免费 | 1983篇 |
专业分类
林业 | 993篇 |
农学 | 1657篇 |
基础科学 | 740篇 |
2169篇 | |
综合类 | 7411篇 |
农作物 | 1208篇 |
水产渔业 | 769篇 |
畜牧兽医 | 3261篇 |
园艺 | 1405篇 |
植物保护 | 1004篇 |
出版年
2024年 | 47篇 |
2023年 | 215篇 |
2022年 | 421篇 |
2021年 | 569篇 |
2020年 | 597篇 |
2019年 | 756篇 |
2018年 | 620篇 |
2017年 | 798篇 |
2016年 | 952篇 |
2015年 | 796篇 |
2014年 | 901篇 |
2013年 | 1044篇 |
2012年 | 1197篇 |
2011年 | 1230篇 |
2010年 | 1049篇 |
2009年 | 1016篇 |
2008年 | 944篇 |
2007年 | 942篇 |
2006年 | 819篇 |
2005年 | 574篇 |
2004年 | 515篇 |
2003年 | 423篇 |
2002年 | 363篇 |
2001年 | 319篇 |
2000年 | 327篇 |
1999年 | 294篇 |
1998年 | 244篇 |
1997年 | 310篇 |
1996年 | 306篇 |
1995年 | 261篇 |
1994年 | 270篇 |
1993年 | 279篇 |
1992年 | 322篇 |
1991年 | 301篇 |
1990年 | 259篇 |
1989年 | 115篇 |
1988年 | 66篇 |
1987年 | 37篇 |
1986年 | 21篇 |
1985年 | 14篇 |
1984年 | 13篇 |
1983年 | 4篇 |
1982年 | 8篇 |
1981年 | 11篇 |
1980年 | 10篇 |
1979年 | 9篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1956年 | 10篇 |
1955年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
91.
92.
简怀玉 《湖南农业大学学报(自然科学版)》1990,17(3)
本文研究一类2k阶非线性偏微分方程组之解的正则性,没有假定通常的椭圆性条件而只假定所谓"无穷远处"的椭圆性条件,证明了解的k-1阶导数为李普希兹连续的. 相似文献
93.
Although the molluscicide Frescon is a strong neurotoxin to the Lymnaea stagnalis central nervous system in vitro, it is probable that the exposure of the whole animal to this molluscicide fails to result in central nervous system abnormalities: Frescon does not appear to reach the brain in sufficient quantity to disrupt its normal activity. However, only those Frescon analogs found to be neurotoxic were molluscicidal, suggesting some related mode, if not site, of action. Frescon and its analogs may act by affecting excitable tissues other than the nervous system (e.g., the snail musculature) by altering certain functional and/or structural membrane properties. 相似文献
94.
The effects of root-applied chalcone at 0.15 mmol L−1 on the growth and lignin biosynthesis in maize were investigated. The contents of 4-coumarate: CoA ligase (4CL, EC 6.2.1.12) substrates in maize shoots were increased more rapidly in the samples with chalcone application than in the control and the increase occurred at ≤ 3 h after the application (HAA). The lignin content was reduced by chalcone at ≤ 6 HAA. The shoot growth was suppressed by chalcone at ≤ 9 HAA. Consequently, the results suggest that chalcone suppressed maize growth by inhibiting monolignol biosynthesis. 相似文献
95.
96.
97.
中国风水侵蚀交错区分布特征分析 总被引:6,自引:1,他引:6
风水侵蚀交错带的研究 ,对景观、土地覆盖 /土地利用、全球变化等研究具有重要意义。论文以GIS技术为支撑 ,定量计算出我国风水侵蚀带。在分布特征上 ,主要分布我国北方地区 ,其中以西部沙漠 ,北部山脉沿线为重 ,这种分布与气候、地貌分界有明显联系。 49.6 %风水侵蚀复合带的降水量小于 2 0 0mm。风水侵蚀复合带的土地利用主要以草地为主 ,占 41.8% ,其次为耕地。两种类型的分布 ,草地以与其它土地利用类型交错分布为主 ;而耕地是有重心的分散分布。风水侵蚀复合带的总体侵蚀要强于全国水平。 相似文献
98.
AIM:To manufacture recombinant protein of the highly conserved domain in human bone morphogenetic protein-1(BMP-1) using gene engineering methods as antigen for making wide spectrum antibody to BMP-1.METHODS:We analyzed the gene sequences and protein structures of BMP-1 and its related proteins, and chose a highly conserved fragment as target gene. Total RNA was prepared from human osteosarcoma cell line Saos-2, then the target gene was amplified with RT-PCR. The PCR product was cloned into prokaryotic expression vector pMAL c2 to get recombinant vector BMP-1(322-588aa)-pMAL c2. After transforming the recombinant plasmid into DH5-alpha and screening, several prositive clones were got for sequencing. Finally the transformed cells was induced with IPTG to get fusion protein.RESULTS:The BMP-1 gene fragment was successfully cloned into vector pMAL c2, and was able to express efficiently with IPTG inducement. The amount of expressed fusion protein is about 66%-72% in total volume of bacterial proteins.CONCLUSIONS:The recombinant protein contains several key domains(2 CUB domains and 1 EGF domain), which are shared by BMP-1 and its related proteins. Specific wide spectrum antibody to human BMP-1 and its related proteins may be generated with this recombinant protein antigen. 相似文献
99.
ZHOU Yan-bin YE Ren-gao XIE Can-mao XU Han-shi GUAN Wei-ming YANG Xiao YANG Nian-sheng 《园艺学报》2003,19(9):1221-1223
AIM:To investigate the role of CD134 (OX40) and NF-κB in the pathogenesis of lupus nephritis (LN).METHODS:Renal in situ CD134 and NF-κB expression were examined in 40 biopsy specimens from LN patients by immunohistochemistry and microwave-based immunohistochemistry, respectively. The relationship between expression of CD134 and NF-κB was analyzed.RESULTS:The expression of glomerular and tubular CD134 and NF-κB in LN were higher than that in normal control, especially in class Ⅳ LN, where there was intense staining of endothelial cell, distal tubules, and interstitial mononuclear cell. The CD134 expression of glomerular and tubular was closely related to NF-κB expression, respectively (r=0.5542, P<0.05;r=0.6279, P<0.05). CONCLUSIONS:The abnormal expression of costimulatory molecule CD134 was well evidenced in LN. Strong expression of renal in situ NF-κB was likely mediated by CD134 signal pathway, which may play an important role in the pathogenesis of LN. 相似文献
100.
AIM:To investigate the expression of Th1-typed cytokine IFN-γ and Th2-typed cytokine IL-4 on T lymphocytes that infiltrate in nasal polyps for searching the pathogenesis of nasal polyps. METHODS:Nasal polyps tissue samples and peripheral blood were obtained from 21 patients. Normal human inferior turbinate mucosa and peripheral blood were obtained as well. Flow cytometry was adopted to detect the expression of IFN-γ and IL-4 of T lymphocytes. RESULTS: Th cytokines were rarely detected in inferior turbinate from normal human. Nasal polyps tissue consisted of abundant T lymphocytes. The expression of IL-4 and IFN-γ increased in peripheral blood from patients compared with normal human (P<0.05). The expression of IL-4 increased but the expression of IFN-γ decreased in nasal polyps compared with that of peripheral blood from the same patient (P<0.05). CONCLUSION:There were generous of T lymphocytes infiltrating in nasal polyps. There was abnormal immune status in the local nasal mucosa from the patients, and the predomination of Th cytokine secretion changed compared with peripheral blood from the same patients, which resulted in the change of microenvironment of nasal mucosa and possibly close related to the formation of nasal polyps. 相似文献