若干相同生态位的畜禽寄生蠕虫群落关系分析

在具有相同生态位的畜禽寄生蠕虫构成的２９个种对中,多数不具亲和性,仅２对具有明显亲和性,且均为家畜寄生蠕虫,即寄生山羊瘤胃的鹿同盘吸虫和长菲策吸虫,以及大肠的微管食道口线虫和辐射食道口线虫;种间关系程度则多为负关联,其中完全负关联的有１２对,仅有４对表现出不同程度的正关联;家畜寄生蠕虫的种群值明显高于家禽。分析结果提示,相同生态位的畜禽寄生蠕虫的种间关系普遍存在在负关联的缺少亲和性的现象,寄生空间     

“中苜一号”紫花苜蓿耐盐遗传特性初步研究

以“中苜号”紫花苜蓿耐盐新品种为材料,应用ＮＣⅡ杂交试验方案,研究其耐盐遗传参数,旨在为今后苜蓿耐盐育种提供理论依据。结果表明,在直 迫下,苜蓿的干重、株高和分枝数诸性状的加性产应大于显性效应,狭义跗力较大,分别为０．４２５、０．３８７和０．４８４,具有通过选择进一步提高耐盐性的遗传潜力。     

Diversity of methodologies to experiment Integrated Pest Management in arable cropping systems: Analysis and reflections based on a European network

Integrated Pest Management (IPM) aims to promote physical and biological regulation strategies that help farmers contain populations of pests (pathogens, animal pests and weeds) and to finally reduce the reliance on pesticides. It is based on the holistic combination of multiple management measures rather than on the sum of single methods, each of them having only small effects on pests reduction. Thus, to analyse the interactions between IPM measures and to evaluate the sustainability of their implementation, we require an approach considering the whole cropping system (CS), i.e. a functional entity whose complexity is more than the sum of its parts. A network of European experiments at the CS level was set up recently, and aimed at sharing data and expertise to enhance knowledge of IPM. Comparison of existing methodologies highlighted a diversity of CS designs and experimental layouts. We deduced that the concept of CS itself was viewed differently among scientists, and this affected experimental protocols. Other differences were related to the research context and objectives. Some experiments aimed to explore very innovative strategies and generated knowledge on both their effects on the agroecosystem and their ability to satisfy a set of performance targets, while others aimed to provide quickly adoptable solutions for local farmers in line with the current socio-economic constraints. In some research programmes, the experiment was part of the CS design process — and tested CS were regularly revised based on an continuous improvement loop — while in other cases CS were kept stable across years so as to enable the evaluation of their long-term cumulative effects. A critical aspect contributing to the diversity among CS experiments was the distinction between a factorial design of experimental CS and systemic approaches: factorial experiments allowed quantification of the effects of each IPM component regardless of the consistency between components defining the CS. In contrast, systemic approaches focused on the overall evaluation of CS designed with consideration of their consistency, hence maximising their ability to meet the objectives. Because CS experiments represent a huge investment in terms of economics and time, preliminary reflections of the relevance of the experimental strategy is of critical importance.     

Bioinformatics Analysis of gB Gene of Porcine Lymphotropic Herpesviruses Strain GD27

The glycoproteins B (gB) gene of porcine lymphotropic herpesviruses 2 (PLHV-2)was amplified by PCR and sequenced, then bioinformatics analysis were conducted. The gB protein was composed of 876 amino acids, the molecular formula was C₄₅₀₀H₆₉₇₅N₁₁₉₉O₁₃₅₁S₄₀, the molecular mass was 100.77 ku, the value of theoretical isoelectric point was 6.45, and the instability index was 38.58. The prediction of secondary structure revealed that some alpha helixes and beta sheets exist in protein while random coil was major pattern. It had a signal peptide in the position 1-39 amino acids and a transmembrane helice in the position 761-780 amino acids. It contained several prosites and several intrinsically disordered proteins. Tertiary structure model of gB protein showed a fusion loop. Multiple antigenic epitope located in gB through comprehensive analysis prediction method. It was suggested that gB gene could be as a candidate antigen for vaccination of PLHV-2.     

基于SWOT分析的土地复垦模式研究——以克俄露天采矿区土地复垦模式为例

简要介绍了SWOT方法,然后将之应用于克俄露天矿区土地复垦模式的评价中,通过对比分析,将较为复杂的关系通过矩阵形式清晰地表现出来,指明了该复垦模式未来发展的战略方向。研究表明,SWOT方法在土地复垦模式发展方向的表达上是非常直观有效的,在逻辑上也是严密合理的。     

Cloning of Buffalo AQP9 Gene and Analysing its Expression in the Buffalo Tissues

Cloning buffalo AQP9 gene and analyzing its expression in buffalo tissues.A pair of primers was designed according to the released bovine AQP9 sequences in GenBank,which was used to clone buffalo AQP9 gene.The AQP9 gene was amplified by RT-PCR,whose nucleotide sequence and protein structure were analyzed by bioinformatics methods.The expression of AQP9 in buffalo tissues was assayed by Real-time quantitative PCR.The expression of AQP9 gene in buffalo ovary and testis tissue was detected by immunohistochemical staining method.The results showed that the cloned ORF length of buffalo AQP9 gene was 888 bp,which coded 295 amino acids.The results of multiple sequence comparison showed that the nucleotide sequence of buffalo AQP9 shared 99%,90%,97% and 88% homologeous compared with that of Bos taurus,Sus scrofa,Ovis ariessis and Homo sapiens,respectively,while shared 99%,86%,97%,83% homologeous for amino acids,respectively.Phylogenetic tree analysis indicated that AQP9 gene was highly conservative in the evolutionary process.Real-time quantitative PCR results showed that AQP9 gene expressed in buffalo liver,lung,brain,skin,testis and ovary tissues with different levels,had the most abundant expression in liver,followed by in skin and testis,less observed in lung and ovary.The results of immunohistochemical staining showed that the expression of AQP9 protein varied with the development of buffalo ovarian tissue,and gradually enhanced with follicle development.In testicular tissue,AQP9 protein expressed in spermatocyte and leydig cells of developmental stage testis.These results indicated that we had successfully cloned buffalo AQP9 gene sequences.The expression and its function of AQP9 in buffalo ovaries and testes might play an important role in follicle development and spermatogenesis.     

中国图像工程:2015

目的 本文是关于中国图像工程的年度文献综述系列之二十一。为了使国内广大从事图像工程研究和图像技术应用的科技人员能够较全面地了解国内图像工程研究和发展的现状,能够方便地查询有关文献,且向期刊编者和作者提供有用的参考,对2015年度图像工程重要文献进行了综述分析。方法 从2015年在国内15种有关图像工程重要中文期刊的共148期上所发表的2975篇学术研究和技术应用文献中,选取出723篇属于图像工程领域的文献,并根据各文献的主要内容将其分别归入图像处理,图像分析,图像理解,技术应用和综述5个大类,然后进一步分入23个专业小类(与前10年相同),并在此基础上分别进行了各期刊各类文献的统计和分析。结果 根据对2015年统计数据的分析可看出:图像分析正得到最多关注,目标跟踪的文献数量有明显增加,图像分割和边缘检测仍然是研究重点,图像匹配融合及其在遥感测绘等的应用再次成为热点。结论 我国图像工程的2015年研究深度和广度仍在提高和扩大,保持了快速进展的势头。     

Opelug-ontspanning op Boseiendomme

Insect-induced damage to leaves, and the growth phenology, of Olea europaea subsp. africana and Kiggelaria africana near Stellenbosch (33° 56'S, 18° 52'E) were monitored at weekly intervals between July 1980 and June 1981. The average incidence of damaged leaves was greater in K. africana than in O.e. africana (14 vs 10 %, respectively). In both species, leaf-damage appeared to be slightly more than twice as great in January-June than in July-December, and insect attacks on leaves apparently began to intensify after the plants had completed their main growth and production of new leaves in the austral spring. These findings are discussed in relation to the meagre published information on herbivory in the fynbos biome.