Fe~(3+)对鼠尾藻光合呼吸作用和生化组成的影响

研究了不同浓度Fe~(3+)对鼠尾藻光合作用、呼吸作用和体内不同生化组成的影响。结果表明,不同的Fe~(3+)浓度对鼠尾藻真光合速率和呼吸速率均有显著的影响,在0.04 mg/L的Fe~(3+)浓度下鼠尾藻的光合作用能力达到最强,而0.06 mg/L的Fe~(3+)浓度下鼠尾藻的呼吸作用达到最高;不同Fe~(3+)浓度下,鼠尾藻体内叶绿素a浓度和类胡萝卜素浓度变化显著,可溶性蛋白含量和可溶性糖含量变化不显著。     

鼠尾藻围隔生态系中浮游生物的变化

于2008年4—6月在大连市沙岗子村的海参养殖场进行围隔试验,试验设投放高、低密度的鼠尾藻组和不放藻的对照组。结果表明,围隔中共有藻类8门57属76种,优势种为短楔形藻、新月菱形藻和绿枝藻。其中浮游植物密度以对照组为最多,高、低密度鼠尾藻组的差异不大;浮游动物有6大类34种(属),优势种为中华哲水蚤、瘦尾胸刺水蚤等桡足类及桡足类无节幼体,浮游动物密度亦是以对照组为最多,高、低密度鼠尾藻组的数量差异不大。试验期内,低密度组的藻体长度从22.2 cm生长至39.6 cm,平均增幅为78.3%;高密度组的藻体长度从22.2 cm生长至33.9 cm,平均增幅为52.7%,低密度试验组鼠尾藻的生长要好于高密度组。     

鼠尾藻的药理研究进展

综述了鼠尾藻的药理研究进展,为其进一步开发应用提供参考。     

微波辅助提取羊栖菜多酚的工艺研究

以羊栖菜为原料,在单因素试验的基础上,采用四因素三水平正交试验设计,优选微波辅助提取羊栖菜多酚的最佳工艺条件：乙醇体积分数80%,微波功率600 W,浸提温度70℃,料液比1∶10 g·mL-1,浸提时间4 min,此条件下羊栖菜多酚得率可达233%。     

Structural and Rheological Characteristics of Alginate from Sargassum cristaefolium Extracted by Twin Screw Extruder

This study aimed to investigate the effects of process extrusion on the characteristics of Sargassum cristaefolium sodium alginate (SCSA) extracted using twin-screw extruder. Box-Behnken Design (BBD) from response surface methodology (RSM) was established to understand the effects of temperature, screw speed, and pH on the multiple-responses of alginate characteristics including intrinsic viscosity, yield, and molecular weight. The results revealed that temperature, screw speed, and pH significantly affected (P < .05) all responses. The optimum extraction condition was found at temperature of 58.18°C, screw speed of 77.99 rpm, and pH 10.11. At this condition, the response of residence time distribution was 7.07 ± 0.029 min, yield of 34.01 ± 0.12%, intrinsic viscosity of 460.13 ± 14.75 mL/g, and molecular weight of 217.94 ± 7.14 × 10³ g/mol. This alginate had mannuronic acid to guluronic acid (M/G) ratio of 0.28, and the L-guluronic acid block was 0.78, which was higher than the D-mannuronic acid block. Rheological characterization of SCSA in aqueous solution was shear-thinning pseudoplastic, and alginate gel in 1 M CaCl₂ was more elastic than liquid.     

Damage to the Membrane Permeability and Cell Death of Vibrio parahaemolyticus Caused by Phlorotannins with Low Molecular Weight from Sargassum thunbergii

ABSTRACT

To understand the mode of action of low molecular weight phlorotannins (LMPs) from Sargassum thunbergii against Vibrio parahaemolyticus, effects of LMPs on the growth curve and the content changes of some substances in culture media were investigated, and those on morphological and ultramicrostructure were also observed by scanning electron microscope and transmission electron microscopy. The results showed that LMPs inhibited thalli growth at logarithmic phase and damaged the cell membrane and cell wall of tested bacteria which led to the leakage of cytoplasm and the deconstruction of membrane permeability. The present study indicates that LMPs as natural products in seaweeds deserve further investigation for food safety control and aquacultural drugs.     

硇洲马尾藻人工育苗常见敌害生物及防治初探

本文调查了硇洲马尾藻人工育苗期间的主要敌害生物,并初步探讨了不同物理方法和化学药剂对其敌害生物的清除效果,旨在为人工育苗栽培提供基础资料。调查发现硅藻、刚毛藻、石莼、浒苔等附生藻类对硇洲马尾藻生长影响较大,蓝子鱼、麦秆虫和藻钩虾的摄食是硇洲马尾藻幼苗海区培育的主要危害。苗帘用5%硫酸铵药浴5min可抑制刚毛藻的生长,以2%柠檬酸药浴5min、0.3%醋酸药浴3min、3%硝酸铵药浴5min等均可药杀石莼、浒苔等附生绿藻,用640mg/L敌百虫浸泡10min可杀灭藻钩虾。     

羊栖菜非多糖成分及其药理活性研究进展

综述了近年来对羊栖菜岩藻黄质、多酚、萜类化合物、甾醇化合物等非多糖成分的提取分离以及药理活性方面的研究进展,为羊栖菜非多糖成分的进一步研究和开发利用提供参考。     

匍枝马尾藻人工栽培初步研究

为探索匍枝马尾藻(Sargassum polycystum)的人工栽培技术,采用浮筏式栽培方式于2015年3—6月在海南省儋州市海头镇进行了匍枝马尾藻的人工栽培试验,记录了该藻的生长规律,并通过栽培水深和夹苗密度试验,比较了不同栽培条件对其生长的影响。结果显示,3月下旬至5月下旬为匍枝马尾藻生长最旺盛的季节,其中藻体长度的快速增长主要集中在4月中旬以前,而藻体重量以相对稳定的速率持续增长至5月下旬。栽培水深和夹苗密度分别对匍枝马尾藻的藻体长度和分枝数有显著影响,而藻体鲜重则同时受到这两个因素的影响(P0.05)。研究表明,适量增加匍枝马尾藻的栽培水深和控制夹苗密度有利于促进其生长;为有效提高匍枝马尾藻的产量,栽培水深应设计为80 cm左右,夹苗密度控制在10 cm左右。     

铜藻粗多糖酶法提取工艺的优化及其抗氧化活性研究

通过正交试验法对铜藻中粗多糖的酶法提取工艺进行优选,并测定其1,1-二苯基苦基苯肼（DPPH）自由基的清除能力、超氧阴离子自由基（O2-）及抗脂质过氧化能力;优化后铜藻粗多糖提取率为5.61%,具有一定的DPPH自由基清除能力、抗脂质过氧化能力、O2-清除能力。