沙漠地区农业种植技术初探

沙漠地区在没有造林或造林初期防护效益不显著情况下，农业种植受风沙危害比较严重，提高农业种植产量是我们面临的一道难题。本文对中国林科院沙漠林业实验中心井灌区第四实验场风沙环境和三年来的种植情况进行分析，得出农业防风害的措施有以下几个方面：农作物品种的选择；种植时间的调整；以增加起动风速为目的的各项抗风害措施的采用；以减小风沙流强度和目的的阻沙林和固沙工程的建造。这些措施能有效地减小和防止风沙灾害的发     

植物性抗生育剂防治幼林地兔害研究

在山西省吕梁山国有林管理局下李林场新造油松和侧柏混交林内,以20m×40m的面积投放一堆植物性抗生育剂和红胡萝卜混合药饵200g,对照地不放药。通过对施药前后试验地与对照地兔粪和苗木为害率调查数据进行统计分析,结果表明,与对照相比,投放植物性抗生育剂能显著减少幼林地内野兔活动频率和对苗木的为害。     

Splenectomy enhances sensitivity of islets to STZ in mice

AIM: To explore the protective role of spleen in damage of islet beta cells induced by streptozotocin (STZ).METHODS: Splenectomy in mice was performed by surgery. Sixty splenectomy mice were divided into 3 groups as the mice were intraperitoneally injected with STZ at doses of 80 mg/kg or 160 mg/kg, and saline, respectively. Sixty normal mice (without splenectomy) were also grouped and treated as above for controls. One week later, fasting blood glucose and serum insulin were monitored,and beta cell mass and the apoptosis of islet cells were analyzed by the methods of immunohistochemistry and ELISA, respectively. The content of reactive oxygen species was determined by the method of luminol-enhanced chemiluminescence.RESULTS: Compared to the normal control mice, the concentrations of fasting blood glucose significantly increased, and serum insulin reduced in splenectomy mice treated with STZ at the dose of 80 mg/kg. Moreover, beta cell mass decreased, enrichment factor of nucleosomes of islet apoptotic cells and reactive oxygen species produced in pancreas tissues significantly increased in splenectomy mice treated with STZ at the dose of 80 mg/kg.CONCLUSION: Splenectomy increases the sensitivity of islets to STZ in mice by increasing the production of reactive oxygen species in pancreas.     

不同化肥农药减施组合对水稻主要病虫害发生及产量的影响

为探索化肥农药减施融合使用技术，设计肥料和农药两因素三水平试验，研究不同化肥农药减施组合对水稻主要病虫害发生及产量的影响。结果发现：减肥有减轻病虫发生程度的趋势，减药有加重病虫发生程度的趋势；减肥可显著影响水稻纹枯病、第4代稻飞虱、稻纵卷叶螟发生程度，减药可显著影响水稻纹枯病、第3和4代稻飞虱、稻纵卷叶螟发生程度，但两者均对稻瘟病、稻曲病、二化螟发生程度无显著影响。减肥30%时实测减产10.29%，对产量影响显著；减肥低于20%时，绿色助剂替代处理和生物农药替代处理实测产量比对照处理增产0.38%和2.49%，生物农药替代和绿色助剂替代均可作为化学农药减量的技术措施。     

基于形态学的棉花种子破损检测

以"中棉35"棉花种子为对象,应用图像处理技术,提出了基于形态学的破损识别方法.采用链码算子获取轮廓信息并进行傅里叶算子平滑,根据曲率特征定位轮廓尖端点后,通过轮廓的对称性识别局部破损棉种;基于统计思想,根据大小、形状特征的差异识别严重破损棉种.选取完好、破损棉种各200粒进行识别试验,结果表明破损棉种识别率达到94%.     

Activation of repair pathways after neuronal DNA damage induced by bupivacaine

AIM To investigate the activation of related repair pathways after bupivacaine-induced neuronal DNA damage by cDNA gene screening. METHODS The bupivacaine-induced SH-SY5Y neuronal damage and DNA damage model was established. The technique of cDNA microplate array was used to screen the 21 important regulatory factors in the DNA damage repair pathway. Post-analysis of these differentially expressed repair genes for the repair pathway enrichment and distribution was performed. The data were analyzed by GraphPad Prism 6 statistical software to compare differences between groups. RESULTS The viability of SH-SY5Y cells treated with bupivacaine at different concentrations （detected by CCK-8 assay） showed that the IC₅₀ value of bupivacaine was 1.5 mmol/L. The comet assay related index （the comet tail） was increased （P<0.05）， the phosphorylation level of γH2AX protein was increased （P<0.05）， indicating that DNA damage in the SH-SY5Y cells was significantly aggravated after bupivacaine treatment. The results of cDNA microplate assay showed that compared withcontrol group， the differentially expressed genes after bupivacaine treatment were DNA-PKcs， PTEN， NTH1， RAD9， CSB， GADD45， XPD， XPC-HR23B and P53. The analysis showed that these repair genes were mainly concentrated in the following 3 repair mechanisms： base excision repair， nucleotide excision repair， and non-homologous reconstitution. CONCLUSION The repair genes differentially expressed after neuronal DNA damage caused by local anesthetics are mainly concentrated in the pathways of non-homologous end-joining， base excision repair and nucleotide excision repair.     

毕节地区新造林地鼠兔危害状况调查及防控对策

对啮齿类动物鼠兔害的防治.危害调查、标本制作、种类鉴定、生活习性观察,探索有效控制措施.本文着重从森林环境资源的核算评估鼠兔害对新造林地经济损失.     

露地大白菜低温冻害气象指数保险费率厘定方法研究

【目的】为科学厘定露地大白菜低温冻害保险费率提供方法,为其他气象因子指数保险的保险费率厘定提供参考,为政策性农业保险的可持续发展提供助力。【方法】将1957—2018年每年初、终霜冻日期转化为儒历日,计算平均初、终霜冻日期,将平均初、终霜冻日期设定为低温冻害指数保险中的时间起赔阈值,并利用标准差分级法,对起赔阈值设置的合理性进行检验。精确统计大白菜各生长期投入成本,根据灾损率进行加权计算得出赔付比例。通过计算发生概率、灾损率与赔付比例3个因子综合得出露地大白菜低温冻害保险费率,并对种植区域进行加权计算,得出区域内的露地大白菜低温冻害保险费率。【结果】设计了露地大白菜低温冻害气象指数保险中时间和气温起赔阈值的计算方法,并制定了时间起赔阈值的检验方法。设计了低温冻害保险费率3个决定因子发生概率、灾损率和赔付比例的计算方法。【结论】经过检验,将平均初、终霜冻日期设定为丰宁满族自治县地区露地大白菜低温冻害气象指数保险的时间起赔阈值是合理的。露地大白菜在遭受轻、中、重三级低温冻害时的灾损率分别为0.3、0.7和1.0;发芽期赔付比例最高为45%,秧苗期赔付比例最高为75%,莲座期以后赔付比例最高为100%。各乡镇露地大白菜低温冻害气象指数保险费率为2%～3%,经过加权计算得出丰宁满族自治县地区低温冻害保险费率为2.14%。     

Depletion of ascorbic acid derivatives in fish feed by the production process

ABSTRACT:   The depletion of ascorbic acid derivatives in fish feed during the feed processing (extrusion cooking and drying) was studied at five extrusion cooking temperatures and at 85°C for 2 h dryer processing temperature. Three ascorbic acid derivatives were used: L-ascorbyl-2-mono-phosphate Mg (APM), L-ascorbic acid sodium (AAS) and L-ascorbic acid palmitic acid ester (AAP). Samples were collected after drying and ascorbic acid derivatives losses evaluated. APM was found to be quiet stable with an average retention of 88%, but AAS and AAP were unstable and the depletion was very high.     

玉米种子的机械损伤对其发芽率的影响

玉米种子质量是决定玉米产量的重要因素.目前,由于我国没有生产专用种子玉米脱粒机,玉米种子的脱粒只能采用打击原理设计的普通脱粒机,对玉米籽粒伤害较大,从而影响了种子在田间的出苗率以及植株整个生长发育过程.为此,针对不同部位有机械损伤的玉米种子对其发芽率的影响进行了分析,进而为新型低损伤玉米种子脱粒机的研制提供作用部位与作用方向的参考.