口蹄疫病毒结构蛋白P1基因转化玉米的初步研究

实验在构建口蹄疫病毒(Foot and Mouth Disease Virus,FMDV)结构蛋白P1基因植物双元表达载体(pBI131SP1、pBI21P1和pBI121AP1)的基础上,以玉米自交系8902、340、4112的Ⅱ型胚性愈伤组织为受体,用农杆菌介导法和基因枪轰击法转化玉米,获得31株抗性再生植株.GUS染色证明外源目的基因在玉米细胞和组织中得到了表达.PCR检测、Southem blotting鉴定证实目的基因P1已整合到再生植株的基因组中,共获得了13株转基因玉米植株,首次进行了FMDV抗原基因转化玉米的研究。     

黑木耳原生质体再生株选育高产菌株研究

用1.5%溶壁酶将黑木耳双核菌丝脱壁再生。再生菌株经培养、观察、栽培及遗传特性研究后,证明有变异产生。现已从中选出一株优质、高产新菌株,且已用于生产。     

Variation in Spikelet-Related Traits of Rice Plants Regenerated from Mature Seed-Derived Callus Culture

Abstract

Callus is An Excellent Source For in Vitro Plant Regeneration, But Plants Regenerated from Callus Sometimes Show Phenotypic And Genotypic Variation from The initial Plants. in This Study, The Variation in Spikelet-Related Traits of The Rice Plants Regenerated from Calluses And Their Performance in The Paddy Field Were Examined. The Phenotypic Variation in Spikelet-Related Traits of The Regenerated Plants Was Not Always in A Reduction in Their Mean Value. For instance, Panicle Length, Spikelet Number And Fertile Spikelet Number of indonesian Rice Genotypes Ciapus And Bp-140 in The Regenerated Plants Were Significantly Greater Than Those of The initial Plants (Developed from The Seeds). The Spikelet Fertility of The Regenerated Rice Plants Was Not Significantly Lower Than That of The initial Plants Except in Ciapus And Bp-140. The Occurrence of Somaclonal Variants Varied With The Genotype. Ciapus And Bp-140, Which induce Many Somaclonal Variants, Are Suggested To Be Valuable For Genetic, Breeding Or Functional Genomic Studies, While Fatmawati, Which is Stable, Could Be Used For Genetic Transformation Study.     

烟草NC89叶肉细胞原生质体再生植株的遗传稳定性

对烟草 Ｎ Ｃ８９ 叶肉细胞原生质体再生植株的 Ｒ０、 Ｒ１、 Ｒ２ 及 Ｒ３ 代的农艺性状进行了观察比较，测定了各代叶片中蛋白质和可溶性还原糖的含量，比较了各代植株的过氧化物同工酶谱，发现再生植株及其后代在农艺性状、蛋白质含量上均具有较好的遗传稳定性，过氧化物同工酶谱随代数的延长而趋于相同。     

The role of different sources of tree regeneration in the initial stages of natural forest recovery after logging of conifer plantation in a warm-temperate region

We analyzed the role of regeneration type (surviving from advanced regeneration, resprouting from advanced regeneration, or newly recruited seedlings) in determining the quantity and quality of individuals in reestablishing a natural forest following clear-cutting of a conifer plantation. We experimentally clear-cut a plantation and undertook a detailed tree census before and after clear-cutting. After clear-cutting, a species composition partially representing warm-temperate evergreen broadleaf forest was present in the form of surviving and resprouted individuals originating from advanced regeneration that had established in the Chamaecyparis obtusa plantation. On the other hand, seedlings that established after clear-cutting were dominated by pioneer species, and the number of seedlings was far greater than the number of individuals originating from advanced regeneration. Our results suggest that the role of regeneration type would differ in relation to the objectives for forest recovery. When any kind of woody cover of the ground surface after clear-cutting would suffice, we suggest that the contribution of pioneer seedlings originating from buried seeds would be important. If the recovery objective is to form a simple evergreen broadleaf forest, such as a coppice woodland, all three sources of regeneration would play a similar role, provided the understory before clear-cutting was as well developed as the one in this study. If rapid establishment of a lucidophyllous forest with diverse species composition is desired, the role of the surviving and resprouting individuals originating from the advanced regeneration would be much more important.     

震后关于农村住宅建设的思考与对策

通过对当前农宅状况的概括以及今后农村住宅建设的思考，针对震后产生的建筑废物所带来的环境压力以及住宅在抗震性能方面的要求，提出了将再生混凝土和钢结构住宅体系应用于今后农村住宅建设中的设想。     

大白菜花药培养胚状体诱导研究

以9个大白菜一代杂种为材料进行花药培养。结果表明,大白菜胚状体诱导率受基因型、预处理时间等因素影响。花药在接种后的15～30 d出现胚状体,最后得到了再生植株。     

心叶驼绒藜胚器官培养及再生体系的研究

以心叶驼绒藜无菌苗叶片、茎段、幼根为外植体,对通过器官发生的途径发育成再生植株进行了研究。结果表明：（1）、无菌苗的建立：用1/2MS＋琼脂＋蔗糖,出苗率高（90%以上）、苗壮。（2）、诱导培养基：用MS1＋IAA0.2mg/L＋KT0.1mg/L培养基,出愈率低,没有获得再生植株;用MS2＋IAA0.2mg/L＋KT0.2mg/L、MS3＋IAA0.1mg/L＋KT0.1mg/L培养基,叶片、茎段具有再生植株发育的能力,出愈率较高。（3）、生根培养基：用1/2MS＋NAA0.1 mg/L,生根率高、周期短;用1/2MS＋IAA0.1 mg/L,生根率低,周期长。     

Ti质粒介导的B、t、k-δ内毒素蛋白基因转化大豆的初步研究

以Ｔｉ质粒为介导，将ｐＫＴ５４Ｂ７Ｃ５质粒上的Ｂ、ｔ、ｋ－δ内毒素蛋白基因导入东北大豆“黑农３７”、“黑农３９”等品种。采用多种外植体和感染方法，从胚轴和子叶节诱导出丛生芽与再生植株。经卡那霉素筛选和冠瘿碱检测，初步证明外源基因导入大豆基因组中。共获得８１株再生植株，其中成活３０株，仅３株结７个荚，得到７粒种子。ＰＣＲ检测和ＤＮＡ分子杂交，鉴定这７株再生植株呈阳性反应，证明外源基因已导入并整合到大豆的基因组中。７粒种子均已萌发，植株生长发育正常     

金樱子的组织培养与快速繁殖

以金樱子当年生幼嫩带芽茎段为外植体,建立了从启动培养、丛生芽的诱导和增殖、生根、壮苗、炼苗到移栽技术体系,可用于人工培育金樱子种苗。