Effect of Limestone of Different Sizes on Soil Extractable Phosphorus and Its Concentrations in Grass and Clover Species

Phosphorus (P) is often reported as a nutrient whose availability increases by lime application, although some research suggests a decline in the short-term P availability upon liming. This study addresses the effects of applying different sized limestone (2–4, 0.5–2, 0.25–0.5, and <0.25 mm) on the forms and availability of soil P, P concentrations and extraction by pasture, and yields of plant species. Soil P was partitioned into organic and inorganic forms, using an ignition method. Available P was determined by the Olsen and Mehlich 3 methods. Phosphorus in plants was determined after acid digestion. Most P was present in organic form. One year after sowing, inorganic P increased and organic P decreased. Liming had no significant influences on soil P. Phosphorus extracted by Mehlich 3 explained 40% of the total dry-matter yield in the first harvest. No significant effect of liming on P concentration in plants was observed.     

Soil‐Test Methods for Florida Sands Treated with an Aluminum–Water Treatment Residual and Various Phosphorus Sources

Use of aluminum (Al)–rich water treatment residuals (Al‐WTR) has been suggested as a practice to immobilize excessive phosphorus (P) in Florida soils that could represent an environmental hazard. Fertilizer P requirements can differ in WTR‐amended and unamended soil, so careful selection of soil‐testing methodology is necessary. Acidic extractants can dissolve WTR sorbed P and overestimate plant‐available P. We evaluated the suitability of the Mehlich 1 P (M‐1P) and other agronomic soil‐test procedures in an Al‐WTR‐treated Florida soil. Bahiagrass (paspalum notatum Fluggae), ryegrass (Lolium perenne L.), and a second bahiagrass crop were grown in succession in a Florida topsoil amended with four sources of P at 44 kg P ha^?1 (P‐based rates) and 179 kg PAN ha^?1 [nitrogen (N)–based rates] and three WTR rates (0, 10, and 25 g kg^?1 oven‐dry basis). Both water‐extractable P (WEP) and iron (Fe) strip P (ISP), but not M‐1P, values of soil sampled at planting of each grass were greater in the absence than in the presence of WTR. Total plant P uptake correlated with WEP (r² = 0.82^***) and ISP (r² = 0.75^***), but not M‐1P (r² = 0.34^***). Correlations of the dry‐matter yield, P concentration, and P uptake of the first bahiagrass were also better with WEP and ISP than with M‐1P values. However, regression of plant responses with M‐1P improved after the first crop of bahiagrass. Both WEP and ISP values were better predictors of available soil P than M‐1P in a field study with same four P sources surface applied to established bahiagrass at the same two P rates, with and without WTR. Both WEP and ISP are recommended as predictors of P adequacy in soils treated with WTR, especially for soils recently (< 5 months) treated with Al‐WTR.     

P16基因在妊娠滋养细胞肿瘤中的表达及其意义

目的：探讨抑癌基因Ｐ＾１６的表达水平与妊娠滋养细胞肿瘤的形成及转移倾向的相关性。方法：采用免疫组织化学方法，检测葡萄胎３０例，侵蚀性葡萄胎２５例，绒毛 ２６例及正常绒毛２５例的滋养细胞中Ｐ＾１６的表达。结果：（１）Ｐ＾１６在妊娠滋养细胞肿瘤中有较高的表达，阳性率为７４．５％（其中侵蚀性葡萄胎７２．０％，绒毛膜癌７６．９％），高于葡萄胎组（２０．０％）和正常绒毛组（１６．０％），差异有高度显著性；（     

杀虫剂胁迫对小白菜B. campestris体内营养物质和黄曲条跳甲P. striolata取食的影响

采用生物测定与生理生化方法，研究不同杀虫剂胁迫下小白菜B. campestris体内的蛋白质和可溶性糖含量变化，以及对黄曲条跳甲（P. striolata）取食的影响。结果显示，杀虫剂处理对小白菜体内蛋白质含量的影响不显著，但对小白菜体内可溶性糖含量的影响显著。统计分析表明，杀虫剂处理后，黄曲条跳甲对小白菜的取食与植株体内蛋白质含量呈显著的负相关（P〈0.05），与小白菜的可溶性糖含量呈极显著的正相关（P〈0.01）。     

五味子培育工艺的应用与发展前景

五味子药用植物资源在我省十分丰富，通过科学培育，实现产业化，从而促进我省经济结构的调整，发挥这一产业在全省经济发展中的作用。     

Establishment and application of cell model for P2ry2 modulators screening based on CaCC

AIM To construct a high-throughput screening cell model for P2Y₂ purinergic receptor （P2ry2） modulators based on calcium-activated chloride channel （CaCC）. METHODS The mRNA expression of P2ry2 in Fischer rat thyroid （FRT） cells was detected by RT-PCR， and the PCR products were collected and sequenced. The protein expression of P2ry2 in the FRT cells was also detected by Western blot. The eukaryotic expression vectors ANO1 and YFP-H148Q/I152L were constructed. The FRT cells co-expressing ANO1 and YFP-H148Q/I152L were obtained by liposome transfection， antibiotic screening and limited dilution. The expression of ANO1 and YFP-H148Q / I152L in the cells was observed under fluorescent inverted microscope. The validation of the cell model for screening P2ry2 modulators was verified by the fluorescence quenching kinetics tests， and intracellular free calcium was analyzed by Fura-2 staining to investigate the dose-dependent relationship between intracellular calcium concentration and P2ry2 modulators. Z' factor was applied to evaluate the stability and repeatability of the cell model. RESULTS P2ry2 were endogenously expressed in the FRT cells. The expression of ANO1 on the cell membrane and the expression of YFP-H148Q/I152L in the cytoplasm were observed under fluorescent inverted microscope. The cell model was successfully constructed. The fluorescence quenching kinetics test confirmed the cell model for screening P2ry2 modulators was constructed successfully， and the calcium concentration in cytoplasm was increased rapidly after the addition of a small amount of P2ry2 agonist， indicating that the cell model was sensitive for detecting the calcium concentration in cytoplasm. The calcium concentration in cytoplasm， P2ry2 modulators and the slope of fluorescence change were in a dose-dependent manner， respectively. The Z' factor was 0.75， indicating that the established cell model was able to use for high-throughput screening of P2ry2 modulators with excellent stability and repeatability. CONCLUSION A simple， economical， and efficient cell screening model of P2ry2 modulators is successfully constructed， which is suitable for the detection and evaluation of P2ry2 modulators.     

化学镀Fe-Al-P合金初探

利用化学镀方法在铜基片上施镀了Ｆｅ－Ａｌ－Ｐ合金镀层。通过改变金属盐比率ＡｌＣｌ３／（ＡｌＣｌ３＋ＦｅＳＯ４）、金属盐ＡｌＣｌ３和还原剂ＮａＨ２ＰＯ２的浓度等参数，研究了合金镀层的沉积速率，成分，表面形貌及结构的变化规律，得到了最佳镀液配方，发现络合剂和还原剂的选择是提高镀层含Ａｌ量的关键。     

用8-羟基喹啉硫酸盐防止梨、苹果外植体褐变

以金花梨（PyrusbretschneideriRehd．）、金冠（MaluspumilaMill．）和富士（M.pumilaMill．）苹果2～3mm茎尖为外植体，用8-羟基喹啉硫酸盐（8－Hydroxy－Quinolinol－Sulfate，8－HQS）进行处理后，接种在MS＋2．0mg·1-1BA＋0．2mg·1-1IBA培养基上，结果表明，外植体接种后用0．1％的8-HQS淹没24h，然后再将外植体转移至新鲜培养基上，能有效地防止金花梨和金冠苹果外植体褐变（褐变率65％～75％）；外植体消毒前用0．1％的8－HQS预处理24h与接种后用0．1％的8－HQS处理24h对防止富士和金冠苹果外植体褐变具有同样效果（褐变率50％～85％）；金冠与富士苹果外植体消毒前用0．1％和1．0％的8－HQS预处理12h与24h褐变率差异不显著（相差0．6～1．0个百分点），建议用1．0％的8－HQS预处理12h。     

牡丹和芍药雄蕊筒状花瓣的形态解剖特征及形成

对牡丹(Paeonia suffruticosa)、紫斑牡丹(P.rockii)、大花黄牡丹(P.ludlowii)、四川牡丹(P.decomposita)、杨山牡丹(P.ostii)、卵叶牡丹(P.qiui)、滇牡丹(P.delavayi)和芍药(P.lacti- flora)等8个分类群中雄蕊瓣化瓣的形态特征进行了观察,并对仅出现在牡丹、紫斑牡丹和芍药花中的雄蕊筒状花瓣进行了统计分析和形态解剖学研究。结果表明:(1)在牡丹和紫斑牡丹中雄蕊筒状花瓣出现的频率低,且形态单一;而在芍药中其出现频率较高,且形态多样。(2)在解剖结构方面,雄蕊筒状花瓣从基部到顶部存在一系列的转化,与牡丹和紫斑牡丹相比,芍药中筒状花瓣的解剖结构更具多样性。(3)维管束边缘分生组织的不断分化和维管束数量的不断增多,在雄蕊原基转向雄蕊简状花瓣的发育中起到关键作用。(4)雄蕊筒状花瓣的发育过程可分为原基分化、转化发育、筒状花瓣形成和筒状花瓣成熟4个阶段。     

上海青氮肥追施减量增效试验

为研究追肥减氮对上海青生长和土壤理化性状的影响,设置空白对照、常规施肥、追肥减氮10%、追肥减氮15%、追肥减氮20%、追肥减氮25%、追肥减氮30%7个处理。试验结果表明,追肥减氮25%时,上海青鲜质量和品质指标显著高于对照和其他处理;土壤和植株氮、磷、钾等有效养分含量显著高于对照和其他处理,说明在一定范围内,随着氮肥施用量的降低,其利用率反而增加,因此在栽培过程中合理施用氮肥能够有效提高肥料利用率。