养分专家系统推荐施肥对冬小麦产量、养分转运及肥料利用的影响

为探究冬小麦科学施肥技术,明确养分专家系统推荐施肥对冬小麦产量、养分积累转运与利用的影响,于2018—2019年分别在河南省鹤壁市和新乡市以冬小麦(鹤壁、新乡试验品种分别为‘郑麦7698’和‘郑麦366’)为试验材料,设置7个处理[农民习惯施肥(FP)、当地推荐施肥(ST)、养分专家系统推荐施肥(NE)、在NE基础上配施缓控释氮肥(RNE)、在NE基础上不施氮肥(NE-N)、在NE基础上不施磷肥(NE-P)、在NE基础上不施钾肥(NE-K)],探究不同施肥处理对冬小麦氮、磷、钾养分转运分配规律和肥料利用效率的影响。结果表明,冬小麦氮、磷、钾施肥量NE较FP处理分别降低16.2%、43.3%、-13.2%(鹤壁)和19.5%、48.0%、-57.9%(新乡);冬小麦产量NE与FP处理无显著性差异,RNE与FP处理存在显著性差异;NE、RNE较FP处理分别增产4.7%～6.6%、5.5%～9.6%(P0.05)。进一步研究表明,NE、RNE处理可显著增加地上部植株养分含量和积累量(P0.05),其花后干物质积累量较FP处理增加9.2%～14.0%、11.9%～18.6%;花前氮、磷素转运量和钾素转运对籽粒钾素积累贡献率均有提高,基于养分专家系统推荐施肥的氮、磷、钾肥平均利用效率分别为42.1%、19.2%、46.6%,平均农学效率分别为11.5kg·kg~(-1)、13.2 kg·kg~(-1)、13.3 kg·kg~(-1)。综上可知,小麦养分专家系统指导优化了氮、磷、钾肥的施用量和施用方法,促进了小麦对氮、磷、钾养分的吸收利用,提高了肥料利用率,具有良好的增产效果,可以在河南地区推广应用。     

盐份浓度对油菜干物质积累分配、农艺性状及品质的影响

为了研究不同土壤盐份浓度对油菜生长的影响，2015—2016年度以‘扬油9号’和‘苏油211’为供试品种，在低盐浓度(2.978 g/kg)和高盐浓度(4.900 g/kg)试验田进行种植，于成熟期取样测定植株干物质积累量、农艺性状及籽粒品质含量。结果表明，随着盐份浓度增加，全株及不同器官干物质积累量均显著降低，高盐与低盐处理相比，‘扬油9号’和‘苏油211’产量下降幅度分别为24.14%和27.83%，不同器官中根系干物质积累量下降幅度最大，‘扬油9号’和‘苏油211’根系干物质下降幅度分别为34.52%和39.69%，茎枝干物质积累下降幅度最小，两品种下降幅度分别为9.46%和12.93%。随着盐份浓度增加，茎枝干物质分配比例显著增加，其余器官干物质分配比例均显著降低。随着盐份浓度增加，株高和角果数显著降低，而每角粒数和千粒重在不同盐份浓度处理间无显著差异。随着盐份浓度增加，籽粒油份含量显著降低，蛋白质含量显著增加。     

Can differences in 15N natural abundance be used to quantify the transfer of nitrogen from legumes to neighbouring non-legume plant species?

Natural variations in the stable isotope ¹⁵N are often exploited in studies of N cycling in ecosystems. Lower ¹⁵N natural abundance in non-legume plants growing in association with legumes, compared with the non-legume grown alone in pure stands have been observed in cropping, forage, and agroforestry systems. Such observations have frequently been attributed to the transfer of biologically-fixed nitrogen (N) from the legume to the companion non-legume, and various methodologies have been employed to calculate the extent of the N transfer. While some of these ¹⁵N natural abundance-based estimates of N transfer were within the range previously reported using equivalent ¹⁵N-enriched techniques (<20% of non-legume plant N and <10 kg N ha⁻¹ derived from fixed N contributed by neighbouring legumes), many of the values obtained using natural abundance were much higher (30%–83% of the non-legume N derived from fixed N representing up to 30–40 kg N ha⁻¹) than generally measured by ¹⁵N-enriched methods; with even greater estimates being determined where data were available to allow N transfer to be re-calculated on the basis of total legume N rather than fixed N (42% to >100%, and up to 110 kg N ha⁻¹ per year). This review raises concerns about the assumptions behind the natural abundance approach, and provides some alternative interpretations for the observed differences in natural ¹⁵N abundance between plants grown in the presence and absence of legumes. It was concluded that simple comparative measures of non-legume δ¹⁵N alone cannot provide a quantitative estimate of N transfer between plant species if the dominant source and the isotopic identity of the transferred N cannot be validated, and if the extent of any isotopic fractionation associated with relevant N transformations occurring during transfer cannot be defined. To date this information is not forthcoming. There is a need to greatly improve our understanding of the transfer processes before the real value of the δ¹⁵N technology can be realized. In the first instance this will primarily be achieved by carefully executed experiments under controlled conditions, and in the field, employing both ¹⁵N natural abundance and enrichment approaches so estimates of transfer can be compared, and the data interrogated using modelling approaches to explore isotopic fractionation.     

适雨灌溉下不同施肥模式对机插稻稻田水环境及产量的影响

【目的】探讨江汉平原地区适雨灌溉条件下不同施肥模式对机插稻稻田水环境及水稻生长的影响,为当地机插稻水肥管理措施的改善提供理论和数据支撑。【方法】采用田间小区试验,研究了适雨灌溉条件下,农民习惯施肥(FFP)、70%控释掺混肥+30%尿素(70%CRF+30%N)和有机无机复混肥(OIF)对稻田降雨利用率、田面水氮磷质量浓度的动态变化与径流流失量、干物质积累及水稻产量的影响。【结果】适雨灌溉下,返青期、分蘖期、拔节孕穗期和灌浆成熟期的降雨利用率分别为17.5%、100%、100%和84.2%;基肥和分蘖肥施用过后,FFP、70%CRF+30%N和OIF处理田面水TN、NH4+-N和TP质量浓度迅速提高,在第1天达到峰值,水稻移栽后30 d内70%CRF+30%N处理田面水TN、NH4+-N和TP的平均质量浓度较FFP处理分别降低40.4%、47.4%和0.5%;稻田氮磷径流流失量的90%左右在返青期,10%左右在灌浆成熟期,70%CRF+30%N处理TN、NH4+-N和TP径流损失量较FFP处理分别降低31.4%、30.9%、1.9%;70%CRF+30%N处理在返青期干物质积累量显著低于FFP和OIF处理,移栽-返青期阶段干物质积累量占总积累量比例表现为FFP处理>OIF处理>70%CRF+30%N处理,成熟期OIF处理干物质积累量显著高于70%CRF+30%N和FFP处理,实际产量表现为OIF处理>70%CRF+30%N处理>FFP处理。【结论】适雨灌溉条件下,70%CRF+30%N处理有助于减少稻田氮素流失,OIF处理有助于机插稻干物质积累与产量的增加。     

淮北地区氮肥群体最高生产力水稻钾素吸收利用特征

以淮北地区有代表性的34个中熟中粳品种为试材,设置7个氮肥水平(0、150.0、187.5、225.0、262.5、300.0、337.5 kg hm~(–2)),得出各品种的最高产量,将该最高产量定义为氮肥群体最高生产力。在此基础上,明确处于顶层水平(≥10.50 t hm~(–2))、高层水平(9.75~10.50 t hm~(–2))、中层水平(9.00~9.75 t hm~(–2))和底层水平(≤9.00 t hm~(–2))水稻品种的钾素积累、分配及转运特征。结果表明,4个生产力等级水稻品种地上部植株、茎鞘和叶片的含钾率在拔节期最高;抽穗期顶层水平品种的这3个参数高于其他3个等级的品种;穗部含钾率差异不显著。随着氮肥群体生产力等级的提高,钾素总积累量增多;拔节前底层水平钾素积累量最多,两年平均为120.56 kg hm~(–2),比例占50.56%,顶层水平为最少,两年平均为108.02 kg hm~(–2),比例占35.99%;拔节至抽穗期和抽穗至成熟期顶层水平钾素阶段积累量及比例显著高于其他3个等级。移栽至拔节期,钾素积累速率为中层底层高层顶层水平,拔节后则为顶层高层中层底层水平。叶片的钾素转运量及转运率明显高于茎鞘;顶层水平叶片的钾素转运量高于其他3个等级,高层水平叶片的转运率最高;穗部增加量随生产力等级的递增而变大;抽穗到成熟期,茎鞘、叶片对穗的钾素转运贡献率表现为底层最高,中层次之,顶层最低。4个等级水稻品种籽粒生产率和百千克籽粒吸钾量差异不显著;钾素偏生产力和钾收获指数均表现为顶层高层中层底层水平。总之,氮肥群体最高生产力越高,水稻中后期植株钾素积累量及器官对钾素的吸收利用效率越显著。抽穗后保持较高的钾素吸收利用及转运效率是高产水稻品种的重要特征。     

施氮深度对‘黄金梨’树氮素吸收、分配及利用效率的影响

以7年生‘黄金梨’树为试材,采用~(15)N示踪技术研究了3个施氮深度[0(表面施氮)、20和40 cm]处理下不同器官对氮素的吸收、分配及利用效率,并探讨了氮素在土壤中的残留及损失。结果表明,梨果实成熟期,果实的Ndff值最高,其他器官的Ndff值均表现为20 cm施氮深度处理显著高于其他处理。各施氮深度处理,~(15)N的分配率均为贮藏器官最高,生殖器官最低。20 cm施氮深度处理的梨树N利用率最高(26.23%),40 cm施氮深度处理最低(15.65%)。N损失率以表面施氮处理最高(54.21%),20 cm施氮深度最低;0~80 cm土层的N残留率以40 cm施氮深度处理最高(31.73%),表面施氮处理最低。因此,本研究中,20 cm深度施氮处理能提高梨树各器官对N肥的吸收征调能力,氮素损失少。     

Interspecific cross‐hybrids of Nicotiana tabacum L. cv. (gla.) S ‘K326’ with Nicotiana alata

In order to introduce the Tomato Spotted Wilt Virus (TSWV) resistance from Nicotiana alata into Nicotiana tabacum, a cytoplasmic male sterility (CMS) line of N. tabacum (N. tabacum L. cv. (gla.) S ‘K326’), was successfully crossed with N. alata. Despite a high DNA content variability, F₁ hybrids could be classified in two subgroups, a major one encompassing fertile hybrids morphologically similar to their tobacco maternal parent but TSWV sensitive, and a minor one displaying sterile hybrids showing an intermediate phenotype and TSWV resistant. In order to elucidate the unexpected fertility recovery of the fertile F₁ plants, some N. alata fertility restoration ppr genes were cloned and were shown to be differentially expressed between parental lineages as well as between both F₁ subgroups, suggesting that N. alata contains fertility restoring allele able to overcome the CMS of N. tabacum.     

Ecological implications of motor oil pollution: Earthworm survival and soil health

The nontarget effects of fresh and used motor oil were studied in a soil test system involving such criteria as earthworm survival, response of soil dehydrogenase and urease, and nitrification. When earthworms were exposed to motor oil-contaminated soil for 4 weeks, the observed median lethal concentrations (LC₅₀) were 40.33 and 3.88 g kg⁻¹ soil for fresh and used oil, respectively. Only fresh motor oil application increased earthworms' body weight even at the higher dose of 19 g kg⁻¹ soil. Gas chromatography/mass spectrometry revealed that used motor oil contained more of aromatic hydrocarbons and heavy metals than fresh oil. This disparity in the chemical composition might be the factor responsible for the significant toxicity of used motor oil towards earthworms. Activities of soil dehydrogenase and urease were significantly enhanced in presence of both the motor oils, while there was a significant inhibition in nitrification by the used motor oil even at a low concentration of 0.2 g kg⁻¹ soil. This study clearly demonstrated that earthworm survival and nitrification could serve as suitable indices to assess motor oil pollution in soil.     

青藏高原东北部3种野豌豆植物学特性及资源储量

采用野外定点法、最小样方法和统计学方法对生长于青藏高原东北部的3种野豌豆属植物的植物学特性、生态环境、群落数量特征及资源储量进行研究。结果显示,救荒野豌豆、山野豌豆和三齿萼野豌豆的植物学特性、生态环境和群落数量特征具有一定差异,3种野豌豆在青海省的资源总储量分别为0.46×10~6、0.23×10~6、0.27×10~6 kg,表明3种野豌豆属植物的资源总量在青海省并不高,应对其进行有效的引种驯化,实现人工繁育和增加资源储量,这既可保护青海省甚至青藏高原的生态环境,也可对其资源进行有效地保护和合理开发利用。     

Effects of forage type and gibberellic acid on nitrate leaching losses

Nitrogen (N) leaching from soil into water is a significant concern for intensively grazed forage‐based systems because it can cause a decline in water quality and is a risk to human health. Urine patches from grazing animals are the main source of this N. The objective of this study was to quantify the effect that forage type and gibberellic acid (GA) application had on N leaching and herbage N uptake from urine patches on perennial ryegrass–white clover (RGWC), Italian ryegrass and lucerne. A lysimeter study was conducted over 17 months to measure herbage growth, N uptake and N loss to water beneath each of the three forage types with the following treatments: control, urine (700 kg N/ha) and urine with GA (8 g GA active ingredient/ha). Compared with RGWC (205 kg N/ha), N leaching losses were 35.3% lower from Italian ryegrass (133 kg N/ha) and 98.5% higher from lucerne (407 kg N/ha). These differences in leaching loss are likely to be due to winter plant growth and N uptake. During the winter months, Italian ryegrass had higher N uptake, whereas lucerne had lower N uptake, compared with RGWC. The application of GA had no effect on N leaching losses, DM yield or N uptake of forage treated with 700 kg N/ha urine.