利用双杂合位点标记资料构建芒果遗传图谱

为了建立芒果 (MangiferaindicaL )的分子标记遗传图 ,用 15对AFLP (AmplifiedFragmentLengthPolymorphism)引物组合扩增了芒果品种间杂交组合 (Keitt×Tommy Atkins)的 6 0个F1单株 ,获得了 191个多态性位点。它们的分离表现为双杂合 (Aa×Aa)和测交 (Aa×aa)分离两种类型 ,但前者占了 5 9 7%。为了充分利用双杂合位点分离所提供的遗传信息 ,我们根据群体中任意两个双杂合位点隐性个体出现的数目 ,利用二项式分布概率理论推断它们是否连锁以及它们彼此间的相引或相斥关系。在该芒果群体呈 3:1分离的 81个多态性标记中 ,39个被分为 14组 ,以此为基础构建了 15个连锁群 ;这些连锁群共覆盖了 35 4 1cM的芒果基因组。其中 ,最小与最大遗传距离分别为 3 7cM和 2 8 9cM。此外 ,对 18个 1∶1分离类型的标记 ,直接利用Mapmaker作图软件构建了两个芒果连锁群。本文对所提出的利用双杂合位点构建果树遗传图谱的策略进行了讨论。     

Development of a crop knowledge base for Europe

This paper describes the development of a Crop Knowledge Base System for use in crop modelling and yield forecasting. The inherent limitations of databases can cause problems for dealing effectively with geo-referenced data sets. The system described here includes all the normal database operations but also handles richer types of data. Values of queried attributes can be deduced through the use of heritability within hierarchies and by the application of rules that summarise sets of empirical observations. The system is able to reason about similar locations, so that where no information exists in the knowledge base for a particular location, then an alternative location, subject to similar meteorological and agricultural conditions, will be sought.     

Effects of enhanced supplies of nitrogen and sulphur on rhizosphere and soil chemistry in a Norway spruce stand in SW Sweden

Rhizophere and bulk soil chemistry were investigated in a Norway spruce stand in SW Sweden. The rhizosphere and bulk soil chemistry in water extracts in control plots (C) and plots repeatedly treated with ammonium sulphate (NS) were compared. Treatment regime was started in 1988. Cylindrical core samples of the LFH-layer and mineral soil layers were collected in 1992 and used for water extract analyses. Samples of soil from LFH-layer and mineral soil layers were taken in 1991 and 1993 for determination of CEC and base saturation. Soil pH and NH₄-N, NO₃-N and SO₄-S, Al, Ca, K and Mg concentrations in water extracts were measured for rhizosphere and bulk soils. The pH-values of bulk and rhizosphere soils in NS plots decreased compared with those in control plots, whereas concentrations of NH₄-N, NO₃-N, SO₄-S, base cations and Al in water extract increased. In both bulk and rhizosphere soils the concentration of NH₄-N was much higher than that of NO₃-N. A significant difference in the pH and Mg concentration of bulk and rhizosphere soil between the treated and control plots was found only in the 0–10 cm layer. For all layers, there was a significant difference in NH₄-N concentrations in the bulk and rhizosphere soil between the NS treatment and control plots. Concentrations of exchangeable base cations and the base saturation level in the LFH-layer decreased in the NS plots. The concentration of extractable SO₄-S increased in the NS plots. The NS treatment enhanced the amount of litter in L-layer, owing to increases in needle biomass and litterfall but led to losses of base cations, mainly K and Mg, from LFH-layer. It was concluded that the NS treatment displaced cations from exchangeable sites in the LFH-layer leading to higher concentrations of these elements in both rhizosphere and bulk soil.     

基于遥感技术建立正镶白旗生态建设工程数据库

 内蒙古正镶白旗位于浑善达克沙地南缘地带,生态环境形势严峻,迫切需要利用现代化的科技和信息手段,服务于生态建设。为实现全旗的资源数据管理,满足生态建设和环境保护等工作的需要,并向当地的生产和科研部门提供详细的基础资料,探讨利用遥感技术来建立生态建设工程数据库的技术方法,以及数据库在白旗生态建设项目管理和动态监测中的应用。     

亚热带花岗岩地区土壤矿物风化过程中盐基离子的释放特征

矿物风化过程中盐基离子释放遵从一定的化学计量关系,这种化学计量关系一般只能通过模拟实验来获取。本研究采用pH 7.0的EDTA-乙酸铵溶液将土壤中的交换性盐基离子完全洗脱出来,然后用Batch方法模拟不同pH溶液淋溶洗脱盐基和未洗脱盐基土壤,旨在消除土壤中交换性盐基离子的影响后更为准确地判断土壤矿物风化的盐基离子释放特征。结果表明:未洗脱盐基土壤的淋出液pH由3.73±0.14逐渐上升到4.23±0.06,主要原因是淋溶液中有高浓度的NH_4~+;洗脱盐基土壤矿物风化后淋出液pH从7.39±0.02逐渐下降到5.39±0.17,主要是由于土壤中可风化矿物减少。土壤交换性盐基离子会改变盐基离子释放特征、释放总量:未洗脱盐基土壤经酸雨淋溶后,各盐基离子释放均呈现急速下降后逐渐平缓的趋势,洗脱盐基土壤矿物风化后,K~+及盐基离子释放总量呈波动上升趋势,且盐基离子释放总量比未洗脱盐基土壤低。土壤交换性盐基离子的存在还会改变淋出液中的盐基离子化学计量关系:未洗脱盐基土壤的K~+︰Ca~(2+)︰Mg~(2+)︰Na+化学计量关系为11︰13︰4︰1(当量比),而洗脱盐基土壤为7︰2︰2︰1。K~+是盐基离子中风化释放量最多的,大部分K~+来自于土壤中云母的风化。因此,只有利用洗脱盐基土壤的盐基离子释放量才能准确计算矿物风化速率并获得准确的化学计量关系。土壤矿物风化作用随着淋溶液酸度增大而增强,但模拟一年降雨量的情况下,p H 3.5、4.5和5.5三种不同p H溶液对矿物风化后盐基离子的释放在实验期间没有显著性影响,较长时间后的差异性有待观察。本研究表明,可以通过预洗脱盐基土壤然后模拟酸雨淋溶的方法,观察矿物风化特征,特别是盐基离子释放的化学计量特征。     

加强企业实训基地建设 为社会和企业培养人才

介绍了山东百圣源集团有限公司在做好校企联合,培养人才方面的工作经验。     

Assessment of sugar maple tree growth in relation to the partitioning of elements in xylem along a soil acidity gradient

Partitioning of elements in tree xylem is being increasingly studied, as it suggests that elements are potentially mobile within the xylem long after their uptake. A recent study revealed that only the most mobile xylem fraction (water-soluble) of base cations (calcium [Ca], magnesium [Mg], and potassium [K]) increased at higher soil acidity, while the two mobile fractions (water- and acid-soluble) of acidic metals—potentially phytotoxic aluminium (Al), cadmium (Cd) and manganese (Mn)—were significantly enhanced on very acid soils. The current paper presents an investigation of soil-wood chemistry relationships with basal area tree growth. It was hypothesized that the growth of sugar maple would be reduced by low base cation and high acidic metal concentrations in the xylem mobile fractions. Sugar maple trees (n = 55) from six watersheds in southern Quebec, Canada were analysed by sequential chemical extractions for the water-soluble, acid-soluble and residual fractions of base cations (Ca, K, Mg) and acidic metals (Al, Cd, Mn) in xylem. Generally, tree growth was positively correlated to concentrations of base cations in wood (ρ = 0.27-0.50) and soil (ρ = 0.41-0.67), and negatively correlated to concentrations of acidic metals in wood (ρ = −0.33 to −0.52) and soil (ρ = −0.67). However, these relations differed depending on the element fraction considered. Water- and acid-soluble xylem concentrations of base cations and Al were among the best predictors of growth trends (R² = 0.46-0.51). The relationship between acidic metals and tree growth is further discussed.     

建设绿色食品原料基地 助推宁都脐橙产业发展

宁都地处江西南部,气候温和,雨量充沛,是赣南脐橙的主产区,2008年经农业部绿色食品办公室、中国绿色食品发展中心批准为全国绿色食品原料(脐橙)标准化生产基地,通过3 a的创建与建设,全县脐橙基地按照绿色食品原料(脐橙)标准化进行生产,产量和效益大幅度增长,推动了宁都脐橙产业的发展,实现了农民增收、农业增效和农村经济发展。     

高寒山区横坡绿肥聚垄免耕的效果与技术关键

高寒山区横坡绿肥聚垄免耕的效果与技术关键李明祥（贵州省六盘水市水土保持办公室，５５３００１）粮食的短缺和寻求摆脱粮食生产困境的路子是困扰高寒贫困山区生态建设和经济发展的一个历史性问题，是当前和今后一段时期山区治理开发中所面临的一项重要任务。出路何在？...     

秦岭南坡青杄林、青杄—华山松—红桦混交林林地土壤特性研究

本文探讨了秦岭南坡青林及青—华山松7红桦混交林林地土壤的养分、化学性质及酶活性特点。结果表明：针阔混交林林地土壤酸性淋溶过程及物质迁移过程比青林地土壤弱；混交林明显地增加了土壤有机质及N素含量而降低了土壤有效P及有效K的含量；阳离子交换量及水解性总酸度青林地土壤比针阔混交林地高而盐基饱和度比针阔混交林地低，说明了在青林地土壤阳离子交换量中置换性酸所占比例较大而盐基离子所占比例较小；脲酶活性是针阔混交林地比青林地高而磷酸酶活性比青林地低，这与两种林地土壤中水解N及有效P含量高低是一致的。