The complete mitochondrial genome and molecular phylogeny of Lueyang black-bone chicken

ABSTRACT

1. The objectives of the current study were to investigate the mitochondrial genome and molecular phylogeny of Lueyang black-bone chicken, and provide molecule base to preserve and explore the specific chicken strain.

2. Based on sequencing and clustering, the complete mitochondrial DNA map and sequences of Lueyang black-bone chicken were revealed, and two phylogenetic trees of Lueyang black-bone chickens based on D-loop sequences and the mitochondrial genome were constructed.

3. The results showed that the complete mitochondrial genome of Lueyang black-bone chickens is 16,784bp in size, consisting of 22 transfer RNA genes, two ribosomal RNA genes, 13 protein-coding genes, and one non-coding control region. The base composition of the complete mtDNA sequence is 30.28% for A, 23.78% for T, 32.42% for C, 13.52% for G. Additionally, 10 haplotypes of D-loop sequences in 32 Lueyang black-bone chickens were detected, which were distributed into 4 clades (A, B, C and E).

4. It was concluded that genetic diversity is wide in Lueyang black-bone chickens, and this strain has multiple maternal origins from different regions in China and neighbouring regions.     

我国热区疫霉种的分布、疫病及其防治研究进展

综述了我国热带地区疫霉种类及其引起的植物病害,并总结了疫病的防治措施,最后对疫病防治的新策略进行了展望。     

毕节地区干草调制研究

中国南方喀斯特地区干草调制受限于诸多自然因素,客观上阻碍了该类地区草食畜牧业的发展,本研究在喀斯特典型地区贵州毕节撒拉溪示范区选取扁穗雀麦(Bromus cartharticus)、高羊茅(Festuca elata)、鸭茅(Dactylis glomerata)、菊苣(Cichorium intybus)、紫花苜蓿(Medicago sativa)、白三叶(Trifolium repens)、红三叶(T.pratense)、多年生黑麦(Lolium perenne)8种牧草进行田间自然晾晒,记录各时间段牧草的重量、空气温度和湿度,并对历年该地区气候进行浅析,重点分析近3年来4、5、6、7月份的最高温和最低温变化,通过对该地区8种主要栽培牧草的干燥过程和水分散失规律的研究,结合多年降水和气温变化规律,分析该地区调制干草的可行性,以期为喀斯特地区畜牧业的发展提供一些数据支撑。结果表明,1)4种禾本科牧草的干燥速率明显优于3种豆科牧草和菊苣;2)从外观评价角度出发,白三叶、红三叶、菊苣均不适合进行牧草晾晒;3)在选择的影响指标中,对干草晾晒的影响程度表现为晾晒时间空气湿度温度;4)综合分析各气候因子,在贵州毕节地区7月份最适合进行干草调制;5)干草调制具备可观的经济收益。     

黑龙江农垦齐齐哈尔管理局奶牛养殖小区饲养经营模式及效益的探析

对农垦齐齐哈尔管理局奶牛养殖小区的饲养经营模式及效益进行了调查,分析了奶牛小区建设给奶牛群带来的主要变化。通过分析不同模式奶牛小区生产经营状况,确定适合该地区奶牛业发展的奶牛小区经营模式。     

喀斯特山区2个鲫鱼群体基于转铁蛋白的遗传多样性研究

为了解喀斯特山区2个不同鲫鱼群体在蛋白质水平的遗传多样性及其遗传差异,运用聚丙烯酰胺凝胶电泳方法对喀斯特山区2个群体鲫鱼的血清转铁蛋白电泳表型及多样性进行研究。结果表明:群体克隆多样性指数表现为草海鲫鱼(0.32)﹥红鲫(0.25,对照)﹥普安鲫鱼(0.13);3个鲫鱼群体的13个克隆由9个不同的等位基因产生,等位基因的频率分别为Tf~a0.144 9、Tf~b0.202 9、Tf~c0.036 2、Tf~d0.058 0、Tf~e0.007 2、Tf~f0.239 1、Tf~g0.014 5、Tf~h0.275 4、Tf~i0.029 0;普安鲫鱼与草海鲫鱼和红鲫无共享的基因型;普安鲫鱼与草海鲫鱼之间的遗传相似性指数较高(0.714 3),遗传距离较小(0.285 7)。总体来看,草海鲫鱼的克隆多样性水平较高,其转铁蛋白多样性程度较普安鲫鱼丰富;2个群体之间存在较近的亲缘关系,未达到遗传距离种的分化标准。     

根区滴灌对干旱山地苹果树生长发育和结实的影响

根区滴灌是将雨水促渗管与传统滴灌相结合而组装集成的一项节水灌溉新技术。为了探讨根区滴灌在山地果园的应用效果,为山地果园的栽培管理提供依据,本文以陕北米脂县6 a生山地红富士苹果树为研究对象,以世界公认的最佳苹果优生区洛川县的降雨量和土壤贮水量为灌水依据,分析了不同灌溉方式对苹果树萌芽、坐果、枝叶生长、果实发育和水分利用效率的影响,以及山地果园土壤水分达到优生区标准后与优生区苹果树生长结果的差异。结果表明,采用根区滴灌能有效提高苹果树的萌芽率和坐果率,萌芽率比传统滴灌提高了2.14%~2.76%,坐果率比传统滴灌提高了0.80%~1.42%;能有效促进苹果树新稍和叶片的生长,新稍长度比传统滴灌提高了1.44%~4.94%,百叶鲜质量比传统滴灌提高了2.13%~4.16%;能显著促进苹果树果实的生长,单果重比传统滴灌增大了7.05~14.99 g,优果率比传统滴灌提高了9.4%~14.7%;能显著提高水分利用效率,灌水量比传统滴灌节约了8.09%,水分利用效率比传统滴灌提高了10.62%~15.30%。可见,根区滴灌技术在陕北黄土丘陵区山地苹果生产中具有广阔的应用效果,但是,单纯依靠人为限量补水,山地苹果还不能达到优生区的生产水平。     

阿克苏地区经济发展与建设用地集约利用耦合研究

以阿克苏地区的经济发展与建设用地集约利用的两个子系统为研究对象,基于二者与社会协同理论,同时运用熵值法、统计分析、系统评价和耦合模型等方法对其进行研究。建立阿克苏地区经济发展与建设用地耦合模型,然后对耦合关系进行探讨,揭示经济发展与建设用地集约利用的关系。研究结果表明,阿克苏地区经济发展和建设用地集约利用水平都较低;经济发展—建设用地集约利用系统中两子系统在反正切函数模型中的协调性越来越差;两子系统在经济和建设用地集约利用水平较低时高度耦合,阿克苏地区的经济水平较建设用地集约利用水平快速发展,建设用地集约水平急需改善。     

三岔子林区黄檗野生种群资源状况的调查与分析

以吉林省三岔子林业局不同林龄的林分为对象,调查与分析黄檗野生种群的资源状况,结果表明:单位面积活立木平均株数在幼龄林林分中占12.3%,在中龄林中占3.8%,在成过熟林中占2.6%,3个龄组林分的平均株数比例为6.2%;单位面积活立木平均蓄积在幼龄林林分中占7.3%,在中龄林中占2.4%,在成过熟林中占1.3%,3个龄组林分的平均蓄积比例为3.7%。     

Nutrient availability and corn growth in a poultry litter biochar‐amended loam soil in a greenhouse experiment

Nutrient‐rich biochar produced from animal wastes, such as poultry litter, may increase plant growth and nutrient uptake although the role of direct and indirect mechanisms, such as stimulation of the activity of mycorrhizal fungi and plant infection, remains unclear. The effects of poultry litter biochar in combination with fertilizer on mycorrhizal infection, soil nutrient availability and corn (Zea mays L.) growth were investigated by growing corn in a loam soil in a greenhouse with biochar (0, 5 and 10 Mg/ha) and nitrogen (N) and phosphorus (P) fertilizer (0, half and full rates). Biochar did not affect microbial biomass C or N, mycorrhizal infection, or alkaline phosphomonoesterase activities, but acid phosphomonoesterase activities, water‐soluble P, Mehlich‐3 Mg, plant height, aboveground and root biomass, and root diameter were greater with 10 Mg/ha than with no biochar. Root length, volume, root tips and surface area were greatest in the fully fertilized soil receiving 10 Mg/ha biochar compared to all other treatments. The 10 Mg/ha biochar application may have improved plant access to soil nutrients by promoting plant growth and root structural features, rather than by enhancing mycorrhizal infection rates.