CLINICAL,DIAGNOSTIC, AND PATHOLOGICAL FEATURES OF 2 CASES OF METASTATIC IRIDOPHOROMA IN A VEILED CHAMELEON (CHAMAELEO CALYPTRATUS) AND A RED-BARRED DRAGON (CTENOPHORUS VADNAPPA)

Iridophores are iridescent cutaneous pigment cells found in reptiles, fish, and amphibians. Neoplasms of iridophores are rarely reported, and little is known about their behaviour, metastatic potential, and prognostic indicators. This paper reports details of the clinical course and pathological findings of metastatic iridophoroma in a veiled chameleon (Chamaeleo calyptratus) and red-barred dragon (Ctenophorus vadnappa). The veiled chameleon presented with a subcutaneous mass on the right lateral elbow and was diagnosed as an iridophoroma on fine needle aspiration. It was otherwise clinically normal. Within 35 days of excision, multiple secondary skin masses developed which were often non-pigmented and required microscopic examination to identify them as iridophoroma metastases. There were in total 54 days between the first detection of the primary mass and end stage, extensive cutaneous and visceral metastatic disease, and there had been no detectable primary mass during routine clinical examination 128 days prior to death. The red-barred dragon presented with a white sub-mandibular mass. While undergoing an excisional biopsy the animal expired, post-mortem examination revealed a cutaneous malignant iridophoroma with tumour cell clusters seen in pulmonary arterial vessels, suggesting that surgical handling of iridophoroma could precipitate the release of circulating tumour cell clusters. This is the first iridophoroma described in a red-barred dragon and the second report of a malignant iridophoroma in a veiled chameleon. Timely detection and careful excision of lizard iridophoroma may be an important factor in clinical outcome.     

核桃和铁核桃品种（优系）叶形态构造与其抗旱性的研究

选取６项叶片旱生结构指标：角质层厚度,栅栏组织厚度／叶肉组织厚度,细胞密集度,主脉厚度和主脉两端厚角细胞层数,观察测定了１３个核桃和铁核桃品种（优系）的抗旱性。方差分析结果显示,１３个核桃和铁核桃品种（优系）在每个抗旱性指标上均差异极显著,故此对各品种（优系）进行了两两之间的单因素多重比较。依据前３个指标利用绝对法对各品种（优系）抗旱能力的大小进行了排序,其抗旱能力的次序为：新野,西洛２号,香玲,西洛６号,西洛３号,西扶２号,辽核１优,西林３号,辽核４号,辽核１号,西洛１号,西林２号,西扶１号,铁核桃（娘青夹绵）。     

苦丁茶的组织培养研究

诱导苦丁茶具节茎段芽体发生、生长与增殖的培养基,以改良ＡｎｄｅｒｓｏｎＭＳ培养基＋ＢＡ２．０ｍｇ／Ｌ＋ＮＡＡ０．１ｍｇ／Ｌ＋ＺＴ２．０ｍｇ／Ｌ＋ＧＡ３０．５ｍｇ／Ｌ较为适宜;以改良ＡｎｄｅｒｓｏｎＭＳ培养基＋ＢＡ３．０ｍｇ／Ｌ＋ＮＡＡ０．２５ｍｇ／Ｌ＋ＺＴ１．０ｍｇ／Ｌ继代培养。培养时温度为２８℃,光照度为１５００ｌｘ,光照时间为１４ｈ／ｄ;生根处理用１／４ＭＳ＋ＩＢＡ１．０ｍｇ／Ｌ培养基。     

Jatropha tanjorensis Ellis & Saroja, a natural interspecific hybrid occurring in Tamil Nadu, India

Jatropha tanjorensis Ellis & Saroja, a species found abundantly in Tanjore, Pudukottai, Trichirapalli and Ramnad districts of Tamil Nadu state, India and grown as a fence plant showed intermediacy in phenotypic characters of J. curcas L. and J. gossypifolia L. A detailed survey at its place of occurrence supplemented with data employed from cytological and peroxidase isozyme studies revealed that J. tanjorensis is a natural interspecific hybrid between these two species. Since there was no seed set, propagation through tissue culture using leaf discs was attempted.     

一株鹅源高致病力禽流感病毒分离株血凝素基因的分析

禽流感病毒（AIV）的表面结构蛋白血凝素（HA）在决定病毒致病力、受体结合特性、宿主范围等方面起着关键作用。本研究初步鉴定为高致病力毒株的AIV鹅体分离株A/Goose/Guangdong/1/96（H5N1）的HA基因进行了克隆并测序。结果表明,这一在无胰酶条件下可在单层鸡胚成纤维细胞培养物上形成蚀斑的毒株在其HA裂解位点附近有连续6个碱性氨基酸的插入物,从而揭示了其高致病力的分子基础。进一步的序列比较发现这一毒株与1997年香港流感病毒A/Hongkong/156/97（HK/97）的HA基因核苷酸和氨基酸同源率分别高达98.0%和98.2%,且其6个糖基化位点、2个受体结合位点及裂解位点碱性氨基酸插入物的序列均完全一致,提示该毒株与HK/97具有相似的生物学特性。     

管道轴向裂纹面内压作用下的J积分计算

在管道的制造、安装和运行期间,不可避免地会产生类似于裂纹的缺陷,轴向理解纹是最常见的裂纹。确定含轴中裂纹管道在各种应力状态下的应力强度因子和Ｊ积分,是评价管道完整性和使用寿命的重要组成部分。根据Ｋａｒｌｓｏｎ和Ｂａｃｋｌｕｎｄ推导的考虑裂纹承载的修正Ｊ积分的表达式,结合帕里斯公式和权函数方法,论述了管道轴向理解纹面在内压作用下的Ｊ积分计算方法,并与其它计算方法进行了比较。     

福建单竹属一新种

本文报道了产于中国福建南部的竹亚科一新种.它是单竹属的有节竹.     

四川猪2型链球菌病的PCR检测

2005年7月中下旬,四川贵阳等地人、猪陆续发生一种发病惠、死亡快、高度散发的疾病,从病死猪组织中分离出19株链球菌,设计合成3对引物,分别对分离菌株进行了猪链球菌16SrRNA、CPS2J、MRP基因片段扩增,有15株菌3个基因的扩增结果均为阳性,表明此次疲情的病原为猪2型链球菌。     

苦丁茶冬青粗多糖的分离表征及其抗化活性研究

水提醇沉法分离制备苦丁茶冬青粗多糖样品,对其进行初步分离表征(包括总糖、糖醛酸、蛋白质、氨基酸含量和红外光谱分析)和体外抗氧化活性(清除DPPH·自由基、·OH自由基清除能力和还原能力)研究.结果表明,苦丁茶冬青粗多糖中总糖含量为30.67％、糖醛酸含量为12.72％、蛋白质含量为9.35％;含有16种氨基酸成分,总含量为7.72％,其中含有7种人体必需氨基酸,占氨基酸总量的40.41％;红外光谱显示该粗多糖样品中含有α-吡喃糖环结构;此外,苦丁茶冬青粗多糖具有较强的体外抗氧化活性,且其抗氧化活性与多糖浓度之间存在良好的量效关系.     

苦丁茶冬青粗多糖的分离表征及其抗氧化活性研究

水提醇沉法分离制备苦丁茶冬青粗多糖样品,对其进行初步分离表征（包括总糖、糖醛酸、蛋白质、氨基酸含量和红外光谱分析）和体外抗氧化活性（清除DPPH·自由基、·OH自由基清除能力和还原能力）研究。结果表明,苦丁茶冬青粗多糖中总糖含量为30.67％、糖醛酸含量为12.72％、蛋白质含量为9.35％;含有16种氨基酸成分,总含量为7.72％,其中含有7种人体必需氨基酸,占氨基酸总量的40.41％;红外光谱显示该粗多糖样品中含有α-吡喃糖环结构;此外,苦丁茶冬青粗多糖具有较强的体外抗氧化活性,且其抗氧化活性与多糖浓度之间存在良好的量效关系。