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131.
Kleinschmidt S Meneses F Nolte I Hewicker-Trautwein M 《Veterinary immunology and immunopathology》2007,120(3-4):80-92
It has been suggested but not proven that hypersensitivity type I reactions are involved in the pathogenesis of canine inflammatory bowel disease (IBD). The main effector cells in type I hypersensitivity reactions are mast cells (MCs). Canine MCs, as human MCs, can be subdivided into three subtypes according to their content of mast cell-specific proteases: tryptase (MCT), chymase (MCC), or tryptase and chymase bearing MCs (MCTC). In this study, numbers and subsets of mast cells were investigated in biopsies from the gastrointestinal tract of dogs with histopathologically confirmed lymphocytic-plasmacytic enteritis (LPE) (n = 4), lymphocytic-plasmacytic colitis (LPC) (n = 1) and eosinophilic gastroenterocolitis (EGE) (n = 11). Paraffin sections of formalin-fixed samples from the stomach, small intestine (duodenum, jejunum, ileum) and colon were stained by using a metachromatic staining method (kresylecht-violet; KEV) and a combined enzyme histochemical and immunohistochemical technique for chymase and tryptase. Additionally, immunohistochemistry with antibodies against T cells (CD3), macrophages (myeloid/histiocyte antigen) and IgA, IgG and IgM bearing cells was conducted. Quantitative evaluation of mast cells and semiquantitative scoring of immunohistochemically stained cells were performed. Between the two histopathologically defined groups clear differences concerning mast cell numbers were detected. In most affected intestinal tissue locations of dogs with LPE/LPC a decrease in metachromatically (kresylecht-violet) stained granule-containing MCs and immunohistochemically stained MCT,C,TC was found. This reduction could be due to mast cell degranulation, a T helper cell 1 dominated reaction pattern or a “thinning out” due to increasing T cells, IgA and IgG bearing cells. Dogs with EGE displayed higher variability in mast cell numbers but most of the affected large and small intestinal locations had increased numbers of MCs. In these cases, T cells, IgA bearing cells and macrophages also increased. Increased numbers of MCs and eosinophils seen in the intestinal mucosa of dogs with EGE could indicate the presence of a type I hypersensitivity reaction (T helper cell 2 pattern) in response to dietary antigens. Changes in cell numbers occurred also in unaffected locations of dogs with LPE/LPC and EGE which showed reduced MCT,C,TC, increased KEV positive cells and partially increased leucocytes and macrophages. 相似文献
132.
Inness VL McCartney AL Khoo C Gross KL Gibson GR 《Journal of animal physiology and animal nutrition》2007,91(1-2):48-53
Inflammatory bowel disease (IBD) is a common cause of chronic large bowel diarrhoea in cats. Although the aetiology of IBD is unknown, an immune-mediated response to a luminal antigen is thought to be involved. As knowledge concerning the colonic microflora of cats is limited and requires further investigation, the purpose of this study was to determine the presence of specific bacterial groups in normal and IBD cats, and the potential role they play in the health of the host. Total bacterial populations, Bacteroides spp., Bifidobacterium spp., Clostridium histolyticum subgp., Lactobacillus-Enterococcus subgp. and Desulfovibrio spp. were enumerated in 34 healthy cats and 11 IBD cats using fluorescence in situ hybridisation. The study is one of the first to show the presence of Desulfovibrio in cats. Total bacteria, Bifidobacterium spp. and Bacteroides spp. counts were all significantly higher in healthy cats when compared with IBD cats, whereas Desulfovibrio spp. (producers of toxic sulphides) numbers were found to be significantly higher in colitic cats. The information obtained from this study suggests that modulation of bacterial flora by increasing bifidobacteria and decreasing Desulfovibrio spp. may be beneficial to cats with IBD. Dietary intervention may be an important aspect of their treatment. 相似文献
133.
鸡传染性法氏囊病BK912变异株疫苗安全及效力试验 总被引:1,自引:0,他引:1
使用3批IBD-BK912冻干疫苗对20日龄在隔离器中饲养的SPF鸡经2次点眼、口服各2000TCID50,干二免后14天用100BID501084A法氏囊囊毒攻毒,对照组100%发病,免疫的3个组100%保护,通过对囊指数的测定,疫苗安全,对囊无损伤。 相似文献
134.
不同IBD疫苗对SPF鸡的免疫抑制作用 总被引:1,自引:0,他引:1
用A、B、C、D4种IBD毒性型疫苗对6日龄SPF鸡进行免疫接种,根据免疫5d后法氏囊的病理变化、囊/体比、囊指数以及免疫12d后对ND疫苗的免疫应答情况等,综合评定这4种疫苗的免疫抑制作用。结果发现,雏鸡早期免疫IBD毒性型疫苗对法氏囊有明显的损害,其中对囊损伤最轻的是A苗,最严重的是B苗。这4种IBD疫苗均对新城疫疫苗免疫产生免疫抑制作用,其中A苗引起的最轻、最短暂,D苗次之,最严重的是B苗。 相似文献
135.
136.
鸡传染性囊病二价活疫苗攻击保护效果的观察 总被引:3,自引:1,他引:2
使用SPF雏鸡进行了鸡传染性囊病二价活疫苗攻击保护效果的观察。14日龄免疫2000TCID50剂量的二阶苗或BJ836苗价苗。免疫后14d(28日龄)分别用不同剂量(1:1,1:10,1:100)的标准I型强毒株CJ801株或标准变异株强毒1084A株进行攻击,攻击后5d剖杀观察。二价革免疫组能抵抗极高剂量(1:1)的CJ801株及高剂量(1:10)的1084A株的攻击,但不能抵抗极高剂量(1:1 相似文献
137.
将120只白来航雏鸡随机分成4个实验组和对照组,分别于第1、2、3和4周龄感染传染性法氏囊病病毒和生理盐水。在感染后第7d,外周血液中白细胞数、血糖和胰高血糖素含量明显低于对照组;在感染后第14d,上述指标虽有所升高,但仍低于同期对照组。表明IBDV对体内淋巴组织有明显损伤,胰高血糖素的变化及血糖的降低可能是由于胰腺实质细胞变性坏死所致。 相似文献
138.
传染性法氏囊病Vero细胞灭活疫苗的免疫试验表明,用0.5和0.8mL/只剂量分别肌肉接种。10~14日龄首免,8d后可检出病毒特异性抗体,14d抗体P/N值为3.8~5.6.病毒中和抗体滴度为1∶32~1∶64;21dP/N值为6.3~6.8,中和抗体滴度平均为1∶1024~1∶2048,此时血清抗体对鸡的保护率为85.7%.30~35日龄二免,14d后P/N值5.0~5.2,中和抗体滴度高达1∶2048~1∶8192,对鸡的保护率为100%.二免后112d(147日龄)抗体仍保持较高水平,P/N值为3.2~3.4.野外试验表明,这种疫苗安全、有效。在3000只蛋鸡群平行试验,灭活苗比活苗抗体水平高出0.2个光密度值,抗体持续期长。接种灭活苗的鸡群5个月内未发生此病。 相似文献
139.