Towards the development of a standardized method to monitor lakeshore hydromorphological restoration measures

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复合木霉制剂防治黄连根腐病及其机理研究

为评价复合木霉制剂对黄连根腐病的防治效果, 并揭示其防病机理, 为黄连根腐病专用微生物农药的研发奠定基础, 本试验将深绿木霉Trichoderma atroviride、长枝木霉T. longibrachiatum、钩状木霉T. hamatum、拟康宁木霉T. koningiopsis等4种木霉配制的复合制剂和尖镰孢Fusarium oxysporum以不同的方式分别施用于黄连, 统计根腐病发生情况, 检测黄连根部防御酶活性, 用高通量测序分析根际土壤真菌群落结构。结果表明, 复合木霉制剂对尖镰孢导致的根腐病具有明显预防效果;复合木霉制剂和尖镰孢分别接种黄连可提高SOD、POD、CAT、PAL、PPO等防御性酶活性, 产生诱导抗性;而它们先后接种黄连可产生强化效应, 从另一个途径提高植株抗病性。复合木霉制剂和尖镰孢都会降低真菌的数量、多样性, 和某些真菌的相对丰度, 而复合木霉制剂的抑菌作用更强烈, 尤其能明显抑制尖镰孢、Ilyonectria sp.等病原真菌的生长, 且能改善土壤真菌群落结构。木霉和尖镰孢都能在土壤中较长期定殖。可见, 复合木霉制剂可以预防尖镰孢导致的黄连根腐病, 防病机理包括诱导黄连植株产生抗性, 接种后再遭受病原菌侵染产生的强化效应, 优化土壤真菌群落结构, 抑制土壤中病原菌等, 且有效期较长。因此复合木霉制剂具有开发为微生物农药防治黄连根腐病的潜力。     

The dynamics of bacterial community in a polyculture aquaculture system of Penaeus chinensis,Rhopilema esculenta and Sinonovacula constricta

Polyculture with Chinese shrimp Penaeus chinensis, jellyfish Rhopilema esculenta and razor clam Sinonovacula constricta (S.J.R) is a common aquaculture system in Dandong, China. Investigation on the composition and function of microbial communities and their relationship with environmental factors in aquaculture systems have crucial implications. Therefore, the sequencing of 16S rRNA gene was applied to profile the bacterial community composition of water and sediment in S.J.R polyculture system in the present study. Proteobacteria was the dominant phylum in S.J.R polyculture ponds following with Actinobacteria and Bacteroidetes in water; in contrast, more diverse phyla were detected in sediments. Water temperature, pH and dissolved oxygen were determined the bacterial community composition in the S.J.R polyculture systems. Specially, Planktomarina, PS1 clade, Candidatus Aquiluna and Fibrobacteres, which can resist the phytoplankton blooms or degrade the cellulose, were more abundant in water at the early stage of cultivation in the S.J.R polyculture system. Moreover, the functions of bacterial communities in water were mainly associated with cell communication mediated, while the functions related to basal metabolism were enriched in sediments. The findings of this study allowed us to better understand the bacterial community in the S.J.R polyculture system and accumulated data for the improvement of cultural management.     

间隙灌溉和控释肥施用对稻田土壤产甲烷微生物的影响

间隙灌溉和控释肥施用影响稻田CH_4的产生和排放,然而其微生物机理尚不清楚。本研究通过采集稻季田间原位试验新鲜土样,采用核酸定量技术(qPCR)和末端限制性片段长度多态性(T-RFLP)技术,研究间隙灌溉和控释肥施用对稻田土壤产甲烷微生物群落丰度和结构的影响。结果表明,稻季CH_4排放量与古菌、产甲烷菌(mcr A基因)和甲烷氧化菌(pmo A基因)数量均呈极显著正相关关系(P0.01),而与细菌数量无显著相关性。间隙灌溉显著影响产甲烷菌和甲烷氧化菌数量的季节变化,其中烤田抑制产甲烷菌生长,而对甲烷氧化菌数量没有显著影响。与尿素相比,施用控释肥增加了稻田土壤细菌、古菌和产甲烷菌数量,降低了甲烷氧化菌数量。土壤古菌群落的优势T-RFs片段为184bp和391bp,其中184bp片段的相对丰度随着间隙灌溉的进行由45%~55%降低到23%~30%;而391bp片段则相反,其相对丰度由12%~18%增加到23%~26%。典型相关性分析(CCA)表明间隙灌溉显著影响土壤古菌群落结构(P0.001),而控释肥施用对土壤古菌群落结构没有明显影响。     

基于BERT-Attention-DenseBiGRU的农业问答社区问句相似度匹配

为了解决问答社区中相同语义问句文本的快速自动检测,提出一种基于BERT的Attention-DenseBiGRU农业问句相似度匹配模型.针对农业文本具备的特征,采用12层的中文BERT文本预训练模型对文本数据进行向量化处理,并与Word2Vec、Glove、TF-IDF方法进行对比分析,得出BERT方法能够有效地解决农...     

湛江市中澳友谊花园鸟类多样性研究

为了解湛江城市公园的鸟类动态与多样性,2017年7月至2018年6月,采用样点法及样线法,对湛江市中澳友谊花园鸟类群落组成及多样性进行调查,共记录到鸟类40种,隶属8目22科。其中雀形目（Passeriformes）鸟类最多,共12科21种。记录国家二级保护鸟类3种,广东省保护鸟类5种。优势种为白喉红臀鹎（Pycnonotus aurigaster）、池鹭（Ardeola bacchus）、家燕（Hirundo rustica）,分别占总数的13.20%、11.33%、11.58%。鸟类多样性指数（H）为3.00,均匀度指数（J）为0.81;鸟类多样性3月最高,种类共20种,占总数的50%;9月的种类最少,共10种,占总数的25%。     

The importance of the riparian ecotone and river hydraulics for sustainable basin-scale restoration scenarios

1. The effect of riparian ecotone functional complexity and stream hydraulics on an upland river ecosystem has been analysed. 2. The amount of nutrients retained by bottom sediment was lowest on a sandy substrate (range: 26–104 mg m⁻² P-PO₄) and highest in wetland bays (range: 558–5368 mg m⁻² P-PO₄). A stream bed covered by Berula erecta had about three times higher retentive nutrient capacity ( x̄ = 584 mg m⁻² day⁻¹) than did a sandy substrate( x̄ = 205 mg m⁻² day⁻¹). 3. The amount of allochthonous organic matter (CPOM) deposited on the stream bed decreased with current velocity. The trophic potential of CPOM, measured as total protein, was significantly correlated with the amount of deposited CPOM (r = 0.863; p<0.00001) and depended on stream order. 4. Both invertebrate and fish biomass in the upland river were significantly correlated with calcium/bicarbonate (benthos: r = 0.858; p<0.006; fish: r = 0.918; p<0.001). 5. Fish biomass, diversity and species richness were highest in pools, lower in riffles and lowest in the run/transition zone. 6. Macroinvertebrate biomass was highest at an intermediate riparian ecotone complexity with an adequate supply of organic matter and incident light. Fish biomass followed the same trend, being lowest in heavily shaded areas and in open channels without riparian vegetation (range: 1–4.5 g m⁻²), but highest in ecotones of intermediate complexity (range: 1.6–92.8 g m⁻²). The ‘cascading effect’ of invertebrate density depletion, which was inversely related to fish biomass, was observed seasonally. 7. The above results indicate that riparian ecotone structure and the heterogeneity of the stream channel may regulate biodiversity, productivity and nutrient retention in the fluvial corridor. These quantitative data help to create alternative scenarios for sustainable river basin management. © 1998 John Wiley & Sons, Ltd.     

川西北不同沙化程度草地土壤细菌群落特征

研究川西北不同沙化程度草地（未沙化草地、轻度沙化草地、中度沙化草地、重度沙化草地）土壤细菌多样性和群落结构特征,利用Illumina二代高通量测序技术MiSeq对土壤细菌的16 SrRNA V₃—V₄可变区进行测序,研究土壤细菌多样性、物种组成和丰富度,并结合土壤理养分探讨影响细菌群落结构的环境因素,对发挥土壤潜在肥力,了解土壤健康状况,实现该区植被的管理与可持续利用有着重要的意义。结果表明:（1）不同沙化草地土壤养分具有明显差异,依次表现为:随着沙化程度的增加,土壤pH值逐渐增加,而土壤有机碳、全氮、全钾、碱解氮和速效磷逐渐降低;（2）不同沙化程度草地土壤样品中共检测到细菌的32个门,65个纲,169个目,优势菌门为变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、酸杆菌门（Acidobacteria）、绿弯菌（Chloroflexi）、浮霉菌门（Planctomycetes）,主要的优势菌纲为放线杆菌纲（Actinobacteria）、α-变形菌纲（α-Proteobacteria）、酸杆菌纲（Acidobacteria）、β-变形菌纲（β-Proteobacteria）、浮霉菌纲（Planctomycetacia）,与沙化草地相比,未沙化草地优势菌主要是变形菌门（Proteobacteria）和放线菌门（Actinobacteria）;（3）随着沙化程度的增加,OUT数目、Chao指数、Ace指数、Shannon指数逐渐减小,其中不同沙化草地土壤细菌覆盖率和Simpson指数差异不显著（p > 0.05）;（4）冗余分析和Pearson相关性分析表明,土壤pH值、土壤有机碳（SOC）和全氮（TN）是土壤细菌群落结构和多样性的主要影响因子。     

水氮控制对荒漠草原中小型土壤动物群落的影响

【目的】了解水氮控制对荒漠草原中小型土壤动物的影响,进而从土壤动物角度解读全球变化对荒漠草原的生态效应。【方法】2014年植物生长季,以内蒙古自治区乌兰察布市四子王旗短花针茅荒漠草原为研究对象,设置对照(CK,自然降雨状态)、减雨(R)、增雨(W)、施氮(N)、减雨施氮(R+N)和增雨施氮(WN)6个处理,观测不同处理中小型土壤动物群落和土壤环境参数的变化。【结果】短花针茅荒漠草原共捕获中小型土壤动物333 079.5只/m~2,隶属于节肢动物门的4纲10目47科,主要为弹尾纲(Collembola)、蛛形纲(Arachnida)和昆虫纲(Insecta);优势类群为疣虫兆科(Neanoridae)和等节科(Isotomidae),优势类群和常见类群构成了研究样地中小型土壤动物群落的主体。增雨、增雨施氮有助于提高短花针茅荒漠草原中小型土壤动物个体密度,减雨、减雨施氮处理减少中小型土壤动物个体密度,且水氮交互作用对中小型土壤动物个体密度影响表现显著,而对类群数无显著影响。短花针茅荒漠草原中小型土壤动物具有明显表聚性,各处理下0~10cm土层个体密度和类群数显著高于10~20cm土层(P0.05)。【结论】在未来降雨增加和氮沉降的全球变化背景下,中小型土壤动物个体密度的变化可能会更大。     

Comparative study of the intestinal microbial community of wild and cultured kaluga sturgeon,Huso dauricus

The kaluga, Huso dauricus (Georgi 1775), is a large‐sized semi‐anadromous sturgeon native to the Amur River basin. Gut microbes play an important role in the growth and development of fish. Because wild samples of this species are difficult to obtain, very few studies have analysed the microbial community of the kaluga gut. Here, we used high‐throughput sequencing to determine differences in the intestinal microbial communities of wild and cultured kaluga, to lay the foundation for development of micro‐ecological preparations that might ultimately assist with conserving wild kaluga by benefitting sturgeon aquaculture. Two 2‐year‐old (wild) fish collected from the Heilongjiang River basin were size‐ and age‐matched with two 2‐year‐old (cultured) fish obtained from a breeding base in Yunnan Province, for molecular analysis of gut samples. In total, 483,008 16S rRNA sequences were obtained. The intestinal microbial diversity was greater in the wild kaluga than in the cultured fish. The dominant phyla in the gut community of wild kaluga were Proteobacteria (47.9%), Fusobacteria (30.5%) and Firmicutes (8.85%), whereas samples from the cultured kaluga were dominated by Fusobacteria (78%). This result may be attributed to differences in environmental conditions, particularly water quality and temperature, as well as diet between the wild and cultured animals. The findings provide basic data to assist further research and the development of feed as a part of artificial breeding technology.