Occurrence of Cryptosporidium and Giardia on beef farms and water sources within the vicinity of the farms on Prince Edward Island, Canada

The objectives of this study were to determine the prevalence and assemblages of Giardia and species of Cryptosporidium on beef farms in Prince Edward Island (PEI), Canada, including the water sources associated with the farms, and to determine risk factors for infection of cattle with these parasites. Twenty beef farms were selected based on the presence of surface water < 500 m from the barn. Prevalence was determined by direct immunofluorescence microscopy, while genotyping and species determination were performed by nested-PCR and DNA sequencing. Giardia was detected in 42% (95% CI: 38-46%) of fecal samples from 100% farms while Cryptosporidium was detected in 17% (95% CI: 14-19%) of fecal samples from 80% of farms. The most predominant Giardia assemblage isolated was the livestock specific assemblage E (89%). The zoonotic assemblages A and B were found in 4 and 7% of the Giardia isolates that were genotyped, respectively. The Giardia assemblages were detected equally between the cows and calves examined. Overall, the most common Cryptosporidium species detected in this study was Cryptosporidium andersoni (49%), predominantly found in cattle >6 mo of age, while most Cryptosporidium bovis and Cryptosporidium pestis (previously Cryptosporidium parvum ‘bovine genotype’) isolates were detected in calves ≤ 6 mo of age. All Cryptosporidium ryanae isolates (four) were found in calves. Giardia cysts and Cryptosporidium oocysts were detected in 14 and 93% of surface water samples of 14 farms, respectively. Cryptosporidium oocysts were detected in three (15%) ground water samples of 20 farms. One Cryptosporidium-positive water sample, which was the only surface water sample amenable to genotyping, contained C. parvum. The farm-level risk factors investigated in this study, age of animals and location of the farm, were not associated with the risk of infection in cattle with either Cryptosporidium spp. or Giardia duodenalis.We conclude that beef cattle are a potential reservoir of Cryptosporidium spp. and G. duodenalis that could contaminate source water. There is the possibility of further transmission to humans on PEI if the source water is not properly treated prior to consumption.     

基于原型观测与DEM的强风化花岗岩小流域水文过程模拟

为了解华南花岗岩小流域特殊的产流机制,以土壤为砂壤土、基岩为强风化花岗岩的中山大学珠海校区滨海小流域作为研究对象,观测了2个分别代表森林、灌丛覆盖的5 m×10 m的径流试验场的产流过程和土壤含水率变化过程。观测结果表明超渗产流、优先流是试验场的重要产流方式,壤中流（尤其是在土壤-基岩界面上产生的壤中流）对试验场的总产流量也有较大贡献。在径流试验场原型观测基础上,建立了一个基于数字高程模型（DEM）的、包括地表径流、壤中流和基岩裂隙出流的三水源小流域水文模型。利用9次降雨径流过程对模型参数进行率定,利用4次降雨径流过程进行验证,模型的率定、验证均取得了良好的拟合效果。根据模型的模拟结果,在径流的起涨阶段地表产流贡献最大,而基岩裂隙出流对退水过程贡献明显。综合试验场原型观测结果与模型模拟结果得出结论：明显的壤中流和基岩裂隙出流是华南花岗岩小流域显著的产流特点。     

3种地被植物在青岛市栽植应用试验与调查

佛甲草、德国景天、景天三七是3种优良的地被植物。笔者研究了3种地被植物的生长繁殖、栽植管理方式以及在青岛市园林中的应用状况,结果表明:在青岛3种地被植物扦插成活率较高,均能达到80%以上,夏季扦插效果最好;此外,抗寒性强,栽培管理粗放,使其适合以垂直绿化、屋顶绿化等方式应用于园林,在青岛市有待进一步推广。     

同安区雷电分布特征及天气类型关联性分析

[目的]利用闪电定位资料对厦门市同安区雷电活动规律进行深入研究。[方法]采用2005～2010年同安区雷电观测和闪电定位资料,分析同安区雷电分布特征。[结果]同安区雷电活动频繁,主要出现在3～11月,其中5～9月最为活跃;空间分布不均匀,北部山区多,南部平原少。对同期相关环流形势和天气系统进行统计,结果表明,产生雷闪的天气类型主要有高空槽类、副热带高压类和热带系统类等3类6型。[结论]该研究可为开展雷击损害评估、雷电灾害预警预报等工作提供参考。     

地沟装车双缆索减振系统受力分析

双缆索减振系统有效地减轻了下落原木材对运材车辆的冲击破坏，提高了汽车的使用寿命，使地沟装车得到广泛推广，推导出了冲击承载索后承载索产生的动态力，建立了承载冲击后的动荷系数公式，为设计双缆索减振系统奠定了理论基础。     

Responses of Climatic Change on the Lagging Time about Ground Temperature Reaching the Extremum at Shallow Layers Soil in Wuli

The air temperature of Wuli,which is located in seasonal frozen ground zone,is rising by 13 ℃ yearly.This paper discusses the days that each ground layers' temperature lags behind the surface temperature in reaching extremum.The results were shown:The time of each ground layers' lagging days was increasing;the lagging day in warm season was longer than that in cold season;the growth rate of lagging days in warm season was 0.5 d/y,while the growth rate of lagging days in cold season was 0.7 d/y.     

塑料大棚西芹多层覆盖安全越冬技术研究

在塑料大棚内，对西芹多层覆盖安全越冬技术进行了研究。结果表明：多层覆盖可明显提高冬季塑料大棚内的地温和气温，西芹收获早和效益高。该技术使大棚西芹冬季安全生产成为可能。     

利用探地雷达测定东北黑土层厚度在坡面的空间变化

准确掌握黑土层厚度分布信息对于黑土资源评价和保护具有重要意义。然而,传统土层厚度测定方法包括土壤剖面法、插钎法和钻孔法等对于大范围的土壤厚度测定效率较低且连续性较差。本研究利用探地雷达探测了东北黑土区直型、凸型和凹型3种坡型坡面的黑土层厚度。室内模拟试验对黑土及黄土母质土壤分别设置不同的容重和含水量,探究土壤含水量和容重对土壤介电常数的影响以及探地雷达测定黑土厚度的可行性。野外试验通过开挖剖面和预埋标识物,验证了探地雷达测量黑土厚度的准确性。结果表明：土壤介电常数随容重的增大而增大,随土壤含水量的增加而减小;黑土和黄土母质层土壤含水量、容重和介电常数之间的关系可以用两个对数方程来描述,其精确度为95.26%~99.66%。探地雷达测量黑土厚度与剖面实测厚度相比,精确度为87.05%~95.58%。3个坡面的黑土厚度空间分布不同,且坡脚发生沉积处的黑土厚度较大,坡肩和坡背土壤侵蚀较严重处的黑土厚度较薄。本研究可为进一步探明和保护黑土资源提供一种高效、准确的土壤厚度调查方法。     

Single and dual crop coefficients and water requirements for onion (Allium cepa L.) under semiarid conditions

The objectives of this study were to determine onion water requirements with a sprinkler irrigation system, the most usual irrigation method in Spain. A weighing lysimeter was used to measure single (Kc) and dual (Kcb + Ke) crop coefficient curves and obtain the relationship between Kc-ground cover (GC) and Kcb-GC. Experimental work was carried out in 2005 at “Las Tiesas” farm, located in Albacete (Central Spain). To determine actual onion evapotranspiration (ETc), we used a weighing lysimeter with continuous electronic data recording. Daily measured ETc values obtained by the lysimeter were compared to calculated ETc values obtained through the standard FAO methodology [Allen, R.G., Pereira, L.S., Raes, D., Smith, M., 1998. Crop evapotranspiration. Guidelines for computing crop water requirements. FAO Irrig. and Drain. Paper 56. Rome, Italy]. Seasonal evapotranspiration measured in the lysimeter (893.34 mm) was higher than the seasonal ETc calculated by FAO-56 method (832.90 mm). The percentage of GC was found through the supervised classification technique of digital photographic images with the maximum probability algorithm [Calera, A., Martínez, C., Melia, J., 2001. A procedure for obtaining green plant cover: relation to NDVI in a case study for barley. Int. J. Remote Sensing, 22, 3357-3362]. The values derived from lysimetric measurements are Kc ini: 0.65, Kc mid: 1.20 and Kc end: 0.75, similar to values given in FAO-56. Lysimetric measurements showed that the evaporative component was high during the growing season, due to the high frequency of irrigation and the fact that the onion crop does not completely cover the ground; maximum GC was 72%. Therefore, the dual crop coefficient was calculated, which allowed differentiation between crop transpiration (basal crop coefficient, Kcb) and evaporation from the soil (evaporation coefficient, Ke). With the aim of facilitating extrapolation of the results to other areas, Kc and Kcb were linearly correlated to fractional GC.     

几种野生地被植物高温半致死温度的确定

运用电导法对几种野生地被植物的抗热性进行了研究,叶片梯度高温处理后温度与细胞伤害率之间呈现“S”型曲线,通过显著性检验,复合Logistie方程。根据公式测得几种植物的半致死高温为:甘野菊53．95℃,蛇莓53．01℃,连钱草61．16℃,玉竹65．12℃,青杞48．59℃。