影响小麦赤霉病抗性的Lophopyrumelongatum染色体定位

禾谷镰刀菌 (FusariumgraminearumSchwabe)引起的小麦赤霉病穗腐是一种世界性病害。近几年 ,国际上许多研究者把赤霉病问题作为重点项目进行了研究 ,对赤霉病抗性遗传有了许多新的认识。本研究中 ,我们利用Lophopyrumelongatum染色体附加系为材料研究发现 ,Lophopyrumelongatum的赤霉病抗性在小麦背景中得到充分表达 ,其赤霉病抗性主要归功于 1Ee 染色体的作用 ,同时 3Ee,4Ee,6Ee 染色体可能有作用微弱的基因存在     

果树基因转移研究进展

综述了近年来果树基因转化的主要方法及在果树上的应用。果树基因转化方法主要包括农杆菌介导法、电激法、基因枪法、PEG转化法等。果树基因转化研究已在柑桔、苹果及香蕉等 2 0多个树种上取得了成功     

玉米优良自交系转基因受体系统建立及转化后的筛选与再生

通过对培养基和培养条件的筛选和改进 ,提高了诱导玉米优良自交系幼胚产生胚性愈伤组织 ,建立转基因受体系统的效率。通过比较试验 ,确定了潮霉素筛选的浓度和方法。PCR检测证明 ,目的基因已整合到玉米基因组中。     

用世代均数法对玉米农艺性状进行基因效应分析方法的研究

应用六个世代和八个世代的平均数对两个玉米杂交组合三合单交、成单四号进行基因效应分析,结果表明: 1、用加性、显性和双基因互作模型,能比较满意地解释玉米主要农艺性状的遗传。 2、用六个世代均数和八个世代均数的方法,对同一组合进行基因效应分析,所得结果是一致的。     

Reproductive biology of Corymbia citriodora subsp. variegata and effective pollination across its native range in Queensland,Australia

The spotted gum species complex represents a group of four eucalypt hardwood taxa that have a native range that spans the east coast of Australia, with a morphological cline from Victoria to northern Queensland. Of this group, Corymbia citriodora subsp. variegata (CCV) is widespread in south-eastern Queensland and northern New South Wales. It is currently the most commonly harvested native hardwood in Queensland. However, little basic knowledge of the reproductive biology of the species is available to inform genetic improvement and resource management programmes. Here we take an integrative approach, using both field and molecular data, to identify ecological factors important to mating patterns in native populations of CCV. Field observation of pollinator visitation and flowering phenology of 20 trees showed that foraging behaviour of pollinator guilds varies depending on flowering phenology and canopy structure. A positive effect of tree mean flowering effort was found on insect visitation, while bat visitation was predicted by tree height and by the number of trees simultaneously bearing flowers. Moreover, introduced honeybees were observed frequently, performing 73% of detected flower visits. Conversely, nectar-feeding birds and mammals were observed sporadically with lorikeets and honeyeaters each contributing to 11% of visits. Fruit bats, represented solely by the grey-headed flying fox, performed less than 2% of visits. Genotyping at six microsatellite markers in 301 seeds from 17 families sampled from four of Queensland's native forests showed that CCV displays a mixed-mating system that is mostly outcrossing (t _m = 0.899 ± 0.021). Preferential effective pollination from near-neighbours was detected by means of maximum-likelihood paternity analysis with up to 16% of reproduction events resulting from selfing. Forty to 48% of fertilising pollen was also carried from longer distance (>60 m). Marked differences in foraging behaviour and visitation frequency between observed pollinator guilds suggests that the observed dichotomy of effective pollen movement in spotted gums may be due to frequent visit from introduced honeybees favouring geitonogamy and sporadic visits from honeyeaters and fruit bats resulting in potential long-distance pollinations.     

猪RYR_1基因对生产性能的效应研究

采用ＰＣＲ－ ＲＦＬＰ 技术检测经选型交配的乐山长白猪子代群体的ＲＹＲ１ 基因型，并将其肥育，进行生产性能测定。通过分析ＲＹＲ１ 基因型与主要经济性状的相关性发现：在繁殖性状上，杂合子的Ｔ 基因未表现出明显效应；在生长性状上，ＲＹＲ１ 基因也未表现出明显效应；在胴体性状上突变基因Ｔ 有提高屠宰率和降低骨率的效应；在肉质性状上，Ｔ 基因具有增加肌肉失水率、降低肉色评分的效应。     

猪RYR1基因对生产性能的效应研究

采用PCR-RFLP技术检测经选型交配的乐山长白猪子代群体的RYR     

动物GH基因和前GH—级结构的比较研究

本文依据大量文献资料较系统的综合分析了动物GH基因和前GH的一级结构及其物种间存在的保守住和变异性。结果表明：朋基因有5或6个外显子，其cDNA长度一般为800－1200hp；前GH的信号肽和成熟肽分别为16－27和186—191个氨基酸。外显子的数目和大小在不同纲动物间存在较强的保守性。哺乳纲动物间和鸟纲动物间在基因长度、外显子数目和大小、内含子大小、碱基组成、5’端和3’端非编码序列、局部减基序列、前GH的氨基酸数目和序列等方面存在着很强的同源性，鱼纲动物间这种同源性表现较弱；同纲内分类地位越接近的物种其同源性越强；哺乳钢与鸟纲动物间有一定的保守性。动物GH基因和前GH的一级结构具有种的特异性，物种间在基因及其内含子的长度以及前GH的氨基酸数目上的变异似乎与物种的进化程度有关。     

长江流域水牛血清白蛋白多态性研究

本研究采用淀粉凝胶电泳法对我国长江流域五个沼泽型类群的395头水牛型清白蛋白多态性进行了检测。结果表明:其白蛋白受Alj~A、Alj~B、Alb~X三个等显著基因控制,基因频率分别为0.218、0.004、0.778,ALb~X为优势基因。表现为AA、AX、BX、和XX四种类型,基因型频率分别为0.074,0.028、0.008和0.630,XX为优势基因型。各水牛类群的基因及基因型频率间无显著性差异(P>0.05),呈现较高一致性,具有同源关系。     

楠竹的开花生理与经营管理的生理基础

本文论述了楠行的开花、施肥、种植密度、采伐、挖笋和栽培管理措施,为提高楠竹产量提供了理论依据。