温室环境多点数据嵌入式智能监测系统

应用温室技术进行农作物种植是实现我国农业现代化过程中的重要环节,温度和湿度是温室控制中的重要环境参数。为实现对多点温湿度数据的自动监测,设计了以32位ARM处理器S3C44B0X为核心的多路数据采集和处理系统。该系统采用单一采集中心和多个智能采集节点的分布式结构,节点与中心采用RS-485总线进行通信,采集中心实时地收集、处理和显示各智能节点传回的温湿度数据,可有效提高数据采集工作的效率和稳定性。     

利用基因芯片分析西南麦区主栽小麦品种川麦104的遗传构成

川麦104是西南麦区近年来选育的主栽小麦品种,为研究其高产、抗病遗传特性,利用3种小麦基因芯片(660K SNP、50K SNP和35K SNP)对川麦104及其双亲川麦42、川农16进行分析,探究川麦104的遗传构成。结果表明,在能定位于小麦不同染色体的SNP中,川麦104中与双亲相同的共有等位变异位点数目远多于其他类型位点,分别占定位位点总数的75.90%、74.21%和81.08%。川麦104中来源于双亲的SNP位点在染色体A、B和D基因组中分布不均匀;3种基因芯片扫描结果显示,在D基因组中来源于川麦42的遗传位点数均多于川农16。从21条染色体分布来看,川麦104中来源于川麦42的等位位点在7A、1B、2B、5B、1D和5D染色体上分布较多(遗传贡献率≥70%),其中在7A染色体上遗传贡献率超过90%;来源于川农16的等位位点在6A、3B和4B染色体上分布较多(遗传贡献率≥70%),其中在6A和4B染色体上遗传贡献率超过90%。功能基因分型结果表明,川麦104中绝大多数的优良等位基因均同时继承于双亲川麦42和川农16,在少部分重要性状上,川麦104还分别继承了双亲中的优良等位基因。这些优良基因正是川麦104在产量、抗性等各方面表现优良的分子基础。     

Effects of propofol on invasion and migration of human gastric cancer SGC-7901 cells

AIM: To investigate the effects of propofol on invasion and migration of gastric cancer cell line SGC-7901. METHODS: Cultured gastric cancer cell line SGC-7901 was randomly divided into 4 groups, and then diffe-rent concentrations (1, 3, 5 and 7 mg/L) of propofol were added and incubated for 24 h. The cell viability was measured by MTT assay. The invasion and migration abilities of the SGC-7901 cells were detected by Transwell assay and wound-healing assay. The expression of cysteine-rich angiogenic inducer 61 (CYR61), CD44v6 and matrix metalloproteinase-7 (MMP-7) in the SGC-7901 cells were examined by immunocytochemistry and Western blot.RESULTS: Propofol at 5 mg/L does not affect the viability of SGC-7901 cells, whereas significantly suppresses the invasion and migration abilities, and down-regulates the expression of CD44v6 and MMP-7 (P<0.05). CONCLUSION: The decreased invasion and migration abilities of SGC-7901 cells were partly due to the inhibition of CD44v6 and MMP-7 expression.     

水稻新品种新稻44号选育及超高产栽培技术

由新疆农业科学院核技术生物技术研究所、新疆农业科学院温宿水稻试验站、新疆金丰源种业股份有限公司联合选育的新稻44号,是以A稻6号为母本,以辽19-1为父本进行人工有性杂交后经系谱法选育而成。2年区域试验和1年生产试验平均产量分别为12 502.5kg/hm2和12 349.5kg/hm2,分别比对照秋田小町增产16.2%和12.73%。该品种2014年12月通过新疆维吾尔自治区农作物品种审定委员会(稻麦组)审定,其主要特点是超高产、抗病、抗倒伏。该品种适宜新疆中晚熟稻作区种植,特别是新疆阿克苏、喀什、和田三大稻区种植。     

44英寸热磨机结构特点

介绍了80000m3/a中密度生产线中所使用的44英寸热磨机的结构特点,详细阐述了进料螺旋、防反喷系统、卸料螺旋、带式螺旋、密封水系统、油润滑系统以及控制系统的结构特点,展望了该热磨机的应用前景。     

Chicken seminal fluid lacks CD9- and CD44-bearing extracellular vesicles

The avian seminal fluid (SF) is a protein-rich fluid, derived from the testis, the rudimentary epididymis and, finally, from the cloacal gland. The SF interacts with spermatozoa and the inner cell lining of the female genital tract, to modulate sperm functions and female immune responsiveness. Its complex proteome might either be free or linked to extracellular vesicles (EVs) as it is the case in mammals, where EVs depict the tetraspanin CD9; and where those EVs derived from the epididymis (epididymosomes) also present the receptor CD44. In the present study, sperm-free SF from Red Jungle Fowl, White Leghorn and an advanced intercross (AIL, 12th generation) were studied using flow cytometry of the membrane marker tetraspanin CD9, Western blotting of the membrane receptor CD44 and electron microscopy in non-enriched (whole SF) or enriched fractions obtained by precipitation using a commercial kit (Total Exosome Precipitation Solution). Neither CD9- nor CD44 could be detected, and the ultrastructure confirmed the relative absence of EVs, raising the possibility that avian SF interacts differently with the female genitalia as compared to the seminal plasma of mammals.     

穗重型小麦品种川麦42与穗数型品种川农16遗传差异的SSR标记分析

川麦42和川农16是四川省近年育成的两种不同类型高产小麦品种。本文选用250对微卫星(SSR)标记引物对两个品种在DNA分子水平上进行了遗传差异的研究。结果表明:250对引物均能扩出清晰地条带,其中129对引物的扩增产物存在多态性差异,占引物总数的51.6%;这为进一步利用川麦42 X川农16构建的重组自交系标记川麦42和川农16的一些重要基因奠定了基础。     

Effect of TRIM44 silencing on proliferation of hepatocellular carcinoma cells and its molecular mechanism

ATM: To investigate the effect of tripartite motif-containing protein 44 (TRIM44) on the proliferation of hepatocellular carcinoma (HCC) cells and to study the molecular mechanism. METHODS: The expression of TRIM44 at mRNA and protein levels in normal liver tissues, HCC tissues, adjacent nontumor liver tissues, immortalized hepatocytes and hepatoma cell lines was determined by RT-qPCR and Western blot, respectively. The silencing of TRIM44 was conducted by transfection of vector expressing shRNA targeting TRIM44 (shTRIM44) in the HCC cells, and the protein level of TRIM44 was measured by Western blot. The viability of the HCC cells was analyzed by MTS assay. The DNA synthesis of HCC cells was detected by Click-iT EdU Imaging Kit. The ability of anchorage-independent growth was determined by the method of colony formation on the soft agar. The effects of TRIM44 on the total protein and phosphorylation of mammalian target of rapamycin (mTOR) levels were measured by Western blot. The HCC cells were transfected with shTRIM44 and treated with mTOR agonist MHY1485, and the cell viability was analyzed by MTS assay. RESULTS: The mRNA and protein levels of TRIM44 in the HCC tissues were significantly higher than those in the adjacent nontumor liver tissues and normal liver tissues. In addition, the mRNA and protein levels of TRIM44 in the hepatoma cell lines were significantly higher than those in the immortalized hepatocytes. TRIM44 silencing significantly inhibited the viability of HCC cells and reduced the abilities of DNA synthesis and anchorage-independent growth of the HCC cells. TRIM44 silencing decreased the phosphorylation level of mTOR protein. MHY1485 significantly antagonized the inhibitory effect of TRIM44 silence to the viability of HCC cells. CONCLUSION: TRIM44 silencing inhibits the proliferation of HCC cells possibly through down-regulating the activity of mTOR.     

Expression of macrophage CD44 receptor in the course of experimental inflammatory response of bovine mammary gland induced by lipopolysaccharide and muramyl dipeptide

The aim of this study was to investigate development over time of the surface expression of CD44 on macrophages during an inflammatory response of bovine mammary gland. Intramammary instillation of muramyl dipeptide (MDP) and lipopolysaccharide (LPS) resulted in a significant increase in the total count of CD44+ non-vacuolised macrophages (_NMAC) after 24 h. During resolution of the inflammatory response, there was observed a gradual decrease in the total count CD44+ _NMAC. The lower total count and proportion of CD44 + vacuolised macrophages (_VMAC) was observed as the effect of MDP and LPS at 24 h after induction (P < 0.01). During resolution, the total count and proportion of CD44 + _VMAC increased. We have demonstrated CD44 receptor is expressed during the inflammatory response caused by LPS and MDP and the effect of these components on CD44 expression was particularly evident during initiation of the inflammatory response. High expression of CD44 in resolution of inflammatory response may relate to macrophages´ involvement in the processes leading to restitution of injured tissues.     

The role of CD44 adhesion factor in canine mammary carcinomas

CD44 is an adhesion molecule implicated in the progression of human breast cancer. The purpose of this study was to describe CD44 antigen expression in canine mammary carcinomas and to evaluate its prognostic significance in relation to other clinico-pathological variables.A reduction in CD44 expression was significantly related to variables such as tumour size and adherence to underlying tissues but was not related to tumour location or to ulceration of the overlying skin. Complex (grade I) and anaplastic (grade III) carcinomas exhibited more intense expression of this antigen than did some tubulopapillary and most solid carcinomas (grade II). Although reduced CD44 expression was associated with infiltrative growth and vascular invasion in solid carcinomas, intense expression was also observed in anaplastic tumours. Although overall these findings suggest a role for this adhesion factor in canine mammary tumour development and progression, the complexity and apparently paradoxical nature of some of the findings currently limit the use of this immunohistochemical marker as a prognostic indicator.