油茶湘林11号无性系嫁接苗的抗旱性研究

本文以普通油茶为对照,在人为控水模拟自然干旱的条件下,通过测定嫁接苗叶片的永久萎焉系数、叶片相对含水量、超氧化物歧化酶活性等抗旱指标,研究了陆川油茶、广宁红花油茶、香花油茶作砧木的湘林11号一年生嫁接苗的抗旱性。结果表明:香花油茶嫁接苗永久萎焉系数为1.34,显著低于普通油茶、陆川油茶和广宁红花油茶嫁接苗分别为1.63、1.58和1.69,表现出较强的抗旱性;通过主成分分析和隶属函数值综合评定,4个物种作砧木的湘林11号嫁接苗的抗旱性依次为香花油茶普通油茶陆川油茶广宁红花油茶,香花油茶作砧木明显提升了嫁接苗的抗旱性。     

影响牛奶trans11C18∶1和CLA合成的因素

乳脂肪受日粮因素的调控的可塑性大，CLA是乳脂肪中的一种微量不饱和脂肪酸，影响乳脂肪的物理和生物学特性。CLA最初发现于奶牛瘤胃，但牛奶中CLA主要通过乳腺组织Δ9去饱和酶作用于trans11C18∶1而内源合成。研究结果表明，日粮因素、动物个体间的差异和动物组织间SCD酶活性的差异是影响乳脂CLA合成的关键因素。作者针对影响trans11C18∶1和Δ9去饱和酶的因素对CLA合成调控因素作了简要论述。     

Increased microbial activity and nitrogen mineralization coupled to changes in microbial community structure in the rhizosphere of Bt corn

The interactions between plant roots and soil microorganisms are essential for the function and stability of ecosystems, primary agricultural production and plant health. Despite the importance of soil microbes the response of these microbes to large-scale cultivation of genetically modified (GM) crops is still poorly understood. This study evaluated the potential impact of two lines of transgenic Bt maize on rhizosphere microorganisms. A time-course field experiment was conducted over a period of two years in two fields in Guadalajara (Spain) with monthly sampling from April to September. Rhizosphere soil was collected from transgenic (TG) and unmodified (WT) maize plants from each field and sampling time for the analysis of several important functional and structural soil quality parameters. Total microbial activity, as determined by H³-Thymidine and C¹⁴-Leucine incorporation, was found to be higher in the rhizospheres of the transgenic plants. Similarly, differences in potential ammonification and nitrification were observed in the second year of the study. In contrast, bacterial and fungal microbial catabolic abilities, as determined by Biolog ECO and FF plate analyses, respectively, were more influenced by sampling time than the transgenic nature of the plants. Microbial community structure was also studied by bacterial and phylum-specific PCR-DGGE and PCR cloning approaches. In general, differences were again more pronounced between sampling times, as opposed to between TG versus WT plants, although marked differences were observed within the Betaproteobacteria between plant lines. For the first time it describes the presence of Iamiaceae family in soil, specifically to TG plant rhizosphere. To summarize, the study showed that some important properties of rhizopshere microbes may be impacted by Bt maize cultivation and highlighted the fact that such potential effects need to be viewed within the context of seasonal and spatial variability.     

苏云金芽胞杆菌杀虫晶体蛋白CryIA单克隆抗体的制备及在转Bt基因棉毒蛋白检测上的应用

用苏云金杆菌菌株HD-1和HD-73分别发酵培养制备了孢晶混合物，孢晶混合物经分离、纯化、电泳得到CryIA、CryIA(c)杀虫晶体蛋白。用CryIA和CryIA(c)分别免疫家兔制备了两种多抗。用CryIA免疫小鼠，经细胞杂交、融合、克隆后筛选出4个单克隆株系A4F5F11、B4E6C7、B4E6D8、B4F5G11。用CryIA(c)多抗包被，B4E6D8上清液作为夹心抗体成功地建立了双抗夹心 ELISA，并检测了中棉所30、NUCOTN33B蕾期叶片中毒蛋白的含量，结果分别为23．4ng·g-1 FW、24．7ng·g-1 FW。     

优质专用弱筋小麦靖麦11号选育及开发

 弱筯小麦新品种靖麦11号是通过人工杂交系统选育成的优质旱地小麦品种。该品种具有高产、优质、抗病、抗逆性强、适应性广等优良性状。连续两年在云南省地麦良种区域试验中居首位，增产显著。日前通过栽培研究，正采取合同预约生产、合同供种、以加工利用促进原料生产的技术路线推广和开发利用。     

辣椒新品种辽椒11号的选育

辽椒 11号是利用麻辣灯笼椒雄性不育两用系 AB0 1与甜椒 94 33自交系配制的一代杂交种。中早熟 ,生育期 10 3d左右 ,果实灯笼形 ,有辣味 ,果面少有皱褶。每 6 6 7m2平均产量 5 0 0 0 kg。抗病毒病和耐疫病 ,适于塑料大棚、露地及秋延晚栽培。已在辽宁、河北、山东等地推广。     

农杆菌介导法将Bt(cryⅠA)基因导入大豆的研究

构建含有Bt(cry Ⅰ A)基因的植物表达载体pCAMBIA3300-Bt,以大豆子叶节为受体,通过农杆菌介导法将Bt基因导人大豆品种黑农37中,获得转基因植株.并进行人豆的再生和遗传转化系统优化的研究,以获得较高的转化率.结果表明:在6-BA浓度为1.7 mg·L-1时,丛生芽分化率最高,确定该品种大豆在从生芽分化阶段的草铵膦筛选浓度为3.5 mg·L-1获得转化质粒pCAMBIA3300-Bt的转基因植株,其中T1代PCR阳性植株19株.采用real-time PCR的方法对T1代抗性植株进行Bt基因的转录水平的分析,初步证明Bt基因已整合到受体大豆的基因组内.     

转Bt基因抗虫棉黄萎病发生规律探讨

１９９６～１９９７年系统调查了转Ｂｔ基因抗虫棉在平作棉田和麦套棉田黄萎病发生消长情况,结果表明,转Ｂｔ基因抗虫棉黄萎病发生规律与对照中棉所１２存在较大差异。转Ｂｔ基因抗虫棉与对照品种相比,黄萎病发生前期,病株率低于对照品种,扩展缓慢,发病后期,扩展速度加快,病指超过对照品种,且全年隐症期短,在麦套棉田的发病高峰比对照品种推迟１０ｄ以上。从气象因子与病指和病株率的相关分析,温度与空气相对湿度对转Ｂｔ基因抗虫棉黄萎病指和病株率的相关系数绝对值均大于对照品种,且温度和空气相对湿度对转Ｂｔ基因抗虫棉黄萎病指的相关系数绝对值大于对其病株率的相关系数,日照时数与转Ｂｔ基因抗虫棉黄萎病指和病株率的相关系数均达极显著水平     

转Bt基因棉及其抗虫性研究与利用进展

本文综述了转Ｂｔ基因棉的培育及应用,对鳞翅目害虫的抗性及对非鳞翅目害虫和天敌的影响,以及害虫对转Ｂｔ基因棉抗性的预防和治理等方面的研究进展。并针对我国的具体情况,讨论了害虫对转Ｂｔ基因棉抗性的预防策略及转基因棉ＩＰＭ体系的关键技术组成。     

DPC处理对转Bt基因棉伤流中激素运输量的动态影响

DPC 处理增加了转 Bt 基因棉主要生育阶段根系伤流量和伤流中 CTKs(iP+iPA、Z+ZR、DHZ+DHZR),IAA 和 ABA 的运输量,调节了 GAs(GA3,GA4)的运输量。