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YUAN Su-xia LIU Yu-mei FANG Zhi-yuan YANG Li-mei ZHUANG Mu ZHANG Yang-yong SUN Pei-tian 《中国农业科学(英文版)》2009,8(8):939-946
The relationship between the ploidy level of microspore-derived plants and chloroplast number in stomatal guard cells was studied in cabbage, broccoli, and Chinese kale. In the experiment, distribution statistics analysis and t-test were used to perform statistical analysis on chloroplast number of different ploidy level in those stomatal guard cells mentioned above, and morphology identifying and chromosome counting were used to test accuracy of counting chloroplast number in stomatal guard cells. The chloroplast average number in stomatal guard cells was very similar among the different leaf positions on the same plant and among significantly among the different ploidy the different locations in the same stoma in the same variety. All the leaf, while the chloroplast number varied distributions of the chloroplast number in different ploidy stoma were normal distribution fitted. A correlation has been established between ploidy and chloroplast number in the stomatal guard cells. In every single stoma of microspore-derived plants, the chloroplast number for a haploid should not be more than 10, diploids 11 to 15, and polyploids more than 15. The accuracy of this method for identification of different ploidy plants was 93.93%. Furthermore, the accuracy of this method was reliable and did not vary with the plants growth conditions. Therefore, the chromosome ploidy of plants derived from microspore culture in cabbage, broccoli, and Chinese kale can be identified by simply counting the chloroplast number in stomatal guard cells. 相似文献
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CAO Bi-hao LEI Jian-jun XIANG Chen-bin CHEN Guo-ju ZENG Guo-ping David J.Oliver ZHAO Shuang LU Ling-ling 《中国农业科学(英文版)》2008,7(3):314-320
In order to understand the function of TuR2, a candidate disease-resistance gene was isolated from cabbage, we transformed it into mustard (Brassicajuncea L. Linshicaoyaozi) which was susceptible to TuMV through Agrobacterium tumefacine-mediated method. Transgenic plants were detected by Southern blotting and Northern blotting. Our results confirmed that the TuR2 gene had been integrated into the mustard genome, and it showed different expression levels among primary transplants (T0). The primary transplants (T0) and the first progenies of transgenic plants (T1) were inoculated with TuMV in a greenhouse. The transgenic plants had high TuMV-resistance, whereas the serious virus disease symptom was observed in CK (no transformation plants). The TuR2 gene in the first progenies of transgenic plants (T1) showed dominant monogenic inheritance. Compared with CK, the progenies containing TuR2 gene had stronger resistance to TuMV. The TuR2 gene which was isolated from cabbage had the function of TuMV-resistance. 相似文献
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A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant). Two RAPD markers were screened out in 200 random primers using:BSA(Bulked Segregant Analysis). Two RAPD markers, designated as AG13/2000 and U16/660, were 7.7 cM and 8.38 cM apart from the TuMVresistant gene, respectively. The two RAPD fragments were converted to SCAR markers. SCAR markers were confined in germplasm. 相似文献
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任俊 《农产品加工.学刊》2011,(12):25
德国弗赖堡大学发表新闻公报,该校研究人员最新发现,西兰花、卷心菜这类常见蔬菜可充当"肠道安全卫士"的角色。这是因为其中含有一种名为硫代葡萄糖苷的物质。研究人员先用一种不含任何植物成分、也无硫代葡萄糖苷的食物喂养实验鼠,结果实验鼠肠道免疫功能减弱。研究人员随后 相似文献
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<正>"一瓶矿泉水的钱就能买6棵卷心菜!"对于山东省济南市历城区唐王镇的菜农来讲,这无疑是一场"灾难"。"今年这卷心菜不光便宜还抽薹。头两天一斤才合8分、1毛。前一个星期,每斤一直就只卖到一毛左右,今天价格总算回升了点,好的能到三毛了,不过这价格还是比往年低。明天要是外地车来的少了,菜价可能还会再降下来。"据悉,整个唐王镇还有近三千亩卷心菜未售出。4月19日商务部公布数据显示:上周36个大中城市18 相似文献
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据专家介绍,菜粉蝶又名菜白蝶,其幼虫就是常见的菜青虫。青虫对含有芥子油的十字花科蔬菜有趋向性,特别喜欢产卵在叶背上,不排除菜农对其超量喷洒农药的可能。所以吃十字花科蔬菜前,一定要注意清洗。用清水冲洗至少3—6遍,然后泡入淡盐水中再冲洗一遍。对卷心菜,可先切开,放在清水中浸泡1—2小时,再用清水冲洗,以清除残附的农药。 相似文献
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