黄芪多糖和益生菌对海兰褐蛋鸡生产性能和免疫功能的影响

试验选用1日龄海蓝褐蛋雏鸡120只,分为4个组,每组30只,对照组饲喂基础日粮,试验组在基础日粮中分别添加益生素107cfu·kg-1、黄芪多糖300 mg·kg-1、益生素5×106cfu·kg-1+黄芪多糖150 mg·kg-1,试验周期为50 d。试验结果表明,益生素和黄芪多糖均能提高雏鸡的生长性能,但差异不显著(P>0.05);各试验组较对照组均能显著提高免疫器官指数、抗体滴度和血清溶菌酶含量(P<0.05),而以益生素+黄芪多糖组效果最好,较对照组能提高胸腺指数>25%,法氏囊指数>14%,溶菌酶含量提高35%,抗体效价则要提高1个滴度。     

灵芝子实体微粉碎工艺优化试验

利用微粉碎技术将灵芝子实体粉碎成微粉,提高多糖的溶出率。结果表明:含水率低于10%的灵芝子实体,进行6min微粉碎,粉碎效果达到最佳。     

河南产猫爪草的质量分析研究

为研究河南产猫爪草的质量,按照《中国药典》(2015年版)对河南产猫爪草进行来源鉴别、性状鉴别、横切面显微鉴别、薄层色谱鉴别,并增加粉末显微鉴别、粗多糖和多糖含量测定项目。结果表明:各新增项目简单、准确、可行、重现性好,可为河南产猫爪草质量评价及质量标准制定提供依据。     

一起小尾寒羊传染性胸膜肺炎的诊治

1发病情况2010年5月初,四平山门镇有多数农户饲养的小尾寒羊出现发烧、咳嗽、呼吸困难等主要症状,畜主试用安乃近、头孢、黄芪多糖等治疗,效果     

Study on Antiviral Effect of Polysaccharides from Lagenaria siceraria (Molina) Standl.on NDV in vitro

To research on polysaccharides from Lagenaria siceraria (Molina) Standl.against Newcastle disease virus (NDV).Total polysaccharide and four different solvent extractions of Lagenaria siceraria (Molina) Standl.were extracted by the methods of hot water extraction and ethanol precipition in this study.The content of polysaccharide was measured by phenol-sulfuric acid method and infrared spectroscopy method.The safe concentration and growth of chick embryo fibroblast (CEF) were assayed by MTT method,in order to facilitated the comparison under the same level,the safe concentration was united as 78.125 μg/mL.Under the safety range of concentration,detected the block-virus activity,anti-virus activity and virus-killing activity of polysaccharides through the ways of pre-adding polysaccharides,post-adding polysaccharides and adding polysaccharides with NDV.The results showed that the direct inactivation and propagation inhibition activity of total polysaccharide and four different solvent extractions were stronger than anti-absorption function.Anti-virus inhibition rate of 70%,80% gradient alcohol precipitation of polysaccharides from Lagenaria siceraria (Molina) Standl.(LSP₇₀,LSP₈₀) and total polysaccharide (LSP_t)were 40.41%,44.54% and 61.85%,virus-killing inhibition rate were 44.74%,58.76% and 59.38%.LSP₈₀ had the highest virus inhibition rate as 37.14% in the block-virus activity of those five polysaccharides.In summary,70%,80% gradient alcohol precipitation and total polysaccharide in polysaccharides possessed better activity and would be as the materials for further research.     

虫草参多糖提取工艺研究

研究探讨虫草参多糖的提取工艺,通过在单因素实验的基础上进行正交实验研究,实验结果表明:各因素对虫草参多糖提取率的影响从大到小依次为B>A>C,提取温度>料液比>提取时间.虫草参多糖提取工艺的最优组合条件为A2B2C3,即料液比1:30,提取温度60℃,提取时间4 h,在此条件下虫草参多糖提取率54.45%.     

蝉拟青霉多糖的纯化工艺

以多糖含量为指标,对除蛋白、脱色、透析纯化工艺参数进行优化,以期提高蝉拟青霉多糖的纯化工艺水平。结果显示:以木瓜蛋白酶提取液,Sevag法脱蛋白2次基本除蛋白完全;AB-8型大孔吸附树脂纯化效果最好,在p H为7、温度25℃、4mg/m L上样液浓度、2m L/min上样速度;透析时间为72h的纯化条件下,纯化效果最好。     

Establishment of Detection Methods for Dexamethasone Sodium Phosphate in Injections by UPLC-PDA

A detection method of dexamethasone sodium phosphate in houttuynia injection,bupleurum injection,vitamin C injection,lincomycin hydrochloride injection,and asragulus polysacharin injection was established by UPLC-PDA.The Waters ACQUITY UPLC^® BEH C18 column(2.1 mm×50 mm,1.7 μm) was used,the mobile phase consisted of 0.75% triethylamine phosphatic liquor/solution (pH 3.0)-methyl alcohol-acetonitrile(50:45:5),the flow rate was 0.3 mL/min,the column temperature had remained 25℃,photodiode array detector wavelength range was 190 to 400 nm,the recorded wavelength was 242 nm,the injection volume was 10 μL.The linear range of dexamethasone sodium phosphatel was 10.0 to 200.0 mg/L (R²=0.9999),the mean recovery and RSD for dexamethasone sodium phosphate in houttuynia injection,bupleurum injection,vitamin C injection,lincomycin hydrochloride injection and asragulus polysacharin injection were 97.54% and 1.14%,101.59% and 0.19%,100.92% and 0.92%,99.82% and 0.73%,100.08% and 0.62%,respectively.This method was accurate,easy and fast,it was suitable for detection of dexamethasone sodium phosphatein houttuynia injection and other four kinds of injections.     

仙草多糖提取工艺条件优化研究

采用水提法浸提仙草，以仙草多糖的提取率为指标，研究料液比、浸提温度、浸提时间及浸提次数4个因素对其提取率的影响，并从中挑取合适的因素水平进行正交试验及SPSS分析。结果表明：在这4个因素中，对提取率影响最大的因素是浸提温度，然后是浸提时间，再次是料液比，最后是浸提次数；在料液比1:60、温度100 ℃、浸提时间3 h的最优条件下，仙草多糖的提取率高达17.89 %，此时仙草多糖的含量为44.13 %。     

裂褶菌多糖的研究进展

裂褶菌多糖(Schizophyllan)是裂褶菌(Schizophyllum commune)的主要活性成分之一.综述了裂褶菌多糖结构、提取纯化工艺和生理活性功能等方面的研究进展.