5种弥散神经内分泌细胞在鸭腔上囊中的表达特征

应用免疫组化技术并结合图像分析软件研究了胃泌素、β-内啡肽、胰高血糖素、5-羟色胺、生长押素在鸭腔上囊中的表达特征.结果显示鸭腔上囊小结相关上皮细胞呈胃泌素强阳性反应;滤泡间粘膜上皮呈β-内啡肽中强度阳性反应;淋巴滤泡皮质部有少量的β-内啡肽、胃泌素、5-羟色胺阳性细胞,呈中、强度免疫反应;淋巴滤泡髓质有胃泌素强阳性细胞;生长抑素和胰高血糖素均呈阴性反应.结果说明,鸭腔上囊中存在β-内啡肤、胃泌素、5-羟色胺弥散神经内分泌细胞,它们在淋巴滤泡皮质部的表达特征有利于其通过内分泌和旁分泌方式调节B细胞的发育.     

Failure criterion for timber beams loaded in bending,compression and shear

Abstract

Based on the elastic–plastic strength calculation, necessary for precise data explanation, a derivation is given of the failure criterion for combined bending, compression and shear. This exact limit state criterion should replace the unacceptable unsafe criteria of Eurocode 5 (EN 1995-1-1:2004). It is shown that the principle used thus far, of limited “flow” in axial compression as a determining failure criterion, for example, predicting no influence of a size effect, does not hold. Instead, it is derived and confirmed by the data that bending tension failure is always determining, showing the existence of a size effect, and correction of the existing calculation method is therefore necessary. Because of the primary importance of the size effect for the strengths, also for combined bending–compression, a simple derivation of the size effect design equations is given and discussed in an appendix.     

Expression Patterns of the Serine Protease Gene AlSP 3 in Apolygus lucorum (Hemiptera: Miridae) at Different Temperatures

As an important digestive enzyme gene, the serine protease gene AlSP 3 plays a key role in Apolygus lucorum for digesting conventional cotton. To understand the effect of different temperatures on the expression level of AlSP 3, we analyzed the expression patterns of AlSP 3 in A. lucorum reared at different temperatures using real-time polymerase chain reaction. The results showed that at the normal temperature of 24 ℃, the expression of AlSP 3 in A .lucorum at different nymphal stages was similar. The expressions of AlSP 3 in the female adult at the pre-mating stage and the male adult at post-mating stage were 19.71- and 7.16-fold higher than their control at the 1^st nymphal stage, respectively, which were significantly higher than at any other stages. However, expression in the 4^th to 5^th A. lucorum at the nymphal stage increased with rising temperature in the range of 21～30 ℃. At 30 ℃, the expression of AlSP 3 in the 2nd to 5^th A. lucorum was the highest. The expression levels of AlSP 3 in adult A. lucorum reared at different temperatures during the new emergence and pre-mating stages were significantly higher than that of the control reared at 24 ℃ (P < 0.01), while the expression during the post-mating stage at 18℃ was higher than that at other temperatures. When incubated at extreme temperatures (4 ℃ and 40 ℃), the expression of AlSP 3 in female and male adult A. lucorum was significantly higher than that at 24 ℃ (P < 0.01). Taken together, our results showed that the external environment temperature and the internal stage of A. lucorum or its sex all had significant effects on the expression of AlSP 3 in A. lucorum (P < 0.01), and that there was some interactions between them. Therefore, AlSP 3 is very important for the female adult A. lucorum to obtain nutrition at the early stages and temperature is an important limiting factor for the expression of AlSP 3.     

强震作用下双层球面网壳结构非线性动力响应分析

针对大跨网壳结构的动力特性,提出了在强烈地震作用下双层球面网壳结构的非线性分析和结构动力稳定性判别方法.单元模型中考虑几何非线性和材料非线性的双重影响.分析时,以静力荷载作用下的受力状态作为时程分析的初始状态,采用B-R判断准则确定其临界荷载,通过加速度峰值-结构最大位移曲线来判别结构的动力稳定性,据此找出其动力失稳临界点及破坏规律.通过算例验证了本文方法的有效性.分析结果表明,双层球面网壳结构考虑双重非线性是必要的;一致缺陷模态对结构承载力的影响比振型模态缺陷的影响大.     

快速大量提取甜菜DNA的新方法

以甜菜幼嫩叶片为材料,通过冷冻真空干燥将材料变成干粉,利用CTAB法对DNA提取程序进行了研究,建立了一种简单、快速的甜菜DNA提取程序,既节省时间,又可获得高质量的DNA,可以满足SSR及SRAP扩增的需要。     

居民潜在国内出游力地区差异及对策研究

在总结了其他学者对潜在国内出游力研究的基础上,运用因子分析的方法选取了11个指标对中国各个地区居民潜在国内出游力进行了分析,得出了4个综合因子;并用聚类分析法将中国居民潜在出游力划分成4大类。最后根据因子分析和聚类分析的结果,对中国居民潜在国内出游力的地区差异进行综合分析,提出了政策和营销方面的建议。     

2006-2007年小麦新品系选拔试验

通过对36份小麦品系比较试验,调查了小麦的株高、有效穗数、成熟期、穗长、结实小穗、实粒数、单穗重、千粒重等性状,并进行了聚类分析.结果表明:36份品系可分成5种类型,第1种为粒少、低产类型;第2种为穗少、低产类型;第3种为粒多、穗多、高产类型;第4种为穗多、粒多、粒大、高产类型;第5种为晚熟类型.     

悬索斜拉索组合式跨越管桥风致振动分析

应用大型有限元分析软件ANSYS,分别建立了大跨度悬索斜拉索组合式管桥建造时成桥状态和服役20年后运行状态的有限元模型。通过对管桥两种状况下的模态分析,获得管桥前二十阶固有频率和模态振型。在模态分析的基础上,进一步对管桥进行风致振动分析。分析结果表明,风力的等级对管桥疲劳载荷幅值的大小影响不大;管桥在服役20年后,管道和拉索的疲劳载荷幅值低于管道和拉索允许的疲劳强度值,不会引起管桥的疲劳失效,但要高于20年前建造时的疲劳载荷幅值水平。     

激光光致发光光谱分析系统的设计及应用

在微区紫外-可见阴极荧光分析系统成功研制的基础上,采用532nm固体激光器作为激发源研制了一套计算机自动控制的微区光致发光分析系统。该系统不仅可探测可见波段的激光光致荧光,并且将荧光的研究波段扩展至红外区域。对来自于不同地区金刚石样品的900～1010nm波段红外光致发光光谱进行探测,结果发现不同样品在946nm、957nm左右2个宽峰处呈现不同光谱特征,能有效地对样品的生长条件和受外界影响等因素进行分析和研究。该分析系统能在更宽的波段上获取更多更新的样品内在信息,为样品的测试提供了一种有效方法。