Global and Phylogenetic Distribution of Quorum Sensing Signals,Acyl Homoserine Lactones,in the Family of Vibrionaceae

Bacterial quorum sensing (QS) and the corresponding signals, acyl homoserine lactones (AHLs), were first described for a luminescent Vibrio species. Since then, detailed knowledge has been gained on the functional level of QS; however, the abundance of AHLs in the family of Vibrionaceae in the environment has remained unclear. Three hundred and one Vibrionaceae strains were collected on a global research cruise and the prevalence and profile of AHL signals in this global collection were determined. AHLs were detected in 32 of the 301 strains using Agrobacterium tumefaciens and Chromobacterium violaceum reporter strains. Ethyl acetate extracts of the cultures were analysed by ultra-high performance liquid chromatography-high resolution mass spectrometry (MS) with automated tandem MS confirmation for AHLs. N-(3-hydroxy-hexanoyl) (OH-C6) and N-(3-hydroxy-decanoyl) (OH-C10) homoserine lactones were the most common AHLs found in 17 and 12 strains, respectively. Several strains produced a diversity of different AHLs, including N-heptanoyl (C7) HL. AHL-producing Vibrionaceae were found in polar, temperate and tropical waters. The AHL profiles correlated with strain phylogeny based on gene sequence homology, however not with geographical location. In conclusion, a wide range of AHL signals are produced by a number of clades in the Vibrionaceae family and these results will allow future investigations of inter- and intra-species interactions within this cosmopolitan family of marine bacteria.     

Haloperoxidase Mediated Quorum Quenching by Nitzschia cf pellucida: Study of the Metabolization of N-Acyl Homoserine Lactones by a Benthic Diatom

Diatoms are known to produce a variety of halogenated compounds, which were recently shown to have a role in allelopathic interactions between competing species. The production of these compounds is linked to haloperoxidase activity. This research, has shown that this system may also be involved in diatom-bacteria interactions via the H₂O₂ dependent inactivation of a type of quorum sensing (QS) molecule, i.e., N-β-ketoacylated homoserine lactones (AHLs), by a natural haloperoxidase system from the benthic diatom Nitzschia cf pellucida. The AHL degradation pathway towards corresponding halogenated derivatives was elucidated via HPLC-MS analysis and the synthesis of a broad series of novel halogenated AHL analogues as reference compounds. Furthermore, their biological activity as quorum sensing modulators was directly compared and evaluated against a series of naturally occurring β-keto-AHLs. It has been demonstrated that the loss of the QS activity results from the final cleavage of the halogenated N-acyl chain of the signal molecules.     

Quorum Quenching Agents: Resources for Antivirulence Therapy

The continuing emergence of antibiotic-resistant pathogens is a concern to human health and highlights the urgent need for the development of alternative therapeutic strategies. Quorum sensing (QS) regulates virulence in many bacterial pathogens, and thus, is a promising target for antivirulence therapy which may inhibit virulence instead of cell growth and division. This means that there is little selective pressure for the evolution of resistance. Many natural quorum quenching (QQ) agents have been identified. Moreover, it has been shown that many microorganisms are capable of producing small molecular QS inhibitors and/or macromolecular QQ enzymes, which could be regarded as a strategy for bacteria to gain benefits in competitive environments. More than 30 species of marine QQ bacteria have been identified thus far, but only a few of them have been intensively studied. Recent studies indicate that an enormous number of QQ microorganisms are undiscovered in the highly diverse marine environments, and these marine microorganism-derived QQ agents may be valuable resources for antivirulence therapy.     

Multi-Temporal Detection of Rice Phenological Stages Using Canopy Spectrum

Information on rice phenology is essential for yield estimation and crop management. To test the ability of remote sensing in detecting multiple phenological stages, paddy rice canopy spectrum was measured by a hand-held radiometer. Normalized difference vegetation index(NDVI) was calculated from spectrum, and the slope of NDVI was obtained as its difference. We evaluated the response of NDVI and its slope to rice growth with a comparison of two late-season rice cultivars. The results showed that NDVI and its slope curves had distinct variation corresponding to rice development and they could be used as cultivar-independent phenological indicators. The dates of flooding and transplanting, tillering, panicle development, heading and flowering, maturity, harvest stages, and even field management practices, could be deduced from these indicators. ‘NDVI ≤ 0' could be used as a single threshold for the detection of flooding and transplanting. The largest spike in the curve of the NDVI slope indicated the duration of tillering stage. The next spike corresponded to panicle development. The heading and flowering stage was characterized by the maximum NDVI and the change of NDVI slope from positive to negative. At the maturity stage, NDVI decreased continuously, and its slope fluctuated just below zero. When rice grains were completely mature and ready for harvest, NDVI decline was accelerated. At harvest, NDVI slope reached its minimum value. The distinction between heading and maturity stages was obscure, most likely due to NDVI saturation at high biomass. The study might provide references for paddy rice phenology determination through remote sensing images.     

基于AHP-熵权TOPSIS模型的辽宁省各城市土地承载力评价

[目的]定量分析评价辽宁省各城市土地承载力状况并对其障碍因子进行诊断,为辽宁省土地利用合理开发提供建议,也为区域土地承载力评价研究提供新思路。[方法]以辽宁省14个地级市作为研究区域,基于2018年Landsat 8 OLI遥感影像,进行土地利用分类获得土地各类型用地面积并结合社会经济数据,构建了土地承载力评价指标体系,并利用AHP-熵权TOPSIS模型,对土地资源承载力进行评价。运用障碍度模型对土地承载力障碍因子进行诊断。[结果]辽宁省城市分别呈现不同的承载力状态。盘锦市、大连市以及沈阳市土地承载力相对较高,但社会子系统是制约其土地承载力的主要障碍因素。鞍山市和营口市承载力表现为相对低的状态,其经济子系统的是制约土地承载力提高的主要因素。[结论]辽宁省各城市土地承载力距离其理想状态还有着一定距离,应促进社会、经济、资源3个子系统协调发展进而提高土地承载力。     

基于高分六号卫星红边波段的森林蓄积量遥感反演——以西宁市针叶林为例

红光波段和近红外波段常被作为森林蓄积量反演的敏感波段,介于两者之间的红边波段往往被忽略,为了明确红边波段对森林蓄积量的遥感反演精度的影响,本研究基于高分六号卫星宽幅影像（GF6-WFV）,结合西宁市2014年森林资源二类调查数据,从光谱特征、植被指数、地形因子、影像纹理等4个方面选取蓄积量反演的自变量,采用多元线性回归（MLR）、随机森林（RF）模型,分析比较了有无红边波段对西宁市针叶林蓄积量遥感反演精度的影响。结果表明：（1）无红边波段（No Red-Edge）和加入红边波段（Red-Edge Added）两组处理的纹理特征变量降维后,其主成分信息主要解释了红外、近红外以及红边1波段的纹理特征。（2）与蓄积量相关的光谱特征变量：No Red-Edge处理主要包括红边波段和近红外波段的地表反射率,Red-Edge Added处理主要为红边1波段的地表反射率;植被指数变量：No Red-Edge处理主要包括NDVI和SAVI,Red-Edge Added处理为MTCI。（3）加入红边波段后,RF模型的R²优于MLR模型,分别为0.6719和0.5487,RF模型的均方根误差（RMSE）小于MLR模型,分别为26.3m³hm⁻²和20.8m³hm⁻²。（4）去除模型对反演精度的影响后,相对于No Red-Edge,Red-Edge Added处理的反演结果与观测值拟合的R2提高了11.6%,RMSE降低了9.1%。说明加入红边波段可显著提高西宁市针叶林蓄积量的反演精度,研究结果可为森林蓄积量的遥感反演提供科学依据。     

作物模型应用与遥感信息集成技术研究进展

作物模型和遥感技术以各自独有的优势在作物生产监测、评估及未来预测等方面发挥着关键作用。作物模型与遥感信息集成技术在大尺度、高精准的农业生产监测、评估与预测上具有明显的应用优势和广阔的发展前景。为了促进这些技术在区域尺度上的作物产量预测、农业气象灾害影响评估及农业应对气候变化决策等方面更加广泛地应用,本文采用文献综述的方法,系统归纳了欧洲、美国、澳大利亚及中国作物模型的发展与应用,总结了当前主流的数据集成方法的原理、特点和不足,概述了作物模型与遥感信息集成技术的实际应用,探讨了提升数据集成精度存在的问题,并对未来研究方向进行展望。结果表明,国内外对于作物模型及其与遥感数据集成的研究与应用广泛而深入,利用同化方法能够有效提高作物模型模拟精度,为作物模型实现区域尺度作物生长及产量评估、气候变化对产量影响、农田管理决策等提供技术支撑。作物模型模拟结果及遥感反演数据的不确定性、数据同化策略的多样性以及尺度效应是进一步提高集成系统精度与效率的限制因素。因此,遥感数据多源融合、同化过程多变量协同、作物模型多类型耦合以及数据高性能并行计算是未来作物模型与遥感数据集成研究的发展趋势。     

基于地理探测器的重庆坡耕地时空格局演变特征及驱动机制

坡耕地是耕地的重要组成部分,分析坡耕地时空格局演变规律及驱动因素,有益于为合理利用坡耕地资源及生态功能修复与保持提供参考。基于2000-2018年坡耕地空间分布信息,利用土地利用转移矩阵、局部空间自相关（Local Spatial Autocorrelation, LISA）、地理探测器（Geodetector）等方法,从坡耕地时空变化特征、坡耕地冷热点格局和坡耕地变化驱动因子等方面开展,揭示重庆坡耕地时空分布特征及驱动因素。结果表明：1）与2000年相比,2018年重庆坡耕地面积减少2.40%,坡耕地2.23 万km2,占耕地59.35%。6个区县（渝中、江北、江津、南岸、大渡口、石柱）年均坡耕地变化动态度大于1%,不同区县坡耕地面积变化趋势差异明显。2）近18年坡耕地与林地、草地、水田、建设用地等地类发生显著转移,转出、转入总体较均衡,其中坡耕地退为林地主要集中位于秦巴和武陵山区的石柱、武隆、丰都、云阳等区县,在土地利用转移过程中坡耕地面积呈小幅度减少趋势。3）坡耕地局部空间自相关异质性较显著,大多数区域坡耕地面积呈高-高和低-低聚集状态,热点区集中在渝东北区,冷点区分布在渝西区和主城区。4）林业产值、农村居民人均纯收入、粮食产量、乡村从业人员、农林水支出等经济、社会及政策因素是主要驱动因子,各因子间的交互作用大部分为非线性增强和双因子增强,其中解释力较大的交互驱动因子为粮食产量/坡度（2005年,0.927）、农村居民人均纯收入/常住人口（2018年,0.910）,海拔、降雨等自然因素对坡耕地变化有一定影响,但经济发展、政策调控、农民收入、城市扩张等社会经济因素是坡耕地变化的主要驱动因子。研究结果可为重庆以及西南山地丘陵区坡耕地资源保护与格局优化提供科学依据。     

2000-2020年玛纳斯河流域的作物种植结构与需水满足度

变化环境条件下干旱绿洲水土资源的高效利用一直广受关注。玛纳斯河流域分布着新疆最大、最典型的绿洲农耕区,其水土资源高效利用无疑应基于对种植结构和需水满足度（供水量/需水量）时空演变规律的了解。基于谷歌地球引擎（Google Earth Engine,GEE）平台并通过区域调查和调研,该研究利用面向对象的随机森林分类,建立流域地物遥感识别模型,分析当地2000-2020年种植结构的变化过程,探讨种植结构变化与膜下滴灌棉花水分供应状况和需水满足度间的关系。结果表明：基于GEE平台,融合简单非迭代聚类图像分割算法和随机森林分类算法可快速、准确识别流域地物,总体精度约90%;近20年,流域种植作物始终以棉花为主,占耕地总面积的80%以上,受益于膜下滴灌技术的节水、抑盐等功效,中、下游盐碱荒地不断被开垦为棉田,致使其面积以每年约101 km2的速度增长,但棉田面积增长与灌溉水资源供给的矛盾日益突出,棉花需水满足度显著下降,尤其是水资源相对匮乏的下游灌区及需水旺盛的夏灌期,2020年流域下游棉花夏灌期需水满足度已降至约46%,种植结构的调整和优化已势在必行。研究可为玛纳斯河流域水资源配置及农业种植结构优化提供科学依据。     

北京植被净初级生产力对物候变化的响应

植被净初级生产力（Net Primary Productivity,NPP）对物候的响应是全球气候变化背景下的重要研究内容,气候变化对植物物候与NPP的影响仍需明了,物候的时空变异规律更需深入探讨。该研究基于2001-2020年MODIS归一化植被指数（Normalized Difference Vegetation Index,NDVI）数据提取北京植被物候信息,利用CASA（Carnegie-Ames-Stanford-Approach）模型模拟NPP,通过线性回归、趋势分析和结构方程模型等方法,阐明NPP与物候时空变化特征,探究气象因素和物候变化对NPP的影响。结果显示：1）2001-2020年超过70 %的区域出现生长季开始（Start of Growing Season,SOS）逐渐提前,平均每年提前0.53 d。超过90%的区域生长结束期（End of Growing Season,EOS）逐渐推迟,平均每年推迟0.51 d。2）SOS提前和生长季（Length of Growing Season,LOS）延长均对NPP增长产生显著影响（P<0.05）。SOS每提前1 d,NPP增长3.74 g/m2;LOS每延长1 d,NPP增长2.56 g/m2 。秋季EOS推迟对NPP变化影响不显著。3）春季和秋季,气候通过改变物候（SOS、EOS）对NPP的间接影响大于直接影响,而夏季温度和降雨对NPP的直接影响更大。该研究表明春季物候变化是NPP年际变异的重要驱动因子,春季物候提前导致NPP年总量增加。研究结果是都市区植被生产力如何响应气候变化认识的重要补充。