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21.
The study of interactions between minerals, organic matter (OM) and microorganisms is essential for the understanding of soil functions such as OM turnover. Here, we present an interdisciplinary approach using artificial soils to study the establishment of the microbial community and the formation of macro-aggregates as a function of the mineral composition by using artificial soils. The defined composition of a model system enables to directly relate the development of microbial communities and soil structure to the presence of specific constituents. Five different artificial soil compositions were produced with two types of clay minerals (illite, montmorillonite), metal oxides (ferrihydrite, boehmite) and charcoal incubated with sterile manure and a microbial community derived from a natural soil. We used the artificial soils to analyse the response of these model soil systems to additional sterile manure supply (after 562 days). The artificial soils were subjected to a prolonged incubation period of more than two years (842 days) in order to take temporally dynamic processes into account. In our model systems with varying mineralogy, we expected a changing microbial community composition and an effect on macro-aggregation after OM addition, as the input of fresh substrate will re-activate the artificial soils. The abundance and structure of 16S rRNA gene and internal transcribed spacer (ITS) fragments amplified from total community DNA were studied by quantitative real-time PCR (qPCR) and denaturing gradient gel electrophoresis (DGGE), respectively. The formation of macro-aggregates (>2 mm), the total organic carbon (OC) and nitrogen (N) contents, the OC and N contents in particle size fractions and the CO2 respiration were determined. The second manure input resulted in higher CO2 respiration rates, 16S rRNA gene and ITS copy numbers, indicating a stronger response of the microbial community in the matured soil-like system. The type of clay minerals was identified as the most important factor determining the composition of the bacterial communities established. The additional OM and longer incubation time led to a re-formation of macro-aggregates which was significantly higher when montmorillonite was present. Thus, the type of clay mineral was decisive for both microbial community composition as well as macro-aggregation, whereas the addition of other components had a minor effect. Even though different bacterial communities were established depending on the artificial soil composition, the amount and quality of the OM did not show significant differences supporting the concept of functional redundancy. 相似文献
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《Communications in Soil Science and Plant Analysis》2012,43(16):2488-2495
This article highlights the results of a long-term research project on the production of grape stalks engrafted by the desk method. The integrated impact of rootstock varieties, modes of stratification, and different substrates on vegetating vine cuttings, raised in heated greenhouses, and the capacity of their acclimatization after planting was studied. The results of the research show that rootstock variety and modes of stratification influence the output of engrafted vegetating cuttings of vines grown in pots using various substrates. In particular, the engrafted cuttings of variety Rkatsiteli produced on two rootstocks, 5 BB and 101-14, passed stratification (a) in sawdust with local electroheating, (b) on the water with its periodic change, and (c) in the layer of perlite. After preplanting preparations they were planted in pots with six different substrates: (1) soil (control); (2) perlite; (3) sawdust; (4) rice husk + soil + sand (1:1:1); (5) peat + soil + sand (1:1:1); and (6) mold + soil + sand (1:1:1). The cuttings were grown with a covered root system in a heated greenhouse for 35–40 days. Forty-day-old vegetating cuttings, after hardening, were planted into the open ground. Specialties were established during the root and shoot formation on vegetating nursery plant grafts during the rooting period. Optimal substrates for growing engrafted cuttings with a covered root system in heated greenhouses for each rootstock and stratification mode were determined. 相似文献
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应用压力早期恢复资料确定低渗地层压力 总被引:3,自引:0,他引:3
低渗透油藏的压力恢复曲线,不能用Horner外推压力求取地层压力。基于Horner方程的等轴双曲线形式,提出了应用压力早期恢复资料确定低渗地层压力的新方法。通过油井实际资料计算验证,用该方法计算的地层压力准确可靠,较好地解决了低渗透油藏求地层压力的问题。 相似文献
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于2017—2018年和2018—2019年,在西北中部旱作雨养农业区以冬小麦‘康庄974’为试验材料,设秸秆带状覆盖(SM)、地膜覆盖(PM)和无覆盖对照(CK)共3个栽培处理,分析不同覆盖方式对小麦灌浆期土壤水分和温度及抗氧化能力的影响,探讨花后干物质积累量和粒重形成的关系。结果表明,随生育期推进,花后旗叶相对含水量(RWC)逐渐降低,丙二醛(MDA)含量逐渐升高,且PM较SM降、升幅度明显。与CK相比,覆盖显著提高花后旗叶抗氧化酶活性,增加渗透调节物质含量;开花时间越长,SM抗氧化酶活性升高的幅度越大,而PM主要提高花后7 d旗叶抗氧化能力。SM和PM的粒重分别较CK增加14.3%和19.1%(P<0.05)。相关分析表明,土壤水分(SW)是影响旗叶生理活性的关键因子,提高SW,有利于增加RWC(r=0.84**),从而提高旗叶抗氧化能力,其中RWC和抗坏血酸酶(ASA)活性呈极显著正相关关系(r=0.82**);ASA活性和脯氨酸(Pro)含量呈极显著正相关关系(r=0.94**),和MDA含量呈极显著负相... 相似文献
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产业融合共生是新时期红色旅游的必然选择,红色旅游企业共生系统可分为区域内共生子系统和区域外共生子系统,表现为核心共生、主要共生和跨界共生三种形态,核心共生形态是红色旅游企业共生区别其他企业共生的根本所在。红色旅游企业共生由合作和共识耦合而成,但核心共生形态的形成机理更多地表现为天然性,要求红色旅游企业主动寻求合作,争取与其他共生单元达成共识,促进红色旅游企业共生关系的良性发展。 相似文献