井渠结合灌溉类型区多水源联网优化调度模式研究

针对丘陵地区灌溉水源分散、灌溉保证率低、农业缺水严重、生态环境恶化等突出问题,将多水源联网工程技术与优化调度技术相结合,建立了井渠结合灌区多水源联网优化调度模型,大大提高了灌区水资源的利用效率和灌溉保证率。采用该技术及其他节水措施后,威海井渠结合节水示范区全部耕地可得到灌溉,灌溉后的粮食产量由原来的9000 kg/hm2提高到11550 kg/hm2,示范区平均增产25%。经济、社会与环境效益显著。     

小型猪特异性麻醉颉颃剂对大鼠脑NO/cGMP信号转导系统的动态影响

为探讨NO/cGMP信号转导系统对小型猪特异性麻醉颉颃剂催醒分子机理的调控,144只Wistar大鼠随机分为对照组和试验组,对照组和试验组再分为早期催醒组、中期催醒组和晚期催醒组。用分光光度法测定脑NOS活性和NO产量,ELISA测定脑cGMP含量。结果显示,小型猪特异性麻醉颉颃剂能明显地激活大鼠相关脑区的NOS活性和NO及cGMP产量,并且大鼠不同脑区NOS活性、NO产量和cGMP含量的变化趋势不仅与大鼠行为学变化相平行,还与不同时期表现出的催醒效果基本吻合。这表明小型猪特异性麻醉颉颃剂的催醒可能与激活特定脑区的NO/cGMP信号转导系统相关。     

牛羊4种寄生虫病联合诊断技术的研究与应用

疫区牛羊同时寄生有捻转血矛线虫、日本血吸虫、伊氏锥虫和肝片吸虫等多种寄生虫。目前血清学诊断方法对这 4种寄生虫病的检测需 2～ 4次操作。将捻转血矛线虫、日本血吸虫、伊氏锥虫和肝片吸虫抗原分别以 0 1 5 ,0 0 4 ,0 0 6和 0 1 3μg/ μL的最佳浓度依次定点在同一硝酸纤维素膜条上 ,制成 4种寄生虫病联合诊断膜。用黄牛IgG ,水牛IgG和山羊IgG联合免疫兔 ,获得了高效价兔抗黄牛、水牛和山羊 (简称兔抗牛羊 )IgG抗血清。应用兔抗牛羊IgG抗血清连接酶标SPA ,建立了 1份血纸能同时用于检测牛、羊捻转血矛线虫、日本血吸虫、伊氏锥虫和肝片吸虫病抗体技术。经浙江、湖北、四川、安徽等省应用 ,该项技术具有省工、省时、经济、实用等优点。适宜于牛、羊捻转血矛线虫、日本血吸虫、伊氏锥虫和肝片吸虫病流行区的普查或监测     

Genetic relationships between the dominant genotypes of Phytophthora infestans in Hokkaido, Japan

The genetic characteristics of the dominant genotypes of Phytophthora infestans in Japan (US-1, JP-1, Japanese A1-A, A1-B) were compared. Differences were evident in the peptidase genotype, amplified fragment length polymorphism, and RG57 DNA fingerprints. Almost all of the fingerprint bands for the Japanese genotype A1-B were also present in JP-1 and Japanese A1-A, and few bands were unique to Japanese A1-B. These results suggest that the Japanese A1-B genotype was generated from sexual reproduction involving Japanese A1-A and JP-1 or related genotypes.     

The loss of genetic diversity in Dabry's sturgeon (Acipenser dabryanus,Dumeril) as revealed by DNA fingerprinting

• 1. Dabry's sturgeon, a large, long‐lived migratory fish is endemic to the Yangtze River. Over‐fishing and habitat destruction have caused large‐scale declines in natural stocks in the last two decades.
• 2. Examining patterns of genetic diversity has become an integral component of many management plans for endangered species. DNA fingerprinting was applied to detect genetic diversity in Dabry's sturgeon collected in 1958–1959, 1980–1981 and 1998–1999.
• 3. Studies on direct genetic parameters (genetic variability, hypervariable loci and heterozygosity) and indirect parameters (band‐sharing coefficient and allelic frequency) showed that the continuous decline in wild populations has caused the loss of genetic diversity in present‐day sturgeon.
• 4. The present‐day populations have the lowest genetic variability; thus, effective management is needed to preserve genetic diversity.
• 5. A conservation strategy is urgently required, comprising artificial rearing facilities coupled with breeding management plans.

Copyright © 2003 John Wiley & Sons, Ltd.     

DNA fingerprinting of Australian isolates of Mycobacterium avium subsp paratuberculosis using IS900 RFLP

OBJECTIVES: To evaluate additional restriction enzymes for IS900 RFLP of Mycobacterium avium subsp paratuberculosis and examine the genetic diversity among Australian isolates for epidemiological studies of Johne's disease. DESIGN AND PROCEDURE: Seventy-one isolates of M paratuberculosis from cattle, sheep, goat, alpaca and rhinoceros in six Australian States and the Northern Territory, reference strains and reference DNA from previously characterised strains were tested for genetic variation. Bst EII, Pvu II and Pst I restriction enzymes were used, and four others (Bam HI, Alu I, Xho I and Dra I) were assessed for their ability to detect polymorphisms. Multiple isolates from some animals were tested. RESULTS: Bam HI, was the most effective enzyme for identifying polymorphisms (12 types), followed by Bst EII (11 types). Both Pvu II and Pst I were relatively ineffectual. Fifteen different types were identified, 12 in clinical isolates. Most isolates were cattle (C) strains and fell into the C1 (n = 28) and C3 (n = 32) groupings. All isolates from alpaca were type C1, and bovine isolates were commonly C1 (n = 15) or C3 (n = 28). All of the sheep were infected with sheep (S) strains; no S strains were identified in cattle. Two of six isolates from one animal had single band differences. CONCLUSION: The epidemiological features of M paratuberculosis in Australia are similar to those reported in New Zealand, where cattle and sheep are commonly infected with different strains. However, because of the lack of polymorphism identified within the major groups, it is unlikely that DNA fingerprinting will have a significant role in epidemiological studies of Johne's disease, unless an unusual strain in being studied.     

甲霜灵·刺复配剂对棉疫病菌的联合毒力

在实验室条件下，测定了甲霜灵和霜霉威复配剂对棉疫病菌（Phytophthora boehmeriae）的联合毒力。结果表明，复配剂MPA、MPB、MPC对棉疫病菌的DC5。分别为0．0555,0．1044μg／mL和0．0845μg／mL，对照单剂甲霜灵和霜霉威对棉疫病菌的EC50分别为0．0391μg／mL和10．6183μg／mL；联合毒力分析表明，甲霜灵和霜霉威按1：1．5复配有增效作用，按1：1和1．5：1复配有相减作用。     

稻油轮作区驱动圆盘犁对置组合式耕整机设计与试验

针对长江中下游稻油轮作区油菜种植时土壤黏重板结、秸秆量大、播种作业需同步开畦沟的农艺要求,考虑传统耕整作业耕层浅、功耗大的不足,依据驱动圆盘犁组与传统铧式犁相比,不易缠草堵塞、通过性好、牵引阻力小的特征,设计了用于油菜播种的驱动圆盘犁对置组合式耕整机。提出了主动式对置犁耕与被动式开畦沟、碎土、平整相结合的联合耕整作业方案,分析了对称布置的圆盘犁组的动力学和运动学特性,确定了其主要结构和工作参数。根据犁体曲面成形原理,设计了开畦沟前犁犁体曲面;依据组合式船型开沟器与土壤挤压互作机制的分析,确定了开畦沟区域宽度为350 mm时,开畦沟系统作业后可有效保证畦沟和种床厢面质量。耕深稳定性试验表明,整机作业实际耕深与限深深度基本一致,耕深稳定性系数均在90%以上。厢面质量试验表明,开畦沟系统在中间开畦沟区域能开出沟宽241.6~293.5 mm,沟深328.6~370.8 mm的梯形沟。经组合式船型开沟器挤压的土壤对犁沟的实际填埋率高于87.67%,碎土辊作业后厢面平整度为22.45~26.70 mm,碎土率为60.14%~68.37%。正交试验结果表明,整机较优工作参数为:限深深度为180 mm,机组前进速度为3.5 km/h,圆盘犁组转速为160 r/min,此时整机功耗为24.37 k W,相比传统旋耕方式的油菜播种种床整备机具的功耗降低了37.67%,秸秆埋覆率为92.78%,碎土率为66.74%,厢面平整度为24.18 mm,土壤对犁沟平均填埋率为92.3%,满足油菜播种的农艺要求。     

采伐联合机液压驱动控制系统研究

介绍了采伐联合机的基本结构和工作原理,建立了液压驱动控制系统数学模型,为进一步研究机械手的驱动控制系统奠定了基础.     

Tracing Sediment Sources Using Mid‐infrared Spectroscopy in Arvorezinha Catchment,Southern Brazil

The widespread adoption of the sediment fingerprinting approach to guide catchment management has been limited by the cost and the difficulty to prepare and process samples for geochemical and radionuclide analyses. Spectral properties have recently been shown to provide a rapid and cost‐efficient alternative for this purpose. The current research objective was (i) to quantify the sediment source contributions in a 1∙19‐km² rural catchment of Southern Brazil by using mid‐infrared (MIR) spectroscopy and (ii) to compare these results with those obtained with geochemical approach and near‐infrared and ultraviolet–visible spectroscopy methods. The sediment sources to discriminate were cropland surface (n  = 20), unpaved roads (n  = 10) and stream channel banks (n  = 10). Twenty‐nine suspended sediment samples were collected at the catchment outlet during nine significant flood events. The sources could be distinguished by MIR spectroscopy. Cropland and channel bank sources mainly differed in their clay mineral contents, but their similar organic matter content complicated the MIR‐model predictions. Unpaved road contributions were discriminated from the other sources by their lower organic carbon content. When the results of the current research based on MIR spectroscopy are compared with those obtained using other sediment fingerprinting approaches, based on geochemistry and near‐infrared and ultraviolet–visible spectroscopy, an overestimation of channel banks contribution and an underestimation of cropland and unpaved road contributions is found. These results suggest that MIR spectroscopy can provide a useful tool that is non‐destructive, rapid and cheap for tracing sediment sources in rural catchments and for guiding the implementation of soil and water conservation measures. Copyright © 2016 John Wiley & Sons, Ltd.