国槐种子超声波处理效应研究

为了探索超声波处理对国槐种子萌发的影响,利用47、59 KHz超声波对国槐种子进行了超声波处理.结果表明:用59 KHz超声波处理种子10 min效果最好,可以使国槐种子萌发率提高及发芽指数分别提高44.3％;59 KHz超声波处理种子5min后可溶性糖含量比对照值提高32.5％,淀粉含量提高7.96％;处理时间大于25 min,会明显降低种子淀粉含量;超声波处理对种子电导率及可溶性蛋白质含量没有明显的影响.     

反枝苋种子休眠解除方法研究

研究了光照和黑暗两种条件下氢氧化钠（NaoH）、盐酸（HC l）、赤霉素（GA3）和乙烯利（ETH）处理对反枝苋种子萌发的影响。结果表明,氢氧化钠、盐酸、赤霉素和乙烯利浸泡处理均可以明显提高反枝苋种子的发芽率,且随着试剂浓度的提高,发芽率表现出单峰变化曲线,浓度过低或过高均不利于种子的萌发。其中,赤霉素对解除反枝苋种子休眠最有效,且以200 mg/L浸泡处理24 h效果最好,发芽率可达90.8%,比对照提高了52.5百分点。其次为4%NaOH和6%HC l处理1 h,与800 mg/L ETH处理24 h的效果相当,发芽率在70%~75%之间。黑暗条件不利于反枝苋种子的萌发,发芽率略低于光照条件。     

温度对4种杂草种子萌发的影响

研究了20℃/15℃和25℃/20℃两种日波动温度对棒头草（Polypogon fugax Nees ex steud.）、大瓜草（Spergula arvensis L.）、红花月见草（Oenothera rosea L’Herit.ex Ait.）和紫茎泽兰（Eupatorium adenophorumSpreng.）种子的发芽率、发芽势和发芽指数的作用。结果表明,4种杂草种子萌发对两种波动温度的反应存在差异。前4 d高温有利于棒头草种子萌发,而3 d以后,种子在低温时的发芽率均高于高温处理。前3 d高温促进大瓜草种子的发芽势,而4 d后两种温度下种子的发芽率维持不变,且低温处理的发芽率比高温处理高10%左右。红花月见草和紫茎泽兰种子萌发对温度的反应很相似,即在种子萌发的早期高温和低温种子发芽率差异很大,随着发芽时间的进一步延长,两种温度处理的发芽率逐渐接近。     

茶树“二段法”快繁育苗适宜水质及营养液pH值研究初报

《茶树无性系良种"二段法"快繁育苗技术研究》为湖北省恩施农科院茶叶研究所自2005年以来的攻关课题,旨在解决山区茶农茶苗移栽成活率低的瓶颈问题,加快武陵山区无性系良种化建设进程。该技术成功实现了工厂化育苗、半年育苗出圃、带土坨移栽等重要技术指标,可提高茶苗移栽成活率95%以上,茶苗移栽无缓苗期,因营养液水质及pH值对短穗水培愈合及生根起到比较关键的作用。本文就营养液选择适宜pH值的试验作简要介绍,以供广大育苗单位及爱好者参考,较常规育苗技术提前1年获高产,项目成果推广性很强。     

黏籽西瓜与籽用西瓜种皮性状的遗传研究

通过利用黏籽西瓜独特的黏籽性状与籽瓜亚种间进行杂交,对所配6个世代中的黏籽种皮类型与籽瓜种皮类型的分离比例进行分析和采用卡方检验,来验证理论值与观测值间的关系。结果表明,在F1代中黏籽性状不表现,而在与黏籽西瓜作父本的BC1代中籽瓜种皮类型和黏籽种皮类型分离比例为1:1,在F2代中籽瓜种皮类型和黏籽种皮类型分离比例为3:1。由此可以推测黏籽性状是由隐性单基因控制的。     

腾格里沙漠南缘3种优势植物种子出苗对土壤类型和沙埋的响应

沙米（Agriophyllum squarrosum）、沙蒿（Artemisia sphaerocephala）和油蒿（Artemisia ordosica）分别是腾格里沙漠南缘不同植被演替阶段的3个优势物种,2009年5～6月在大田盆栽条件下,设计了2种土壤类型和0 cm,1 cm,2 cm,3 cm,4 cm,6 ...     

Effectiveness of thiamethoxam and imidacloprid seed treatments against Bemisia tabaci (Hemiptera: Aleyrodidae) on cotton

BACKGROUND: Bemisia tabaci (Gennadius) biotype B is one of the most important pests on cotton around the world. Laboratory, greenhouse and field experiments were conducted to determine the efficacy of thiamethoxam and imidacloprid seed treatments against B. tabaci on cotton. RESULTS: Under laboratory conditions, the two treatments caused whitefly adult mortality, reduced oviposition and increased mortality of nymphs at 10, 20, 30 and 40 days after germination (DAG). The longer the adults fed on plants from treated seeds, the higher the mortality. The two treatments did not have any effect on eggs. The efficacy of the treated seeds against B. tabaci gradually decreased from 10 to 40 DAG, being the lowest at 40 DAG. In laboratory experiments, the efficacies between the two treatments were similar. In greenhouse experiments, the two treatments were equally effective with lower numbers of whiteflies than untreated controls. With both treatments the concentrations of the active ingredient were gradually reduced with aging of the plants and from the bottom to the top leaves of the plants. Numbers of live whiteflies were well correlated with the dosage of active ingredients. Under field conditions, the seeds treated with both insecticides exhibited similar efficacy against B. tabaci for up to ～2 months. CONCLUSION: Cotton seeds treated with imidacloprid and thiamethoxam were effective against B. tabaci for up to 45 days under laboratory and greenhouse conditions, and up to ～2 months under field conditions. Use of imidacloprid‐ and thiamethoxam‐treated seeds can be an important alternative for management of whiteflies on cotton. Copyright © 2010 Society of Chemical Industry     

Analysis of the seed dispersal patterns of wild soybean as a reference for vegetation management around genetically modified soybean fields

There are concerns that genetically modified soybean might threaten the genetic diversity of the wild soybean populations that are distributed in East Asia because genetically modified soybean has no crossing barrier with wild soybean. A simple and effective method to prevent hybridization via pollen flow is spatial separation between the two species because their hybridization occurs only when they grow in close proximity. Therefore, the invasiveness of wild soybean needs to be known in order to secure the appropriate distances. As wild soybean seeds are dispersed mechanically by pod dehiscence, an experiment was conducted in which white sheets were placed on the ground, concentric circles were drawn around the parent plants, and the number of dispersed seeds within each 0.5 m‐wide zone were counted. About 40% of the produced seeds were dispersed and the number of dispersed seeds gradually declined as the distance from the parent plants increased. The model that explained the relationship between the number and distance of the dispersed seeds was produced by using a generalized linear model procedure. More than 95, 99, and 99.9% of the produced seeds stayed within 3.5, 5.0, and 6.5 m after natural pod dehiscence. Knowing these values is useful for evaluating the level of invasive risk by mechanical seed dispersal. The goal of the work is to efficiently and deliberately prevent hybridization by isolating genetically modified soybean fields and wild soybean populations by vegetation management, including weeding and setting up specific‐width buffer zones.     

Integration of soil application and seed treatment formulations of Trichoderma species for management of wet root rot of mungbean caused by Rhizoctonia solani

BACKGROUND: The efficacy of seed dressing and soil application formulations from the isolates of Trichoderma viride (IARI P1; MTCC 5369), T. virens (IARI P3; MTCC 5370) and T. harzianum (IARI P4; MTCC 5371) were evaluated individually and in combination in pot and field experiments during the rainy seasons of 2005, 2006 and 2007 for the management of wet root rot (Rhizoctonia solani) and improvement in the yield of mungbean. RESULTS: A seed dressing formulation, Pusa 5SD, and soil application formulations, Pusa Biogranule 6 (PBG 6) and Pusa Biopellet 16G (PBP 16G), based on Trichoderma virens, were found to be superior to other formulations in reducing disease incidence and increasing seed germination and shoot and root lengths in mungbean. In field experiments, a combination of soil application with PBP 16G (T. virens) and seed treatment with Pusa 5SD (T. virens) + carboxin was superior to any of these formulations individually in increasing seed germination, shoot and root lengths and grain yield and reducing wet root rot incidence in mungbean. Seed treatment was more effective than soil application for all the evaluated parameters. The combined application of Pusa 5SD and carboxin was also superior to individual treatment. CONCLUSION: The efficacy of the evaluated formulations against wet root rot of mungbean proved that the integration of soil application of PBP 16G and seed treatment with Pusa 5SD + carboxin is highly effective for the management of wet root rot, increasing plant growth and grain yield of mungbean Copyright © 2011 Society of Chemical Industry