Copper‐Induced Oxidative Stress and Responses of the Antioxidant System in Roots of Medicago sativa

The effects of various copper (Cu) concentrations on the antioxidative system in the roots of Medicago sativa were explored. The results indicated that the Cu content of the roots reached a value of 854 μg g^?1 DW at 10 μm Cu and a value of 4415 μg g^?1 DW at 100 μm Cu, suggesting that M. sativa has better ability to tolerate and accumulate Cu than other Cu‐bioaccumulators, and is a potential plant for phytoremediation. Treatment with Cu resulted in a significant increment in the levels of H₂O₂, O₂˙^? and OH˙. The reduced form of ascorbate and glutathione reached a peak at 30 μm Cu, and was followed by a sharp depletion to a lower level than that of the control. In contrast, the levels of the oxidised forms of ascorbate and glutathione showed a progressive increment with increasing Cu concentrations, suggesting that the antioxidant system was unable to cope with Cu stress at higher Cu levels. Under the Cu concentrations tested, the activity of catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) increased at lower Cu concentrations, and then decreased, reaching a maximum at 30 μm of Cu for APX and GR, at 10 μm for CAT, whereas the activities of guaiacol peroxidase (POD, EC 1.11.1.7) were gradually increased with increasing Cu concentrations. PAGE analysis of superoxide dismutase (SOD, EC 1.1.5.1.1) revealed that one band is a Mn‐SOD and five bands are identified as Cu, Zn‐SOD, whereas Fe‐SOD isoforms were not found in the roots of alfalfa. Cu at 10–100 μm increased the intensity of constitutive isozymes of CAT, APX and POD, whereas it decreased the intensity of isozymes of glucose‐6‐phosphate dehydrogenase (G6PDH, EC 1.1.1.49) significantly. The activities of lipoxygenases (LOX, EC 1.13.11.12) were gradually augmented with increasing Cu concentrations, demonstrating that LOXs are probably involved in production of lipid hydroperoxides and superoxide anion. There was a continuous and pronounced enhancement in the activity of esterase (EST, EC 3.1.1.1) in roots treated with 10–30 Cu μm , whereas EST activity in roots exposed to above 30 μm Cu declined, suggesting that EST plays a protective role under lower Cu concentrations stress.     

Organic farming fosters agroecosystem functioning in Argentinian temperate soils: Evidence from litter decomposition and soil fauna

Benefits of organic farming on soil fauna have been widely observed and this has led to consider organic farming as a potential approach to reduce the environmental impact of conventional agriculture. However, there is still little evidence from field conditions about direct benefits of organic agriculture on soil ecosystem functioning. Hence, the aims of this study were to compare the effect of organic farming versus conventional farming on litter decomposition and to study how this process is affected by soil meso- and macrofauna abundances. Systems studied were: (1) organic farming with conventional tillage (ORG), (2) conventional farming with conventional tillage (CT), (3) conventional farming under no-tillage (NT), and (4) natural grassland as control system (GR). Decomposition was determined under field conditions by measuring weight loss in litterbags. Soil meso- and macrofauna contribution on decomposition was evaluated both by different mesh sizes and by assessing their abundances in the soil. Litter decomposition was always significantly higher after 9 and 12 months in ORG than in CT and NT (from 2 to 5 times in average), regardless decomposer community composition and litter type. Besides, mesofauna, macrofauna and earthworm abundances were significantly higher in ORG than in NT and CT (from 1.6 to 3.8, 1.7 to 2.3 and 16 to 25 times in average, respectively for each group). These results are especially relevant firstly because the positive effect of ORG in a key soil process has been proved under field conditions, being the first direct evidence that organic farming enhances the decomposition process. And secondly because the extensive organic system analyzed here did not include several practices which have been recognized as particularly positive for soil biota (e.g. manure use, low tillage intensity and high crop diversity). So, this research suggests that even when those practices are not applied, the non-use of agrochemicals is enough to produce positive changes in soil fauna and so in decomposition dynamics. Therefore, the adoption of organic system in an extensive way can also be suggested to farmers in order to improve ecosystem functioning and consequently to achieve better soil conditions for crop production.     

Impact of soil engineering by two contrasting species of earthworms on their dispersal rates

By burrowing galleries and producing casts, earthworms are constantly changing the structure and properties of the soils in which they are living. These changes modify the costs and benefits for earthworms to stay in the environment they modify. In this paper, we measured experimentally how dispersal behaviour of endogeic and anecic earthworms responds to the cumulative changes they made in soil characteristics. The influence of earthworm activities on dispersal was studied in standardised mesocosms by comparing the influence of soils modified or not modified by earthworm activities on earthworm dispersal rates.The cumulative use of the soil by the earthworms strongly modified soil physical properties. The height of the soil decreased over time and the amount of aggregates smaller than 2 mm decreased in contrast to aggregates larger than 5 mm that increased. We found that: (i) earthworm activities significantly modified soil physical properties (such as bulk density, soil strength and soil aggregation) and decreased significantly the dispersal rates of the endogeic species, whatever the species that modified the soil; (ii) the decreasing in the dispersal proportion of the endogeic species suggests that the cost of engineering activities may be higher than the one of dispersal; (iii) the dispersal of the anecic species appeared to be not influenced by its own activities (intra-specific influences) or by the activities of the endogeic species (inter-specific influences). Overall these results suggest that the endogeic species is involved in a process of niche construction, which evolved jointly with its dispersal strategy.     

河北省土壤有机质时空变化分析

采用BP神经网络和地统计方法,对1980~2009年间河北省耕地土壤有机质时空变化规律及影响因素进行对比分析。结果表明:河北省耕地土壤有机质具有西北高东南低的格局;随着时间的推移,有机质总体呈现上升趋势。1980年的低值区域到了2009年SOM上升变化明显,成为热点;北部坝上、西部太行山区及东部沿海地区到2009年SOM含量下降,成为冷点。分析表明,这种变化格局的形成与施用有机肥、实施秸秆还田等社会经济过程有关。本研究为预测有机质变化趋势、改善土壤质量、优化种植结构和耕地的可持续发展提供了一定依据。     

The Relationship Between Vegetation Characteristics and Altitudes in Transitional Permafrost Zone in Xidatan,Qinghai-Tibetan Plateau

Many studies showed that permafrost has profound influence on alpine ecosystem. However, former researches were mainly focused on typical points by temporal scales. There were few studies about the correlation between vegetation characteristics and different altitudes covering a large region in spatial pattern, especially in transitional permafrost(TP). There were continuous permafrost(CP) discontinuous permafrost(DCP) and seasonal frozen ground(SFG) in this study region. The types of permafrost changed from SFG to DCP, and finally become CP as the altitudes of Xidatan increase. In this paper, 112 845 points interpreted by HJ1-B(environment and disaster monitoring and prediction small satellite constellation), vegetation investigation points, thawing layer thickness research sites, ground temperature and water content observation plots were used to examine the spatial pattern of vegetation which were located in different altitudes in Xidatan, a typical TP region, in Qinghai-Tibetan Plateau. Vegetation characteristics, soil moisture content(SMC) and thaw depths were collected in 15 August to 25 August2012. Characteristics of vegetation were mainly represented by fractional vegetation cover(FVC) derived from the normalized difference vegetation index(NDVI), as well as above ground biomass(AGB). In this paper, we analyzed that the distinction of vegetation characteristics in each range through statistics data. These ranges were divided by varied altitudes. For examples, the ranges were divided into 50 m or 100 m. In this study we use a large area plots method to further discuss the relationship between the features of vegetation and the different regions of permafrost based on altitudes shifts in Xidatan. A diagram described the vegetation characteristics variability with rising altitudes in transitional permafrost region was drawn in this paper. Our results illustrated the FVCs first increased in SFG region and then decreased in DCP zone slowly, and in CP region FVCs soared then dropped dramatically. With the altitudes increased, the curve of FVCs indicated a parabolic distribution except a little difference in the first 200 m range.     

Short time effects of biological and inter-row subsoiling on yield of potatoes grown on a loamy sand,and on soil penetration resistance,root growth and nitrogen uptake

Soil compaction, especially subsoil compaction, in agricultural fields has increased due to widespread use of heavy machines and intensification of vehicular traffic. Subsoil compaction changes the relative distribution of roots between soil layers and may restrict root development to the upper part of the soil profile, limiting water and mineral availability. This study investigated the direct effects of inter-row subsoiling, biological subsoiling and a combination of these two methods on soil penetration resistance, root length density, nitrogen uptake and yield. In field experiments with potatoes in 2013 and 2014, inter-row subsoiling (subsoiler) and biological subsoiling (preceding crops) were studied as two potential methods to reduce soil penetration resistance. Inter-row subsoiling was carried out post planting and the preceding crops were established one year, or in one case two years, prior to planting. Soil resistance was determined with a penetrometer three weeks after the potatoes were planted and root length density was measured after soil core sampling 2 months after emergence. Nitrogen uptake was determined in haulm (at haulm killing) and tubers (at harvest). Inter-row subsoiling had the greatest effect on soil penetration resistance, whereas biological subsoiling showed no effects. Root length density (RDL) in the combined treatment was higher than in the separate inter-row and biological subsoiling treatments and the control, whereas for the separate inter-row and biological subsoiling treatments, RLD was higher than in the control. Nitrogen uptake increased with inter-row subsoiling and was significantly higher than in the biological subsoiling and control treatments. However, in these experiments with a good supply of nutrients and water, no yield differences between any treatments were observed.     

Evaluating biodegradability of soil organic matter by its thermal stability and chemical composition

The stability of soil organic matter (SOM) as it relates to resistance to microbial degradation has important implications for nutrient cycling, emission of greenhouse gases, and C sequestration. Hence, there is interest in developing new ways to quantify and characterise the labile and stable forms of SOM. Our objective in this study was to evaluate SOM under widely contrasting management regimes to determine whether the variation in chemical composition and resistance to pyrolysis observed for various constituent C fractions could be related to their resistance to decomposition. Samples from the same soil under permanent pasture, an arable cropping rotation, and chemical fallow were physically fractionated (sand: 2000-50 μm; silt: 50-5 μm, and clay: <5 μm). Biodegradability of the SOM in size fractions and whole soils was assessed in a laboratory mineralization study. Thermal stability was determined by analytical pyrolysis using a Rock-Eval pyrolyser, and chemical composition was characterized by X-ray absorption near-edge structure (XANES) spectroscopy at the C and N K-edges. Relative to the pasture soil, SOM in the arable and fallow soils declined by 30% and 40%, respectively. The mineralization bioassay showed that SOM in whole soil and soil fractions under fallow was less susceptible to biodegradation than that in other management practices. The SOM in the sand fraction was significantly more biodegradable than that in the silt or clay fractions. Analysis by XANES showed a proportional increase in carboxylates and a reduction in amides (protein) and aromatics in the fallow whole soil compared to the pasture and arable soils. Moreover, protein depletion was greatest in the sand fraction of the fallow soil. Sand fractions in fallow and arable soils were, however, relatively enriched in plant-derived phenols, aromatics, and carboxylates compared to the sand fraction of pasture soils. Analytical pyrolysis showed distinct differences in the thermal stability of SOM among the whole soil and their size fractions; it also showed that the loss of SOM generally involved preferential degradation of H-rich compounds. The temperature at which half of the C was pyrolyzed was strongly correlated with mineralizable C, providing good evidence for a link between the biological and thermal stability of SOM.     

Environmental stress response limits microbial necromass contributions to soil organic carbon

The majority of dead organic material enters the soil carbon pool following initial incorporation into microbial biomass. The decomposition of microbial necromass carbon (C) is, therefore, an important process governing the balance between terrestrial and atmospheric C pools. We tested how abiotic stress (drought), biotic interactions (invertebrate grazing) and physical disturbance influence the biochemistry (C:N ratio and calcium oxalate production) of living fungal cells, and the subsequent stabilization of fungal-derived C after senescence. We traced the fate of ¹³C-labeled necromass from ‘stressed’ and ‘unstressed’ fungi into living soil microbes, dissolved organic carbon (DOC), total soil carbon and respired CO₂. All stressors stimulated the production of calcium oxalate crystals and enhanced the C:N ratios of living fungal mycelia, leading to the formation of ‘recalcitrant’ necromass. Although we were unable to detect consistent effects of stress on the mineralization rates of fungal necromass, a greater proportion of the non-stressed (labile) fungal necromass C was stabilised in soil. Our finding is consistent with the emerging understanding that recalcitrant material is entirely decomposed within soil, but incorporated less efficiently into living microbial biomass and, ultimately, into stable SOC.     

Contrasting development of soil microbial community structure under no-tilled perennial and tilled cropping during early pedogenesis of a Mollisol

A range of agricultural practices influence soil microbial communities, such as tillage and organic C inputs, however such effects are largely unknown at the initial stage of soil formation. Using an eight-year field experiment established on exposed parent material (PM) of a Mollisol, our objectives were to: (1) to determine the effects of field management and soil depth on soil microbial community structure; (2) to elucidate shifts in microbial community structure in relation to PM, compared to an arable Mollisol (MO) without organic amendment; and (3) to identify the controlling factors of such changes in microbial community structure. The treatments included two no-tilled soils supporting perennial crops, and four tilled soils under the same cropping system, with or without chemical fertilization and crop residue amendment. Principal component (PC) analysis of phospholipid fatty acid (PLFA) profiles demonstrated that microbial community structures were affected by tillage and/or organic and inorganic inputs via PC1 and by land use and/or soil depth via PC2. All the field treatments were separated by PM into two groups via PC1, the tilled and the no-tilled soils, with the tilled soils more developed towards MO. The tilled soils were separated with respect to MO via PC1 associated with the differences in mineral fertilization and the quality of organic amendments, with the soils without organic amendment being more similar to MO. The separations via PC1 were principally driven by bacteria and associated with soil pH and soil C, N and P. The separations via PC2 were driven by fungi, actinomycetes and Gram (−) bacteria, and associated with soil bulk density. The separations via both PC1 and PC2 were associated with soil aggregate stability and exchangeable K, indicating the effects of weathering and soil aggregation. The results suggest that in spite of the importance of mineral fertilization and organic amendments, tillage and land-use type play a significant role in determining the nature of the development of associated soil microbial community structures at the initial stages of soil formation.     

Effects of spent mushroom substrates and inorganic fertilizer on the characteristics of a calcareous clayey‐loam soil and lettuce production

We evaluated the effects of the addition of two types of spent mushroom substrate (SMS), SMS from an Agaricus bisporus crop (SMS1) and a mixture of SMSs from an A. bisporus crop and a Pleurotus crop (50% v/v each) (SMS2), on the characteristics of a calcareous clayey‐loam soil and the yield and nutritional status of lettuce (Lactuca sativa L.), relative to crops receiving mineral fertilizer (M) and a control (C) without amendment. The application of SMS, especially SMS1, improved soil fertility compared with C and M soils. Moreover, the use of these organic substrates as soil amendments did not harm the plants and gave yields similar to that obtained with mineral fertilizer. The nutritional contents of the lettuce plants were greater than or similar to those of the plants from treatment C or M, except for the plant tissue concentrations of K, Fe and Zn, which were significantly reduced by SMS application. However, this latter fact did not reduce the lettuce yield in the amended soils. Therefore, the use of SMSs as organic amendments contributes to residue utilization, in an environmentally friendly way, and to improved soil fertility and crop yield.