Bacterial community structure and microbial activity in different soils amended with biogas residues and cattle slurry

Anaerobic digestion of organic materials generates residues of differing chemical composition compared to undigested animal manures, which may affect the soil microbial ecosystem differently when used as fertilizers. This study investigated the effects of two biogas residues (BR-A and BR-B) and cattle slurry (CS) applied at rates corresponding to 70 kg NH₄⁺-N ha⁻¹ on bacterial community structure and microbial activity in three soils of different texture (a sandy, a clay and an organic clay soil). 16S rRNA genes were targeted in PCR reactions and bacterial community profiles visualized using terminal restriction fragment length polymorphism. General microbial activity was measured as basal respiration (B-resp), substrate-induced respiration (SIR), specific growth rate (μ_SIR), metabolic quotient (qCO₂) and nitrogen mineralization capacity (NMC). Non-metric multidimensional scaling analysis visualized shifts in bacterial community structure related to microbial functions. There were significant differences in bacterial community structure after 120 days of incubation (+20 °C at 70% of WHC) between non-amended (control) and amended soils, especially in the sandy soil, where CS caused a more pronounced shift than biogas residues. Terminal-restriction fragment (TRF) 307, the predominant peak in CS-amended sandy soil, was identified as possibly Bacillus or Streptococcus. TRF 226, the dominant peak in organic soil amended with BR-B, was classified as Rhodopseudomonas. B-resp significantly increased and SIR decreased in all amendments to organic soil compared with the control, potentially indicating decreased efficiency of heterotrophic microorganisms to convert organic carbon into microbial biomass. This was also reflected in an elevated qCO₂ in the organic soil. The μ_SIR level was higher in the sandy soil amended with BR-A than with BR-B or CS, indicating a shift toward species capable of rapidly utilizing glucose. NMC was significantly elevated in the clay and organic soils amended with BR-A and BR-B and in the sandy soil amended with BR-B and CS. Thus, biogas residues and cattle slurry had different effects on the bacterial community structure and microbial activity in the three soils. However, the effects of biogas residues on microbial activities were comparable in magnitude to those of cattle slurry and the bacterial community structure was less affected. Therefore, we do not see any reason not to recommend using biogas residues as fertilizers based on the results presented.     

Changes on carbohydrates and nitrogen content in the bark tissues induced by artificial chilling and its relationship with dormancy bud break in Prunus sp.

Stem cuttings of peach, nectarine, plum and apricot, obtained from 1-year-old shoots at leaf drop in autumn, were exposed at a constant 3.0 ± 0.1 °C to simulate different chilling accumulation treatments (0, 500, and 1000 chilling hours). A set of 10 excised shoots was forced to budbreak after chilling accumulation; a second set of 10 excised shoots was used to analyse sugars, starch, and ammonium-, nitric- and proteinaceous-nitrogen content in the bark tissues. Sorbitol, fructose and glucose were the main sugars in the bark tissues, whereas sucrose and rafinose showed the lowest quantities in all Prunus sp. studied. Sorbitol and starch concentration decreased whereas glucose and fructose concentration increased with chilling. Ammonium-nitrogen showed the more dramatic increase with medium chilling treatment, decreasing again at high chilling exposure. No modifications in proteinaceous- and nitric-nitrogen levels were observed due to chilling. Chilling treatments increasing flowering intensity showed high ammonium- and proteinaceous-nitrogen content in the bark tissues of the stem cuttings, whereas those reducing flowering intensity showed high starch and nitric-nitrogen content, in three of the four Prunus sp. studied. Ammonium-nitrogen content gives the highest correlation with flowering intensity.     

MEK-石脑油可回收稀释降粘输送工艺

概述了稠油的物性,分析了石脑油中加入MEK(甲基乙基酮)后对稠油的稀释降粘效果。介绍了MEK-石脑油可回收的稠油稀释降粘工艺流程,指出MEK-石脑油回收稀释降粘工艺虽比石脑油回收稀释降粘工艺增设了一套MEK回收装置,投资及运行成本略高,但其降粘效果较好,能够以较小的稀释比提高稠油的输送能力。     

Water management and crop production for food security in China: A review

Food security is a high priority issue on the Chinese political agenda. China’s food security is challenged by several anthropogenic, sociopolitical and policy factors, including: population growth; urbanization and industrialization; land use changes and water scarcity; income growth and nutritional transition; and turbulence in global energy and food markets. Sustained growth in agricultural productivity and stable relations with global food suppliers are the twin anchors of food security. Shortfalls in domestic food production can take their toll on international food markets. Turbulence in global energy markets can affect food prices and supply costs, affecting food security and poverty. Policy safeguards are needed to shield food supply against such forces. China must make unremitting policy responses to address the loss of its fertile land for true progress towards the goal of national food security, by investing in infrastructure such as irrigation, drainage, storage, transport, and agricultural research and institutional reforms such as tenure security and land market liberalization. The links between water and other development-related sectors such as population, energy, food, and environment, and the interactions among them require reckoning, as they together will determine future food security and poverty reduction in China. Climate change is creating a new level of uncertainty in water governance, requiring accelerated research to avoid water-related stresses.     

细胞生物学实验教学中“节能减排”方法的探讨

细胞生物学实验对有毒、有害试剂及材料的使用量大,产生实验垃圾量大,是教学实验室中的污染大户。为在实验教学中做到＂节能减排＂,采取了以下措施：将环保素质教育引入实验教学中;制定有效管理考查措施,强化学生在实验中的环保意识;在实验设计、组配、准备和学生实施操作等的每个细节中做到精心设计,少用少排。多年的实践表明这种方法行之有效,不仅可减少细胞生物学实验中对药品、水电等的消耗,同时还可大大减少实验垃圾的产生量。     

添加剂湍流减阻流动的传热特性数值计算

采用雷诺应力模型和Cross粘度方程,对二维轴对称圆管内湍流流动的聚丙烯酰胺稀溶液（PAM）的流场和温度场进行了数值模拟.在数值计算结果的基础上,系统研究了流速对减阻流体的减阻性能和传热特性的影响.结果表明,减阻流体的减阻率和传热降低率随流速的增大逐渐增大到渐进值,而且传热降低率恒大于减阻率.减阻流体柯尔本（Colburn）因子与摩擦系数的对比结果表明柯尔本比拟,特别是在较低流动雷诺数下有较大偏差.     

马铃薯氮素营养诊断研究进展

从外观诊断、化学诊断、无损测试技术3个方面,总结了目前马铃薯氮素营养诊断的若干方法.对各种方法的应用原理、发展现状进行了阐述,同时,对其他可用于马铃薯氮素营养诊断,但目前仍未采用的方法进行了简要的说明和论述.     

浅谈蓝沙还原卡V9.2在高校电子阅览室的应用

阐述了蓝沙还原卡v9.2在保护电子阅览室计算机系统方面上的优势、工作原理、以及使用中常遇到的问题和解决方法。     

不同碳源、氮源对香魏菇菌丝体生长的影响测定

以菌丝体生物量为指标,对香魏菇液体培养基中碳氮营养源进行了优化。结果表明香魏菇菌株能利用多种碳源、氮源,其中碳源以葡萄糖较好;有机氮源优于无机氮源,牛肉膏为较佳氮源。     

砂土中粘土夹层对入渗过程影响的试验分析

对水在高渗透性砂内入渗过程中,其内部的低渗透性粘土夹层对入渗过程的影响进行了室内垂直一维入渗试验。通过分析定性得出粘土夹层对入渗过程的影响体现为减渗作用,表现为粘土夹层将均质粗砂中的非线性入渗过程变为夹层内的线性入渗过程,使夹层范围的入渗为一个具有较小入渗率和较小湿润锋推进速度的稳渗阶段。