抗生素工业化生产中一级种子罐染菌原因及解决方法

抗生素工业化生产中,菌种往往需要扩大繁殖到一定的数量后再进入发酵罐.在一级种子制备过程中,引起染菌的因素很多,极大地影响了抗生素生产的产量和质量.解决一级种子罐染菌的方法,除制定科学的岗位SOP并不断提高操作者的技能外,更重要的是要加强工艺管理,树立以预防为主的思想,根据微生物特性,强化环境清洁,加强设备清洁、维修,操作时实行定人、定操作.     

Reliably colonising broiler chickens with Campylobacter spp. using a litter-based method

ABSTRACT

1. Chicken-associated Campylobacter spp. are the cause of most food poisoning cases in Europe. In order to study the host–pathogen interactions, a reliable and reproducible method of colonising chickens with the bacteria is required.

2. This study aimed to identify a more appropriate and less invasive method of colonisation (cf. gavaging) by seeding bedding material (litter) that commercial chickens are kept on with a mixture of Campylobacter spp., broth and faeces.

3. The first phase of the study tested the longevity of Campylobacter spp. recovery in seeded litter over 24 h: significantly more Campylobacter spp. was recovered at 0 or 3 h post-seeding than at 6 and 24 h post-seeding, indicating that the pathogen can survive to detectable levels for at least 3 h in this environment.

4. In the second phase, three groups of 10 broiler chickens (negative for Campylobacter spp. prior to exposure) were exposed at 21 days of age to one of three different Campylobacter jejuni and C. coli mixes (A, B, C), using the method above. At 28 days of age, birds were euthanised by overdose of barbiturate or cervical dislocation, and livers and caeca removed for Campylobacter spp. assessment.

5. All liver and 28/30 caeca samples tested positive for Campylobacter spp., with mix A and C giving higher counts in the caeca than mix B. The method of euthanasia did not affect Campylobacter spp. counts.

6. In conclusion, a successful method for reliably colonising broiler chickens with Campylobacter spp. has been developed which negates the need for gavaging and is more representative of how contamination occurs in the field.     

密闭式猪舍环境监控系统的发展研究

控制系统是密闭式猪舍环境调控的关键组成部分.该研究系统归纳分析了应用于密闭式猪舍环境调控的4种控制系统,包括基于单片机控制系统、基于PLC控制系统、基于CAN总线的分布式控制系统和基于物联网的监控系统,总结出了各自的结构特点.结合工业控制技术、计算机技术和网络通讯技术的发展,提出了密闭式猪舍环境控制系统的发展趋势,对密闭式猪舍环境控制系统的设计具有一定的参考价值.     

微生物燃料电池在环境监测中的研究进展

微生物燃料电池在环境监测领域有巨大的应用潜力.概述微生物燃料电池应用于环境监测的基本原理,对目前已有的微生物燃料电池型传感器为监测方法进行详细的介绍,包括微生物燃料电池用于易降解碳源、有毒污染物及微生物数量检测三方面的研究.最后探讨了微生物燃料电池传感器目前还未被广泛应用于实际水质监测的原因,以期为未来研究开发高性能微生物燃料电池传感器提供理论参考依据.     

环境地学课程教学改革的探索

人类对环境的影响越来越大,环境问题日益突出,社会对环境专业人才需求旺盛,而环境地学课程是环境科学的专业基础课,部分教学内容及教学方法已不能跟上环境科学知识的前沿,有必要对环境地学课程体系进行改革。该文提出了环境地学课程改革的对策,包括教材的选择、教学内容的安排、教学实习、多媒体技术与信息技术的利用等,使环境地学的课程更加有利于创新型人才的培养。     

浅议湿地公园环境教育体系之建设架构

通过分析湿地公园环境教育的目标和特点,进而得出环境教育的建设构架可分为环境解说、博览展示、参与体验三个方面,同时,通过不断对比各种教育受众特点等方法,得出结论:在湿地公园中开展环境教育是基于完善的科普宣教场所、完整的环境解说系统和合理的组织形式三者完美结合而实现的。     

基于B-S模型的环保项目投资评价

以ABC公司环保产品开发为例,针对传统项目投资评价在环保项目上的缺点,分析了将实物期权理论运用到环保项目投资评价中的可行性,并运用B-S模型进行定量分析,以期为环保项目投资提供一个新的思路,同时也使环保项目决策变得更加合理。     

Simultaneous detection of multiple mycotoxins in broiler feeds using a liquid chromatography tandem-mass spectrometry

Mycotoxins are secondary fungal metabolites that are typically present in grain and feed ingredients used for animal feeds. An analytical method using LC-ESI-MS/MS was developed to quantify nine mycotoxins, consisting of aflatoxin B₁ (AFB₁), AFB₂, AFG₁, AFG₂, T-2 toxin, deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEA) and ochratoxin A (OTA) in broiler feeds. In total, 100 samples of broiler feeds were collected from poultry farms in Central Thailand. The survey found that AFB₁ and ZEA were the most prevalent mycotoxins in the feed samples at percentages of 93% and 63%, respectively. The limit of detections (LODs) of investigated mycotoxins was 0.20–0.78 ng/g. AFB₂, DON, AFG₁, NIV and T-2 toxin were also detectable at low contamination levels with percentages of 20%, 9%, 7%, 5% and 1%, respectively, whereas OTA and AFG₂ were not detected in any of the feed samples. These results suggest that there is a very low level of risk of the exposure to mycotoxins in feeds obtained from broiler farms in Central Thailand.     

沿滩区环境监测能力现状与建议

随着社会经济的快速发展,环境保护越来越被人们所重视.环境保护部门肩负着重要的使命和神圣的职责,而环境监测又是环境保护的关键环节,它将为搞好环境保护工作提供有效的技术支持与服务.因此,环境监测能力的高低势必会影响监测质量的好坏.分析了沿滩区环境监测能力的现状和存在的问题,提出了相关建议,使环境监测为环境保护工作提供有效的技术支持与服务.