Atorvastatin inhibits IL-1β and IL-18 releases via lowering the expression of NLRP1 inflammasome

AIM: To explore the role of nucleotide-binding oligomerization domain-like receptor protein 1 (NLRP1) inflammasome in atorvastatin-induced reduction of interleukin-1β (IL-1β) and interleukin-18 (IL-18) releases from the THP-1 macrophages. METHODS: Lipopolysaccharide (LPS, 10 μg/L) was used to trigger the secretion of IL-1β and IL-18 in the THP-1 macrophages. The cells were incubated with different concentrations of atorvastatin (1, 10 and 20 μmol/L) for 24 h, or treated with 10 μmol/L atorvastatin for different time (12 h, 24 h and 48 h). NLRP1 siRNA was transfected into the THP-1 cells. The mRNA expression of NLRP1 inflammasome was detected by RT-PCR. The protein expression of NLRP1 inflammasome was determined by Western blot. The secretion of proinflammatory cytokines IL-1β and IL-18 was quantified by ELISA. RESULTS: Atorvastatin inhibited the mRNA and protein expression of NLRP1 inflammasome in the THP-1 macrophages in a dose- and time-dependent manner. Transfection of NLRP1 siRNA significantly decreased the protein expression of NLRP1 and promoted the suppressive effect of atorvastatin on IL-1β and IL-18 secretion in the THP-1 macrophages. CONCLUSION: Atorvastatin inhibits the production of IL-1β and IL-18 in the macrophages through decreasing NLRP1 inflammasome expression, possibly contributing to the anti-inflammatory effect of atorvastatin on atherosclerosis.     

进境美国大豆杂草和杂质含量研究

自装载美国大豆的外轮货舱中取样,研究进口美国大豆携带杂草和杂质的含量关系:取样量为5 kg/1 000 t,是能反映进口大豆中杂草和杂质含量关系的最低取样量;把进口美国大豆杂质含量划分为1.5%、1.5%~2.0%和2.0%3个区间,其携带杂草含量和种类与杂质含量呈显著的正相关;进口大豆中携带杂草含量高的,检出频率也相对高,但检出的杂草总种类数少于杂草含量低的;杂草含量低的,检出频率也相对低,但检出的杂草总种类数多于杂草含量高的。     

辣椒新品种航椒18号的选育

航椒18号是以051-3-2-2-H-H-H为母本,以035-1-2-2-H-H为父本杂交育成的早中熟羊角型辣椒一代杂种。从定植至青熟果采收55d(天)左右,始花节位为第9~11节。果面皱、果肩宽、有光泽,味香辣;单株结果数20个左右,单果质量50.5g左右;果实粗长羊角形,纵径22.7cm,横径4.2cm,肉厚0.25cm。田间对辣椒病毒病、炭疽病和疫病的抗性强于对照陇椒3号。平均每667m~2产量4200kg,适宜在甘肃、内蒙古、新疆等地及生态环境相似区域保护地栽培。     

试论我国科技期刊发展的集团化趋势

在分析我国科技期刊发展的现状的基础上,提出了集团化是科技期刊发展的必然趋势。     

Classification of Tomicus Species

Both morphological characters and a portion of of 28S rDNA sequences were used to identifying Tomicus species.The specimens were classed into groups with the following characters: 1) granules or punctures on interstria 2 on the declivity of the elytra;2) length of the elytral interstrial hairs and hairs arising from punctures;3) arrangement of pronotal punctures and hairs.These characters could be clearly examined under a binocular microscope at 30×magnification and they were applicable and valuable for the forest entomologists to identify Tomicus species.The phylogenetic tree established with difference in 28S rDNA sequence of D2 region revealed that the specimens of each group identified by morphological characters were also grouped together.The genetic distances of intra-species, inter-species and inter-genus were not overlapped. Genetic divergence of 28S rDNA was also useful for identifying Tomicus species.     

优质籼型水稻光温敏核不育系万金S的选育

万金S是广东海洋大学农学院利用扬稻6号(9311)的育性变异单株育成的水稻光温敏核不育系,于2009年9月通过广东省技术鉴定。万金S不育期间败育彻底,不育性稳定,不育株率100%,花粉不育度和套袋自交不实率均为99.99%;不育起点温度较低(23～24℃),在湛江不育期135 d以上;开花习性好,异交结实率高达60%以上;米质优,达国标《优质稻谷》2级标准;所配组合杂种优势强,稻米品质优,其中万两优香1号于2011年6月通过了海南省品种审定。     

PLC特性及其在碱回收系统中的应用设计

介绍了s7—400PLC的主要特性以及该系统的实现，重点给出了系统的下位机硬件和软件设计，并结合佳木斯造纸厂碱回收系统的设计讨论了PLC的应用。     

Temporal changes of soil physical quality under two residue management systems

Although crop residue management is known to affect near‐surface soil physical quality, little is known about the temporal variability of these indicators over short time intervals. This study evaluates the temporal changes of nine indicators of soil physical quality. These are organic carbon content, structural stability index, bulk density, macroporosity, air capacity, relative field capacity, plant available water capacity, Dexter's S‐index and saturated hydraulic conductivity. A second set of soil physical indicators, based on the distribution of soil pore volume, was also evaluated. The indicators were determined in three different times during the growing cycle of winter durum wheat cultivated within a long‐term field research carrying out in Southern Italy and comparing two types of crop residue management, that is, burning (B) and soil incorporation (I). Only the bulk density changed over time for both treatments, although the air capacity also changed for the incorporation of wheat residues. Residual effects of the autumnal soil tillage and soil compaction were a common source of variability, irrespective of which treatment was used. Based on the existing guidelines for evaluating the physical quality of these agricultural soils, optimal or near‐optimal values were detected in about half of the cases under consideration. This suggests that both B and I create sufficiently good conditions for crop growth during the crop cycle. The comparison between observed and optimal soil pore distribution function was always poor. The pore volume distributions showed lower densities of small pores and relatively higher densities of large pores than the proposed optimal distribution. This study also suggests that the considered optimal or references curves probably cannot be applied successfully to a wide range of agricultural soils.     

基于18S rRNA的鸡组织滴虫病PCR诊断方法的建立

河南某鸡场约4周龄鸡疑似发生鸡组织滴虫病.根据鸡组织滴虫18 S rRNA序列设计引物,提取肝脏、盲肠内客物寄生虫DNA,采用PCR方法检测.结果表明,PCR扩增到与预期大小一致的产物,经测序比对,证实为鸡组织滴虫感染.所建立的PCR方法具有灵敏、特异、快速等优点,不仅能用于鸡组织滴虫病临床诊断,还能用于开展流行病学研...