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81.
YUE Yuan CHEN Hong-yan WANG Jia-wei XU Ming-qiang DING Yu JIANG Hao GAO Yan ZHANG Jia-bao YAN Shou-qing 《中国畜牧兽医》2016,43(3):585-591
The aim of this study was to investigate the differential expression genes induced by ApoCⅢ,and study the function of ApoCⅢ.Porcine aortic vascular endothelial cells were successfully isolated using enzyme digestion,and then screened the differential expression genes induced by ApoCⅢ using the Solexa high-throughput sequencing technology.The results showed 647 differential expression genes,including 390 up-regulated genes and 257 down-regulated genes.The qRT-PCR results verified that the gene expression results from Solexa sequencing data were reliable.GO and Pathway analysis showed that the function of differential expression genes were related to immune response,cell apoptosis and death.These findings suggested that ApoCⅢ affected the physiological function of porcine aortic endothelial cells by the molecular pathways of inflammation,cell adhesion and apoptosis,which provided a theoretical basis for further understanding the molecular mechanisms of atherosclerosis caused by ApoCⅢ. 相似文献
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Background
Invasive Australian populations of redlegged earth mite, Halotydeus destructor (Tucker), are evolving increasing organophosphate resistance. In addition to the canonical ace gene, the target gene of organophosphates, the H. destructor genome contains many radiated ace-like genes that vary in copy number and amino acid sequence. In this work, we characterise copy number and target-site mutation variation at the canonical ace and ace-like genes and test for potential associations with organophosphate insensitivity. This was achieved through comparisons of whole-genome pool-seq data from alive and dead mites following organophosphate exposure.Results
A combination of increased copy number and target-site mutations at the canonical ace was associated with organophosphate insensitivity in H. destructor. Resistant populations were segregating for G119S, A201S, F331Y at the canonical ace. A subset of populations also had copy numbers of canonical ace > 2, which potentially helps overexpress proteins carrying these target-site mutations. Haplotypes possessing different copy numbers and target-site mutations of the canonical ace gene may be under selection across H. destructor populations. We also detected some evidence that increases in copy number of radiated ace-like genes are associated with organophosphate insensitivity, which might suggest potential roles in sequestration or breakdown of organophosphates.Conclusion
Different combinations of target-site mutations and (or) copy number variation in the canonical ace and ace-like genes may provide non-convergent ways for H. destructor to respond to organophosphate selection. However, these changes may only play a partial role in organophosphate insensitivity, which appears to have a polygenic architecture. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献84.
Engineering designed mode has entered a new stage because of the development of network. New calculating system based on network appeared. By analyzing the common B/S architecture, this paper brings forward an reformative architecture based on Web services and ASP. NET in order to build the new mode of engineering calculation. It can fully implement the sharing of the resources and provide the engineering designers a wonderful calculating environment, and the working eifficiency of designers can be enhanced. 相似文献
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早籼型水稻温敏核不育系M103S的选育与光温反应特性 总被引:3,自引:2,他引:3
以温敏核不育水稻安农S—1为母本,以常规高产早籼稻品系92153为父本进行杂交,通过低温选择和人工气候箱鉴定,育成了育性转换临界温度23—24℃的早籼型温敏核不育系M103S。光周期长于12.5h,日平均温度高于24℃,可以用于制种,日平均温度低于23℃,可以用于繁殖。光周期长于13.5h,日平均温度高于23℃,可以用于制种。自然条件下不育起点温度为日均温23—24℃,耐低温期为3—4d。敏感期为抽穗前的7—14d。播始历期为53—79d,主茎叶片数10.0—12.6片。从播种到始穗的活动积温和有效积温,分别为1493.8—1766.3℃和733.4—1058.3℃。不育期的株高63.6—77.5cm,平均72.0cm,单株有效穗6.4—12.0个,平均9.3个,穗长20.9—22.7cm,平均21.2cm,每穗颖花数112.1—137.3朵,平均122.3朵。可育期株高65.7cm,单株有效穗7.2个,穗长17.2cm,每穗总粒数114.1粒,结实率47.23%,千粒重31.34g。以M103S为母本,与100多个早籼品种进行测配,杂种表现生育期早、穗大、粒多、千粒重高。 相似文献
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提出以数据仓库、方法库、模型库、知识库一体化模式实现基于“3S”的新疆融雪洪水预警决策支持系统的研究思路。信息系统是整个系统的事务管理层,决策支持系统则是系统应用处理层,两者互为依托。信息系统是决策支持系统的信息源,决策支持系统所产生的结果进入信息系统并对其进行管理,当决策支持系统的模型、方法、知识运作成熟,数据结构化后,又将进入信息系统中,成为信息系统的组成部分。 相似文献
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