Development of an indirect enzyme-linked immunosorbent assay for detecting equine serum antibodies to the lipopolysaccharide of Salmonella abortusequi

An indirect enzyme-linked immunosorbent assay (IELISA) was developed for the detection of equine serum antibodies to lipopolysaccharide of Salmonella enterica subsp. enterica serovar Abortusequi (LPS), a causative organism of Equine Paratyphoid. The data presented demonstrates that horses immunized with S. abortusequi LPS developed antibodies detectable by the IELISA. By comparison, the tube agglutination test (TAT) did not detect antibody to S. abortusequi LPS as consistently as the IELISA. The data suggests that the IELISA may be a more suitable test for the detection of serum antibodies to S. abortusequi than the TAT.     

Effect of terbutryn at environmental concentrations on early life stages of common carp (Cyprinus carpio L.)

The aim of this study was to assess the toxicity of terbutryn to early developmental stages of common carp (Cyprinus carpio). Based on accumulated mortality in the experimental groups, lethal concentrations of terbutryn were estimated at 36 day LC50 = 3.06 mg l⁻¹ terbutryn. Based on inhibition of growth in the experimental groups, lowest observed-effect concentration (LOEC) = 0.005 mg l⁻¹ terbutryn; and no observed-effect concentration (NOEC) = 0.0007 mg l⁻¹ terbutryn. Fulton’s condition factors were significantly lower in fish exposed to 2 mg l⁻¹ compared with controls. By day 30, fish exposed to 0.00002 mg l⁻¹ - real environmental concentration in Czech rivers - 0.02, 0.2, and 2 mg l⁻¹ terbutryn showed significantly lower mass and total length compared with controls. No significant negative effects on hatching or embryo viability were demonstrated at the concentrations tested, but significant differences in early ontogeny among groups were noted. Fish from the two highest tested concentrations (0.2 and 2 mg l⁻¹) showed a dose-related delay in development compared with the controls. At concentrations of 0.02, 0.2, and 2 mg l⁻¹ damage to caudal kidney tubules when compared to control fish was found.     

长武县“耕地—果园”数量变动与经济发展关系分析

利用1985—2008时间、空间序列数据,采用趋势分析、协整分析和Granger因果分析和区位熵评价法分析长武县耕地、果园数量与经济发展之间的关系。研究时段内,可得到果业面积和经济发展的长期均衡模型和短期波动的误差修正模型;由于人均耕地面积为非稳定序列,Granger因果检验可探究耕地数量与经济发展关系,其结果显示长武县的经济发展并非以耕地为代价,经济发展对耕地面积影响不大。此外,从空间角度探讨长武县果业发展和耕地减少之间的关系,区位熵评价结果说明各乡镇果业发展与耕地面积减少的协调度不同,存在空间差异。     

近50 a甘肃省夏季日极端降水频数与强度变化特征

基于甘肃省近50 a夏季日降水量数据,借助ArcGIS 9.2和Matlab 7.0数据软件平台,运用复值Morlet小波分析及Mann kendall检验法,分析甘肃省夏季日极端降水频数和强度的周期及空间分布特征。结果表明：① 极端降水频数主要存在19 a和8 a周期,极端降水强度主要存在11 a和19 a周期;② 夏季极端降水量与夏季降水量的空间分布在东南部地区有明显的差异;③ 极端降水频数呈减少趋势,而极端降水强度呈增加趋势。     

1960-2009年大连市气候暖干化研究

利用大连市1960-2009年气温和降水两个主要气象要素值的基本观测资料,运用线性倾向估计和GIS空间分析方法,探讨了大连气候的年际时空变化,同时利用Mann-Kendall统计检验和Morlet小波分析对其气候的年代际和周期性变化进行了分析。结果表明:近50a来大连市气候暖干化日趋明显,年平均气温以0.28℃/10a的速率显著上升,并且在1991年发生突变进入异常增暖期,空间上增暖趋势大致呈由南向东北逐渐减弱的态势;降水量基本经历了"减少-增加-减少-增加"的变化过程,总体上呈下降趋势,在1972年降水量由偏多向偏少转变,东北和西南地区降幅较大,腹地最小。此外,气候的暖干化趋势对"自然-社会-经济"复合系统造成的影响应引起重视。     

玉米子粒植酸酶活性测定方法研究

对转植酸酶基因玉米子粒中植酸酶活性检测方法中的影响因素进行了研究和优化.正交试验结果表明:底物浓度0.1 mmol/L,pH 5.5,反应时间30 min为最佳测定条件.     

2012年棉花“3414”试验总结

通过新疆泽普县棉花“3414”田间肥料试验,获得县棉花作物的最佳施肥量,施肥比例,施肥时期,施肥方法,筛选验证土壤养分测试技术,建立施肥指标体系.建构泽普县棉花施肥模型,为施肥分区和肥料配方提供依据.     

5.00%唑啉草酯EC防治大麦田禾本科杂草试验

介绍5.00%唑啉草酯EC防治大麦田禾本科杂草试验及结果。     

沼液在冬春茬日光温室番茄上的应用效果分析

沼液作为优质液肥,营养成分可直接被农作物吸收,参与光合作用。作为叶面追肥,确实具有增产增效、提高品质、减少农药施用的作用,从而增加产量提高品质。     

剑河县革东镇水稻测土配方施肥“3414”试验结果分析

通过对剑河县革东镇前进村氮、磷、钾"3414."的肥效试验,得出水稻生产中氮、磷、钾三元素的合理施用量,取得水稻肥效的最佳施肥量,为当地农民科学施肥提供依据。