吸湿—回干处理提高大豆种子活力,增强抗逆力的效果

黑河3号和黑河5号大豆种子经吸湿—回干处理后,活力明显提高。回干后的种子具有明显的抗吸胀冷害的效果,经低温吸胀后,其种子比对照种子的萌发率提高1.6倍,电导率及K~+/Na~+明显降低。     

外源褪黑素对低温胁迫下菜豆种子萌发及抗性的影响

为了探究褪黑素(Mt)对低温环境下菜豆种子的影响,本研究对菜豆种子进行低温处理(LT)和低温加褪黑素的处理(LT+Mt),并设置常温处理作为对照(CK),对同时期的各项生理指标进行测定。结果表明：Mt可以提高低温胁迫下的种子萌发率,但萌发率要低于对照;同时对渗透调节物质、O₂^-和MDA、抗氧化酶活性进行测定,发现低温增加了渗透调节物质的含量,当加入Mt后其含量进一步增加;低温处理后O₂^-和MDA的含量增加,当加入Mt后其含量明显降低,但是要高于对照;低温处理后抗氧化酶活性略低于对照,当加入Mt后其含量显著升高。研究表明外源Mt有效的减轻了低温对种子萌发的损害,从而促进种子萌发过程中抵御低温的能力。     

大鼠膝关节前交叉韧带离断后关节软骨损伤的观察

目的观察大鼠膝关节前交叉韧带离断术后关节软骨形态学变化。方法 56只SD大鼠随机分为7组,每组8例,前交叉韧带离断术后当天及术后2、4、8、12、16、20周处死大鼠,用Pelletier评分及HE染色观察膝关节软骨损2伤情况。结果 Pelletier评分显示软骨损伤随造模时间延长而加重（χ=45.861,P〈0.05）,二者呈线性相关（F=178.836,P〈0.05）;髁上部软骨损伤比下部明显（P=0.000）,而内髁与外髁软骨损伤差异无统计学意义（P=0.736）。HE染色发现造模后软骨面裂隙形成、软骨细胞排列紊乱、潮线部分破坏。结论前交叉韧带离断后关节软骨损伤随时间推移而加重,其中髁上部软骨损伤程度较重。     

黄淮南片麦区小麦倒春寒冻害成因及预防措施

倒春寒冻害是目前影响黄淮南片麦区小麦高产稳产的主要障碍因子之一.为此,分析了黄淮南片麦区小麦倒春寒的成因,提出了相应措施.该区小麦倒春寒发生的时间多在3月中下旬至4月上中旬.引起倒春寒冻害的主要原因有春季干旱、极端冷暖异常天气交替频繁、耕种和栽培管理措施不当、品种耐寒性及耐旱性偏弱等.合理选用小麦品种、科学耕种和栽培管理、春季适时水肥管理、冻害发生后及时追肥浇水是预防和减轻倒春寒危害的有效措施.     

不同立地对麻疯树苗木周年生长的影响

以麻疯树栽培品种FD-8 号为试材,采用盆栽方式,将其置于露地（T1）、林间地（T2）及遮雨棚（T3）3 种 条件下,研究立地对麻疯树周年生长的影响。结果表明,秋季子叶灼伤和冬季苗木冷害均表现为T1 最严重,其次是 T2,而T3 没有出现子叶灼伤和苗木冷害;在春季,T3 萌芽最早,其次是T2,而T1 萌芽最晚;在秋季和夏季,T2 均明 显促进苗木形态协调生长,显著提高苗木质量指数,但T3 显著降低苗木综合质量。可见,本试验条件下,T2 有利于促 进麻疯树苗木形态的周年协调生长,从而提高苗木综合质量。     

枸杞多糖对四氯化碳诱导建鲤原代肝细胞损伤的保护作用研究

研究中药枸杞多糖(Lycium barbarum polysaccharide,LBP)对四氯化碳(CCl4)诱导的建鲤(Cyprinus carpiovar Jian)肝细胞急性损伤的保护作用。利用8 mmol/L四氯化碳构建建鲤肝细胞损伤模型,用不同浓度的枸杞多糖处理肝细胞,设空白对照组、枸杞多糖对照组(0.4 mg/mL)、模型组(CCl4)、预防组(CCl4处理前加枸杞多糖孵育,0.1、0.2和0.4 mg/mL)、治疗组(CCl4处理后加枸杞多糖孵育,0.1、0.2和0.4 mg/mL)和预防治疗组(枸杞多糖孵育后经CCl4处理再经枸杞多糖孵育,0.1、0.2和0.4 mg/mL)。12 h后,收集细胞和细胞培养液,然后测定谷丙转氨酶(GPT)、谷草转氨酶(GOT)、乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)、细胞活力、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、免疫球蛋白M(IgM)及药物代谢酶细胞色素P450 2E1(CYP2E1)等生化指标。研究结果表明:CCl4处理后可以显著增加GPT、GOT、LDH、MDA、TNF-α、IL-1β、IgM及CYP2E1的含量,降低SOD活性和细胞活力;枸杞多糖处理组可不同程度地抑制上述生化指标的变化,其中预防组及预防治疗组对于转氨酶(GPT、GOT)、LDH、MDA、细胞因子(TNF-α、IL-1β)、IgM及CYP2E1等活性的升高皆有显著抑制作用,同时显著提高SOD酶活性及细胞活力,且呈剂量依赖性;而治疗组仅对LDH、TNF-α及CYP2E1有显著的抑制作用。综上结果,认为枸杞多糖对四氯化碳致建鲤肝损伤具有一定保护作用,可以应用于鱼类肝损伤的防治。     

外源GA3与低温处理对芍药种子萌发和幼苗生长的影响

芍药种子具有“双休眠”特性,自然播种萌发率低,生长周期长。试验对‘朱砂判’种子进行了GA₃和低温处理,研究其对促进种子萌发和幼苗生长的效果。结果表明,GA₃能够解除种子下胚轴休眠并促进生根,其中100 mg·L^-1GA₃处理的生根率与生根质量最好,生根率最高为41.33%;低温结合GA₃处理不同主根长度的种子,对解除上胚轴休眠和促进幼苗生长均具有显著性影响,当主根长度＜3 cm时,低温（4℃）4 周后500 mg·L^-1GA₃的处理最有利于种子萌芽与幼苗生长,萌芽率最高为96.67%;这种组合处理还可以促使芍药种子从播种到萌发所需时间缩至48 d。     

Willingness of a Convenience Sample of Horse Owners to Use Stem Cell Therapy for the Treatment of Equine Tendon Injury and Factors that Influence This Decision

Stem cell therapies offer huge potential for the treatment of tendon injury in horses. However, the factors influencing clients' willingness to use these therapies have not yet been investigated. This study examines the willingness of a population of horse owners to choose stem cell therapy for the treatment of equine tendon injuries and the factors that may influence this choice including treatment cost and efficacy, veterinarian advice, client knowledge, and success stories. A short, anonymous questionnaire was delivered to a convenience sample of equine owners. Questions related to (1) respondent background; (2) prior experience with tendon injuries and their treatment; (3) willingness to use equine stem cell therapy; and (4) the importance of factors that may affect their decision to use the therapy. Responses were summarized using graphical methods and compared using Fisher exact test where appropriate. Fifty-four percent of our cohort (n = 113) reported they would be willing to use equine stem cell therapy, 6% were not willing, and 40% were unsure. When asked to rank factors that would influence their decision, scientific proof that the therapy works was ranked highest followed by veterinarian advice. This result may have been influenced by the high proportion of tertiary-educated respondents recruited in this study. This is the first reported study to investigate the willingness of a population of horse owners to use stem cell therapy for their horses. Results suggest that the majority of these equine owners may consider using stem cell therapies for their horses given a larger positive evidence base was provided for the therapy.