Identification of polymorphisms and association analysis with meat quality traits in the porcine KIAA1717 and HUMMLC2B genes

Skeletal muscle genes are potential candidates for production and meat quality. Screening a subtracted cDNA library constructed with mRNA obtained from longissimus dorsi muscles of F1 hybrids Landrace × Yorkshire and their female parents Yorkshire, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. Database search revealed KIAA1717 and HUMMLC2B encoded proteins with SET domain and EF-hand calcium binding motif, respectively. In the present work we identified their partial polymorphisms and two SNPs, one (C1354T) at the 3′ untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, both created/disrupted a restriction site for endonuclease Msp I. The selected pigs were genotyped at the KIAA1717 C1354T and HUMMLC2B A345G sites by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with meat marbling (longissimus doris (p < 0.05), biceps femoris (p < 0.01)) and intramuscular fat (p < 0.01). HUMMLC2B A345G were significantly associated with meat pH (longissimus doris (p < 0.05), biceps femoris (p < 0.01)), drip loss (p < 0.01), water holding capacity (p < 0.01) and meat color value (longissimus doris (p < 0.01), biceps femoris (p < 0.05)). Further studies are needed to confirm these preliminary results.     

单色光对蛋白高度、哈氏单位和蛋黄颜色的影响

180只海兰褐蛋鸡分别饲养在蓝、绿、红和白等不同光环境中,试验期为19～45周龄,光照强度为15lx,光照时间为16h。试验结果显示,绿光组蛋白高度最高((7.13±0.04)mm),与白光和蓝光组差异显著(P<0.05);绿光和红光组哈氏单位显著大于白光和蓝光组(P<0.05);白光组蛋黄颜色值(4.6±0.15)最大,与其它组之间差异极显著(P<0.01),但绿光组蛋黄颜色值与周龄间相关系数(r)极显著(P<0.01)。结果表明单色光可影响蛋品质,其中绿光组蛋品质最好。     

Effects of Cage Location and Tier Level with Respect to Light Intensity in Semiconfined Housing on Egg Production and Quality During the Late Laying Period

This experiment was conducted to determine the effects of cage location and tier level with respect to light intensity on egg production and egg quality of hens housed in a semiconfined facility. Hens (ISA Brown, n = 225) at 75 wk of age were placed into 3-tier cages as top (T), middle (M), and bottom (B) tiers located in cages illuminated artificially (EI), by window (FW), or between corridors (C) for 2 mo. Light intensity was measured monthly for each cage at 5 cm from feeders every 6 h. Egg production was recorded daily and egg quality was assessed biweekly. Light intensity was the greatest for cages in the FW group (151.9, 119.8, and 89.8 lx for tiers T, M, and B, respectively), followed by EI (52.6, 54.5, and 51.0 lx for tiers T, M, and B, respectively), and C (44.5, 23.4, and 4.7 lx for tiers T, M, and B, respectively). Hens at location EI had greater egg production than hens at FW and C. Egg production for hens at tier T was also greater than for hens at tiers M and B. Egg production for hens at EI and C decreased quadratically, whereas that for hens at FW decreased linearly from tiers T to B. Cage location, but not tier level, affected egg weight. Hens at EI and FW produced heavier eggs than hens at C. Shape index, yolk color, and yolk index were independent of cage location and tier level. Hens at EI and FW produced eggs with thinner and weaker shells than hens at C. Moreover, eggshell strength increased linearly from tier T to B. Both albumen index and Haugh unit were the greatest for hens at FW, followed by EI and C. Their responses to cage location varied with tier levels. In conclusion, variation in light intensity in multitier cage systems in semiconfined laying hen houses may be a contributing factor for depressed laying performance and egg quality.     

日光温室黄瓜新品种中农21号的选育

中农21号是以优良自交系0227和00207为亲本配制而成的一代杂种。植株生长势强,早熟,丰产,耐低温弱光能力强；瓜条长棒形、顺直,瓜色深绿,瓜把短,瘤小,刺白、密,腰瓜长35 cm左右,横径约3 cm,单瓜质量200 g左右,商品瓜率高,风味品质好；高抗白粉病,抗角斑病,中抗黑星病、枯萎病、CMV、WMV,日光温室越冬栽培每667 m~2产量10 000 kg左右；适宜日光温室越冬茬和秋冬茬栽培,已在山东、河北、河南、辽宁等省推广1 333 hm~2。     

二月兰耐阴性的研究

用黑色遮阴网设置四种光强(全日照、全日照的33%、全日照的11%、全日照的3%),研究二月兰的耐阴性,结果表明:二月兰具有较典型阴生植物结构特点,其叶绿素含量随着光强降低而增加,叶绿素a/b则降低。二月兰对光照适应能力较强,全日照的3%下仍具有很高的观赏价值。但最适光照为全日照的33%。     

应用轻型飞机防治马尾松毛虫的试验Ⅰ．喷施四种药剂防治的效果

马尾松毛虫Dendrolimus punctatus Walker是马尾松林重要害虫。林间应用超轻型飞机大面积喷洒溴氰菊酯超低容量喷雾、白僵菌纯孢子油剂、菌药混合液超低容量喷雾以及喷洒白僵菌菌粉,其防治效果分别为96．5%、85．7%、95．6%、86．5%。上述的防治措施可作为单独的措施或综合防控中的配套措施使用,同时应用飞机防治解决了树高、林密、水源缺乏、劳力工资昂贵等条件给防治工作带来的困难,是一种适应林区林业生产整体水平而又快速、高效、经济的防治手段。     

UV-B radiation increases paraquat tolerance of two broad-leaved and two grass weeds in relation to changes in herbicide absorption and photosynthesis

Depletion of the protective ozone layer in the atmosphere leads to increasing UV‐B radiation on the earth's surface with potential effects on the response of plants to different stresses. Abutilon theophrasti, Amaranthus retroflexus, Digitaria sanguinalis and Chloris virgata are common weeds encountered in most arable fields in China. The effectiveness of herbicides used in controlling these weeds needs to be evaluated with increased UV‐B radiation. Seedlings of these four weeds were therefore grown under ambient and elevated UV‐B radiation to compare the efficacy of paraquat, a commonly used contact herbicide. Irrespective of species sensitivity to radiation, the elevated UV‐B radiation decreased the effectiveness of paraquat. Net photosynthesis rate was adversely affected except for D. sanguinalis, while the chlorophyll content was significantly reduced in A. retroflexus and C. virgata. UV‐B treatment increased the leaf surface wax and decreased the absorption of ¹⁴C‐paraquat in A. theophrasti, D. sanguinalis and C. virgata. These results suggest that the response of weeds to paraquat or the use of the herbicide may be affected by increased UV‐B radiation, to the extent that larger doses may be required to achieve desired effects. This may have damaging consequences for the environment.     

Does soil nitrogen affect early competitive traits of annual weeds in comparison with maize?

Soil nitrogen (N) is considered an important driver of crop‐weed interactions, yet the mechanisms involved have been only partially explored, especially with respect to early‐season growth, when competitive hierarchies are formed. This study characterises the effects of different N levels on biomass accumulation and plant morphology for maize (Zea mays), and four important weed species (Amaranthus retroflexus, Abutilon theophrasti, Setaria faberi, and Chenopodium album). Under glasshouse conditions, plants were grown in separate pots and irrigated with nutrient solution at four N concentrations (0.2, 0.5, 2, 5 μm L⁻¹) until 57 days after emergence. Except for S. faberi, which was unresponsive to N, the relative biomass growth rates (RGR) of maize and the broad‐leaved weeds were positively and similarly affected by increasing nitrogen. At all N levels, maize had a height advantage by virtue of its larger seed size, which conferred early growth benefits independent of RGR. At low N, biomass growth was instrumental to S. faberi’s improved competitive position, whereas height development per unit biomass improved the competitive position of A. theophrasti, C. album and A. retroflexus. The approach presented could be applied to other crop‐weed systems to evaluate environmental impacts on competitive outcomes.     

鹅掌楸容器育苗轻基质配方研究

选用泥炭、稻壳、森林表土、黄心土混合配制成5种轻基质配方,开展鹅掌楸容器育苗技术研究。结果表明:不同轻基质配方之间的重量,容器苗苗高、地径、根系、单株干物质重量达到极显著差异;4号轻基质配方(泥炭50%+稻壳20%+森林表土20%+黄心土10%)育苗效果最好,苗木粗壮、根系发达、基质重量较轻;1号轻基质配方(泥炭20%+稻壳50%+森林表土20%+黄心土10%)效果最差。研究认为:在鹅掌楸容器育苗生产上宜选用4号轻基质配方。     

光照对棉花胚珠纤维离体发育的影响

分析了光照和黑暗条件下，对培养棉花胚珠纤维离体发育的影响。表明在其它条件相同时，纤维离体发育在基因型之间有很大的差异，光照对纤维的发育起了明显的抑制作用。无论受精胚珠还是未受精胚珠，在光照条件下其胚珠成活率、纤维诱导率、纤维生长量、胚珠鲜重均低于在黑暗条件。尤其是未受精胚珠，在光照条件下珂３１２ 与ＴＭ－ １虽能诱导产生纤维，但纤维诱导率很低，仅有８．１９％ 和４．９６％ ，纤维生长量为０．１６３７和０．１１３２，而浙５０６、罗甸铁子和徐州１４２无絮突变体均不能产生纤维。本试验表明：只有在黑暗条件下才适合纤维离体发育。