Exogenous Nitric Oxide Involved in Subcellular Distribution and Chemical Forms of Cu~（2＋） Under Copper Stress in Tomato Seedlings

Nitric oxide（NO）,a bioactive signaling molecule,serves as an antioxidant and anti-stress agent under abiotic stress.A hydroponics experiment was conducted to investigate the effects of sodium nitroprusside（SNP）,a NO donor,on tomato seedlings exposed to 50 μmol L-1CuCl 2.The results show that copper is primarily stored in the soluble cell sap fraction in the roots,especially after treatment with Cu＋SNP treatment,which accounted for 66.2% of the total copper content.The copper concentration gradually decreased from the roots to the leaves.In the leaves,exogenous NO induces the storage of excess copper in the cell walls.Copper stress decreases the proportion of copper integrated with pectates and proteins,but exogenous NO remarkably reverses this trend.The alleviating effect of NO is blocked by hemoglobin.Thus,exogenous NO is likely involved in the regulation of the subcellular copper concentrations and its chemical forms under copper stress.Although exogenous NO inhibited the absorption and transport of excess copper to some extent,the copper accumulation in tomato seedlings significantly increased under copper stress.The use of exogenous NO to enhance copper tolerance in some plants is a promising method for copper remediation.     

秦岭林区雷击火的发生和预防

结合实践,分析了秦岭林区雷击火发生、蔓延的条件和趋势,提出了预防和扑救的技术与方法.     

4种豆科牧草种子对绵羊消化道作用的反应

以4种野生豆科牧草苦豆子(Sophora alopecuroide)、线叶野豌豆(Vicia tenuifolia)、光果甘草(Glycyrrhizae glabrae)、罗马苜蓿(Medicago romanica)种子为研究对象,测定各种子在绵羊消化道作用前后的形态、生物学特性变化以及在绵羊消化道内的运移和排放规律。结果表明,4种种子排放过程均符合高斯模型,且苦豆子、线叶野豌豆、光果甘草排放峰值均出现在饲喂后24~36h,罗马苜蓿种子排放峰值则出现在18~30h。苦豆子、线叶野豌豆、光果甘草和罗马苜蓿种子总回收率分别为14.513%、4.463%、10.811%、4.608%,在绵羊消化道内的平均滞留时间分别为36.3、29.6、31.2和31.9h。除苦豆子外,其它3种植物种子在经过消化道后单粒重显著降低(P0.05),4种种子消化后的形状指数较经过消化道前显著减小。经过绵羊消化道后种皮颜色加深,纹理模糊,出现褶皱及明显侵蚀斑。与经过消化道前比较,4种植物种子经过消化道后的发芽率均有显著提高,其中发芽率提高效果最明显的时间段在消化24~48h后。消化道作用可有效打破苦豆子、线叶野豌豆、光果甘草、罗马苜蓿种子的硬实,4种植物种子均可通过绵羊消化道传播。     

梭梭属不同种源种子品质初评

梭梭属(Haloxylon Bge.)的两个种,主要分布在新疆准噶尔盆地,是优良防风固沙和薪炭树种,被列为国家重点保护植物。国内对两种梭梭的利用和研究,从50年代末就已经开始进行,至今已做了大量的工作,但对其种子特性的研究报道甚少。为了更深入了解梭梭两种植物的种子特性,我们于1987年选用吐鲁番沙漠研究站引种成功的两种梭梭(H.ammodendron、H.Persicum)的种子同原分布区莫索湾沙漠研究站采的种子,对其品质进行了对比试验分析,为梭梭属引种工作中的种源选择及种子保存提供依据。现将结果报道如下。     

模拟降水量对毛乌素沙柳幼苗蒸发蒸腾的潜在影响

选择毛乌素沙地优势灌木沙柳为对象,研究157.5、315、472.5和630 mm 4种人控年降水量,对沙柳幼苗蒸发蒸腾作用的影响.结果表明,沙柳蒸发蒸腾量随着降水量的增加而逐渐增大,从157.5 mm增至630 mm,日蒸散量分别为1.63、3.11、4.37和5.66 mm,其中沙地蒸发量占主要部分.少量降水时,幼苗蒸腾速率明显呈现"午睡”现象,而充足降水则能消除"午睡”现象.相关分析结果表明,环境因子,如沙地含水率、太阳有效辐射、大气温度和饱和水汽压差,以及植物因素,如叶面积指数、叶片温度和气孔导度等,均能显著影响沙柳的蒸腾作用.     

灰飞虱不同虫量与水稻条纹叶枯病发病的相关性研究

通过田间水稻1代灰飞虱成虫高峰期罩笼接虫传毒试验,结果表明,带毒灰飞虱属持久性传毒型介体昆虫,并随虫量的增加,水稻条纹叶枯病发病呈上升趋势,以400～600头/m2时发病较为明显。浸种试验显示,用25%咪鲜胺EC(使百克) 10%二硫氰甲烷EC(浸种灵) 12.5%吡虫啉SL浸稻种有控虫防病效果。     

用免疫分离法检测稻种上的白叶枯病菌

 免疫分离法是一种新型的、高度灵敏和准确的种菌带菌检验法,它结合了利用专化性抗血清的选择法吸附作用和活细菌可形成菌落的两方面的优点,从种苗材料中分离到目标细菌,用纯菌测试它的回收率为50~70%,最低检出率为50~100个菌/ml,人工喷接种和病田收获的病种带菌率达87%,平均每粒自然病种上的细菌数在100个左右。免疫分离所得菌落经致病性测定,大多数为白叶枯菌。     

棉花幼苗侧根发生的化学诱导研究 Ⅲ.Ca~(2+)对棉花幼苗侧根发生和根系IAA含量的影响研究初报

清水或 DPC 200ppm 溶液浸种培养的棉花幼苗在移栽时用含 Ca~(2+)30ppm 的 CaCl_2溶液漫根2小时处理,栽后8天观察发现,两种浸种方式,Ca~(2+)均能促进棉苗侧根的发生,并提高根系 IAA 含量。但 Ca~(2+)处理 DPC 降低根对棉酚含量的效应无明显影响。上述试验结果显示出 Ca~(2+)与诱导棉苗侧根发生和调节根系 IAA 水平之间存在着某些内在的联系,这可能涉及到 Ca~(2+)·CaM系统(胞内第二信使系统)与植物激素效应之间的关系。     

Pod, Seed and Germination Characteristics of Selected Cardamom (Elettaria cardamomum (Linn.) Maton) Varieties

Cardamom is a popular and important tropical spice, traditionally propagated by rhizomes, although seeds which are considered recalcitrant are being increasingly used in planting programmes. Thus a study evaluated the effects of pod maturity on pod, seed and germination characteristics of three popular varieties, along with the influence of drying on seed germinability.
Green and yellow mature pods and seeds of all varieties had a high moisture content. The variety Mysore, which is considered high yielding, had larger pods with a greater number of heavy seeds. Although the pods of Vazukka were heavier than the Malabar variety due to greater seed weights, seed numbers were similar in both varieties. Seed moisture contents declined with increasing pod maturity, while seed weights and germinability increased.
The germinability of Mysore seeds were greater, while that of the other varieties were lower. The larger seeds of all varieties germinated 15–20 days earlier, giving rise to a greater percentage (20–25 %) of seedlings. Desiccation at 20 °C maintained germinability for a longer period (5–9 days) in all varieties, than when dried at 30 °C. The seeds of the variety Mysore was more tolerant to desiccation than other varieties. The study illustrated the importance of selecting large seeds from yellow mature pods of cardamom for propagation and the value of establishment immediately after harvest to obtain a greater number of seedlings.     

江泽民法治思想初探

江泽民从建设社会主义市场经济和民主政治,维护国家长治久安的客观需要出发,继承与发展邓小平法治思想,提出了"依法治国、建设社会主义法治国家"的治国方略;江泽民法治思想的核心和最高成就是依法治国,建设社会主义法治国家;其法治思想的具体内容包括加强立法、依法行政、司法改革、普法教育、发展法律服务业.