全运会竞赛制度变迁方向探索

通过对历届全运会竞赛规程的竞赛举办时间、地点、项目设置、运动员参赛资格、奖励和计分办法等条款进行分析，认 为全运会经过不断演变，已形成奥运会次年办赛、项目设置以夏季奥运项目为主并兼顾民族传统体育项目和全国各地竞争办赛 的特点；运动员交流办法已趋于完善，奥运会成绩计分、两次计分、球类加倍计分等计分办法逐渐演变成我国全会运会特殊的 计分制度。建议将已经有过办赛经验的省、市援助中、西部欠发达地区申办全运会，制定办赛奖励制度；对交流运动员交流年 限延长至一个全运会周期，重视“新型联合培养”；根据全运会发展需要，进一步加大计分奖励力度。     

森林保险制度体系重塑与运行机制优化

森林保险市场存在有效需求不足、创新供给乏力、地方政府参与积极性低的供需双冷局面,财政补贴政策低效运行。其根本原因是缺乏独立的适应其特殊性的森林保险制度体系作为保障,致使现有运行机制不协同,产品体系不科学,补贴机制不合理,导致市场发展缓慢。为此,通过林业风险与林业生产的属性特征分析,确定我国森林保险属性定位应是有政府支持的商业性森林保险,在此基础上围绕运行模式、产品体系、财政补贴体系建立相应的运行机制,从而构建一套独立综合的森林保险制度体系与运行机制,以改善供需双冷市场僵局,保障财政补贴政策有效发挥,推动森林保险健康持续发展。     

Dopamine Antagonist Affects Luteal Function But Not Cyclicity During the Autumn Transition

The autumn transition is characterized by a number of anomalies affecting the luteal phase of the estrous cycle, such as declining progesterone secretion from the corpus luteum and increased rate of failure of luteolysis. Prolactin also decreases. We theorized that these events may be linked, possibly through dopamine. We administered domperidone, a specific dopamine D2 receptor antagonist from September 12 to anestrus or January 15, either daily (n = 8), or once per day for the first 7 days of each month (n = 7). Controls (n = 7) received no treatment. Mean progesterone and prolactin concentrations were compared with summer cycles. Time to entry into anestrus and incidence of spontaneously prolonged luteal activity was compared between groups during the autumn transition. Prolactin declined from June through October equally in all groups. There was no difference between groups in time to anestrus. Progesterone decline was prevented (daily treatment) or delayed for a prolonged period (weekly group) in autumn. The incidence of spontaneously prolonged corpus luteum activity was reduced to summer levels in both domperidone groups compared with the control. We concluded that autumn prolactin decline was not driven through dopaminergic D2 receptor inhibition of pituitary lactotrophs. Sustained progesterone synthesis through the autumn may be the result of action of the D2 receptor antagonist with dopamine receptors on the corpus luteum. Autumnal luteolytic function appears to have a dopamine-influenced component. There does not appear to be a causative relationship between autumnal progesterone and prolactin secretion.     

马克思的公平理论与和谐社会的建构

马克思在运用唯物史观深入剖析和批判资产阶级和小资产阶级社会主义者的公平观的过程中,阐述了马克思主义关于公平的理论,指出公平是一个历史的、社会的、阶级的范畴。马克思的公平理论,是人类历史上对于公平问题探讨的集大成者,是阶级性、革命性和科学性的统一,成为指导无产阶级和广大人民群众争取自身解放的有力武器。维护社会公平,是建设和谐社会不可或缺的重要组成部分。维护社会公平,首先要大力发展经济;科学发展观是推动我国经济社会发展的根本指针和维护社会公平所应遵循的基本原则;我们必须建立健全保障社会公平的制度,制度能营造、保护和巩固社会公平的成果。     

Calreticulin promotes migration and invasion of nasopharyngeal carcinoma cells by inducing cell EMT

AIM:To observe the expression of calreticulin (CRT) in nasopharyngeal carcinoma tissues, analyze the significance of clinical pathology and the influence on epithelial-mesencymal transition (EMT) of CNE2 cells. METHODS:The expression of calreticulin was detected by immunohistochemistry in 52 nasopharyngeal carcinoma and 57 nasopharyngeal benign tissues, and the significance of clinical pathology was evaluated. The calreticulin gene-specific small interfering RNA was constructed, and then was transfected into the NPC cell line CNE2 using the cationic liposome method. The effect of CRT on the morphological changes of the CNE2 cells was observed under light microscope. The effect of CRT on the cell migration and invasion abilities of the CNE2 cells was detected by Transwell migration and invasion assays. The expression of EMT-related proteins E-cadherin, vimentin, transforming growth factor (TGF)-β and matrix metalloproteinase (MMP)-9 in the CNE2 cells was determined by Western blot. RESULTS:The positive expression rate of CRT in the benign lesion tissues was 19.29% (11/57), which was significantly increased in the nasopharyngeal carcinoma tissues as 82.69% (43/52). The expression rate of CRT was positively correlated with the stage of nasopharyngeal carcinoma and lymph node metastasis (P<0.05). Knockdown of CRT expression made the CNE2 cells showing a spindle shape to a flat, cobblestone-like epithelial state change, arranged more compact, and the migration and invasion abilities were significantly decreased (P<0.05). Knockdown of CRT expression resulted in significant increase in the protein expression of E-cadhe-rin, and the decreases in the protein expression of vimentin, TGF-β and MMP-9 in the CNE2 cells (P<0.05). CONCLUSION:Calreticulin expression in nasopharyngeal carcinoma is significantly higher and positively correlated with nasopharyngeal carcinoma stage and lymph node metastasis. Calreticulin promotes cell migration and invasion of nasopharyngeal carcinoma CNE2 cells by inducing EMT.     

Juglone inhibits epithelial-mesenchymal transition of prostate cancer cells by regulating Wnt/β-catenin/Snail signaling pathway

AIM: To investigate the mechanism of juglone on epithelial-mesenchymal transition in prostate cancer cells. METHODS: Human prostate cancer LNCaP cells were divided into control group (without juglone), 12.5 μmol/L juglone group and 25 μmol/L juglone group. LNCaP cells in the latter 2 groups were treated with juglone for 24 h. The invasion ability of the LNCaP cells was detected by Transwell assay. The protein expression of E-cadherin, vimentin, Snail and β-catenin was determined by Western blot. The LNCaP cells were treated with LiCl and juglone in combination for 24 h, and the protein expression of Snail and E-cadherin was detected by Western blot.RESULTS: The results of Trans-well invasion assay showed that the invasion ability in juglone groups was significantly decreased (P<0.01). The protein expression of E-cadherin in the LNCaP cells treated with juglone was increased, and the expression levels of vimentin and β-catenin were reduced (P<0.01). Treatment with LiCl significantly attenuated the inhibitory effect of juglone on Snail expression and subsequent down-regulation of E-cadherin expression. CONCLUSION: Juglone inhibits the epithelial-mesenchymal transition by inhibiting the Wnt/β-catenin/Snail signaling pathway in the LNCaP cells.     

Over-expression of SCUBE2 suppresses epithelial-mesenchymal transition through Wnt/β-catenin signaling pathway in colorectal cancer cells

AIM: To study the effect of SCUBE2 on epithelial-mesenchymal transition (EMT) in colorectal cancer cells and its mechanism. METHODS: The expression of SCUBE2 in human colorectal cancer cell line HCT116 and normal colonic cell line FHC was detected by real-time PCR and Western blot. HCT116 cells were transfected with GV144-SCUBE2 to over-express SCUBE2, and then the cell viability, migration, and apoptosis were determined by MTT assay, Transwell assay and flow cytometry, respectively. The expression of EMT markers (E-cadherin, vimentin, and Snail), β-catenin, c-Myc and cyclin D1 in the HCT116 cells was analyzed by real-time PCR or Western blot after transfection with GV144-SCUBE2 for 6 h, followed by the stimulation of 10 μg/L recombinant TGF-β1 protein for 48 h. Additionally, the EMT process of HCT116 cells, which were stimulated by TGF-β1, over-expressed SCUBE2, and treated with Wnt/β-catenin pathway activator lithium chloride (LiCl) or inhibitor XAV93920, was analyzed by Western blot. RESULTS: Compared with FHC cells, the expression of SCUBE2 in the HCT116 cells was significantly decreased. The viability and migration ability of the HCT116 cells were suppressed by SCUBE2 over-expression, but the apoptosis was not markedly changed. Elevated expression of SCUBE2 increased E-cadherin expression, and decreased the expression of vimentin, Snail, β-catenin, c-Myc and cyclin D1 induced by TGF-β1. Treatment with LiCl blocked but treatment with XAV93920 enhanced the effects of SCUBE2 on EMT. CONCLUSION: Over-expression of SCUBE2 may inhibit the cell growth and migration, and suppress EMT through Wnt/β-catenin signaling pathway.     

全膜双垄沟起垄覆膜机横向土带堆放机构的设计与试验

为增强全膜双垄沟起垄覆膜机的覆膜、覆土质量,聚集降水,减少水土流失和地表径流,提出偏置轴承式、凸轮顶杆式、地轮拨齿式3种横向土带堆放机构,运用SolidWorks Motion对3种机构进行运动仿真与机构分析,优选出工作时间短、急回特性明显的横向土带堆放机构,并进行田间验证试验。仿真结果表明:在相同仿真参数情况下,地轮拨齿式横向土带堆放机构完成1次抖土压膜工作所需时间仅为0.29 s,抖土板位移为530.45 mm,倾角和张角分别为29.00°、21.39°,最大电动机消耗功率最小。田间验证试验表明:作业后横向土带宽度合格率为96.5%,间距合格率为95.3%,厚度合格率为95.5%。相比较偏置轴承式和凸轮顶杆式,地轮拨齿式机构放土质量均匀,急回效果好,不易粘土,能耗最少,覆土覆膜效果较好,符合全膜双垄沟作业要求,适宜于全膜双垄沟覆膜种床横向土带堆放。     

玻璃化转变温度对层压板性能的影响

探求层压板的玻璃化转变温度（ｔｇ）对层压板性能的影响,对研制开发层压板新产品和选择适用层压板制作印刷电路板有重要的理论意义和实际意义。对不同ｔｇ的层压板的耐热性、抗弯强度和热膨胀性等８项性能进行了比较。结果表明层压板的ｔｇ升高,层压板的机械和电气性能明显提高。