线粒体与细胞凋亡

细胞凋亡是一种由基因控制的细胞自杀性死亡过程,是机体维持自身稳定的一种基本生理机制。机体通过细胞凋亡清除损伤、衰老与突变的细胞,维持生理平衡。通过对细胞凋亡的研究发现,线粒体在调节细胞凋亡中发挥着关键作用。尤其是被称为细胞凋亡主开关的线粒体通透性改变孔(MitochondrionPermeabilitytransitionPore,MPTP),它是细胞色素C、Smac、AIF等凋亡诱导因子的主要来源。本文以MPTP的开放、细胞色素C、AIF在细胞凋亡中的作用以及Bcl-2家族对细胞凋亡的调控做一综述,以便广大读者能系统地了解线粒体在细胞凋亡中的作用。     

双子叶子叶出土植物根-茎转变区的特征和分类

利用偏光显微镜镜检等手段,研究了14科22个属、种的双子叶植物的根-茎转变区,提出较全面的有关概念并发现数种异于一般的转变方式,根据该区的存在部位和长短以及结构特点,提出几套新的分类法:1.根型、根-下胚轴和下胚轴型;2.剧变型及缓变型;3.正常型和异常型。     

制度变迁与制度绩效：来自中国农地的分析

制度经济学的理论可以用来分析中国农地制度变迁及其绩效。寻求最佳的绩效一直是制度变迁的终极目标。50多年来中国农地制度变迁一直在实践着一种效率更高的制度对另一种制度的替代。中国未来农地制度的变迁必须遵循土地产权制度安排的稳定性和资源最佳配置效应原则、激励机制健全原则、农地制度安排的渐进式原则和农村社会保障体系健全原则。     

河北坝上地区可持续发展的创新思路

坝上地区可持续发展应充分考虑我国经济发展的现状和其所处的大北京地区的重要区位,以全球变化为背景,以持续发展理论为先导,大胆更新观念,形成一套创新制度:建设“人控环境”,实施“大范围搞生态重建,小区域搞集约生产”制度;城市化与产业化相结合,采取“鼓励农业人口转化为非农业人口,以产业化带动城市化”的积极的城市化制度;沙漠化防治与治贫治愚相结合,采取“治愚-治沙-产权-受益”制度;确立产权制度和相应利益分配机制,对口支援与生态效益转移收费制度;生物技术与工程技术相结合,制定鼓励科技创新制度。     

Modelling land cover transitions: A solution to the problem of spatial dependence in data

Raster-based spatial land cover transition models (LCTMs) are widely used in landscape ecology. However, many LCTMs do not account for spatial dependence of the input data, which may artificially fragment the output spatial configuration. We demonstrate the consequences of ignoring spatial dependence, thus assigning probabilities randomly in space, using a simple LCTM. We ran the model from four different initial conditions with distinct spatial configurations and results indicated that, after 20 simulation steps, all of them converged towards the spatial configuration of the random data set. From an ecological perspective this is a serious problem because ecological data often exhibit distinct spatial configuration related to ecological processes. As a solution, we propose an approach (region approach) that accounts for spatial dependence of LCTM input data. Underlying spatial dependence was used to apply spatial bias to probability assignment within the model. As a case study we applied a region approach to a Vegetation Transition Model (VTM); a semi-Markovian model that simulates forest succession. The VTM was applied to approximately 500,000 ha of boreal forest in Ontario, at 1 ha pixel resolution. When the stochastic transition algorithms were applied without accounting for spatial dependence, spatial configuration of the output data became progressively more fragmented. When the VTM was applied using the region approach to account for spatial dependence output fragmentation was reduced. Accounting for spatial dependence in transition models will create more reliable output for analyzing spatial patterns and relating those patterns to ecological processes.This revised version was published online in May 2005 with corrections to the Cover Date.     

金安桥水电站初次蓄水对导流洞稳定性影响的计算分析

金安桥水电站下闸蓄水后，陡升的库水位将对导流洞结构稳定造成不利影响。为保障堵头封堵前的导流洞结构稳定性，运用三维弹塑性损伤有限元，在模拟进水口边坡及导流洞开挖支护的基础上，计算汛前下闸水位时的岩体渗流场，对导流洞围岩和衬砌在岩体渗流作用下的结构稳定性进行复核分析。分析结果表明，导流洞的围岩稳定性在蓄水后仍有保障，衬砌结构受力较小，现有环向配筋满足结构内力要求，但在进水口变截面和导流洞变截面部位沿水流方向的拉应力较大，使得衬砌出现开裂。进一步分析表明，上述区域的开裂范围有限，对整体衬砌结构影响较小。     

Increasing selenium supply during the close-up dry period improves nutrient metabolism and attenuates inflammatory response after calving in dairy cows

The objective of this study was to investigate whether feeding selenium (Se)-replete cows a Se-yeast supplement in late pregnancy affects nutrient metabolism and inflammatory response during the periparturient period. Twenty cows were randomly assigned to two groups with 10 cows each. Cows in one group received Se-yeast at 0.3 mg Se/kg DM during the last 4 weeks before calving in addition to fed a TMR containing supplemented sodium selenite at 0.3 mg Se/kg DM (Se-yeast), while cows in another group were only fed a TMR containing supplemented sodium selenite at 0.3 mg Se/kg DM (Control). Blood samples were collected and analyzed for nonesterified fatty acids (NEFA), β-hydroxybutyrate (BHBA), glucose, insulin, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, serum amyloid A (SAA), haptoglobin (Hp), and albumin. In control cows, plasma NEFA, IL-1β, IL-6, SAA, and Hp levels increased after calving, but glucose, insulin, and albumin levels decreased after parturition. Se-yeast supplemental cows had lower postpartum concentrations of NEFA, TNF-α, IL-1β, IL-6, SAA, and Hp, and higher postpartum levels of glucose, insulin, and albumin compared with control cows. The results indicate that feeding Se-replete cows a Se-yeast supplement in late pregnancy improves nutrient metabolism and attenuates the inflammatory response after calving.     

农业专业镇中小微企业服务平台发展战略初探——以广东省为例

农业专业镇是广东省农村经济发展的重要推动力。如今农业专业镇的发展面临着结构调整、产业升级和增长方式转变的巨大挑战，服务平台发展的战略选择对农业专业镇发展至关重要。专业镇的中小微企业服务平台建设发展呈现出以创新为核心、产学研紧密结合的趋势：加快中小微企业服务平台发展，在战略上必须以政府为主导，市场化配合运作，将载体建设与功能培育协同运作，将产学研合作与联盟服务协同运作，将品牌建设与重点扶持紧密结合，形成协同运作体系网。     

基于动态平衡视角下现代足球阵型演变的沿革与变迁

拟在动态平衡的视角下对现代足球阵型的演变历程进行梳理与整合，希望对阵型之间的关联及其对足球运动发展的影响提供见解，对阵型演变的不同阶段进行探讨，探寻足球攻守平衡与不同阵型的嬗变关系，同时也为推动未来足球阵型演变对战术打法的影响研究提供参考依据。采用文献资料、系统分析、专家访谈和逻辑分析等方法，对现有的足球阵型发展的概况进行分析之后，认为现代足球阵型的演变历程不断交替出现攻守的平衡与不平衡，正是交替出现的状况促进了阵型的改变，进而影响了阵型的发展。当阵型的发展过程中出现攻守平衡时，就有其它后来者对该阵型进行学习、模仿、改进、提高。当阵型的发展过程中出现攻守不平衡，攻强于守或者守强于攻时，就会促进阵型的演变向攻守平衡的方向发展，因此就会出现阵型的发展处于不平衡-平衡-不平衡-再到平衡的态势，从而形成比赛阵型发展本身的稳定与不稳定状态交替出现。研究认为，世界足球阵型演变的过程是一种动态的、不断发展的、维持某种平衡与稳定的、平衡和不平衡交替运动的情况，即动态平衡的状态。提出将现代足球阵型演变的过程分为不同的阶段，每一个阶段内，都分别出现攻守平衡与攻守不平衡的状态，而这种平衡与不平衡的存在与区别、斗争与转化正是足球阵型演变与发展的规律。     

Low potassium diets with different levels of calcium in comparison with different anionic diets fed to prepartum dairy cows: Effects on sorting behaviour,total tract digestibility,energy metabolism,oxidative status and hormonal response

This study investigated the effects of low potassium diets with different levels of Ca compared to two diets low in dietary cation–anion difference (DCAD) fed prepartum as a strategy to prevent hypocalcemia on sorting behaviour, total tract digestibility, oxidative status and energy and protein metabolism of transition cows. Forty-eight pregnant dairy cows were assigned to 4 treatment groups: Low Ca, low K (LCLK), High Ca, low K (HCLK), Supplementation with anionic mineral mixture (AMS) supplementation with SoyChlor (CAS). After parturition, all animals were fed a standard postpartum diet. Data were collected until 21 DIM. Prepartum urinary pH was significantly reduced by the low DCAD diets, while postpartum Ca homeostasis was affected by the HCLK ration. Feeding AMS induced sorting against particles <1.18 mm in favour of particles >19 mm prepartum. In contrast, cows fed CAS showed an increase in selective consumption of fine particles and sorted against longer particles similar to the HCLK and LCLK groups. Postpartum sorting activity was not affected by the dietary treatments. After calving, apparent digestibility of NDF was significantly reduced in the HCLK group. Prepartum, we observed effects on serum concentrations of non-esterified fatty acids were higher and insulin sensitivity was lower in the AMS group. Blood urea nitrogen (BUN) was decreased in cows fed the CAS ration. Postpartum, we found serum protein to be decreased with the low DCAD diets while BUN was decreased in the CAS group. The low DCAD rations increased prepartum serum malondialdehyde concentrations, while postpartum total antioxidant capacity was lower in the HCLK and the AMS group. From these data, we conclude that AMS decreased prepartum intake due to compromised palatability. Intermediate protein metabolism was affected by the low DCAD diets, while parameters of oxidative stress were probably affected by acid–base balance and Ca homeostasis.