睾丸支持细胞的功能、分离纯化及鉴定研究进展

支持细胞对维持精子形成过程中的微环境起决定作用,它可以通过分泌功能、细胞间连接形成的血睾屏障功能以及吞噬功能等来促进精子的形成过程,其发育异常会导致不同程度的雄性生殖缺陷。基于支持细胞在雄性动物生殖过程中的作用,体外培养高纯度支持细胞可成为研究睾丸两大核心功能-精子发生和性激素分泌功能相关调节机制重要的细胞模型。此外,体外培养睾丸支持细胞也可作为生殖毒理学等新兴热点领域的细胞模型,为评估和研究环境因素对雄性生殖的影响提供便利。因此,作者系统地归纳、总结了目前关于动物支持细胞生物功能的研究及常用的体外分离纯化、培养及鉴定方法,以期为利用动物支持细胞开展雄性生殖领域的研究提供参考。     

基于LLWin3.0控制Fischertechnik模型实例

随着计算机的普及，特别是软件技术的发展，基于电脑控制且充分展示个性化创新实验，成为当今高等学校素质教育的重要方式和发展方向。为此，详细介绍了德国慧鱼公司的专利产品“创意组合模型(fischertechnik)的应用实例。结合LLWin3．0图形化编程技术。为教育行业应用提供了有效的途径。     

高效节水灌溉管道流量计算原则初探

对于管网优化及投资分析来说,最不利情况下的水力条件不是普遍条件,不能够完全代表管网应当有的优化假定条件。通过管道控制灌溉面积来确定管道的流量,按照满足在一天内,管道控制的所有灌水器都灌溉一次的原则确定,并给出公式。让管道的流量这一重要参数,在高效节水灌溉工程优化和设计计算中变得简单并容易计算。     

水头可调薄壁堰式渠道流量智能测控系统优化试验研究

采用巴赞堰流公式计算流量的水头可调薄壁堰式渠道流量智能测控系统通过渠道流量量测与控制试验,验证了其精准的测流功能和智能控制技术的可行性。为进一步提高该测控系统运行的效率和稳定性,减少运行能耗,对矩形薄壁堰堰流公式进行水力试验研究,通过分析堰上水头和流量关系,拟合出矩形薄壁堰流量计算公式。通过向该拟合公式引入修正系数λ=0.848,将测控系统测得的堰上水头数据值代入该拟合公式求得的流量值与电磁流量计测得流量值的差值的平均值为-0.004L/s,标准差为0.119,线性相关系数R2=0.989 1。该拟合公式相对于巴赞堰流公式形式简单、参数较少、运算简便,同时也满足节水灌溉对精准测流的要求,更适用于水头可调薄壁堰式渠道流量智能测控系统,对于在灌区推广和使用该测控系统具有重要的意义。     

日光温室青花菜耗水规律及水分利用效率的研究

在膜下滴灌条件下,研究了温室秋茬青花菜不同生育阶段控制水分对耗水规律、水分利用效率以及产量的影响。结果表明,青花菜全生育期内耗水量、耗水强度均表现为苗期﹥莲座期结球期的规律;水分生产函数的敏感指数为结球期﹥苗期﹥莲座期,因此青花菜节水宜在莲座期进行,结球期应保证水分供应充足,避免减产;苗期和莲座期分别控制灌水量为常规灌水量65%的处理,产量高、水分利用效率较高,是青花菜适宜的灌溉量。     

土壤入渗传输函数输入变量分析

依据土壤水分入渗参数与其相关土壤理化参数之间的相关性,从土壤学的角度用物理的方法分析应县土壤常规土壤理化参数对土壤入渗传输函数影响程度,进一步确定其土壤入渗传输函数的输入变量。并用大田耕作土壤水分入渗参数及其土壤理化参数的实测资料,建立土壤入渗传输函数进行验证。     

The Fluorescent Characterization and Analysis of Two Brucella Attenuated Vaccine Infecting Mouse Macrophagocyte

The experiment was conducted to discuss the difference of binding time of green fluorescent protein B.melitensis M5 (GFP-M5) and B.abortus S19 (GFP-S19) infecting the mouse macrophagocyte (RAW264.7),lysosome,endoplasmic reticulum and golgi body in the initial stage and compare the binding rate of GFP-M5,GFP-S19 with organelle in different timeline,respectively,by confocal laser scanning microscope (CLSM) and flow cytometry.The result showed that GFP-M5 and GFP-S19 were successfully constructed.The intracellular survival ability of Brucella M5,Brucella S19,GFP-M5 and GFP-S19 were not obvisouly affected after infecting RAW264.7.GFP-M5 and GFP-S19 could enter the macrophagocyte in 30 mins,and in 2 h the Brucella could reach lysosome,endoplasmic reticulum and golgi body.In addition,the binding time for two attenuated vaccine did not show differences in 1,2,3 and 4 h.The content of GFP⁺ cell produced by RAW264.7 infected by GFP-M5 and GFP-S19 did not show significant differences (P>0.05).Therefore,the two strains did not have significant differences in the invasion ability in the initial stage of infecting host cell.     

Protective Effect of Sulforaphane on Reproductive Function of Male Mice with Cadmium Poisoning

The study was aimed to explore the protective effect of sulforaphane (SFN) on the reproductive function of male mice with cadmium poisoning.40 healthy clean grade male Kunming mice were randomly divided into four groups:control group (H₂O),cadmium chloride group (2.3 mg/kg CdCl₂),sulforaphane group (10 mg/kg SFN),sulforaphane + cadmium chloride group (10 mg/kg SFN+2.3 mg/kg CdCl₂),and continuous administration for 10 d,all mice were executed by dislocated cervical vertebra at 2 d after the last administration,and then the pathologic changes of testicular tissues,organ coefficient of testicle and epididymis,sperm quality and concentration of testosterone were tested.Additionally,the contents of GSH and MDA,and the activities of T-SOD in testis were also detected at the same time. Compared with the control group,pathology damages were observed in cadmium chloride group,organ coefficient of testis and epididymis,sperm quality and levels of testosterone extremely significantly decreased (P<0.01),the activities of T-SOD and GSH content were extremely significantly decreased (P<0.01),and the concentration of MDA was extremely significantly enhanced (P<0.01).Compared with the control group,the activity of T-SOD and concentration of GSH in sulforaphane group were significantly increased (P<0.05),and the concentration of MDA was not significant different between the control group and sulforaphane group (P>0.05).While compared with the cadmium chloride group,the sperm motility rate and sperm total count in sulforaphane and cadmium chloride group were extremely significantly increased (P<0.01),the organ coefficient of testicle and epididymis was increased significantly (P<0.05),the concentration of GSH and activity of T-SOD in testicular tissue were extremely significantly increased (P<0.01),and the concentration of MDA was extremely significantly decreased (P<0.01).The results indicated that sulforaphane had the protection effect on reproduction function of male mice with cadmium poisoning.