城市轨道交通上盖物业振动及噪声影响研究进展

阐述了城市轨道交通上盖物业振动和噪声影响研究进展,为城市轨道交通上盖物业前期规划建设提供参考性依据。     

Thyroid gland development in Senegalese sole (Solea senegalensis Kaup 1858) during early life stages: A histochemical and immunohistochemical approach

A key to success in the culture of marine fish species is the mass production of high quality fry, a process largely dependent on successful first feeding and normal development and growth of fish larvae. In this regard it is important to examine the structural and functional development of the endocrine system (pituitary, thyroid, interrenal glands) during early ontogeny of marine fish. Particularly, the thyroid hormones, thyroxine (T₄) and triiodothyronine (T₃), influence numerous metabolic processes, such as growth, differentiation, metamorphosis, reproduction, respiration, migratory behaviour, central nervous system activity, seasonal adaptation, etc. Therefore the aim of this study was to describe the development of the thyroid gland and the ontogeny appearance of the thyroid hormones in Solea senegalensis larvae by means of histological and immunohistochemical techniques. The first thyroid follicle was present at 4 days-post-hatch (dph) coinciding with first feeding. During metamorphosis (12–20 dph) the follicles increased in both number and size, and by 30 dph presented the same characteristics as that seen in adult fish. Tissue immunostaining of both thyroid hormones decreased during the endogenous larvae development to nearly undetectable levels at the completion of yolk-sac absorption. During larvae exogenous phase, T₃ and T₄ immunostaining was first detected by 6 dph and an increase of specific staining for both hormones was detected between 12 and 20 dph, during metamorphosis phase.     

基于人工神经网络的PV/T热电联供系统性能预测

为研究太阳能PV/T热电联供系统的性能和针对太阳能PV/T系统复杂的能量平衡方程,搭建了太阳能PV/T系统试验台,同时建立了基于改进灰狼优化的BP神经网络(back propagation neural network model based on improved grey wolf algorithm, IGWO-BP)预测模型,在晴朗天气下进行试验,并采用该模型对系统电功率以及蓄热水箱内水温进行预测。结果显示,晴朗日系统的电效率8.7%～12.2%、热效率51.7%;预测结果与BP神经网络预测模型、基于粒子群优化的BP神经网络(back propagation neural network based on particle swarm optimization, PSO-BP)预测模型和卷积神经网络（convolutional neural network, CNN）预测模型预测结果进行比较,结果显示IGWO-BP预测模型电效率预测模型的绝对百分比误差(mean absolute percentage error, MAPE)、决定系数(determination coefficient, R ² )、均方根误差(root mean square error, RMSE)、效率因子(efficient factor, EF)和Pearson相关系数(pearson related coefficient, r )分别为4.5E-05、0.99、0.24、0.99和1.00,在储热罐温度预测中,上述指标分别为8.90E-04、0.98、0.07、0.98、0.99,均优于其他预测模型,IGWO-BP神经网络预测模型具有更好的预测性能。研究结果可为太阳能PV/T热电联供系统性能预测与优化控制提供参考。     

Preparation and comparison of biological properties of recombinant carp (Cyprinus carpio) growth hormone and its Cys-123 to Ala mutant

Carp growth hormone (cGH) cDNA, in which Cys-123 was mutated to Ala, was prepared, transferred to the expression vector, expressed in Escherichia coli and the mutant was purified to homogeneity. The mutation only slightly improved yield of the monomeric fraction, indicating that Cys-123 is not involved in improper refolding. As compared to cGH, the mutant (cGH-C123A) exhibited lower binding affinity toward homologous liver receptors and lower bioactivity in a 3T3-F442A preadipocyte bioassay despite the fact that both hormones exhibited almost identical cross-reactivity with anti-cGH antibodies. These results, along with those of a structural comparison to hGH, suggest that Cys-123 is located in the hydrophobic core of the hormone, and is most likely affecting the conformation of the binding site. Dimeric forms of the hormone and its mutant were less active than their respective monomers. Homologous binding experiments using a carp liver microsomal fraction revealed a single receptor population with Kd = 0.77 nM and B_max = 241 fmol/mg microsomal protein.

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Résumé Un ADN complémentaire (cDNA) de l'hormone de croissance de carpe (cGH), dans lequel l'acide aminé Cys-123 a été muté en Ala, a été préparé, inséré dans un vecteur d'expression, et exprimé dans Escherichia coli. Le mutant a ensuite été purifié jusqu'à homogénéité. La mutation améliore seulement faiblement la production de la fraction monomérique, indiquant que le Cys-123 n'est pas impliqué dans un repliement erroné. Comparé à la cGH, sa forme mutée (cGH-C123A) montre une plus faible affinité de liaison vis à vis de récepteurs hépatiques homologues, et une plus faible activité biologique dans un test réalisé sur des préadipocytes 3T3-F442A; cela en dépit du fait que les deux hormones présentent des réactions croisées presque identiques avec un anticorps anti-cGH polyclonale. Ces résultats, associés à une comparaison à la structure de l'hGH, suggèrent que le Cys-123 est localisé dans la partie hydrophobique de l'hormone, et affecte, le plus vraisemblablement, la conformation du site de liaison. Les formes dimériques de l'hormone et de sa forme mutée sont moins actives que leurs monomères respectifs. Les études de liaison homologue, réalisées avec des fractions microsomales de foie, révèlent une population unique de récepteurs de Kd = 0,77 nM et de B_max = 241 fmol/mg de proteine microsomale.

     

河南枸骨四新变种

描述了河南地区枸骨4新变种,即:①紫枝枸骨(Ilex cornuta Lindl.et Paxt.var.purpureoramula T.B.Zhao et J.Y.Chen,var.nov.)、②紫序枸骨(Ilex cornuta Lindl.et Paxt.var.trispinoso-duris T.B.Zhao,var.nov.)、③剌齿枸骨(Ilex cornuta Lindl.et Paxt.var.spina T.B.Zhao,var.nov.)和④多刺枸骨(Ilex cornuta Lindl.et Paxt.var.ultraspina J.Y.Chen et T.B.Zhao.Var.nov.)的形态特征和分布情况,并与原变种进行了比较。     

T型板刀式秸秆还田机刀辊设计

采用T型板式刀具和支撑切削原理进行秸秆粉碎还田,以双螺旋线形式合理布置T型刀具并设计还田机刀辊。该还田机刀辊转速低,功耗小,粉碎秸秆,破除根茬,作业质量高。     

细菌Ⅲ型分泌系统装配的研究进展

细菌Ⅲ型分泌系统的发现是病原菌致病机理的重大发现。病原菌为了生存和进入真核宿主细胞,经过长期进化逐渐形成了入侵宿主细胞的特异性机制,其中最显著的机制是细菌Ⅲ型分泌系统(T3SS)。T3SS可以将病原菌效应蛋白直接注入宿主细胞中。最初T3SS只是在少数的致病菌中发现,后来在人类、动物甚至植物的共生菌或益生菌中都有发现。近几年在T3SS的结构、装配以及致病机理的研究上取得巨大的进展。研究T3SS的装配不仅有助于探索病原菌的致病机制,还对研究细胞器装配和蛋白分泌有很大的帮助。