泡桐AFLP反应体系的建立及引物筛选

以豫杂一号泡桐为材料,通过对影响AFLP技术体系的各主要因素的研究,建立了适于泡桐AFLP分析的技术体系.结果表明最佳酶切体系(20μL)为500 ng模板DNA,3 U的Pst 1和Mse I,在37℃下双酶切3 h;20μL最佳连接体系中为酶切产物15 μL,0.25 μmol·L-1Pst I接头,2.5 μmol·L-1 Mse I接头,1 μL 10×T4 Buff-er,2 U T4连接酶,22℃连接18 h;20 μL最佳预扩反应体系中5 μL稀释10倍的连接产物,100 μmol·L-1dNTP,2 U Taq酶,250 μmol.L-1 Pst I和Mse I引物(P+AGT/M+AGT),2 μL 10×PCR Buffer.20 μL最佳选择性扩增反应体系中5 μL稀释20倍预扩增产物,100 μmol·L-1dNTP,2 U Taq酶,350 μmol·L-1 Pst I和Mse I引物(P+AGT/M+AGT),2 μL 10×PCR Buffer.最后,筛选出了97对适宜于泡桐AFLP分析的引物.     

Pb、Zn复合胁迫对台湾泡桐生长及生理指标的影响

[目的]研究重金属胁迫对台湾泡桐生长及生理指标的影响。[方法]通过液体培养研究铅(Pb)、锌(Zn)复合胁迫对台湾泡桐生长及生理指标的影响。[结果]Pb、Zn复合胁迫下台湾泡桐根、茎长度,鲜重,耐性指数随处理浓度升高而下降;不同浓度的Pb、Zn复合胁迫下,植物光合色素含量呈下降趋势,叶绿素a下降趋势显著,在100 mol/L Pb和200 mol/L Zn复合胁迫下最低,较对照(CK)下降了60%;台湾泡桐丙二醛含量在根、茎、叶中的大小为茎叶根,呈先降后升趋势;地上部分和地下部分脯氨酸(Pro)含量在100 mol/L Pb胁迫下最高,分别较CK增加24%、1.2倍,随之下降趋势显著;根部谷胱甘肽含量变化较显著;茎、叶抗坏血酸含量在Pb、Zn复合胁迫下较CK呈上升趋势,根部防御活性氧毒害的能力减弱。[结论]Pb、Zn复合胁迫浓度的增加加重了其对台湾泡桐的伤害,严重影响了植物的生长。     

Stand basal area as an index of tree competitiveness in timber intercropping

To derive optimal benefits from intercropping timber, farmers should make important initial decisions on tree species and planting density with a good understanding of their tradeoffs. Complex and data-intensive models used by researchers should be supplemented with simpler models based on easily measured parameters and easily understood competition functions. In experiments in the Philippine uplands, growth parameters of three popular farm-forestry species (Eucalyptus deglupta, E. torelliana, and Paraserianthes falcataria) were measured, along with intercropped and non-intercropped yields of maize and vegetables. The commonly used forestry parameter of stand basal area had a significant negative correlation with intercrop yields (as a percentage of non-intercropped yields). The slope of the regression line differed between species; in this study, percent yield loss per unit stem basal area growth was in the order E. deglupta > E. torelliana > P. falcataria. The relationship between stand basal area and intercrop-yield decline was tested on an independent data set from China. Intercrop yields had significant negative correlations with stand basal area of Paulownia elongata. We propose that adaptive tree-screening trials evaluate competitiveness in addition to evaluating growth and mortality. Stand basal area may be better suited to this task than more mechanistic indices such as leaf-area index as it is easy to measure, calculate, and understand, and it may serve as a better index of total (aboveground + belowground) competition. Basal area is also directly related to tree volume, and allows farmers to more easily evaluate the economic tradeoffs between tree growth and intercrop-yield declines. This revised version was published online in June 2006 with corrections to the Cover Date.     

我国泡桐抗丛枝病育种研究进展

从选种育种、引种和人工杂交育种三个方面介绍了我国的泡桐抗丛枝病育种方面所取得的成就，并阐述了目前泡桐抗病育种工作中存在的问题和今后的发展方向。     

不同种源泡桐种子场圃发芽率差异分析

为了探讨各种源泡桐生长适应情况,采用不同种源泡桐种子育苗,观察研究了其场圃发芽特性。结果表明:对于毛泡桐的种子潍坊种源发芽率最高,平均发芽时间与同种相比也较短;对于兰考泡桐的种子检验指标都显示出了宝鸡种源的优势;白花泡桐的种子的试验表明,福州种源的发芽率最高,但平均发芽时间也是最长,而南平种源的发芽率和发芽势仅次于福州种源,但它的平均发芽时间却比福州种源短的多,因此应考虑南平种源的栽培选育。     

用活性染料对泡桐单板仿红木进行染色的影响因素

采用活性染料对预处理后的泡桐单板进行了仿红木染色研究.结果表明:活性染料对泡桐有较好的易染性,泡桐单板经染色后能达到红木商业用材的自然效果;活性染料染色时,由于上染和固色是分开进行的,固色前染料可以充分进行扩散移染,从而有效避免泡桐单板表芯色差现象;通过控制染色温度、时间以及促染剂和固色剂用量,可得到满意的染色效果.试验得出的染色工艺条件为:温度70~80℃,时间3 h,促染剂元明粉40 g/L,固色剂纯碱20 g/L.     

泡桐属植物育种值预测方法的研究

分别用最佳线性无偏预测和最佳线性预测方法预测了泡桐属植物的育种值。结果表明，根据亲缘相关距阵对育种值的最佳线性无偏预测结果没有显著影响：数据量较大时，可用小区平均值取代单株值，同时也可用最佳线性预测代替最佳线性无偏预测方法预测育种值；预测育种值与真正育种值之间的相关系数与预测育种值之间的方法或误差方差存在极显著的相关关系，可用前者作预测育种值的精度指标。通过比较毛泡桐种源所预测育种值之间的方差和误差方差可以得出：在对研究材料评选时，最佳线性预测比最小平方估算法具有优越性，但当重复数大于5次，每个小区内的植株多于6时，这两种预测方法的精度非常接近。