液相色谱-串联质谱法测定水产品中孔雀石绿的残留量

建立了液相色谱-串联质谱测定水产品中孔雀石绿及其代谢物残留的检测方法。样品经乙酸铵盐溶液和乙腈提取,经二氯甲烷萃取,PRS阳离子交换柱净化,然后选用电喷雾离子源,在正离子、多反应监测方式模式下进行定性,以内标法进行定量。方法的检出限为0.10μg·kg-1,工作曲线的线性范围为0.10~4.00μg·kg-1。在添加浓度为0.10~4.00μg·kg-1的范围内,孔雀石绿和隐色孔雀石绿的平均回收率均分别为83.4%和95.0%,相对标准偏差分别为6.23%和6.74%。     

Phage and MLVA Typing of Salmonella Enteritidis Isolated from Layers and Humans in Belgium from 2000–2010, A Period in which Vaccination of Laying Hens was Introduced

The aim of the study was to characterize isolates of Salmonella enterica serovar Enteritidis (S. Enteritidis) obtained from humans and layer farms in Belgium collected during 2000–2010. Three periods were compared, namely (i) before implementation of vaccination (2000–2004), (ii) during voluntary vaccination (2005–2006) and (iii) during implementation of the national control program (NCP) for Salmonella including mandatory vaccination against S. Enteritidis (2007–2010). The characteristics compared across time periods were distributions of phage type and multiple‐locus variable number tandem‐repeat assay (MLVA). While PT4 and PT21 were predominantly isolated in Belgium in layers and humans before 2007, a significant reduction of those PTs was observed in both populations in the period 2007–2010. The relative proportion of PT4b, PT21c and PT6c was found to have increased considerably in the layer population since 2007. In the human population, PT8, PT1 and the group of ‘other’ PTs were more frequently isolated compared to the previous periods. When comparing the proportion of the predominant MLVA types Q2 and U2, no significant difference was found between the layer and human population in the three periods and between periods within each category (layer and human). A significant difference in isolate distribution among MLVA clusters I and II was found between human and layer isolates recovered during Period 3 and in the human population between Period 1 and 3. Results suggest that the association between S. Enteritidis in layers and the occurrence of the pathogen in humans changed since implementation of the NCP in 2007.     

Multiple‐Aetiology Enteric Infections Involving Non‐O157 Shiga Toxin‐Producing Escherichia coli – FoodNet, 2001–2010

We describe multiple‐aetiology infections involving non‐O157 Shiga toxin‐producing Escherichia coli (STEC) identified through laboratory‐based surveillance in nine FoodNet sites from 2001 to 2010. A multiple‐aetiology infection (MEI) was defined as isolation of non‐O157 STEC and laboratory evidence of any of the other nine pathogens under surveillance or isolation of >1 non‐O157 STEC serogroup from the same person within a 7‐day period. We compared exposures of patients with MEI during 2001–2010 with those of patients with single‐aetiology non‐O157 STEC infections (SEI) during 2008–2009 and with those of the FoodNet population from a survey conducted during 2006–2007. In total, 1870 non‐O157 STEC infections were reported; 68 (3.6%) were MEI; 60 included pathogens other than non‐O157 STEC; and eight involved >1 serogroup of non‐O157 STEC. Of the 68 MEI, 21 (31%) were part of six outbreaks. STEC O111 was isolated in 44% of all MEI. Of patients with MEI, 50% had contact with farm animals compared with 29% (P < 0.01) of persons with SEI; this difference was driven by infections involving STEC O111. More patients with non‐outbreak‐associated MEI reported drinking well water (62%) than respondents in a population survey (19%) (P < 0.01). Drinking well water and having contact with animals may be important exposures for MEI, especially those involving STEC O111.     

Growth testing of two breeds of common carp, Cyprinus carpio L. (Hungarian mirror and Třeboň scaly carp), in ponds with low and high stocking density

The growth of two breeds of common carp, Cyprinus carpio L., was tested in ponds under the climatic conditions of South Bohemia. T?eboň scaly carp (TR) and Hungarian mirror carp (M2) were kept in both low and high stocking densities during the second growing season and then stocked together for communal testing during the third growing season. Before the communal testing, the mean initial weights of fish from low‐ and high‐density stocks differed significantly (374.1 vs. 227.7 g for the TR breed and 766.7 vs. 317.3 g for the M2 breed respectively, P<0.01). After communal testing, mean weights of fish from low‐ and high‐density stocks gained 761.8 vs. 543.8 g for the TR breed and 1339.7 vs. 706.7 g for the M2 breed respectively. These observed weights were also significantly different (P<0.01). However, the test of corrected weight gain, i.e. gain not related to the initial weight of fish, revealed insignificant differences (P>0.01) between the weight gains after correction, i.e. the effect of different initial weights was successfully eliminated. These results seem to confirm the applicability of this method for the assessment of growth of purebred common carp under the climatic conditions of Central European fish farms.     

Involvement of steroid hormones in the preovulatory gonadotropin surge in female goldfish

Goldfish,Carassius auratus, spawn several times within a spawning season. A gonadotropin (GtH) surge occurs at the time of ovulation in this cyprinid species. This ovulatory GtH surge mediates the processes of final oocyte maturation and ovulation, and occurs at the end of each spawning cycle. Within a cycle, there is a shift in the predominant plasma steroid from estradiol to testosterone, and finally 17α, 20β-dihydroxy-4-pregnen-3-one at the time of the ovulatory GtH surge. High levels of testosterone were always observed before ovulation. When ovariectomized or sexually regressed female goldfish were implanted with testosterone, they exhibited a GtH surge which was similar to those normally observed at ovulation. These results strongly suggest that elevated plasma testosterone is an important physiological requirement for the occurrence of the GtH surge.     

含结构体工程堆积体土壤侵蚀研究

为探究野外实际调查中常见的含结构体工程堆积体土壤侵蚀过程,设计含结构体工程堆积体和对照组2种试验材料（对照组为不含结构体工程堆积体,后文中对2种试验材料简称为结构体和堆积体）,通过室内模拟降雨试验,研究了结构体和堆积体坡面径流侵蚀特征与雨强和场次的关系。结果表明：（1）初产历时随雨强和场次的增加而减小,结构体对初产历时有延缓作用,这与结构体的土壤特性和下垫面特征有关;（2）平均径流率和平均流速均随雨强和场次的增加而增大,堆积体平均流速和平均径流率分别是结构体的1.11~1.22,1.11~1.37倍,而结构体流速和径流率快速增加和趋于稳定的时间均较堆积体提前,且用时更短;（3）雨强对侵蚀速率、流速和径流率的贡献率较大,场次与侵蚀速率负相关,各条件下结构体的侵蚀速率均大于堆积体,且侵蚀速率和总侵蚀量分别是堆积体的1.03~2.15,1.36~2.63倍;（4）径流功率能够更好地描述结构体和堆积体侵蚀动力过程,结构体发生侵蚀的临界径流剪切力和径流功率均小于堆积体。     

白木香2种外植体的组织培养

沉香是国际上极负盛名的药用和香料资源之一。在亚洲许多国家,沉香产业既是传统行业也是发展迅猛的新兴产业。沉香优良种质的大规模应用对沉香产业的可持续发展具有重大意义。因此优良沉香种质资源是近年沉香产业关注的重点和热点,但一些优良种质的品质特性无法通过有性繁殖遗传,大规模繁育存在技术障碍。植物组织培养技术在品种优良性状保持、种子资源保存、快速繁殖等方面具有非常突出的优越性,被广泛应用于中药资源保护和中药产业发展。为建立工业化生产的沉香广谱再生体系,本文以白木香无菌苗和成龄植株的枝条为材料从外植体消毒、丛生芽诱导和生根培养三方面进行组织培养研究。结果表明,通过0.1%多菌灵溶液浸泡3 min,流动水冲洗3 h处理,对采摘自大田的白木香枝条有较好的除菌效果。0.1%升汞溶液消毒6~8 min,可有效保持外植体的成活率。在培养基中适当添加灭菌剂也能有效控制材料染菌。无菌苗茎段丛生芽诱导较易,大田枝条的丛生芽诱导较难。WPM + 20 g/L蔗糖+0.5 mg/L 6-BA+0.2 mg/L NAA (G₄)有利于无菌苗的丛生芽诱导;1/2 MS+25 g蔗糖+2 mg/L 6-BA+0.01 mg/L NAA (I₃),1/4 MS+20 g/L蔗糖+ 2 mg/L 6-BA+0.5 mg/L NAA (J₂)和WPM+20 g蔗糖+1 mg/L 6-BA+0.01 mg/L NAA (K₂)有利于成龄植株枝条外植体的丛生芽诱导,将膨大的丛生芽团切割后转接于不含植物生长调节剂的WPM培养基中可促进丛生芽伸长。WPM培养基中添加10~20 g/L蔗糖,并加入0.1~0.5 mg/L NAA可诱导无菌苗和茎段外植体的新芽生根。本研究以沉香2种外植体进行组织培养,成功获得再生植株,为沉香优良种质的无性繁殖提供技术参考。     

珍贵阔叶树种多树种混交造林技术

针对中国南方地区人工林树种林种单一、稳定性较差、物种多样性低,以至于生态服务功能低下,优质森林资源储备不足的问题,从改善林种结构,提高林分单位面积产量及生态效益出发,开展了南方主要珍贵阔叶树种多树种混交造林技术研究。结果表明,采用多树种混交模式,树种生长显著加快,单位面积总蓄积量增加,且在促进林木胸径生长方面效果显著,有利于大径材的培育;此外,多树种混交能够降低凋落物层C/N,养分分解转化快,生态稳定性更强。因此,珍贵树种多树种混交栽培模式具有可行性。     

珍贵阔叶树种多树种混交造林生长比较研究

为系统总结珍贵阔叶树种多树种混交造林技术,本试验对2种立地条件下的多树种混交林的生长情况进行了对比研究,结果表明：（1）珍贵树种多树种混交模式在立地条件较好的苗圃地生长相对较好,其中柚木（Tectonagrandis）的树高、胸径生长量均达到显著水平,降香黄檀（Dalbergiaodorifera）、观光木（Tsoongio?dendronodorum）以及红锥（Castanopsishystrix）则无显著差异,且林分总体材积蓄积量无显著差异,表明珍贵树种多树种混交模式在试验中两种立地条件下均能够表现出较好的适应性。（2）采用综合权重分析法评价树种的速生效果结果表明,以观光木速生效果较佳,按速生效果从高到低排序为：观光木＞柚木＞灰木莲（Man?glietiaglanca）＞红锥＞降香黄檀＞格木（Erythrophleumfordii）。