农杆菌介导遗传转化在水稻基因工程育种中的应用

随着生物技术的发展，水稻基因工程育种在水稻育种中发挥着越来越重要的作用。在诸多的转基因方法中，农杆菌作为一种天然的植物基因转化系统，以其独特优点而倍受重视。本文概述了农杆菌介导转化水稻的原理，农杆菌介导转化水稻的优点并且介绍了农杆菌菌株、受体材料和培养基成分的不同对农杆菌介导转化水稻的影响。另外对农杆菌介导遗传转化在水稻抗虫基因工程育种、抗病基因工程育种、抗逆基因工程育种、优质基因工程育种、耐除草剂基因工程育种及提高水稻光合效率和延缓衰老方面的研究进展进行了综述。最后对农杆菌介导遗传转化在水稻基因工程育种中存在的问题和发展前景做了简要说明。     

Biocompatibility of Acellular Porcine Dermis

In vitro cultured vascular endothelial cells (VEC) and mouse 3T3 fibroblasts (3T3) on the acellular dermal matrix , which were made of porcine skins. We made the cell proliferation test with MTT assay and the histological observation after cells were seeded on the acelluar dermis for 1 week with histological section. The cell growth curves showed VEC and 3T3 grew much rapidly on the acelluar dermis.The histological observation revealed VEC had formed a monolayer, some places even had formed 2 to 3 layer. The results suggest that the acelluar porcine dermal matrix have good biocompatibility , it will be widely applied.     

Deciding Method of Capability Vs. Price of Real Estate

The authors determine the index system and the index weight of capability of real estate by applying Delphi method and AHP method, and found the fuzzy comprehensive evaluation model of capability of real estate. They creates the model of capability Vs. price based on value engineering theory. The authors analysis detailedly deciding method of capability Vs. price of real estate combined with a practice example and application. The method adapts to a house as well as the type of the house. The method resolves the problem which capability Vs. price of real estate was only described by qualitatively for ages, which supplies theory basis to developer and consumer.     

The Essential Procedure of Numerical Simulation for Hydrodynamic in River and Coastal Engineering

Hydrodynamic models have been widely applied to river and coastal engineering projects. Understanding the hydrodynamic process is a prerequisite to modeling the pollution and sediment transport process. Hydrodynamic models of varying degrees of complexity should be selected appropriately according to the object of study, physical properties of the flow domain and hydrodynamic features. The essential procedure of hydrodynamic simulation is described in this paper. The main problems such as selection of model dimension in physical space, choice of variables, boundary conditions, initialization, design of computational grid, model resolution, parameterization of smaller scale fluctuations and sub-grid scale processes, model calibration and verification are investigated.     

Application and Advancement of Reinforced Earth Technique

In this paper, the application of reinforced earth technique, principle and calculation method, new reinforced materials developed and produced are introduced. A few of key technical problems in reinforced earth application is discussed, such as reinforced earth behavior, calculation method, durability of reinforced material, amendment and complement of the current standards. Then, the reinforced earth technique development and advancement were introduced.     

Enterprise Information Engineering Model and Its Integrated Support Environment

By analyzing the process of enterprise information engineering construction and intra relationship among information system requirement, business process modeling, application system constructing and project management, one of 4 meta model composed of requirement engineering, process engineering, application system engineering and support engineering is proposed for enterprise information engineering. The developments and applications of the business processes and its application systems are integrated into the same development space and application space. Basing on this, a prototype system composed of requirement modeling environment, business process modeling environment, application system modeling environment, and support engineering environment has been designed and built, which is used for supporting enterprise information engineering constructing. It is valued for the enterprise information engineering construction in our country.     

Risk Decision-making Method of Slope Resistant Engineering

Considering the character of slope resistant engineering invest benefit, in line with security and economy balmaced, combining initial investment and anticipated hazard loss, disaster resistant engineering should be satisfied with the criterion of minimum cost. Decision tree model is adopted as analyzing means, and risk decision-making of slope design is carried out. Project input-output radio adopting dynamic analysis can reflect project investment benefit more accurately. At last this paper analyzes some questions in process of slope resistant engineering with benefits enumerate.     

Analysis of Cleanroom Technology Through Angle of Design

During the implementation of Cleanroom Model, there are a lot of particular problems need to be resolved, which make it indispensable to grasp the essence and the principles of CRM. The article is on the essential research work in the design procedure of the Cleanroom technology. Through the analysis, it proposes a general architecture, based on which the entry point of the solving process and the distribution of the reducing procedure are introduced.     

Application of Function Analysis in Conceptual Design

Product design is a procedure that enterprises meet the needs of consumers; conceptive design is one of the steps in product design and it directly considers requirements of consumers. So how to supply consumers with satisfying function is the essential point. On the basis of function analysis of Value Engineering, the paper puts forward a new method to exercise function definition and function analysis, implements conceptual product design procedure with database techniques.A new way is gotten to convert requirements to conceptual product.