High frequency embryogenic callus induction and plant regeneration from mature caryposis of big bluestem and little bluestem

Big bluestem (Andropogon gerardii Vitman) and little bluestem [Schizachyrium scoparium (Michaux) Nash.] are native to the North America and are important forage grasses and ornamental grasses. Both grasses are proposed as ideal biomass producers for cellulosic ethanol production. To apply genetic transformation, which is an important tool for incorporating desirable agronomic traits into plants to both species, however requires an efficient and reproducible regeneration protocol. We used mature caryopses from big and little bluestem as explants and tested the effect of various combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) (1, 2, 3, 4 or 5 mg l⁻¹) and kinetin (KT) (0, 0.1 or 0.2 mg l⁻¹) on embryogenic callus induction with LS as the basal medium. The highest percentage of embryogenic calli induction occurred on medium containing 2, 4-D alone at 2 mg l⁻¹ for ‘Bison’ and on medium containing 4 mg l⁻¹ 2, 4-D alone for ‘Bonilla’ big bluestem. For little bluestem, the highest percentage of embryogenic callus induction occurred on medium containing 3 mg l⁻¹ 2, 4-D plus 0.1 mg l⁻¹ kinetin, suggesting that addition of KT is beneficial. Shoot regeneration took place on LS basal medium without any plant growth regulator for both species, although the addition of KT increased both regeneration frequency and the number of shoots produced per callus. Rooting of shoots reaching about 2 cm long occurred readily with or without α-naphthaleneacetic acid (NAA). Rooted plantlets were all successfully established in the soil.     

Boundary-fitted grid in landscape modeling

Landscape modeling requires the delineation of system boundaries and interior features. Quite often, these components are complex and difficult to accurately represent. A rectangular grid is used to represent the study and adjacent non-study areas in most cases. When the non-study area occupies a large portion of the grid, computer memory is wasted, and computational time increases. An elliptical grid generator for non-orthogonal curvilinear coordinates is used to generate a boundary-fitted grid for a landscape model. In a boundary-fitted grid coordinate system, one coordinate axis follows the landscape domain boundary and is non-orthogonal to the second axis. The boundary-fitted grid uses elliptic partial differential equations to distribute grid points inside the landscape domain. Although the boundary-fitted grid follows the domain boundary, the grid pattern and point allocation remain structured. Thus, a landscape model can use a boundary-fitted grid without changing the model’s data structure or the computational scheme. In this study, a boundary-fitted grid and a raster-based grid were applied to the Everglades Landscape Fire Model. Use of the boundary-fitted grid decreased model simulation time by about one fifth and computer storage by 58% relative to the raster-based grid. Also, the linear characteristics of interior geographical features such as rivers and airboat trails were preserved by the boundary-fitted grid, but not by the raster-based grid. This preservation provided a more reasonable base map for simulating ecological processes, such as fire across heterogenous landscapes.     

Some Comments on Gerechte Designs

In a companion paper we derived the correct analysis for gerechte designs with uncorrected errors. Here we show that this correct analysis cannot be justified by the usual randomization argument. However, when the regions are rectangular there is a randomization procedure which validates an analysis with three separate error terms. We also outline other developments in design and analysis that may be more satisfactory.     

杉木扁长蝽若虫空间分布型的研究

本文采用聚集度指标检验法、回归分析法研究了杉木扁长蝽若虫的空间分布型。结果表明：在不同林地、不同虫口密度情况下，均呈聚集分布。若虫的聚集与栖息生境有直接关系。     

Nisin 对 Lactobacillus acidophilus La-5 和 Bifidobac

在MRS平板上，采用琼脂扩散法测定了Nisin对Lactobacillus acidophilus La-5和Bifidobacterium bifidum Bb-12生长的抑制作用。当Nisin浓度≥50ug/ml时，对Lactobacillus acidophilus La-5表现出强烈的抑制作用，而Bifidobacterium bifidum Bb-12则几乎不生长，即使Nisin的浓度仅为25ug/ml，当L.acidophilus La-5和B.bifidum Bb-12单独或两者共同在37℃发酵10％（w/w)还原脱脂奶时，脱脂奶中添加的50ug/ml Nisin对B.bifidum Bb-12表现出杀菌作用，而对L.acidophilus La-5则为抑菌作用。当延长培养时间后。L.acidophilus La-5的存在可以降低Nisin对B.bifidum Bb-12的致死作用。     

13～18周龄笼养蛋鸭适宜蛋白质和蛋氨酸水平的研究

用 32 4羽满 1 2周龄的金定鸭 ,通过饲养试验、代谢试验、屠宰试验和对试鸭产蛋期 (1 9～ 2 8周龄 )产蛋性能的观察 ,研究了在等能条件下(ME :1 1 51MJ/kg) ,不同蛋白质水平 (CP :1 3 %、 1 5 %、 1 7% )和蛋氨酸水平 (Met/CP :0 0 1 7、 0 0 2 2、 0 0 2 7)组成的 9种饲粮对 1 3～ 1 8周龄笼养蛋鸭的生长性能、养分利用、体成分沉积和产蛋期产蛋性能的影响。结果表明 :该阶段饲粮粗蛋白、蛋氨酸水平对体增重影响不显著 (P >0 0 5) ;体增重与蛋氨酸 (% )呈一定的正相关 (r=0 4574) ;CP为 1 3%、Met/CP为 0 0 2 7组采食量一直较稳定、较高 ,且饲料效率又好。代谢试验 :不同粗蛋白、蛋氨酸水平饲粮对试鸭氮存留率和能量的利用率影响显著(P <0 0 5或P<0 0 1 ) ,且它们随饲粮CP水平的升高而降低 ,随蛋氨酸水平升高而升高 ;磷的利用率也分别以CP为 1 3%的水平和Met/CP为 0 0 2 7的水平极显著地高于其他水平对磷利用率 (P <0 0 1 )。屠宰试验 :当Met/CP为 0 0 2 7时 ,体氮沉积量和体磷沉积量最佳。产蛋性能 :开产日龄和高峰日龄与 1 3～ 1 8周龄CP食入量呈弱的负相关 (r=- 0 1 83 3和r=- 0 0 984) ,与蛋氨酸食入量呈正相关 (r =0 5780和r =0 435 0 )。饲粮粗蛋白、蛋氨酸水平对 1 9～ 2 8周龄     

Relating Kirtland's warbler population to changing landscape composition and structure

The population of male Kirtland's warbler (Dendroica kirtlandii) in the breeding season has averaged 206 from 1971 to 1987. The Kirtland's warbler occupies dense jack pine (Pinus banksiana) barrens from 5 to 23 years old and from 1.4 to 5.0 m high, formerly of wildfire origin. In 1984, 73% of the males censused were found in habitat naturally regenerated from wildfire or prescribed burning. The rest were in plantations (11%) or in harvested, unburned jack pine stands stocked by natural regeneration (16%). Twenty-two percent (630 of 2,886) of the Kirtland's warbler males counted in the annual censuses from 1971 through 1984 were found in 26 stands that were unburned and naturally regenerated following harvest. From 1982 to 1987, suitable regenerating areas were barely sufficient to replace currently occupied maturing stands, so population growth was impeded. Ecosystems of suitable size and regeneration characteristics (wildfire and plantation) doubled in area by 1989. In response, the population of Kirtland's warblers increased from 167 to 398 males between 1987 and 1992, but they withdrew almost entirely from the unburned, unplanted barrens by 1989 when the area of more suitable regeneration types increased. Minimum (368 males) and maximum (542 males) population estimates for 1996 were calculated based on 1984 average density (1.9 males per 40 ha) and peak population in burns (2.8 males per 40 ha).     

辽宁西部半干旱地区油松纯林改造的研究

喀左县位于辽宁西部地区，现存大面积油松人工纯林，不仅改善地力和水土保持效益差，而且火险性强、虫害蔓延成灾、生长早期衰退。为改善其林分生态条件，提高林木生长量和综合效益，在中三家镇对25年生油松人工纯林进行改造试验。结果表明，选择山杏、刺槐、沙棘等乔、灌木树种，利用林间空地、林缘隙地、侵蚀沟壑和疏林地造林，在大面积油松纯林连片地区进行窄带状或块状砍伐后造林，5年后效益显著，林分相对湿度提高了2．2％，土壤有机质含量提高5．4％，土壤含水量提高9．8％，减轻土壤容重３％,林分虫害明显下降，油松树高、胸径生长量分别提高22．6％、19．8％。     

山地橄榄主要病虫害种类及其防治措施

介绍了山地橄榄2种病害：叶斑病、炭疽病；5种虫害：橄榄星室木虱、黑刺粉虱、恶性叶甲、橄榄枯叶蛾、橄榄野螟的主要特征和危害症状，并提出了防治措施。     

Importance of the River Teviot to Atlantic salmon, Salmo salar, rod catches in the River Tweed, Scotland

Abstract  The body mass distributions and variations in abundance of the spring and autumn catches of Atlantic salmon, Salmo salar L., were compared between fisheries above and below the confluence of the rivers Tweed and Teviot, Scotland. Significant differences between the distributions were found over three periods, one in spring and two in autumn, when salmon catches were high. Correlations were found between abundance trends for periods when distributions were similar or dissimilar. Abundance of salmon returning to the River Teviot was proportional to the main river. However, its population structure differed when abundances were high, suggesting different survival strategies. It appears that changes in the numbers returning to the river were resultant of changes in the marine rather than freshwater environment.